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anti-MAPK3 antibody :: Rabbit MAPK3 Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS9600418
Unit / Price
0.1 mL  /  $255 +1 FREE 8GB USB
0.2 mL  /  $295 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

MAPK3, Polyclonal Antibody

★Popular Item★
 Also Known As   

MAPK3 Antibody

 Product Synonym Names    3pK; anti MAPKAPK3; Chromosome 3p kinase; EC 2.7.11.1; MAP kinase-activated protein kinase 3; MAPK activated protein kinase 3; MAPK-activated protein kinase 3; MAPK3_HUMAN; MAPKAP; MAPKAP kinase 3; MAPKAP-K3; MAPKAP3; MAPKAPK 3; MAPKAPK-3; Mapkapk3; MAPKAPK3 antibody; Mitogen activated protein kinase activated protein kinase 3; Mitogen activated protein kinase activated protein kinase; MK-3
 Product Gene Name   

anti-MAPK3 antibody

[Similar Products]
 Product Synonym Gene Name    MAPKAPK3 [Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 3D Structure    ModBase 3D Structure for Q16644
 Clonality    Polyclonal
 Isotype    IgG
 Host    Rabbit
 Species Reactivity    Human, Mouse, Rat
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 Specificity    MAPK3 antibody detects endogenous levels of MAPK3
 Purity/Purification    The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
 Form/Format    Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
 Concentration    1mg/ml (lot specific)
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 Immunogen    A synthesized peptide derived from human MAPK3
 Subcellular Location    Nucleus. Cytoplasm. Predominantly located in the nucleus, when activated it translocates to the cytoplasm.
 Tissue Specificity    Widely expressed, with a higher expression level observed in heart and skeletal muscle. No expression in brain. Expressed in the retinal pigment epithelium (PubMed:26744326).
 Conjugation    Unconjugated
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 Preparation and Storage    Store at -20 degree C. Stable for 12 months from date of receipt.
 Other Notes    Small volumes of anti-MAPK3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-MAPK3 antibody

   Description: This gene encodes a member of the Ser/Thr protein kinase family. This kinase functions as a mitogen-activated protein kinase (MAP kinase)-activated protein kinase. MAP kinases are also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This kinase was shown to be activated by growth inducers and stress stimulation of cells. In vitro studies demonstrated that ERK, p38 MAP kinase and Jun N-terminal kinase were all able to phosphorylate and activate this kinase, which suggested the role of this kinase as an integrative element of signaling in both mitogen and stress responses. This kinase was reported to interact with, phosphorylate and repress the activity of E47, which is a basic helix-loop-helix transcription factor known to be involved in the regulation of tissue-specific gene expression and cell differentiation.
Function: Stress-activated serine/threonine-protein kinase involved in cytokines production, endocytosis, cell migration, chromatin remodeling and transcriptional regulation. Following stress, it is phosphorylated and activated by MAP kinase p38-alpha/MAPK14, leading to phosphorylation of substrates. Phosphorylates serine in the peptide sequence, Hyd-X-R-X2-S, where Hyd is a large hydrophobic residue. MAPKAPK2 and MAPKAPK3, share the same function and substrate specificity, but MAPKAPK3 kinase activity and level in protein expression are lower compared to MAPKAPK2. Phosphorylates HSP27/HSPB1, KRT18, KRT20, RCSD1, RPS6KA3, TAB3 and TTP/ZFP36. Mediates phosphorylation of HSP27/HSPB1 in response to stress, leading to dissociate HSP27/HSPB1 from large small heat-shock protein (sHsps) oligomers and impair their chaperone activities and ability to protect against oxidative stress effectively. Involved in inflammatory response by regulating tumor necrosis factor (TNF) and IL6 production post-transcriptionally: acts by phosphorylating AU-rich elements (AREs)-binding proteins, such as TTP/ZFP36, leading to regulate the stability and translation of TNF and IL6 mRNAs. Phosphorylation of TTP/ZFP36, a major post-transcriptional regulator of TNF, promotes its binding to 14-3-3 proteins and reduces its ARE mRNA affinity leading to inhibition of dependent degradation of ARE-containing transcript. Involved in toll-like receptor signaling pathway (TLR) in dendritic cells: required for acute TLR-induced macropinocytosis by phosphorylating and activating RPS6KA3. Also acts as a modulator of Polycomb-mediated repression.
Subunit Structure: Heterodimer with p38-alpha/MAPK14. The heterodimer with p38-alpha/MAPK14 forms a stable complex: molecules are positioned 'face to face' so that the ATP-binding sites of both kinases are at the heterodimer interface (By similarity). Interacts with TCF3 and with polycomb proteins, such as PCH2 and BMI1/PCGF4.
Post-translational Modifications: Phosphorylated and activated by MAPK1/ERK2 and MAPK3/ERK1. Phosphorylated and activated by MAP kinase p38-alpha/MAPK14 at Thr-201, Ser-251 and Thr-313 (By similarity).
Similarity: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
 Applications Tested/Suitable for anti-MAPK3 antibody   

