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anti-MAD2L2 antibody :: Rabbit anti-Human MAD2B / REV7 Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS240549
Unit / Price
0.05 mg  /  $495 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

MAD2B / REV7 (MAD2L2), Polyclonal Antibody

 Also Known As   

Rabbit Polyclonal to Human MAD2B / REV7

 Product Synonym Names    Anti-MAD2B / REV7 Antibody (aa3-14) IHC-plus; POLZ2; REV7 homolog; MAD2-like protein 2; HREV7; Human MAD2B; REV7
 Product Gene Name   

anti-MAD2L2 antibody

[Similar Products]
 Product Synonym Gene Name    MAD2B; REV7 [Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    604094
 3D Structure    ModBase 3D Structure for Q9UI95
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 Clonality    Polyclonal
 Host    Rabbit
 Species Reactivity    Human
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 Specificity    Aa 3-14 of Human MAD2L2. MAD2L2 is also known as MAD2B, REV7 and mitotic arrest deficient-like 2 protein.
 Purity/Purification    Immunoaffinity Purified
 Form/Format    0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
 Concentration    1 mg/ml (lot specific)
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 Target Species    Human
 Immunogen Description    Synthetic peptide from human MAD2L2.
 Immunogen Type    Synthetic peptide
 Immunogen    MAD2B / REV7 antibody was raised against synthetic peptide from human MAD2L2.
 Antigen Modification    aa3-14
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 Preparation and Storage    Long term: -20 degree C; Short term: +4 degree C. Avoid repeat freeze-thaw cycles.
 Other Notes    Small volumes of anti-MAD2L2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Applications Tested/Suitable for anti-MAD2L2 antibody   

Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)

 Application Notes for anti-MAD2L2 antibody    ELISA (1:7500 - 1:30000), IF, IHC-P (10 ug/ml), WB (1:500 - 1:2000)
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antib...
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 Immunohistochemistry (IHC) of anti-MAD2L2 antibody    Anti-MAD2L2 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
anti-MAD2L2 antibody Immunohistochemistry (IHC) (IHC) image
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 Western Blot (WB) of anti-MAD2L2 antibody    Anti-MAD2L2 Antibody - Western Blot. Affinity Purified Rabbit anti-MAD2L2 was used at a 1:500 dilution to detect human MAD2L2 by western blot. Both HeLa whole cell lysate (lane 1) and nuclear lysate (lane 2) were probed using this antibody. This antibody clearly detects a ~20 kD band corresponding to human MAD2L2 (predicted molecular weight is 24 kD). Approximately 20 ug of each lysate was loaded on a 10% SDS-PAGE. Primary antibody was reacted with the membrane at room temperature for 1 h. After subsequent washing, a 1:2000 dilution of HRP conjugated Gt-a-Rabbit IgG was used for visualization. Exposure time was 30 sec.
anti-MAD2L2 antibody Western Blot (WB) (WB) image
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NCBI/Uniprot data below describe general gene information for MAD2L2. It may not necessarily be applicable to this product.
 NCBI GI #    187960073
 NCBI GeneID    10459
 NCBI Accession #    NP_006332.3 [Other Products]
 NCBI GenBank Nucleotide #    NM_006341.3 [Other Products]
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 UniProt Primary Accession #    Q9UI95 [Other Products]
 UniProt Secondary Accession #    Q5TGW7; Q9UNA7; Q9Y6I6; B3KNE3 [Other Products]
 UniProt Related Accession #    Q9UI95 [Other Products]
 Molecular Weight    24,334 Da
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 NCBI Official Full Name    mitotic spindle assembly checkpoint protein MAD2B
 NCBI Official Synonym Full Names    MAD2 mitotic arrest deficient-like 2 (yeast)
 NCBI Official Symbol    MAD2L2 [Similar Products]
 NCBI Official Synonym Symbols   
REV7; MAD2B; POLZ2
[Similar Products]
 NCBI Protein Information    mitotic spindle assembly checkpoint protein MAD2B; hREV7; REV7 homolog; MAD2-like protein 2; mitotic arrest deficient homolog-like 2; mitotic arrest deficient 2-like protein 2; polymerase (DNA-directed), zeta 2, accessory subunit; MAD2 (mitotic arrest deficient, yeast, homolog)-like 2
 UniProt Protein Name    Mitotic spindle assembly checkpoint protein MAD2B
 UniProt Synonym Protein Names   
Mitotic arrest deficient 2-like protein 2; MAD2-like protein 2; REV7 homolog; hREV7
 Protein Family    Mitotic spindle assembly checkpoint protein
 UniProt Gene Name    MAD2L2 [Similar Products]
 UniProt Synonym Gene Names    MAD2B; REV7; MAD2-like protein 2; hREV7 [Similar Products]
 UniProt Entry Name    MD2L2_HUMAN
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 NCBI Summary for MAD2L2    The protein encoded by this gene is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. The encoded protein, which is similar to MAD2L1, is capable of interacting with ADAM9, ADAM15, REV1, and REV3 proteins. [provided by RefSeq, Jul 2008]
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 UniProt Comments for MAD2L2    MAD2L2: Adapter protein able to interact with different proteins and involved in different biological processes. Mediates the interaction between the error-prone DNA polymerase zeta catalytic subunit REV3L and the inserter polymerase REV1, thereby mediating the second polymerase switching in translesion DNA synthesis. Translesion DNA synthesis releases the replication blockade of replicative polymerases, stalled in presence of DNA lesions. May also regulate another aspect of cellular response to DNA damage through regulation of the JNK-mediated phosphorylation and activation of the transcriptional activator ELK1. Inhibits the FZR1- and probably CDC20-mediated activation of the anaphase promoting complex APC thereby regulating progression through the cell cycle. Regulates TCF7L2-mediated gene transcription and may play a role in epithelial-mesenchymal transdifferentiation.

