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anti-MAPK8 antibody :: Rabbit MAPK8 Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS9200829
Unit / Price
0.08 mL  /  $150 +1 FREE 8GB USB
0.4 mL  /  $340 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

MAPK8, Polyclonal Antibody

★Popular Item★
 Also Known As   

MAPK8 Antibody (T183/Y185)

 Product Synonym Names    Mitogen-activated protein kinase 8; MAP kinase 8; MAPK 8; JNK-46; Stress-activated protein kinase 1c; SAPK1c; Stress-activated protein kinase JNK1; c-Jun N-terminal kinase 1; MAPK8; JNK1; PRKM8; SAPK1; SAPK1C
 Product Gene Name   

anti-MAPK8 antibody

[Similar Products]
 Antibody/Peptide Pairs    MAPK8 (T183/Y185) peptide (MBS9217988) is used for blocking the activity of MAPK8 antibody (MBS9200829)
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Immunogen Sequence Positions    159-195
 OMIM    601158
 3D Structure    ModBase 3D Structure for P45983
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 Clonality    Polyclonal
 Isotype    Rabbit Ig
 Host    Rabbit
 Species Reactivity    Human (Predicted Reactivity: Mouse, Rat, Xenopus)
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 Specificity    This MAPK8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 159-195 amino acids from human MAPK8.
 Purity/Purification    Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
 Form/Format    Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
 Concentration    Vial Concentration: 0.45 (lot specific)
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 Antigen Type    Synthetic Peptide
 Other Accession    P49187, Q61831, P53779, Q8QHK8, P49185, Q91Y86, NP_620637
 Antigen Source    HUMAN
 Cellular Location    Cytoplasm. Nucleus
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 Preparation and Storage    Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
 Other Notes    Small volumes of anti-MAPK8 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-MAPK8 antibody

   Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation and programmed cell death. Extracellular stimuli such as proinflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK8/JNK1. In turn, MAPK8/JNK1 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. Phosphorylates the replication licensing factor CDT1, inhibiting the interaction between CDT1 and the histone H4 acetylase HBO1 to replication origins. Loss of this interaction abrogates the acetylation required for replication initiation. Promotes stressed cell apoptosis by phosphorylating key regulatory factors including p53/TP53 and Yes-associates protein YAP1. In T-cells, MAPK8 and MAPK9 are required for polarized differentiation of T-helper cells into Th1 cells. Contributes to the survival of erythroid cells by phosphorylating the antagonist of cell death BAD upon EPO stimulation. Mediates starvation-induced BCL2 phosphorylation, BCL2 dissociation from BECN1, and thus activation of autophagy. Phosphorylates STMN2 and hence regulates microtubule dynamics, controlling neurite elongation in cortical neurons. In the developing brain, through its cytoplasmic activity on STMN2, negatively regulates the rate of exit from multipolar stage and of radial migration from the ventricular zone. Phosphorylates several other substrates including heat shock factor protein 4 (HSF4), the deacetylase SIRT1, ELK1, or the E3 ligase ITCH. Phosphorylates the CLOCK-ARNTL/BMAL1 heterodimer and plays a role in the regulation of the circadian clock

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation. Four alternatively spliced transcript variants encoding distinct isoforms have been reported.
 Product Categories/Family for anti-MAPK8 antibody    Cancer; Immunology; Neuroscience; Signal Transduction
 Applications Tested/Suitable for anti-MAPK8 antibody   

ELISA (EIA), Immunohistochemistry-Paraffin(IHC-P), Western Blot (WB)

 Application Notes for anti-MAPK8 antibody    WB~~1:1000
IHC-P~~1:50~100
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 Western Blot (WB) of anti-MAPK8 antibody    Western blot analysis of lysate from Hela cell line, using JNK1 antibody. MBS9200829 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.
anti-MAPK8 antibody Western Blot (WB) (WB) image
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 Immunohistochemistry (IHC) of anti-MAPK8 antibody    JNK1-T183/Y185 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the LJNK1-T183/Y185 antibody for immunohistochemistry. Clinical relevance has not been evaluated.
anti-MAPK8 antibody Immunohistochemistry (IHC) (IHC) image
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NCBI/Uniprot data below describe general gene information for MAPK8. It may not necessarily be applicable to this product.
 NCBI GI #    513788279
 NCBI GeneID    5599
 NCBI Accession #    NP_001265476.1 [Other Products]
 NCBI Related Accession #    NP_002741.1; NP_620634.1; NP_620637.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001278547.1 [Other Products]
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 UniProt Primary Accession #    P45983 [Other Products]
 UniProt Secondary Accession #    Q15709; Q15712; Q15713; Q308M2; B5BTZ5; B7ZLV4; D3DX88; D3DX92 [Other Products]
 UniProt Related Accession #    P45983 [Other Products]
 Molecular Weight    48296
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 NCBI Official Full Name    mitogen-activated protein kinase 8 JNK1 beta2
 NCBI Official Synonym Full Names    mitogen-activated protein kinase 8
 NCBI Official Symbol    MAPK8 [Similar Products]
 NCBI Official Synonym Symbols   
JNK; JNK1; PRKM8; SAPK1; JNK-46; JNK1A2; SAPK1c; JNK21B1/2
[Similar Products]
 NCBI Protein Information    mitogen-activated protein kinase 8
 UniProt Protein Name    Mitogen-activated protein kinase 8
 UniProt Synonym Protein Names   
JNK-46; Stress-activated protein kinase 1c; SAPK1c; Stress-activated protein kinase JNK1; c-Jun N-terminal kinase 1
 UniProt Gene Name    MAPK8 [Similar Products]
 UniProt Synonym Gene Names    JNK1; PRKM8; SAPK1; SAPK1C; MAP kinase 8; MAPK 8; SAPK1c [Similar Products]
 UniProt Entry Name    MK08_HUMAN
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 NCBI Summary for MAPK8    The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation. Five alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jun 2013]
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 UniProt Comments for MAPK8    JNK1: a protein kinase of the MAPK family that is potently activated by a variety of environmental stresses, including UV and gamma radiation, ceramides, pro-inflammatory cytokines and, in some instances, by growth factors and GPCR agonists. Substrates include a number of transcription factors, primarily components of AP-1 such as c-Jun and ATF2, thus regulating AP-1 transcriptional activity. In T- cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells. Binds to at least four scaffolding proteins, JIP-1, -2, -3 and -4. Activity increased in obesity. Inhibition or mouse knockout increases insulin sensitivity. Part of NFB pathway involved in inflammation and cancer, and signals downstream of Ras, though possibly as an apoptotic negative regulator of growth. Four alternatively-spliced isoforms have been described.