Western Blot (WB), Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)

 Application Notes for anti-MAPK3 antibody    WB: 1:500-1:2000
IHC: 1:50-1:200
IF/ICC: 1:100-1:500
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 Immunohistochemistry (IHC) of anti-MAPK3 antibody    MBS9600418 at 1/100 staining human Smooth muscle tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
anti-MAPK3 antibody Immunohistochemistry (IHC) (IHC) image
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 Immunofluorescene (IF) of anti-MAPK3 antibody    MBS9600418 staining COLO205 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
anti-MAPK3 antibody Immunofluorescene (IF) image
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 Western Blot (WB) of anti-MAPK3 antibody    Western blot analysis on COLO205 cell lysate using MAPK3 Antibody, The lane on the left is treated with the antigen-specific peptide.
anti-MAPK3 antibody Western Blot (WB) (WB) image
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NCBI/Uniprot data below describe general gene information for MAPK3. It may not necessarily be applicable to this product.
 NCBI GI #    345441756
 NCBI GeneID    7867
 NCBI Accession #    NP_001230854.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001243925.1 [Other Products]
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 UniProt Primary Accession #    Q16644 [Other Products]
 UniProt Secondary Accession #    B5BU67 [Other Products]
 UniProt Related Accession #    Q16644 [Other Products]
 Molecular Weight    Observed: 42 kDa
Predicted: 43 kDa
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 NCBI Official Full Name    MAP kinase-activated protein kinase 3
 NCBI Official Synonym Full Names    mitogen-activated protein kinase-activated protein kinase 3
 NCBI Official Symbol    MAPKAPK3 [Similar Products]
 NCBI Official Synonym Symbols   
3PK; MK-3; MDPT3; MAPKAP3; MAPKAP-K3; MAPKAPK-3
[Similar Products]
 NCBI Protein Information    MAP kinase-activated protein kinase 3
 UniProt Protein Name    MAP kinase-activated protein kinase 3
 UniProt Synonym Protein Names   
Chromosome 3p kinase; 3pK
 UniProt Gene Name    MAPKAPK3 [Similar Products]
 UniProt Synonym Gene Names    MAPK-activated protein kinase 3; MAPKAP kinase 3; MAPKAP-K3; MAPKAPK-3; MK-3; 3pK [Similar Products]
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 NCBI Summary for MAPK3    This gene encodes a member of the Ser/Thr protein kinase family. This kinase functions as a mitogen-activated protein kinase (MAP kinase)- activated protein kinase. MAP kinases are also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This kinase was shown to be activated by growth inducers and stress stimulation of cells. In vitro studies demonstrated that ERK, p38 MAP kinase and Jun N-terminal kinase were all able to phosphorylate and activate this kinase, which suggested the role of this kinase as an integrative element of signaling in both mitogen and stress responses. This kinase was reported to interact with, phosphorylate and repress the activity of E47, which is a basic helix-loop-helix transcription factor known to be involved in the regulation of tissue-specific gene expression and cell differentiation. Alternate splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Sep 2011]
