Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource rightarrow Antibody rightarrow Mlkl rightarrow Polyclonal Mlkl  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Menu separator
separator
ELISA Kit ELISA Kit
CLIA Kit CLIA Kit
PCR Kit PCR Kit
Monoclonal Antibody Monoclonal Antibody
Polyclonal Antibody Polyclonal Antibody
Secondary Antibody Secondary Antibody
Antigen Antigen
Biochemical Biochemical
cDNA Clone cDNA Clone
siRNA siRNA
Peptide Peptide
Recombinant/Purified Protein Rec./Purified Protein

Custom ELISA Kit Custom ELISA Kit
Custom Protein Custom Protein
Custom Antibody Custom Antibody
Antibody Matched Pairs Antibody Matched Pairs
Antibody & Corresponding Blocking Peptide Pairs Antibody Peptide Pairs
Phospho-Specific Antibodies Phospho Antibodies
Products by Disease Products by Disease
Products by Pathway Products by Pathway
Products by Tissue Products by Tissue

arrow Advanced Search
arrow Submit Technical Q&A
arrow International Distributors
arrow Contact Us
separator
Our Best Sellers moreseparator
separator
 • 2-oxoglutarate dehydrogenase (OGDH) Antibody
 • Cytochrome P450, family 1, subfamily B, polypeptide 1 (CYP1B... ELISA Kit
 • Complement Component 3a (C3a) ELISA Kit
 • Long-chain fatty acid transport protein 1, SLC27A1 ELISA Kit
 • Polycystin-1 (PKD1) Recombinant Protein
 • S100 Calcium Binding Protein A9 (S100A9) ELISA Kit
 • cystathionine beta-synthase (CBS) ELISA Kit
 • Osteocalcin (BGLAP) ELISA Kit
 • Beta-Defensins 2 (DEFbeta2) ELISA Kit
 • S adenosylhomocysteine hydrolase (ACHY) (AHCY) Antibody
 • R-spondin 3 homolog (Xenopus laevis) (RSPO3) ELISA Kit
 • Caspase 3 (CASPASE-3) ELISA Kit
 • BMX Antibody
 • Vitamin B6 (VB6) ELISA Kit
 downarrow more ...
separator
separator
DatasheetFull DatasheetPrinter Friendly DatasheetPrint This DatasheetAdd to Compare ListHave Questions? Ask UsRequest for a Quotation today

anti-Mlkl antibody :: Rabbit anti-Mouse M Mlkl Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS9206965
Unit / Price
0.08 mL  /  $150 +1 FREE 8GB USB
0.4 mL  /  $340 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

M Mlkl, Polyclonal Antibody

 Also Known As   

M Mlkl Antibody (C-term)

 Product Synonym Names    Mixed lineage kinase domain-like protein; Mlkl {ECO:0000312|EMBL:AAH237551; ECO:0000312|MGI:MGI:1921818}
 Product Gene Name   

anti-Mlkl antibody

[Similar Products]
 Antibody/Peptide Pairs    M Mlkl peptide (MBS9220629) is used for blocking the activity of M Mlkl antibody (MBS9206965)
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
Table BarTOPTable Bar
 Immunogen Sequence Positions    444-472
 3D Structure    ModBase 3D Structure for Q9D2Y4
Section Bar
 Clonality    Polyclonal
 Isotype    Rabbit Ig
 Host    Rabbit
 Species Reactivity    Mouse
Section Bar
 Specificity    This Mouse Mlkl antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 444-472 amino acids from the C-terminal region of mouse Mlkl.
 Purity/Purification    Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
 Form/Format    Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
 Concentration    Vial Concentration: 0.5 (lot specific)
Section Bar
 Antigen Type    Synthetic Peptide
 Crown Antibody    Yes
 Antigen Source    MOUSE
Section Bar
 Preparation and Storage    Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
 Other Notes    Small volumes of anti-Mlkl antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Table BarTOPTable Bar
 

