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anti-MCP-1 antibody :: Rabbit MCP-1 Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS551047
Immunohistochemistry
Unit / Price
0.1 mg  /  $280 +1 FREE 8GB USB
0.2 mg  /  $385 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

MCP-1, Polyclonal Antibody

★Popular Item★
 Also Known As   

Rabbit anti MCP-1 Polyclonal

 Product Synonym Names    Rabbit anti MCP-1 Polyclonal purified; mcp-1; mcp1; ccl2; ccl-2; pab; polyclonal
 Product Gene Name   

anti-MCP-1 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    158105
 3D Structure    ModBase 3D Structure for P13500
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 Clonality    Polyclonal
 Isotype    IgG
 Host    Rabbit
 Species Reactivity    Human, Mouse, Rat
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 Purity/Purification    >95%

Purification:
Serum IgG fraction was purified by Protein G affinity chromatography.
 Form/Format    Lyophilized from 0.2um filtered solution in PBS pH 7.4
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 Source Note    Polyclonal MCP-1 (CCL2) antibody was produced from sera of rabbits immunized with synthetic protein containing MCP-1 antigenic determinants.
 Reconstitution    A quick spin of the vial followed by reconstitution in distilled water. This solution can then be diluted into other buffers
 Dilution    A quick spin of the vial followed by reconsititution in 0.01M PBS pH 7.4 or other diluents.
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 Preparation and Storage    The lyophilized antibody is stable for at least 1 year from date of receipt at -20 degree C.
Upon reconstitution, this antibody can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months without detectable loss of activity.

Avoid repeated freeze/thaw cycles.
Shelf Life: >12 months
 Other Notes    Small volumes of anti-MCP-1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-MCP-1 antibody

   MCP-1 belongs to the family of chemotactic cytokines.MCP-1 is expressed by monocytes, vascular endothelial cells, smooth muscle cells, glomerular mesangial cell, osteoblastic cells. MCP-1 has been shown to exhibit biological activities other than Chemotaxis. It can induce the proliferation and activation of killer cells known as CHAK. Elevated levels of MCP-1 are observed in atherosclerotic plaques that are rich in macrophages. The factor activates the tumoricidal activity of monocytes and macrophages in vivo. It regulates the expression of cell surface antigens (CD11c, CD11b) and the expression of cytokines (IL1, IL6). MCP-1 is a potent activator of human basophils, inducing degranulation and the release of histamines. In basophils activated by IL3, IL5 or GM-CSF MCP-1 enhances the synthesis of leukotriene C4.
 Product Categories/Family for anti-MCP-1 antibody    Polyclonal
 Applications Tested/Suitable for anti-MCP-1 antibody   

Western Blot (WB), IHC (Immunohistochemistry), ELISA (EIA)

 Application Notes for anti-MCP-1 antibody    ELISA: Use at a concentration range of 1-2ug/ml.
Western blot:A suitable range of concentrations of this antibody for WB detection is 2-10 ug/ml.
IHC: A suitable range of concentrations of this antibody for IHC is 2-10 ug/ml.
Immunohistochemical analysis of MCP-1 expression in paraffin fixed obese mouse proximal tubule tissue.
It was used as primary antibody, dilution 1:400.
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 Immunohistochemistry of anti-MCP-1 antibody    anti-MCP-1 antibody Immunohistochemistry (IHC) image
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NCBI/Uniprot data below describe general gene information for MCP-1. It may not necessarily be applicable to this product.
 NCBI GI #    4506841
 NCBI GeneID    6347
 NCBI Accession #    NP_002973.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_002982.3 [Other Products]
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 UniProt Primary Accession #    P13500 [Other Products]
 UniProt Secondary Accession #    Q9UDF3; B2R4V3 [Other Products]
 UniProt Related Accession #    P13500; Q6UZ82 [Other Products]
 Molecular Weight    11,025 Da
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 NCBI Official Full Name    C-C motif chemokine 2
 UniProt Protein Name    C-C motif chemokine 2
 UniProt Synonym Protein Names   
HC11; Monocyte chemoattractant protein 1; Monocyte chemotactic and activating factor; MCAF; Monocyte chemotactic protein 1; MCP-1; Monocyte secretory protein JE; Small-inducible cytokine A2
 Protein Family    Major capsid protein
 UniProt Gene Name    CCL2 [Similar Products]
 UniProt Synonym Gene Names    MCP1; SCYA2; MCAF; MCP-1 [Similar Products]
 UniProt Entry Name    CCL2_HUMAN
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 NCBI Summary for MCP-1    This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of N-terminal cysteine residues of the mature peptide. This chemokine is a member of the CC subfamily which is characterized by two adjacent cysteine residues. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4. [provided by RefSeq, Jul 2013]
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 UniProt Comments for MCP-1    CCL2: Chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. Monomer or homodimer; in equilibrium. Binds to CCR2 and CCR4. Is tethered on endothelial cells by glycosaminoglycan (GAG) side chains of proteoglycans. Belongs to the intercrine beta (chemokine CC) family.