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 UniProt Comments for MAPK3    Stress-activated serine/threonine-protein kinase involved in cytokines production, endocytosis, cell migration, chromatin remodeling and transcriptional regulation. Following stress, it is phosphorylated and activated by MAP kinase p38-alpha/MAPK14, leading to phosphorylation of substrates. Phosphorylates serine in the peptide sequence, Hyd-X-R-X2-S, where Hyd is a large hydrophobic residue. MAPKAPK2 and MAPKAPK3, share the same function and substrate specificity, but MAPKAPK3 kinase activity and level in protein expression are lower compared to MAPKAPK2. Phosphorylates HSP27/HSPB1, KRT18, KRT20, RCSD1, RPS6KA3, TAB3 and TTP/ZFP36. Mediates phosphorylation of HSP27/HSPB1 in response to stress, leading to dissociate HSP27/HSPB1 from large small heat-shock protein (sHsps) oligomers and impair their chaperone activities and ability to protect against oxidative stress effectively. Involved in inflammatory response by regulating tumor necrosis factor (TNF) and IL6 production post-transcriptionally: acts by phosphorylating AU-rich elements (AREs)-binding proteins, such as TTP/ZFP36, leading to regulate the stability and translation of TNF and IL6 mRNAs. Phosphorylation of TTP/ZFP36, a major post-transcriptional regulator of TNF, promotes its binding to 14-3-3 proteins and reduces its ARE mRNA affinity leading to inhibition of dependent degradation of ARE-containing transcript. Involved in toll-like receptor signaling pathway (TLR) in dendritic cells: required for acute TLR-induced macropinocytosis by phosphorylating and activating RPS6KA3. Also acts as a modulator of Polycomb-mediated repression.
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 Research Articles on MAPK3    1. The natural history of MCRPE is in relation to the role of MAPKAPK3 in BM modeling, vascular endothelial growth factor activity, retinal pigment epithelial responses
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-MAPK3 antibodyPathways associated with anti-MAPK3 antibody
 Reference Product  PubMed Publications
 MAPK14 antibody  >3 publications with MAPK3 and MAPK14
 HSPB1 antibody  >2 publications with MAPK3 and HSPB1
 EEF2K antibody  >1 publications with MAPK3 and EEF2K
 EEF2 antibody  >1 publications with MAPK3 and EEF2
 PHC2 antibody  >1 publications with MAPK3 and PHC2
 BMI1 antibody  >1 publications with MAPK3 and BMI1
 Products by Pathway  Pathway Diagram
 Activated TLR4 Signalling Pathway antibodies  Activated TLR4 Signalling Pathway Diagram
 FAS Pathway And Stress Induction Of HSP Regulation antibodies  FAS Pathway And Stress Induction Of HSP Regulation Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Innate Immune System Pathway antibodies  Innate Immune System Pathway Diagram
 MAP Kinase Activation In TLR Cascade Pathway antibodies  MAP Kinase Activation In TLR Cascade Pathway Diagram
 MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
 MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
 MyD88 Cascade Initiated On Plasma Membrane Pathway antibodies  MyD88 Cascade Initiated On Plasma Membrane Pathway Diagram
 MyD88 Dependent Cascade Initiated On Endosome Pathway antibodies  MyD88 Dependent Cascade Initiated On Endosome Pathway Diagram
 MyD88-independent Cascade Pathway antibodies  MyD88-independent Cascade Pathway Diagram
Diseases associated with anti-MAPK3 antibodyOrgans/Tissues associated with anti-MAPK3 antibody
 Disease Name  Pubmed Publications
 Necrosis Antibodies  >8 publications with MAPK3 and Necrosis
 Inflammation Antibodies  >4 publications with MAPK3 and Inflammation
 Gastrointestinal Diseases Antibodies  >2 publications with MAPK3 and Gastrointestinal Diseases
 Liver Diseases Antibodies  >2 publications with MAPK3 and Liver Diseases
 Lung Neoplasms Antibodies  >2 publications with MAPK3 and Lung Neoplasms
 Disease Models, Animal Antibodies  >2 publications with MAPK3 and Disease Models, Animal
 Hepatitis Antibodies  >2 publications with MAPK3 and Hepatitis
 Liver Neoplasms Antibodies  >1 publications with MAPK3 and Liver Neoplasms
 Death Antibodies  >1 publications with MAPK3 and Death
 Heart Diseases Antibodies  >1 publications with MAPK3 and Heart Diseases
 Organ/Tissue Name  Pubmed Publications
 Embryonic Tissue Antibodies  >1 publications with MAPK3 and Embryonic Tissue
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