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anti-MAPK1 antibody :: Rabbit anti-Human, Mouse MAPK1 Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS7045563
Unit / Price
0.05 mg  /  $160 +1 FREE 8GB USB
0.1 mg  /  $235 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

MAPK1, Polyclonal Antibody

★Popular Item★
 Also Known As   

MAPK1 Antibody

 Product Synonym Names    Mitogen-activated protein kinase 1; ERT1; Extracellular signal-regulated kinase 2; ERK-2; MAP kinase isoform p42; p42-MAPK; Mitogen-activated protein kinase; MAPK1; ERK2; PRKM1; PRKM2
 Product Gene Name   

anti-MAPK1 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    176948
 3D Structure    ModBase 3D Structure for P28482
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 Clonality    Polyclonal
 Isotype    IgG
 Host    Rabbit
 Species Reactivity    Human, Mouse
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 Purity/Purification    >95%, Protein G purified
 Form/Format    Liquid
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 Species    Human
 Immunogen    Recombinant human Mitogen-activated protein kinase 1 protein
 Conjugate    Non-conjugated
 Buffer    Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
 Santa Cruz Alternative    Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-154 / sc-153
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 Preparation and Storage    Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Other Notes    Small volumes of anti-MAPK1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-MAPK1 antibody

   Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. About 160 substrates have already been discovered for ERKs. Many of these substrates are localized in the nucleus, and seem to participate in the regulation of transcription upon stimulation. However, other substrates are found in the cytosol as well as in other cellular organelles, and those are responsible for processes such as translation, mitosis and apoptosis. Moreover, the MAPK/ERK cascade is also involved in the regulation of the endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC); as well as in the fragmentation of the Golgi apparatus during mitosis. The substrates include transcription factors (such as ATF2, BCL6, ELK1, ERF, FOS, HSF4 or SPZ1), cytoskeletal elements (such as CANX, CTTN, GJA1, MAP2, MAPT, PXN, SORBS3 or STMN1), regulators of apoptosis (such as BAD, BTG2, CASP9, DAPK1, IER3, MCL1 or PPARG), regulators of translation (such as EIF4EBP1) and a variety of other signaling-related molecules (like ARHGEF2, DCC, FRS2 or GRB10). Protein kinases (such as RAF1, RPS6KA1/RSK1, RPS6KA3/RSK2, RPS6KA2/RSK3, RPS6KA6/RSK4, SYK, MKNK1/MNK1, MKNK2/MNK2, RPS6KA5/MSK1, RPS6KA4/MSK2, MAPKAPK3 or MAPKAPK5) and phosphatases (such as DUSP1, DUSP4, DUSP6 or DUSP16) are other substrates which enable the propagation the MAPK/ERK signal to additional cytosolic and nuclear targets, thereby extending the specificity of the cascade. Mediates phosphorylation of TPR in respons to EGF stimulation. May play a role in the spindle assembly checkpoint. Phosphorylates PML and promotes its interaction with PIN1, leading to PML degradation.
 Applications Tested/Suitable for anti-MAPK1 antibody   

ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

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 Western Blot (WB) of anti-MAPK1 antibody    Western Blot
Positive WB detected in: Hela whole cell lysate, Jurkat whole cell lysate, A549 whole cell lysate,293 whole cell lysate,Rat lung tissue
All lanes: MAPK1 antibody at 4.8ug/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 42,37 KDa
Observed band size: 42,37 KDa

