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anti-LAMC2 antibody :: Rabbit anti-Human Laminin Gamma 2 Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS2027068 (SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
0.01 mg  /  $110 +1 FREE 8GB USB
0.02 mg  /  $130 +1 FREE 8GB USB
0.05 mg  /  $175 +1 FREE 8GB USB
0.1 mg  /  $225 +1 FREE 8GB USB
0.2 mg  /  $340 +1 FREE 8GB USB
1 mg  /  $800 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Laminin Gamma 2 (LAMC2), Polyclonal Antibody

★Popular Item★
 Also Known As   

Polyclonal Antibody to Laminin Gamma 2 (LAMC2)

 Product Gene Name   

anti-LAMC2 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Immunogen Sequence    The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- CDC NGKSRQCIFD RELHRQTGNG FRCLNCNDNT DGIHCEKCKN GFYRHRERDR CLPCNCNSKG SLSARCDNSG RCSCKPGVTG ARCDRCLPGF HMLTDAGCTQ DQRLLDSKCD CDPAGIAGPC DAGRCVCKPA VTGERCDRCR SGYYNLDGGN PEGCTQCFCY GHSASC
 OMIM    Z15008 mRNA
 3D Structure    ModBase 3D Structure for Q13753
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 Clonality    Polyclonal
 Isotype    IgG
 Host    Rabbit
 Species Reactivity    Human
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 Specificity    The antibody is a rabbit polyclonal antibody raised against LAMC2. It has beenselected for its ability to recognize LAMC2 in immunohistochemical staining andwestern blotting.
 Purity/Purification    Affinity Chromatography
 Form/Format    Liquid
 Concentration    200ug/ml (lot specific)
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 Fragment    LAMC2 (Cys28~Cys196)
 Organism Species    Homo sapiens (Human)
 Conjugate    No Conjugate
 Immunogen    Recombinant LAMC2 (Cys28~Cys196) expressed in E Coli.
 Conjugated Antibody    The APC conjugated antibody version of this item is also available as catalog #MBS2062921
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 Preparation and Storage    Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
 Other Notes    Small volumes of anti-LAMC2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Product Categories/Family for anti-LAMC2 antibody    Knock-Out Validation Antibody
 Applications Tested/Suitable for anti-LAMC2 antibody   

Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)

 Application Notes for anti-LAMC2 antibody    Western blotting: 0.2-2ug/mL;1:250-2500
Immunohistochemistry: 5-20ug/mL;1:25-100
Immunocytochemistry: 5-20ug/mL;1:25-100
Optimal working dilutions must be determined by end user.
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 Western Blot (WB) Knockout Verification of anti-LAMC2 antibody    Knockout Verification: Lane 1: Wild-type A431 cell lysate; Lane 2: LAMC2 knockout A431 cell lysate; Predicted MW: 121,130kd Observed MW: 120kd Primary Ab: 5ug/ml Rabbit Anti-Human LAMC2 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
anti-LAMC2 antibody Western Blot (WB) Knockout Verification (WB) image
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 Western Blot (WB) of anti-LAMC2 antibody    Western Blot; Sample: Human Lung lysate; Primary Ab: 5ug/ml Rabbit Anti-Human LAMC2 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
anti-LAMC2 antibody Western Blot (WB) (WB) image
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 Western Blot (WB) of anti-LAMC2 antibody    Western Blot; Sample: Recombinant protein.
anti-LAMC2 antibody Western Blot (WB) (WB) image
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 Immunohistochemistry (IHC) of anti-LAMC2 antibody    DAB staining on fromalin fixed paraffin-embedded Kidney tissue)
anti-LAMC2 antibody Immunohistochemistry (IHC) (IHC) image
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NCBI/Uniprot data below describe general gene information for LAMC2. It may not necessarily be applicable to this product.
 NCBI GI #    157419138
 NCBI GeneID    3918
 NCBI Accession #    NP_005553.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_005562.2 [Other Products]
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 UniProt Primary Accession #    Q13753 [Other Products]
 UniProt Secondary Accession #    Q02536; Q02537; Q13752; Q14941; Q14DF7; Q2M1N2; Q5VYE8 [Other Products]
 UniProt Related Accession #    Q13753 [Other Products]
 Molecular Weight    121,604 Da
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 NCBI Official Full Name    laminin subunit gamma-2 isoform a
 NCBI Official Synonym Full Names    laminin subunit gamma 2
 NCBI Official Symbol    LAMC2 [Similar Products]
 NCBI Official Synonym Symbols   
B2T; CSF; EBR2; BM600; EBR2A; LAMB2T; LAMNB2
[Similar Products]
 NCBI Protein Information    laminin subunit gamma-2
 UniProt Protein Name    Laminin subunit gamma-2
 UniProt Synonym Protein Names   
Cell-scattering factor 140 kDa subunit; CSF 140 kDa subunit; Epiligrin subunit gamma; Kalinin subunit gamma; Kalinin/nicein/epiligrin 100 kDa subunit; Ladsin 140 kDa subunit; Laminin B2t chain; Laminin-5 subunit gamma; Large adhesive scatter factor 140 kDa subunit; Nicein subunit gamma
 Protein Family    Laminin
 UniProt Gene Name    LAMC2 [Similar Products]
 UniProt Synonym Gene Names    LAMB2T; LAMNB2; CSF 140 kDa subunit [Similar Products]
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 NCBI Summary for LAMC2    Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins, composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively), have a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. Several isoforms of each chain have been described. Different alpha, beta and gamma chain isomers combine to give rise to different heterotrimeric laminin isoforms which are designated by Arabic numerals in the order of their discovery, i.e. alpha1beta1gamma1 heterotrimer is laminin 1. The biological functions of the different chains and trimer molecules are largely unknown, but some of the chains have been shown to differ with respect to their tissue distribution, presumably reflecting diverse functions in vivo. This gene encodes the gamma chain isoform laminin, gamma 2. The gamma 2 chain, formerly thought to be a truncated version of beta chain (B2t), is highly homologous to the gamma 1 chain; however, it lacks domain VI, and domains V, IV and III are shorter. It is expressed in several fetal tissues but differently from gamma 1, and is specifically localized to epithelial cells in skin, lung and kidney. The gamma 2 chain together with alpha 3 and beta 3 chains constitute laminin 5 (earlier known as kalinin), which is an integral part of the anchoring filaments that connect epithelial cells to the underlying basement membrane. The epithelium-specific expression of the gamma 2 chain implied its role as an epithelium attachment molecule, and mutations in this gene have been associated with junctional epidermolysis bullosa, a skin disease characterized by blisters due to disruption of the epidermal-dermal junction. Two transcript variants resulting from alternative splicing of the 3' terminal exon, and encoding different isoforms of gamma 2 chain, have been described. The two variants are differentially expressed in embryonic tissues, however, the biological significance of the two forms is not known. Transcript variants utilizing alternative polyA_signal have also been noted in literature. [provided by RefSeq, Aug 2011]
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 UniProt Comments for LAMC2    LAMC2: Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Ladsin exerts cell- scattering activity toward a wide variety of cells, including epithelial, endothelial, and fibroblastic cells. Defects in LAMC2 are a cause of epidermolysis bullosa junctional Herlitz type (H-JEB); also known as junctional epidermolysis bullosa Herlitz-Pearson type. JEB defines a group of blistering skin diseases characterized by tissue separation which occurs within the dermo-epidermal basement membrane. H-JEB is a severe, infantile and lethal form. Death occurs usually within the first six months of life. Occasionally, children survive to teens. H-JEB is marked by bullous lesions at birth and extensive denudation of skin and mucous membranes that may be hemorrhagic. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Extracellular matrix; Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1q25.3

Cellular Component: cell cortex; extracellular region; extracellular space; laminin-2 complex; membrane; perinuclear region of cytoplasm

Molecular Function: heparin binding

Biological Process: cell adhesion; epidermis development; extracellular matrix disassembly; extracellular matrix organization; hemidesmosome assembly; positive regulation of cell migration; positive regulation of cell proliferation; system development

Disease: Epidermolysis Bullosa, Junctional, Herlitz Type; Epidermolysis Bullosa, Junctional, Non-herlitz Type
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 Research Articles on LAMC2    1. Overexpression of LAMC2 and knockdown of CD82 markedly promoted GC cell invasion and activated EGFR/ERK1/2-MMP7 signaling via upregulation of the expression of phosphorylated (p)-EGFR, p-ERK1/2 and MMP7.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-LAMC2 antibodyPathways associated with anti-LAMC2 antibody
 Reference Product  PubMed Publications
 LAMB3 antibody  >48 publications with LAMC2 and LAMB3
 COL7A1 antibody  >3 publications with LAMC2 and COL7A1
 BMP1 antibody  >1 publications with LAMC2 and BMP1
 Products by Pathway  Pathway Diagram
 Alpha6-Beta4 Integrin Signaling Pathway antibodies  Alpha6-Beta4 Integrin Signaling Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Cell Junction Organization Pathway antibodies  Cell Junction Organization Pathway Diagram
 ECM-receptor Interaction Pathway antibodies  ECM-receptor Interaction Pathway Diagram
 ECM-receptor Interaction Pathway antibodies  ECM-receptor Interaction Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 Inflammatory Response Pathway antibodies  Inflammatory Response Pathway Diagram
Diseases associated with anti-LAMC2 antibodyOrgans/Tissues associated with anti-LAMC2 antibody
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >98 publications with LAMC2 and Neoplasms
 Carcinoma Antibodies  >82 publications with LAMC2 and Carcinoma
 Skin Diseases Antibodies  >53 publications with LAMC2 and Skin Diseases
 EPIDERMOLYSIS BULLOSA, JUNCTIONAL, HERLITZ TYPE Antibodies  >50 publications with LAMC2 and EPIDERMOLYSIS BULLOSA, JUNCTIONAL, HERLITZ TYPE
 Genetic Diseases, Inborn Antibodies  >46 publications with LAMC2 and Genetic Diseases, Inborn
 Congenital Abnormalities Antibodies  >45 publications with LAMC2 and Congenital Abnormalities
 Carcinoma, Squamous Cell Antibodies  >41 publications with LAMC2 and Carcinoma, Squamous Cell
 Neoplasm Invasiveness Antibodies  >38 publications with LAMC2 and Neoplasm Invasiveness
 Head and Neck Neoplasms Antibodies  >31 publications with LAMC2 and Head and Neck Neoplasms
 Adenocarcinoma Antibodies  >28 publications with LAMC2 and Adenocarcinoma
 Organ/Tissue Name  Pubmed Publications
 Skin Antibodies  >52 publications with LAMC2 and Skin
 Mouth Antibodies  >21 publications with LAMC2 and Mouth
 Lung Antibodies  >13 publications with LAMC2 and Lung
 Lymph Node Antibodies  >10 publications with LAMC2 and Lymph Node
 Embryonic Tissue Antibodies  >9 publications with LAMC2 and Embryonic Tissue
 Bone Antibodies  >7 publications with LAMC2 and Bone
 Nerve Antibodies  >7 publications with LAMC2 and Nerve
 Liver Antibodies  >7 publications with LAMC2 and Liver
 Stomach Antibodies  >6 publications with LAMC2 and Stomach
 Eye Antibodies  >6 publications with LAMC2 and Eye
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