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anti-LCK antibody :: Rabbit LCK (pY393) Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS822038
Immunofluorescence/Immunocytochemistry (IF/ICC)
Unit / Price
0.03 mL  /  $165 +1 FREE 8GB USB
0.1 mL  /  $245 +1 FREE 8GB USB
0.2 mL  /  $350 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

LCK (pY393), Polyclonal Antibody

★Popular Item★
 Also Known As   

Anti-LCK (pY393) Antibody

 Product Synonym Names    Tyrosine-protein kinase Lck; Leukocyte C-terminal Src kinase; LSK; Lymphocyte cell-specific protein-tyrosine kinase; Protein YT16; Proto-oncogene Lck; T cell-specific protein-tyrosine kinase; p56-LCK
 Product Gene Name   

anti-LCK antibody

[Similar Products]
 Antibody/Peptide Pairs    LCK (pY393) peptide (MBS823865) is used for blocking the activity of LCK (pY393) antibody (MBS822038)
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    gene 615758
 3D Structure    ModBase 3D Structure for P06239
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 Clonality    Polyclonal
 Host    Rabbit
 Species Reactivity    Human, Mouse, Rat, Bovine, Chicken, Pig, Sheep
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 Specificity    Recognizes endogenous levels of LCK (pY393) protein.
 Purity/Purification    The antibody was purified by immunogen affinity chromatography.
 Form/Format    Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
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 Immunogen    KLH-conjugated synthetic peptide encompassing a sequence within the center region of human LCK. The exact sequence is proprietary.
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 Preparation and Storage    Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
 Other Notes    Small volumes of anti-LCK antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-LCK antibody

   Rabbit polyclonal antibody to LCK (pY393)
 Applications Tested/Suitable for anti-LCK antibody   

Western Blot (WB), Immunofluorescence (IF), Immunocytochemsitry (ICC)