Protein type: Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.24; Kinase, protein; Protein kinase, CMGC; CMGC group; MAPK family; MAPK/JNK subfamily; JNK subfamily

Chromosomal Location of Human Ortholog: 10q11.22

Cellular Component: nucleoplasm; mitochondrion; nucleus; cytosol

Molecular Function: protein serine/threonine kinase activity; protein binding; enzyme binding; histone deacetylase regulator activity; histone deacetylase binding; JUN kinase activity; ATP binding

Biological Process: nerve growth factor receptor signaling pathway; apoptosis; positive regulation of apoptosis; stress-activated MAPK cascade; rhythmic process; toll-like receptor 3 signaling pathway; protein amino acid phosphorylation; toll-like receptor 10 signaling pathway; toll-like receptor 5 signaling pathway; regulation of protein localization; regulation of transcription factor activity; JNK cascade; response to stress; negative regulation of protein binding; toll-like receptor 4 signaling pathway; response to UV; regulation of histone deacetylation; ossification; MyD88-independent toll-like receptor signaling pathway; peptidyl-threonine phosphorylation; regulation of circadian rhythm; toll-like receptor 2 signaling pathway; JUN phosphorylation; MyD88-dependent toll-like receptor signaling pathway; peptidyl-serine phosphorylation; response to cadmium ion; toll-like receptor signaling pathway; innate immune response; toll-like receptor 9 signaling pathway; negative regulation of apoptosis
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Product References and Citations for anti-MAPK8 antibody

   Dasgupta, J., et al. J. Cell. Physiol. 225(1):52-62(2010)
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Gao, D., et al. J. Biol. Chem. 285(39):29965-29973(2010)
Han, J.S., et al. Anticancer Res. 30(9):3407-3412(2010)
Yang, X.H., et al. World J. Gastroenterol. 16(27):3385-3393(2010)
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 Research Articles on MAPK8    1. Phosphorylation of sterol regulatory element-binding protein (SREBP)-1c by p38 kinases, ERK and JNK
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-MAPK8 antibodyPathways associated with anti-MAPK8 antibody
 Reference Product  PubMed Publications
 JUN antibody  >839 publications with MAPK8 and JUN
 FOS antibody  >45 publications with MAPK8 and FOS
 MAP2K4 antibody  >31 publications with MAPK8 and MAP2K4
 IRS1 antibody  >23 publications with MAPK8 and IRS1
 ATF2 antibody  >23 publications with MAPK8 and ATF2
 MAP2K7 antibody  >19 publications with MAPK8 and MAP2K7
 BCL2 antibody  >18 publications with MAPK8 and BCL2
 MAPK8IP1 antibody  >13 publications with MAPK8 and MAPK8IP1
 JUND antibody  >12 publications with MAPK8 and JUND
 TP53 antibody  >7 publications with MAPK8 and TP53
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Diseases associated with anti-MAPK8 antibodyOrgans/Tissues associated with anti-MAPK8 antibody
 Disease Name  Pubmed Publications
 Necrosis Antibodies  >172 publications with MAPK8 and Necrosis
 Inflammation Antibodies  >131 publications with MAPK8 and Inflammation
 Nervous System Diseases Antibodies  >85 publications with MAPK8 and Nervous System Diseases
 Disease Models, Animal Antibodies  >68 publications with MAPK8 and Disease Models, Animal
 Liver Diseases Antibodies  >61 publications with MAPK8 and Liver Diseases
 Brain Diseases Antibodies  >58 publications with MAPK8 and Brain Diseases
 Breast Neoplasms Antibodies  >37 publications with MAPK8 and Breast Neoplasms
 Reperfusion Injury Antibodies  >37 publications with MAPK8 and Reperfusion Injury
 Cell Transformation, Neoplastic Antibodies  >36 publications with MAPK8 and Cell Transformation, Neoplastic
 Heart Diseases Antibodies  >34 publications with MAPK8 and Heart Diseases
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >168 publications with MAPK8 and Blood
 Brain Antibodies  >148 publications with MAPK8 and Brain
 Vascular Antibodies  >96 publications with MAPK8 and Vascular
 Lung Antibodies  >75 publications with MAPK8 and Lung
 Heart Antibodies  >70 publications with MAPK8 and Heart
 Bone Antibodies  >41 publications with MAPK8 and Bone
 Skin Antibodies  >38 publications with MAPK8 and Skin
 Kidney Antibodies  >37 publications with MAPK8 and Kidney
 Connective Tissue Antibodies  >31 publications with MAPK8 and Connective Tissue
 Prostate Antibodies  >28 publications with MAPK8 and Prostate
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