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anti-MARCH10 antibody :: Goat MARCH10 Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS421725
Western Blot (WB)
Unit / Price
0.1 mg  /  $300 +1 FREE 8GB USB
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

MARCH10, Polyclonal Antibody

 Also Known As   

Goat anti-MARCH10 Antibody

 Product Synonym Names    MARCH10; membrane-associated ring finger (C3HC4) 10; FLJ35757; MARCH-X; RNF190; ring finger protein 190; MARCH10 antibody; membrane-associated ring finger (C3HC4) 10 antibody; FLJ35757 antibody; MARCH-X antibody; RNF190 antibody; ring finger protein 190 antibody
 Product Gene Name   

anti-MARCH10 antibody

[Similar Products]
 Antibody/Peptide Pairs    MARCH10 peptide (MBS425084) is used for blocking the activity of MARCH10 antibody (MBS421725)
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Immunogen Sequence    SRNYPQPRTE E
 OMIM    613337
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 Clonality    Polyclonal
 Host    Goat
 Species Reactivity    Tested: Rat; Expected from sequence similarity: Human, Rat, Cow
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 Purity/Purification    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
 Form/Format    Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
 Concentration    100ug specific antibody in 200ul (lot specific)
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 Immunogen    Peptide with sequence C-SRNYPQPRTEE, from the internal region of the protein sequence according to NP_001094345.1; NP_689811.2.
 Epitope    Internal region
 Note    This antibody is expected to recognise both reported isoforms (NP_001094345.1; NP_689811.2), which are identical.
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 Preparation and Storage    Aliquot and store at -20 degree C. Minimize freezing and thawing.
 ISO Certification    Manufactured in an ISO 9001:2015 Certified Laboratory.
 Other Notes    Small volumes of anti-MARCH10 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Applications Tested/Suitable for anti-MARCH10 antibody   

Peptide ELISA (EIA), Western Blot (WB)

 Application Notes for anti-MARCH10 antibody    Peptide ELISA: Antibody detection limit dilution 1: 8000.
Western Blot: Approx.90 kDa band observed in Rat Testis lysates (calculated MW of 90.5 kDa according to Human NP_689811.2 and of 87.9 kDa according to Mouse NP_766156.2). Recommended concentration: 0.3-1ug/ml. An additional band of unknown identity was also consistently observed at 37 kDa. This band was successfully blocked by incubation with the immunising peptide.
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 Western Blot (WB) of anti-MARCH10 antibody    (0.3ug/ml) staining of Rat Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
anti-MARCH10 antibody Western Blot (WB) (WB) image
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NCBI/Uniprot data below describe general gene information for MARCH10. It may not necessarily be applicable to this product.
 NCBI GI #    154937342
 NCBI GeneID    162333
 NCBI Accession #    NP_001094345.1 [Other Products]
 NCBI Related Accession #    NP_689811.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_001100875.2 [Other Products]
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 UniProt Secondary Accession #    Q8IYS7; Q8N7Z7; D3DU09 [Other Products]
 UniProt Related Accession #    Q8NA82 [Other Products]
 Molecular Weight    90,511 Da
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 NCBI Official Full Name    probable E3 ubiquitin-protein ligase MARCH10 isoform 1
 NCBI Official Synonym Full Names    membrane associated ring-CH-type finger 10
 NCBI Official Symbol    MARCH10 [Similar Products]
 NCBI Official Synonym Symbols   
[Similar Products]
 NCBI Protein Information    probable E3 ubiquitin-protein ligase MARCH10
 UniProt Protein Name    Probable E3 ubiquitin-protein ligase MARCH10
 UniProt Synonym Protein Names   
Membrane-associated RING finger protein 10; Membrane-associated RING-CH protein X; MARCH-X; RING finger protein 190
 UniProt Gene Name    MARCH10 [Similar Products]
 UniProt Synonym Gene Names    RNF190; MARCH-X [Similar Products]
 UniProt Entry Name    MARHA_HUMAN
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 NCBI Summary for MARCH10    MARCH10 is a member of the MARCH family of membrane-bound E3 ubiquitin ligases (EC MARCH enzymes add ubiquitin (see MIM 191339) to target lysines in substrate proteins, thereby signaling their vesicular transport between membrane compartments (Morokuma et al., 2007 [PubMed 17604280]).[supplied by OMIM, Apr 2010]
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 UniProt Comments for MARCH10    MARCH10: E3 ubiquitin-protein ligase (Probable). E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates.

Protein type: Ubiquitin ligase; Ubiquitin conjugating system; EC 6.3.2.-; Ligase; EC

Chromosomal Location of Human Ortholog: 17q23.2

Molecular Function: ligase activity; protein binding; zinc ion binding

Biological Process: protein ubiquitination
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 Research Articles on MARCH10    1. Observational study of gene-disease association. (HuGE Navigator)
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Organs/Tissues associated with anti-MARCH10 antibody
 Organ/Tissue Name  Pubmed Publications
 Testis Antibodies  >1 publications with MARCH10 and Testis
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