Related Product Information for anti-Mlkl antibody

   The protein kinase domain is predicted to be catalytically inactive. Molecular function: protein binding. There are two isoforms.
 Product Categories/Family for anti-Mlkl antibody    Crown Antibodies; Signal Transduction
 Applications Tested/Suitable for anti-Mlkl antibody   

Western Blot (WB), ELISA (EIA)

 Application Notes for anti-Mlkl antibody    WB~~1:1000
Section Bar
 Western Blot (WB) of anti-Mlkl antibody    All lanes : Anti-Mlkl Antibody (C-term) at 1:2000 dilution
Lane 1: mouse testis lysates
Lane 2: mouse liver lysates
Lane 3: NIH/3T3 whole cell lysates

Lysates/proteins at 20 ug per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution

Predicted band size : 54 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
anti-Mlkl antibody Western Blot (WB) (WB) image
Section Bar
 Western Blot (WB) of anti-Mlkl antibody    All lanes : Anti-Mlkl Antibody (C-term) at 1:2000 dilution
Lane 1: NIH/3T3 whole cell lysates
Lane 2: mouse spleen lysates

Lysates/proteins at 20 ug per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution

Predicted band size : 54 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
anti-Mlkl antibody Western Blot (WB) (WB) image
Section Bar
 Western Blot (WB) of anti-Mlkl antibody    Anti-Mlkl Antibody (C-term)at 1:1000 dilution + mouse lung lysates

Lysates/proteins at 20 ug per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution

Predicted band size : 54 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
anti-Mlkl antibody Western Blot (WB) (WB) image
Section Bar
 Western Blot (WB) of anti-Mlkl antibody    Anti-Mlkl Antibody (C-term)at 1:2000 dilution + NIH/3T3 whole cell lysates

Lysates/proteins at 20 ug per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution

Predicted band size : 54 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
anti-Mlkl antibody Western Blot (WB) (WB) image
Section Bar
 Western Blot (WB) of anti-Mlkl antibody    All lanes : Anti-Mlkl Antibody (C-term) at 1:1000 dilution
Lane 1: mouse lung lysates
Lane 2: mouse spleen lysates

Lysates/proteins at 20 ug per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution

Predicted band size : 54 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
anti-Mlkl antibody Western Blot (WB) (WB) image
Section Bar
 Western Blot (WB) of anti-Mlkl antibody    All lanes : Anti-Mlkl Antibody (C-term) at 1:2000 dilution
Lane 1: mouse lung lysates
Lane 2: NIH/3T3 whole cell lysates
Lane 3: mouse liver lysates
Lane 4: mouse testis whole cell lysates

Lysates/proteins at 20 ug per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution

Predicted band size : 54 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
anti-Mlkl antibody Western Blot (WB) (WB) image
Section Bar
 Western Blot (WB) of anti-Mlkl antibody    Western blot analysis of lysate from mouse lung tissue lysate, using Mlkl Antibody (C-term). MBS9206965 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.
anti-Mlkl antibody Western Blot (WB) (WB) image
Table BarTOPTable Bar
NCBI/Uniprot data below describe general gene information for Mlkl. It may not necessarily be applicable to this product.
 NCBI GI #    110626037
 NCBI GeneID    74568
 NCBI Accession #    NP_083281.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_029005.2 [Other Products]
Section Bar
 UniProt Primary Accession #    Q9D2Y4 [Other Products]
 UniProt Secondary Accession #    Q8CIJ5 [Other Products]
 UniProt Related Accession #    Q9D2Y4 [Other Products]
 Molecular Weight    54317
Table BarTOPTable Bar
 NCBI Official Full Name    mixed lineage kinase domain-like protein
 NCBI Official Synonym Full Names    mixed lineage kinase domain-like
 NCBI Official Symbol    Mlkl [Similar Products]
 NCBI Official Synonym Symbols   
9130019I15Rik
[Similar Products]
 NCBI Protein Information    mixed lineage kinase domain-like protein
 UniProt Protein Name    Mixed lineage kinase domain-like protein
 Protein Family    Mixed lineage kinase domain-like protein
 UniProt Gene Name    Mlkl [Similar Products]
 UniProt Entry Name    MLKL_MOUSE
Section Bar
 NCBI Summary for Mlkl    This gene belongs to the protein kinase superfamily. The encoded protein contains a protein kinase-like domain; however, is thought to lack protein kinase activity. This protein plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (Rip3), which is a key signaling molecule in necroptosis pathway. Knockout of this gene in mice showed that it is essential for necroptosis. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2015]
Section Bar
 UniProt Comments for Mlkl    MLKL: Belongs to the protein kinase superfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Protein kinase, TKL; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; TKL group; TKL-Unique family

