View our list of available Coronavirus (COVID-19, SARS-CoV-2, 2019-nCOV) antibodies, recombinant proteins, ELISA Kits... Options Full Datasheet Printer Friendly Datasheet Prepare for Printing Full Datasheet Printer Friendly Datasheet Prepare for Printing Have Questions? Ask Us! Request A Quote Today! Add To Compare List Rabbit M-2 Muscarinic receptor Polyclonal Antibody Catalog # MBS352155 Unit / Price 0.1 mL / $325 +1 FREE 8GB USB 5x0.1 mL / $1,335 +1 FREE 8GB USB Product Name Product Info Accession #s Product Desc Diseases/Tissues/Pathways Applications References Product Name M-2 Muscarinic receptor, Polyclonal Antibody Full Product Name Rabbit Anti-Muscarinic M2 Receptor Serum Product Synonym Names Muscarinic M 2 Receptor; M 2 Receptor (457-466) Research Use Only For Research Use Only. Not for use in diagnostic procedures. TOP Clonality Polyclonal Host Rabbit Specificity Polypeptide ::: % Cross ReactivityM1 Muscarinic Receptor (451-46) ::: 100M1 Muscarinic Receptor ::: 60 Form/Format Antiserum Specificity Other Notes Small volumes of anti-M-2 Muscarinic receptor antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply. TOP Related Product Information for anti-M-2 Muscarinic receptor antibody The antiserum was raised in a rabbit which was immunized with a peptide analogue of the carboxyl terminal of the muscarinic M2 receptor covalently attached onto a carrier protein. This antiserum is specific for the COOH terminal of the M2 receptor and is suitable for immunocytochemical and western immunoblotting detection of the receptor. The antiserum has been characterized by western blotting. ELISA. and cell staining techniques. Dilute the lyophilized antiserum with 0.1 ml of 10 mg/ml BSA is PBS for the equivalent of whole antiserum. or with additional buffer for more dilute antiserum. Product Categories/Family for anti-M-2 Muscarinic receptor antibody Histochemical Grade Antibodies; Muscarinic Receptor Antibodies and Kits Applications Tested/Suitable for anti-M-2 Muscarinic receptor antibody Immunocytochemical Staining, Western Immunoblot, Western Blot Application Notes for anti-M-2 Muscarinic receptor antibody Immunocytochemistry: This antiserum has been found to stain PLP fixed rat brain sections known to express the muscarinic M2 receptor using ABC techniques at a dilution of 1:5000.Western Blot: Western immunoblots using whole rat brain homogenate have been successful at a dilution of 1:1000.Western Blot Protocol: 1. After SDS-PAGE (on either 4-15% gradient gels or single percentage gels. such at 12% gels) and electrophoretic transfer to PVDF membrane. block the membrane overnight with 2% normal goat serum in TBS/Tween-20 buffer.2. Wash x 2 with TBS/Tween-20.3. Apply the rabbit polyclonal antibody after dilution to at least 1:1000 (Note: higher dilutions may be needed). Use 2% normal goat serum in TBS/Tween-20 as buffer for the primary antibody. Let the primary antibody bind for 2-4 hours.4. Wash x 3 with TBS/Tween-20.5. Apply affinity purified HRP-goat anti-rabbit IgG antiserum diluted 1:2500 (dilution may vary depending upon supplier) in 2% normal goat serum in TBS/Tween-20. Incubate 1-2 hours. Note: greater sensitivity may be achieved using ABC techniques.6. Wash x 4 for 5 minute/wash with TBS/Tween-20.7. Develop color using the enhanced DAB reaction. TOP Precautions All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines. Disclaimer While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information. TOP