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anti-M-CSF antibody :: Rabbit M-CSF Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS551037 anti-M-CSF antibody
Unit / Price
0.1 mg  /  $280 +1 FREE 8GB USB
0.2 mg  /  $385 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

M-CSF, Polyclonal Antibody

★Popular Item★
 Also Known As   

Rabbit anti M-CSF Polyclonal

 Product Synonym Names    Rabbit anti M-CSF Polyclonal purified; CSF-1; csf1; m-csf; macrophage colony stimulting; mcsf; polyclonal; PAb
 Product Gene Name   

anti-M-CSF antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    120420
 3D Structure    ModBase 3D Structure for P09603
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 Clonality    Polyclonal
 Isotype    IgG
 Host    Rabbit
 Species Reactivity    Human, Mouse, Rat
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 Purity/Purification    >95%

Purification:Serum IgG fraction was purified by Protein G affinity chromatography.
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 Source Note    Polyclonal M-CSF antibody was produced from sera of rabbits immunized with synthetic protein containing M-CSF antigenic determinants.
 Reconstitution    A quick spin of the vial followed by reconstitution in distilled water. This solution can then be diluted into other buffers
 Presentation    0.2um filtered 1mg/ml solution in TBS pH 7.4, 50% glycerol, 1% BSA, 0.03% proclin
 Dilution    A quick spin of the vial followed by reconstitution in 0.01M PBS pH 7.4 or other diluents.
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 Preparation and Storage    The lyophilized antibody is stable for at least 1 year from date of receipt at -20 degree C.
Upon reconstitution, this antibody can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months without detectable loss of activity.

Avoid repeated freeze/thaw cycles.
Shelf Life: >12 months
 Other Notes    Small volumes of anti-M-CSF antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Searchable Terms for M-CSF purchase    As a proliferative protein, macrophage colony stimulating factor M CSF is commonly used in cell culture applications. In models, expression is tightly tied to CSF receptor expression. To accurately quantitate this human or mouse protein, MyBiosource catalog offers a top quality primary CSF antibody for use in human applications WB ( western blot ) or ELISA pairings. This human CSF antibody is ideal to study membrane receptor dynamics since binding is enhanced over competitors CSF antibodies and reactivity to common ALEXA fluor secondary antibodies is unparalleled. Our catalog is full of related reagents to complete your experiments, like mouse CSF primary antibodies, recombinant human CSF proteins, CSF antibody center reacting or CSF antibody internal reacting epitopes and phospho CSF receptor reagents. MBS551037 is in stock and available in two economical sizes at value prices. Order all your antibodies, protein loading positive controls and ELISA kits at MyBiosource.com in any size.
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Related Product Information for anti-M-CSF antibody

   M-CSF is produced by monocytes, granulocytes, endothelial cells, and fibroblasts. After cell activation, B-cells and T- cells and also a number of tumor cell lines are capable also of synthesizing this factor. M-CSF has been found to be synthesized by uterine epithelial cells in vivo. The M-CSF receptor is identical with the proto- oncogene fms. It has been renamed CD115. The receptor is a transmembrane protein with an extracellular ligand- binding domain of 512 amino acids, an intramembrane domain of 25 amino acids, and a cytoplasmic domain of 435 amino acids encoding a tyrosine kinase.Human M-CSF is active in mouse and rat cells. The murine factor is active in rat cells but inactive in human cells.M- CSF is a specific factor in that the proliferation inducing activity is more or less restricted to the macrophage lineage. M-CSF is a potent stimulator of functional activities of monocytes.
 Product Categories/Family for anti-M-CSF antibody    Polyclonal
 Applications Tested/Suitable for anti-M-CSF antibody   

Western Blot (WB), IHC (Immunohistochemistry), ELISA (EIA)

 Application Notes for anti-M-CSF antibody    ELISA: Use at a concentration range of 1-2ug/ml.
Western blot: A suitable range of concentrations of this antibody for WB detection is 2-10 ug/ml.
IHC: A suitable range of concentrations of this antibody for IHC is 2-10 ug/ml.
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NCBI/Uniprot data below describe general gene information for M-CSF. It may not necessarily be applicable to this product.
 NCBI GI #    27262661
 NCBI GeneID    1435
 NCBI Accession #    NP_000748.3 [Other Products]
 NCBI GenBank Nucleotide #    NM_000757.5 [Other Products]
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 UniProt Primary Accession #    P09603 [Other Products]
 UniProt Secondary Accession #    Q13130; Q14086; Q14806; Q5VVF3; Q5VVF4; Q9UQR8; A8K6J5 [Other Products]
 UniProt Related Accession #    P09603; Q5VVF2 [Other Products]
 Molecular Weight    60,179 Da
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 NCBI Official Full Name    macrophage colony-stimulating factor 1 isoform a
 UniProt Protein Name    Macrophage colony-stimulating factor 1
 UniProt Synonym Protein Names   
Lanimostim
 UniProt Gene Name    CSF1 [Similar Products]
 UniProt Synonym Gene Names    CSF-1; M-CSF; MCSF [Similar Products]
 UniProt Entry Name    CSF1_HUMAN
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 NCBI Summary for M-CSF    The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of macrophages. The active form of the protein is found extracellularly as a disulfide-linked homodimer, and is thought to be produced by proteolytic cleavage of membrane-bound precursors. The encoded protein may be involved in development of the placenta. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2011]
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 UniProt Comments for M-CSF    M-CSF: Cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. Required for normal male and female fertility. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration. Plays a role in lipoprotein clearance. Aberrant expression of CSF1 or CSF1R can promote cancer cell proliferation, invasion and formation of metastases. Overexpression of CSF1 or CSF1R is observed in a significant percentage of breast, ovarian, prostate, and endometrial cancers. Aberrant expression of CSF1 or CSF1R may play a role in inflammatory diseases, such as rheumatoid arthritis, glomerulonephritis, atherosclerosis, and allograft rejection. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytokine; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1p13.3