anti-MAPK1 antibody Western Blot (WB) (WB) image
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 Immunohistochemistry (IHC) of anti-MAPK1 antibody    IHC image of MBS7045563 diluted at 1:600 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
anti-MAPK1 antibody Immunohistochemistry (IHC) (IHC) image
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 Immunohistochemistry (IHC) of anti-MAPK1 antibody    IHC image of MBS7045563 diluted at 1:600 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
anti-MAPK1 antibody Immunohistochemistry (IHC) (IHC) image
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 Immunofluorescence (IF) of anti-MAPK1 antibody    Immunofluorescent analysis of HepG-2 cells using MBS7045563 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
anti-MAPK1 antibody Immunofluorescence (IF) image
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NCBI/Uniprot data below describe general gene information for MAPK1. It may not necessarily be applicable to this product.
 NCBI GI #    66932916
 NCBI GeneID    5594
 NCBI Accession #    NP_002736.3 [Other Products]
 NCBI GenBank Nucleotide #    NM_002745.4 [Other Products]
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 UniProt Primary Accession #    P28482 [Other Products]
 UniProt Secondary Accession #    A8CZ64 [Other Products]
 UniProt Related Accession #    P28482 [Other Products]
 Molecular Weight    36,432 Da
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 NCBI Official Full Name    mitogen-activated protein kinase 1
 NCBI Official Synonym Full Names    mitogen-activated protein kinase 1
 NCBI Official Symbol    MAPK1 [Similar Products]
 NCBI Official Synonym Symbols   
ERK; p38; p40; p41; ERK2; ERT1; ERK-2; MAPK2; PRKM1; PRKM2; P42MAPK; p41mapk; p42-MAPK
[Similar Products]
 NCBI Protein Information    mitogen-activated protein kinase 1
 UniProt Protein Name    Mitogen-activated protein kinase 1
 UniProt Synonym Protein Names   
ERT1; Extracellular signal-regulated kinase 2; ERK-2; MAP kinase isoform p42; p42-MAPK; Mitogen-activated protein kinase 2; MAP kinase 2; MAPK 2
 UniProt Gene Name    MAPK1 [Similar Products]
 UniProt Synonym Gene Names    ERK2; PRKM1; PRKM2; MAP kinase 1; MAPK 1; ERK-2; p42-MAPK; MAP kinase 2; MAPK 2 [Similar Products]
 UniProt Entry Name    MK01_HUMAN
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 NCBI Summary for MAPK1    This gene encodes a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. One study also suggests that this protein acts as a transcriptional repressor independent of its kinase activity. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene. [provided by RefSeq, Jan 2014]
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 UniProt Comments for MAPK1    ERK2: a serine/threonine kinase of the GMGC group that plays a critical role in the regulation of cell growth and differentiation. ERK1 (MAPK3) and ERK2 (MAPK1) play central roles in MAPK cascades and are activated by a wide variety of extracellular signals including growth and neurotrophic factors, cytokines, hormones and neurotransmitters. Depending on the cellular context, MAPK cascades mediate diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. MAPK cascades also plays a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. Activation of MAP kinases occurs through phosphorylation of threonine and tyrosine residues at the sequence T*EY* by upstream MAP kinase kinases, MEK1 and -2. Phosphorylation of both the threonine and tyrosine are required for activity. This phosphorylation causes dramatic conformational changes, which enable full activation and interaction of MAPK1/ERK2 with its substrates.

Protein type: EC 2.7.11.24; Kinase, protein; Protein kinase, CMGC; Protein kinase, Ser/Thr (non-receptor); CMGC group; MAPK family; ERK subfamily; MAPK/ERK subfamily

Chromosomal Location of Human Ortholog: 22q11.21

Cellular Component: caveola; cytoplasm; cytoskeleton; cytosol; early endosome; focal adhesion; Golgi apparatus; late endosome; microtubule cytoskeleton; mitochondrion; nucleoplasm; nucleus

Molecular Function: identical protein binding; kinase activity; MAP kinase activity; phosphatase binding; protein binding; protein serine/threonine kinase activity; RNA polymerase subunit kinase activity