 Application Notes for anti-LCK antibody    WB (1/500 - 1/1000), IF/ICC (1/100 - 1/500)
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 Western Blot (WB) of anti-LCK antibody    anti-LCK antibody Western Blot (WB) (WB) image
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 Immunofluorescence/Immunocytochemistry (IF/ICC) of anti-LCK antibody    Immunofluorescent analysis of LCK (pY393) staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 degree C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
anti-LCK antibody Immunofluorescence/Immunocytochemistry (IF/ICC) (ICC) image
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NCBI/Uniprot data below describe general gene information for LCK. It may not necessarily be applicable to this product.
 NCBI GI #    112789548
 NCBI GeneID    3932
 NCBI Accession #    NP_001036236.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001042771.2 [Other Products]
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 UniProt Primary Accession #    P06239 [Other Products]
 UniProt Secondary Accession #    P07100; Q12850; Q13152; Q5TDH8; Q5TDH9; Q7RTZ3; Q96DW4; Q9NYT8; D3DPP8 [Other Products]
 UniProt Related Accession #    P06239 [Other Products]
 Molecular Weight    61,190 Da
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 NCBI Official Full Name    tyrosine-protein kinase Lck
 NCBI Official Synonym Full Names    LCK proto-oncogene, Src family tyrosine kinase
 NCBI Official Symbol    LCK [Similar Products]
 NCBI Official Synonym Symbols   
LSK; YT16; IMD22; p56lck; pp58lck
[Similar Products]
 NCBI Protein Information    tyrosine-protein kinase Lck; leukocyte C-terminal Src kinase; p56(LSTRA) protein-tyrosine kinase; t cell-specific protein-tyrosine kinase; proto-oncogene tyrosine-protein kinase LCK; lymphocyte-specific protein tyrosine kinase; lymphocyte cell-specific protein-tyrosine kinase; T-lymphocyte specific protein tyrosine kinase p56lck
 UniProt Protein Name    Tyrosine-protein kinase Lck
 UniProt Synonym Protein Names   
Leukocyte C-terminal Src kinase; LSK; Lymphocyte cell-specific protein-tyrosine kinase; Protein YT16; Proto-oncogene Lck; T cell-specific protein-tyrosine kinase; p56-LCK
 Protein Family    Tyrosine-protein kinase
 UniProt Gene Name    LCK [Similar Products]
 UniProt Synonym Gene Names    LSK [Similar Products]
 UniProt Entry Name    LCK_HUMAN
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 NCBI Summary for LCK    This gene is a member of the Src family of protein tyrosine kinases (PTKs). The encoded protein is a key signaling molecule in the selection and maturation of developing T-cells. It contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. The protein localizes to the plasma membrane and pericentrosomal vesicles, and binds to cell surface receptors, including CD4 and CD8, and other signaling molecules. Multiple alternatively spliced variants, encoding the same protein, have been described. [provided by RefSeq, Jul 2008]
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 UniProt Comments for LCK    Lck: a tyrosine kinase of the Src family that is crucial to antigen-receptor signaling in lymphocytes. plays an essential role for the selection and maturation of developing T-cell in the thymus and in mature T-cell function. Is constitutively associated with the cytoplasmic portions of the CD4 and CD8 surface receptors and plays a key role in T-cell antigen receptor(TCR)-linked signal transduction pathways. Association of the TCR with a peptide antigen-bound MHC complex facilitates the interaction of CD4 and CD8 with MHC class II and class I molecules, respectively, and thereby recruits the associated LCK to the vicinity of the TCR/CD3 complex. LCK then phosphorylates tyrosines residues within the immunoreceptor tyrosines-based activation motifs (ITAMs) in the cytoplasmic tails of the TCRgamma chains and CD3 subunits, initiating the TCR/CD3 signaling pathway. In addition, contributes to signaling by other receptor molecules. Associates directly with the cytoplasmic tail of CD2, and upon engagement of the CD2 molecule, LCK undergoes hyperphosphorylation and activation. Also plays a role in the IL2 receptor-linked signaling pathway that controls T-cell proliferative response. Binding of IL2 to its receptor results in increased activity of LCK. Is expressed at all stages of thymocyte development and is required for the regulation of maturation events that are governed by both pre-TCR and mature alpha beta TCR. Binds to the cytoplasmic domain of cell surface receptors, such as CD2, CD4, CD5, CD8, CD44, CD45 and CD122. Also binds to effector molecules, such as PI4K, VAV1, RASA1, FYB and to other protein kinases including CDC2, RAF1, ZAP70 and SYK. Binds to phosphatidylinositol 3'-kinase (PI3K) from T-lymphocytes through its SH3 domain and to the tyrosine phosphorylated form of Sam68 through its SH2 domain. Binds to HIV-1 Nef through its SH3 domain. This interaction inhibits its tyrosine-kinase activity. Overexpression in mice leads to thymic tumors. Aberrant expression is seen in T cell leukemias and colon cancer. The leukemic translocation t(1;7)(p34;q34) has breakpoints at the T cell receptor gene and close to the Lck promoters, can cause increased Lck expression, and in one case, point mutations. A mutated Lck has also been seen in a cell line. One patient with aberrant Lck splicing suffered from SCID-like T cell deficiency. Inhibitor: BMS-279700. Three alternatively spliced isoforms of the human proteinhave been described.

Protein type: Kinase, protein; Protein kinase, tyrosine (non-receptor); EC 2.7.10.2; Protein kinase, TK; Oncoprotein; TK group; Src family

Chromosomal Location of Human Ortholog: 1p34.3

Cellular Component: pericentriolar material; extrinsic to internal side of plasma membrane; endocytic vesicle; plasma membrane; immunological synapse; intercellular junction; cytosol; lipid raft

Molecular Function: protein C-terminus binding; identical protein binding; CD8 receptor binding; non-membrane spanning protein tyrosine kinase activity; protein serine/threonine phosphatase activity; protein kinase binding; antigen binding; protein phosphatase binding; ATPase binding; protein binding; protein-tyrosine kinase activity; protein complex binding; phosphoinositide 3-kinase binding; SH2 domain binding; glycoprotein binding; CD4 receptor binding; ATP binding