Cellular Component: extracellular space; membrane; perinuclear region of cytoplasm; plasma membrane; integral to membrane; receptor complex

Molecular Function: protein homodimerization activity; growth factor activity; cytokine activity; macrophage colony stimulating factor receptor binding

Biological Process: positive regulation of monocyte differentiation; ossification; macrophage differentiation; positive regulation of osteoclast differentiation; positive regulation of cellular protein metabolic process; positive regulation of odontogenesis of dentine-containing teeth; reproductive developmental process; osteoclast differentiation; positive regulation of multicellular organism growth; odontogenesis; cell proliferation; monocyte activation; positive regulation of mononuclear cell proliferation; homeostasis of number of cells within a tissue; positive regulation of protein kinase activity; positive regulation of cell proliferation; innate immune response; hemopoiesis; positive regulation of Ras protein signal transduction; positive regulation of cell-matrix adhesion; inflammatory response; positive regulation of macrophage differentiation; cell differentiation; regulation of ossification; positive regulation of cell migration
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Product References and Citations for anti-M-CSF antibody

   gM-CSF and il-4 induce dendritic cell differentiation and disrupt osteoclastogenesis through M-CSF receptor shedding by up-regulation of tnf- converting enzyme (tace) .Blood, Sep 2009; 10.1182/blood-2009-04-215020.
gM-CSF- and M-CSF-dependent macrophage phenotypes display differential dependence on type i interferon signaling .J. Leukoc. Biol., Aug 2009; 86: 411 - 421.
M-CSF inhibition selectively targets pathological angiogenesis and lymphangiogenesis .J. Exp. Med., May 2009; 206: 1089 - 1102.
c-reactive protein induces M-CSF release and macrophage proliferation .J. Leukoc. Biol., Feb 2009; 85: 262 - 267.
M-CSF preferentially induces c-fos via erk to specify monopoiesis whereas g-csf directs granulopoiesis via shp2 .Blood (ASH Annual Meeting Abstracts), Nov 2008; 112: 2887.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-M-CSF antibodyPathways associated with anti-M-CSF antibody
 Reference Product  PubMed Publications
 CSF1R antibody  >49 publications with M-CSF and CSF1R
 TNF antibody  >11 publications with M-CSF and TNF
 SMARCA4 antibody  >3 publications with M-CSF and SMARCA4
 Products by Pathway  Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokines And Inflammatory Response Pathway antibodies  Cytokines And Inflammatory Response Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 Hematopoietic Cell Lineage Pathway antibodies  Hematopoietic Cell Lineage Pathway Diagram
 Integrins In Angiogenesis Pathway antibodies  Integrins In Angiogenesis Pathway Diagram
 Osteoclast Differentiation Pathway antibodies  Osteoclast Differentiation Pathway Diagram
 Osteoclast Differentiation Pathway antibodies  Osteoclast Differentiation Pathway Diagram
 Rheumatoid Arthritis Pathway antibodies  Rheumatoid Arthritis Pathway Diagram
 Rheumatoid Arthritis Pathway antibodies  Rheumatoid Arthritis Pathway Diagram
Diseases associated with anti-M-CSF antibodyOrgans/Tissues associated with anti-M-CSF antibody
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >39 publications with M-CSF and Neoplasms
 Inflammation Antibodies  >20 publications with M-CSF and Inflammation
 Carcinoma Antibodies  >14 publications with M-CSF and Carcinoma
 Bone Diseases, Developmental Antibodies  >12 publications with M-CSF and Bone Diseases, Developmental
 Bone Resorption Antibodies  >10 publications with M-CSF and Bone Resorption
 Necrosis Antibodies  >10 publications with M-CSF and Necrosis
 Leukemia Antibodies  >9 publications with M-CSF and Leukemia
 Breast Neoplasms Antibodies  >8 publications with M-CSF and Breast Neoplasms
 Immune System Diseases Antibodies  >7 publications with M-CSF and Immune System Diseases
 Adenocarcinoma Antibodies  >7 publications with M-CSF and Adenocarcinoma
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >37 publications with M-CSF and Blood
 Liver Antibodies  >12 publications with M-CSF and Liver
 Lung Antibodies  >9 publications with M-CSF and Lung
 Brain Antibodies  >6 publications with M-CSF and Brain
 Connective Tissue Antibodies  >5 publications with M-CSF and Connective Tissue
 Kidney Antibodies  >5 publications with M-CSF and Kidney
 Mammary Gland Antibodies  >4 publications with M-CSF and Mammary Gland
 Testis Antibodies  >4 publications with M-CSF and Testis
 Uterus Antibodies  >4 publications with M-CSF and Uterus
 Intestine Antibodies  >4 publications with M-CSF and Intestine
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