Biological Process: activation of MAPK activity; axon guidance; chemotaxis; fibroblast growth factor receptor signaling pathway; MAPKKK cascade; peptidyl-serine phosphorylation; peptidyl-threonine phosphorylation; platelet activation; positive regulation of telomerase activity; positive regulation of telomere maintenance via telomerase; protein amino acid phosphorylation; regulation of cytoskeleton organization and biogenesis; regulation of phosphoinositide 3-kinase cascade; regulation of protein stability; regulation of stress-activated MAPK cascade; regulation of transcription factor activity; response to stress; signal transduction; synaptic transmission
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 Research Articles on MAPK1    1. The Asian-American variant of human papillomavirus type 16 exhibits higher activation of MAPK and PI3K/AKT signaling pathways, transformation, migration and invasion of primary human keratinocytes.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-MAPK1 antibodyPathways associated with anti-MAPK1 antibody
 Reference Product  PubMed Publications
 JUN antibody  >2099 publications with MAPK1 and JUN
 MAP2K1 antibody  >415 publications with MAPK1 and MAP2K1
 DUSP1 antibody  >123 publications with MAPK1 and DUSP1
 RAF1 antibody  >68 publications with MAPK1 and RAF1
 MBP antibody  >56 publications with MAPK1 and MBP
 RPS6KA1 antibody  >34 publications with MAPK1 and RPS6KA1
 STAT5A antibody  >18 publications with MAPK1 and STAT5A
 PTPN7 antibody  >10 publications with MAPK1 and PTPN7
 PTPRR antibody  >6 publications with MAPK1 and PTPRR
 RPS6KA3 antibody  >2 publications with MAPK1 and RPS6KA3
 Products by Pathway  Pathway Diagram
 AGE-RAGE Signaling Pathway In Diabetic Complications antibodies  AGE-RAGE Signaling Pathway In Diabetic Complications Diagram
 AGE-RAGE Signaling Pathway In Diabetic Complications antibodies  AGE-RAGE Signaling Pathway In Diabetic Complications Diagram
 AGE/RAGE Pathway antibodies  AGE/RAGE Pathway Diagram
 ALK1 Signaling Events Pathway antibodies  ALK1 Signaling Events Pathway Diagram
 ARMS-mediated Activation Pathway antibodies  ARMS-mediated Activation Pathway Diagram
 ATF-2 Transcription Factor Network Pathway antibodies  ATF-2 Transcription Factor Network Pathway Diagram
 Activated TLR4 Signalling Pathway antibodies  Activated TLR4 Signalling Pathway Diagram
 Activation Of NMDA Receptor Upon Glutamate Binding And Postsynaptic Events Pathway antibodies  Activation Of NMDA Receptor Upon Glutamate Binding And Postsynaptic Events Pathway Diagram
 Activation Of The AP-1 Family Of Transcription Factors Pathway antibodies  Activation Of The AP-1 Family Of Transcription Factors Pathway Diagram
 Acute Myeloid Leukemia Pathway antibodies  Acute Myeloid Leukemia Pathway Diagram
Diseases associated with anti-MAPK1 antibodyOrgans/Tissues associated with anti-MAPK1 antibody
 Disease Name  Pubmed Publications
 Necrosis Antibodies  >833 publications with MAPK1 and Necrosis
 Inflammation Antibodies  >810 publications with MAPK1 and Inflammation
 Breast Neoplasms Antibodies  >435 publications with MAPK1 and Breast Neoplasms
 Heart Diseases Antibodies  >347 publications with MAPK1 and Heart Diseases
 Hypertension Antibodies  >271 publications with MAPK1 and Hypertension
 Hypertrophy Antibodies  >267 publications with MAPK1 and Hypertrophy
 Liver Diseases Antibodies  >253 publications with MAPK1 and Liver Diseases
 Neoplasms, Experimental Antibodies  >243 publications with MAPK1 and Neoplasms, Experimental
 Lung Neoplasms Antibodies  >233 publications with MAPK1 and Lung Neoplasms
 Neoplasm Invasiveness Antibodies  >228 publications with MAPK1 and Neoplasm Invasiveness
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >1850 publications with MAPK1 and Blood
 Vascular Antibodies  >1790 publications with MAPK1 and Vascular
 Muscle Antibodies  >1632 publications with MAPK1 and Muscle
 Brain Antibodies  >1331 publications with MAPK1 and Brain
 Heart Antibodies  >761 publications with MAPK1 and Heart
 Lung Antibodies  >673 publications with MAPK1 and Lung
 Bone Antibodies  >573 publications with MAPK1 and Bone
 Kidney Antibodies  >570 publications with MAPK1 and Kidney
 Liver Antibodies  >563 publications with MAPK1 and Liver
 Nerve Antibodies  >497 publications with MAPK1 and Nerve
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