Chromosomal Location of Human Ortholog: 1p36

Cellular Component: nucleoplasm; anaphase-promoting complex; zeta DNA polymerase complex; cytoplasm; spindle; nucleus

Molecular Function: JUN kinase binding; protein binding

Biological Process: mitosis; transcription, DNA-dependent; negative regulation of transcription factor activity; positive regulation of transcription, DNA-dependent; actin filament organization; negative regulation of transcription from RNA polymerase II promoter; DNA repair; positive regulation of peptidyl-serine phosphorylation; cell division; double-strand break repair; mitotic cell cycle spindle assembly checkpoint; DNA damage response, signal transduction resulting in transcription; regulation of cell growth; negative regulation of protein catabolic process
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 Research Articles on MAD2L2    1. These findings indicate that depletion of REV7 enhances sensitivity to cisplatin treatment in ovarian clear cell carcinoma (CCC), suggesting that REV7 is a candidate molecular target in CCC management.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-MAD2L2 antibodyPathways associated with anti-MAD2L2 antibody
 Reference Product  PubMed Publications
 CDC20 antibody  >18 publications with MAD2L2 and CDC20
 REV3L antibody  >17 publications with MAD2L2 and REV3L
 REV1 antibody  >15 publications with MAD2L2 and REV1
 BUB1 antibody  >12 publications with MAD2L2 and BUB1
 PRCC antibody  >4 publications with MAD2L2 and PRCC
 FZR1 antibody  >2 publications with MAD2L2 and FZR1
 ADAM9 antibody  >1 publications with MAD2L2 and ADAM9
 Products by Pathway  Pathway Diagram
 Bacterial Invasion Of Epithelial Cells Pathway antibodies  Bacterial Invasion Of Epithelial Cells Pathway Diagram
 Bacterial Invasion Of Epithelial Cells Pathway antibodies  Bacterial Invasion Of Epithelial Cells Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 DNA Damage Bypass Pathway antibodies  DNA Damage Bypass Pathway Diagram
 DNA Repair Pathway antibodies  DNA Repair Pathway Diagram
 DNA Polymerase Zeta Complex Pathway antibodies  DNA Polymerase Zeta Complex Pathway Diagram
 DNA Polymerase Zeta Complex Pathway antibodies  DNA Polymerase Zeta Complex Pathway Diagram
 Oocyte Meiosis Pathway antibodies  Oocyte Meiosis Pathway Diagram
Diseases associated with anti-MAD2L2 antibodyOrgans/Tissues associated with anti-MAD2L2 antibody
 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >14 publications with MAD2L2 and Carcinoma
 Adenocarcinoma Antibodies  >6 publications with MAD2L2 and Adenocarcinoma
 Kidney Diseases Antibodies  >4 publications with MAD2L2 and Kidney Diseases
 Brain Diseases Antibodies  >2 publications with MAD2L2 and Brain Diseases
 Carcinoma, Hepatocellular Antibodies  >2 publications with MAD2L2 and Carcinoma, Hepatocellular
 Colonic Neoplasms Antibodies  >2 publications with MAD2L2 and Colonic Neoplasms
 Anemia Antibodies  >2 publications with MAD2L2 and Anemia
 Drug-Induced Liver Injury Antibodies  >1 publications with MAD2L2 and Drug-Induced Liver Injury
 Drug-Related Side Effects and Adverse Reactions Antibodies  >1 publications with MAD2L2 and Drug-Related Side Effects and Adverse Reactions
 Poisoning Antibodies  >1 publications with MAD2L2 and Poisoning
 Organ/Tissue Name  Pubmed Publications
 Kidney Antibodies  >4 publications with MAD2L2 and Kidney
 Liver Antibodies  >4 publications with MAD2L2 and Liver
 Brain Antibodies  >3 publications with MAD2L2 and Brain
 Testis Antibodies  >2 publications with MAD2L2 and Testis
 Lymph Antibodies  >2 publications with MAD2L2 and Lymph
 Lymph Node Antibodies  >2 publications with MAD2L2 and Lymph Node
 Intestine Antibodies  >2 publications with MAD2L2 and Intestine
 Embryonic Tissue Antibodies  >2 publications with MAD2L2 and Embryonic Tissue
 Prostate Antibodies  >1 publications with MAD2L2 and Prostate
 Lung Antibodies  >1 publications with MAD2L2 and Lung
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