Biological Process: nerve growth factor receptor signaling pathway; viral reproduction; protein amino acid phosphorylation; T cell receptor signaling pathway; B cell receptor signaling pathway; positive regulation of T cell receptor signaling pathway; hemopoiesis; positive regulation of gamma-delta T cell differentiation; T cell differentiation; aging; epidermal growth factor receptor signaling pathway; response to drug; caspase activation; regulation of lymphocyte activation; platelet activation; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; cellular zinc ion homeostasis; regulation of defense response to virus by virus; regulation of cell proliferation; positive regulation of tyrosine phosphorylation of Stat5 protein; response to hydrogen peroxide; dephosphorylation; response to mechanical stimulus; response to zinc ion; release of sequestered calcium ion into cytosol; T cell costimulation; innate immune response; positive regulation of T cell activation; blood coagulation; leukocyte migration; transmembrane receptor protein tyrosine kinase signaling pathway

Disease: Immunodeficiency 22
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 Research Articles on LCK    1. Lck is retained in the cytosol of CD222-deficient cells, which obstructs the recruitment of Lck to CD45 at the cell surface, resulting in an abundant inhibitory phosphorylation signature on Lck at the steady state.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with anti-LCK antibodyDiseases associated with anti-LCK antibody
 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Alpha-synuclein Signaling Pathway antibodies  Alpha-synuclein Signaling Pathway Diagram
 Atypical NF-kappaB Pathway antibodies  Atypical NF-kappaB Pathway Diagram
 B Cell Receptor Signaling Pathway antibodies  B Cell Receptor Signaling Pathway Diagram
 CD28 Co-stimulation Pathway antibodies  CD28 Co-stimulation Pathway Diagram
 CD28 Dependent PI3K/Akt Signaling Pathway antibodies  CD28 Dependent PI3K/Akt Signaling Pathway Diagram
 CD28 Dependent Vav1 Pathway antibodies  CD28 Dependent Vav1 Pathway Diagram
 CTLA4 Inhibitory Signaling Pathway antibodies  CTLA4 Inhibitory Signaling Pathway Diagram
 CXCR4-mediated Signaling Events Pathway antibodies  CXCR4-mediated Signaling Events Pathway Diagram
 Cell Surface Interactions At The Vascular Wall Pathway antibodies  Cell Surface Interactions At The Vascular Wall Pathway Diagram
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >63 publications with LCK and Inflammation
 Necrosis Antibodies  >55 publications with LCK and Necrosis
 Neoplasms, Experimental Antibodies  >49 publications with LCK and Neoplasms, Experimental
 Lung Diseases Antibodies  >37 publications with LCK and Lung Diseases
 Adenocarcinoma Antibodies  >30 publications with LCK and Adenocarcinoma
 Nervous System Diseases Antibodies  >23 publications with LCK and Nervous System Diseases
 Lung Neoplasms Antibodies  >19 publications with LCK and Lung Neoplasms
 Liver Diseases Antibodies  >19 publications with LCK and Liver Diseases
 Breast Neoplasms Antibodies  >18 publications with LCK and Breast Neoplasms
 Neoplasm Metastasis Antibodies  >14 publications with LCK and Neoplasm Metastasis
Organs/Tissues associated with anti-LCK antibody
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >1854 publications with LCK and Blood
 Thymus Antibodies  >288 publications with LCK and Thymus
 Spleen Antibodies  >115 publications with LCK and Spleen
 Lymph Node Antibodies  >62 publications with LCK and Lymph Node
 Lymph Antibodies  >59 publications with LCK and Lymph
 Lung Antibodies  >54 publications with LCK and Lung
 Brain Antibodies  >49 publications with LCK and Brain
 Liver Antibodies  >48 publications with LCK and Liver
 Heart Antibodies  >43 publications with LCK and Heart
 Vascular Antibodies  >38 publications with LCK and Vascular
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