Cellular Component: membrane; cytoplasm; plasma membrane

Molecular Function: transferase activity, transferring phosphorus-containing groups; nucleotide binding; protein complex binding; protein kinase binding; ATP binding; protein kinase activity

Biological Process: programmed cell death; protein amino acid phosphorylation
Table BarTOPTable Bar
 

Product References and Citations for anti-Mlkl antibody

   Bisson, N., et al. Cell Cycle 7(7):909-916(2008)
Section Bar
 Research Articles on Mlkl    1. MLKL acctivation unleashes the four-helix bundle domain to induce membrane localization and necroptotic cell death
Table BarTOPTable Bar
 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Table BarTOPTable Bar
Products associated with anti-Mlkl antibodyPathways associated with anti-Mlkl antibody
 Reference Product  PubMed Publications
 Ripk3 antibody  >68 publications with Mlkl and Ripk3
 Pgam5 antibody  >9 publications with Mlkl and Pgam5
 Products by Pathway  Pathway Diagram
 , Pathway antibodies  , Pathway Diagram
 , Pathway antibodies  , Pathway Diagram
 , Pathway antibodies  , Pathway Diagram
 , Pathway antibodies  , Pathway Diagram
 , Pathway antibodies  , Pathway Diagram
Diseases associated with anti-Mlkl antibodyOrgans/Tissues associated with anti-Mlkl antibody
 Disease Name  Pubmed Publications
 Necrosis Antibodies  >87 publications with Mlkl and Necrosis
 Inflammation Antibodies  >34 publications with Mlkl and Inflammation
 Liver Diseases Antibodies  >7 publications with Mlkl and Liver Diseases
 Kidney Diseases Antibodies  >5 publications with Mlkl and Kidney Diseases
 Disease Models, Animal Antibodies  >4 publications with Mlkl and Disease Models, Animal
 Drug-Induced Liver Injury Antibodies  >4 publications with Mlkl and Drug-Induced Liver Injury
 Fibrosis Antibodies  >2 publications with Mlkl and Fibrosis
 Drug-Related Side Effects and Adverse Reactions Antibodies  >2 publications with Mlkl and Drug-Related Side Effects and Adverse Reactions
 Poisoning Antibodies  >2 publications with Mlkl and Poisoning
 Lymphoma Antibodies  >2 publications with Mlkl and Lymphoma
 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >10 publications with Mlkl and Liver
 Kidney Antibodies  >5 publications with Mlkl and Kidney
 Lung Antibodies  >3 publications with Mlkl and Lung
 Intestine Antibodies  >3 publications with Mlkl and Intestine
 Embryonic Tissue Antibodies  >2 publications with Mlkl and Embryonic Tissue
 Skin Antibodies  >1 publications with Mlkl and Skin
 Uncharacterized Tissue Antibodies  >1 publications with Mlkl and Uncharacterized Tissue
 Bone Marrow Antibodies  >1 publications with Mlkl and Bone Marrow
Table BarTOPTable Bar
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions