View our list of available Coronavirus (COVID-19, SARS-CoV-2, 2019-nCOV) antibodies, recombinant proteins, ELISA Kits... Options Full Datasheet Printer Friendly Datasheet Prepare for Printing Full Datasheet Printer Friendly Datasheet Prepare for Printing Have Questions? Ask Us! Request A Quote Today! Add To Compare List Rabbit M-4 Muscarinic receptor IgG fraction Polyclonal Antibody Catalog # MBS352160 Unit / Price 0.1 mL / $735 +1 FREE 8GB USB 5x0.1 mL / $3,200 +4 FREE 8GB USB Product Name Product Info Accession #s Product Desc Diseases/Tissues/Pathways Applications References Product Name M-4 Muscarinic receptor IgG fraction, Polyclonal Antibody Full Product Name M-4 Muscarinic receptor IgG fraction Product Synonym Names Muscarinic M 4 Receptor; M 4 Receptor (469-478); IgG Fraction Rabbit Anti-Muscarinic M4 Receptor Serum Research Use Only For Research Use Only. Not for use in diagnostic procedures. TOP Clonality Polyclonal Host Rabbit Specificity Polypeptide ::: % Cross ReactivityM4 Muscarinic Receptor (469-478) ::: 100M4 Muscarinic Receptor ::: 70 Form/Format Antiserum Specificity Other Notes Small volumes of anti-M-4 Muscarinic receptor IgG fraction antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply. TOP Related Product Information for anti-M-4 Muscarinic receptor IgG fraction antibody This IgG fraction was prepared by precipitation. dialysis and chromatography from an antiserum that was raised in a rabbit which was immunized with a peptide analogue of the carboxyl terminal of the M4 receptor covalently attached onto a carrier protein. The antiserum has been shown to be specific for the COOH terminal of the M4 receptor and has been characterized by western blotting. ELISA. and cell staining techniques. This IgG fraction is suitable for immunocytochemical and western immunoblotting detection of the receptor. Dilute the lyophilized antibody with 0.1 ml of 10 mg/ml BSA in PBS for the equivalent concentration in whole antiserum. or with additional buffer for more dilute antibody. This vial contains 3.0 mg of purified IgG. Product Categories/Family for anti-M-4 Muscarinic receptor IgG fraction antibody Muscarinic Receptor Antibodies and Kits; Histochemical Grade Antibodies Applications Tested/Suitable for anti-M-4 Muscarinic receptor IgG fraction antibody Immunocytochemical Staining, Western Immunoblot, Western Blot Application Notes for anti-M-4 Muscarinic receptor IgG fraction antibody Immunocytochemistry: The purified IgG has been found to stain PLP fixed cultured cells known to express the muscarinic M4 receptor at a concentration of 50 ug/ml.Western Blot: Western immunoblots using whole rat brain homogenate and cultured cells known to express the M4 have been successful at a concentration of 80 ug/ml.Western Blot Protocol: 1. After SDS-PAGE (on either 4-15% gradient gels or single percentage gels. such at 12% gels) and electrophoretic transfer to PVDF membrane. block the membrane overnight with 2% normal goat serum in TBS/Tween-20 buffer.2. Wash x 2 with TBS/Tween-20.3. Apply the IgG fraction after diluting to at least 80 ug/ml (Note: higher dilutions may be needed). Use 2% normal goat serum in TBS/Tween-20 as buffer for the primary antibody. Let the primary antibody bind for 2-4 hours. Wash x 3 with TBS/Tween-20.4. Apply affinity purified HRP-goat anti-rabbit IgG antiserum diluted 1:2500 (dilution may vary depending upon supplier) in 2% normal goat serum in TBS/Tween-20. Incubate 1-2 hours. Note: greater sensitivity may be achieved using ABC techniques.5. Wash x 4 for 5 minute/wash with TBS/Tween-20.7. Develop color using the enhanced DAB reaction. TOP Precautions All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines. Disclaimer While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information. TOP