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| Product Name
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MAD2B / REV7 (MAD2L2), Polyclonal Antibody
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| Also Known As
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Rabbit Polyclonal to Human MAD2B / REV7
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| Product Synonym Names
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Anti-MAD2B / REV7 Antibody (aa3-14) IHC-plus; POLZ2; REV7 homolog; MAD2-like protein 2; HREV7; Human MAD2B; REV7
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| Product Gene Name
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[Similar Products]
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| Product Synonym Gene Name
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MAD2B; REV7
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[Similar Products]
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| Research Use Only
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For Research Use Only. Not for use in diagnostic procedures.
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 TOP
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| OMIM
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604094
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| 3D Structure
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ModBase 3D Structure for Q9UI95
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| Clonality
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Polyclonal
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| Host
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Rabbit
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| Species Reactivity
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Human
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| Specificity
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Aa 3-14 of Human MAD2L2. MAD2L2 is also known as MAD2B, REV7 and mitotic arrest deficient-like 2 protein.
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| Purity/Purification
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Immunoaffinity Purified
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| Form/Format
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0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
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| Concentration
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1 mg/ml (lot specific)
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| Target Species
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Human
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| Immunogen Description
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Synthetic peptide from human MAD2L2.
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| Immunogen Type
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Synthetic peptide
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| Immunogen
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MAD2B / REV7 antibody was raised against synthetic peptide from human MAD2L2.
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| Antigen Modification
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aa3-14
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| Preparation and Storage
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Long term: -20 degree C; Short term: +4 degree C. Avoid repeat freeze-thaw cycles.
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| Other Notes
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Small volumes of anti-MAD2L2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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TOP
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| Applications Tested/Suitable for anti-MAD2L2 antibody
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Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
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| Application Notes for anti-MAD2L2 antibody
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ELISA (1:7500 - 1:30000), IF, IHC-P (10 ug/ml), WB (1:500 - 1:2000)
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antib...
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| Immunohistochemistry (IHC) of anti-MAD2L2 antibody
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Anti-MAD2L2 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
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| Western Blot (WB) of anti-MAD2L2 antibody
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Anti-MAD2L2 Antibody - Western Blot. Affinity Purified Rabbit anti-MAD2L2 was used at a 1:500 dilution to detect human MAD2L2 by western blot. Both HeLa whole cell lysate (lane 1) and nuclear lysate (lane 2) were probed using this antibody. This antibody clearly detects a ~20 kD band corresponding to human MAD2L2 (predicted molecular weight is 24 kD). Approximately 20 ug of each lysate was loaded on a 10% SDS-PAGE. Primary antibody was reacted with the membrane at room temperature for 1 h. After subsequent washing, a 1:2000 dilution of HRP conjugated Gt-a-Rabbit IgG was used for visualization. Exposure time was 30 sec.
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TOP
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| NCBI/Uniprot data below describe general gene information for MAD2L2. It may not necessarily be applicable to this product. |
| NCBI GI #
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187960073
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| NCBI GeneID
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10459
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| NCBI Accession #
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NP_006332.3
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[Other Products]
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| NCBI GenBank Nucleotide #
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NM_006341.3
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[Other Products]
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| UniProt Primary Accession #
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Q9UI95
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[Other Products]
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| UniProt Secondary Accession #
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Q5TGW7; Q9UNA7; Q9Y6I6; B3KNE3
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[Other Products]
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| UniProt Related Accession #
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Q9UI95
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[Other Products]
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| Molecular Weight
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24,334 Da
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TOP
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| NCBI Official Full Name
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mitotic spindle assembly checkpoint protein MAD2B
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| NCBI Official Synonym Full Names
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MAD2 mitotic arrest deficient-like 2 (yeast)
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| NCBI Official Symbol
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MAD2L2
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[Similar Products]
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| NCBI Official Synonym Symbols
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REV7; MAD2B; POLZ2
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[Similar Products]
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| NCBI Protein Information
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mitotic spindle assembly checkpoint protein MAD2B; hREV7; REV7 homolog; MAD2-like protein 2; mitotic arrest deficient homolog-like 2; mitotic arrest deficient 2-like protein 2; polymerase (DNA-directed), zeta 2, accessory subunit; MAD2 (mitotic arrest deficient, yeast, homolog)-like 2
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| UniProt Protein Name
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Mitotic spindle assembly checkpoint protein MAD2B
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| UniProt Synonym Protein Names
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Mitotic arrest deficient 2-like protein 2; MAD2-like protein 2; REV7 homolog; hREV7
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| Protein Family
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Mitotic spindle assembly checkpoint protein
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| UniProt Gene Name
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MAD2L2
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[Similar Products]
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| UniProt Synonym Gene Names
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MAD2B; REV7; MAD2-like protein 2; hREV7
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[Similar Products]
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| UniProt Entry Name
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MD2L2_HUMAN
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| NCBI Summary for MAD2L2
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The protein encoded by this gene is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. The encoded protein, which is similar to MAD2L1, is capable of interacting with ADAM9, ADAM15, REV1, and REV3 proteins. [provided by RefSeq, Jul 2008]
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| UniProt Comments for MAD2L2
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MAD2L2: Adapter protein able to interact with different proteins and involved in different biological processes. Mediates the interaction between the error-prone DNA polymerase zeta catalytic subunit REV3L and the inserter polymerase REV1, thereby mediating the second polymerase switching in translesion DNA synthesis. Translesion DNA synthesis releases the replication blockade of replicative polymerases, stalled in presence of DNA lesions. May also regulate another aspect of cellular response to DNA damage through regulation of the JNK-mediated phosphorylation and activation of the transcriptional activator ELK1. Inhibits the FZR1- and probably CDC20-mediated activation of the anaphase promoting complex APC thereby regulating progression through the cell cycle. Regulates TCF7L2-mediated gene transcription and may play a role in epithelial-mesenchymal transdifferentiation.
Chromosomal Location of Human Ortholog: 1p36
Cellular Component: nucleoplasm; anaphase-promoting complex; zeta DNA polymerase complex; cytoplasm; spindle; nucleus
Molecular Function: JUN kinase binding; protein binding
Biological Process: mitosis; transcription, DNA-dependent; negative regulation of transcription factor activity; positive regulation of transcription, DNA-dependent; actin filament organization; negative regulation of transcription from RNA polymerase II promoter; DNA repair; positive regulation of peptidyl-serine phosphorylation; cell division; double-strand break repair; mitotic cell cycle spindle assembly checkpoint; DNA damage response, signal transduction resulting in transcription; regulation of cell growth; negative regulation of protein catabolic process
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TOP
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| Research Articles on MAD2L2
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1. These findings indicate that depletion of REV7 enhances sensitivity to cisplatin treatment in ovarian clear cell carcinoma (CCC), suggesting that REV7 is a candidate molecular target in CCC management.
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TOP
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| Precautions
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All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
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| Disclaimer
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While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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| Products associated with anti-MAD2L2 antibody | Pathways associated with anti-MAD2L2 antibody |
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| Diseases associated with anti-MAD2L2 antibody | Organs/Tissues associated with anti-MAD2L2 antibody |
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Antibody 