Protein type: Chemokine; Secreted; Secreted, signal peptide; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 17q11.2-q12

Cellular Component: extracellular space; rough endoplasmic reticulum; perinuclear region of cytoplasm; endocytic vesicle; dendrite; extracellular region; synapse; perikaryon; nerve terminal

Molecular Function: heparin binding; CCR2 chemokine receptor binding; chemokine activity; receptor binding; protein kinase activity

Biological Process: maternal process involved in pregnancy; protein amino acid phosphorylation; response to antibiotic; regulation of cell shape; monocyte chemotaxis; cell surface receptor linked signal transduction; transforming growth factor beta receptor signaling pathway; response to vitamin B3; negative regulation of neuron apoptosis; cellular homeostasis; cell adhesion; neutrophil chemotaxis; organ regeneration; JAK-STAT cascade; positive regulation of tumor necrosis factor production; response to amino acid stimulus; G-protein signaling, coupled to cyclic nucleotide second messenger; organ morphogenesis; response to ethanol; unfolded protein response, activation of signaling protein activity; cellular response to insulin stimulus; response to bacterium; response to mechanical stimulus; response to heat; positive regulation of endothelial cell proliferation; response to activity; response to progesterone stimulus; positive regulation of nitric-oxide synthase biosynthetic process; positive regulation of collagen biosynthetic process; signal transduction; chemotaxis; positive regulation of synaptic transmission; positive regulation of cellular extravasation; response to wounding; lipopolysaccharide-mediated signaling pathway; protein kinase B signaling cascade; response to gamma radiation; angiogenesis; inflammatory response; lymphocyte chemotaxis; aging; unfolded protein response; cytokine and chemokine mediated signaling pathway; MAPKKK cascade; cytoskeleton organization and biogenesis; viral genome replication; macrophage chemotaxis; leukocyte migration during inflammatory response; humoral immune response; cellular calcium ion homeostasis; G-protein coupled receptor protein signaling pathway; positive regulation of leukocyte mediated cytotoxicity; negative regulation of angiogenesis; cellular protein metabolic process; maternal process involved in parturition; response to hypoxia; positive regulation of T cell activation; vascular endothelial growth factor receptor signaling pathway; astrocyte cell migration

Disease: Neural Tube Defects; Mycobacterium Tuberculosis, Susceptibility To; Human Immunodeficiency Virus Type 1, Susceptibility To
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Product References and Citations for anti-MCP-1 antibody

   rationally evolving mcp-1/ccl2 into a decoy protein with potent anti-inflammatory activity in vivo .J. Biol. Chem., Mar 2010; 285: 8782 - 8792.
association of the mcp-1 -2518 a/g polymorphism and no association of its receptor ccr2 -64 v/i polymorphism with lupus nephritis .J Rheumatol, Mar 2010; 10.3899/jrheum.090681.
mcp-1/ccl2 modifies axon properties in a pmp22-overexpressing mouse model for charcot-marie-tooth 1a neuropathy .Am. J. Pathol., Mar 2010; 176: 1390 - 1399.
critical protective role for mcp-1 in pneumonic burkholderia mallei infection .J. Immunol., Feb 2010; 184: 1445 - 1454.
thioredoxin 1 downregulates mcp-1 secretion in human endothelial cells by suppressing nuclear translocation of activator protein 1 and redox factor-1 .Am J Physiol Cell Physiol, Dec 2009; 10.1152/ajpcell.00223.2009.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-MCP-1 antibodyPathways associated with anti-MCP-1 antibody
 Reference Product  PubMed Publications
 CCR2 antibody  >860 publications with MCP-1 and CCR2
 CCR5 antibody  >261 publications with MCP-1 and CCR5
 CCR1 antibody  >146 publications with MCP-1 and CCR1
 CCR10 antibody  >13 publications with MCP-1 and CCR10
 DARC antibody  >12 publications with MCP-1 and DARC
 MMP3 antibody  >5 publications with MCP-1 and MMP3
 Products by Pathway  Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
 Chemokine Receptors Bind Chemokines Pathway antibodies  Chemokine Receptors Bind Chemokines Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Chemokine Signaling Pathway antibodies  Chemokine Signaling Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Diabetes Pathways antibodies  Diabetes Pathways Diagram
 GPCR Ligand Binding Pathway antibodies  GPCR Ligand Binding Pathway Diagram
Diseases associated with anti-MCP-1 antibodyOrgans/Tissues associated with anti-MCP-1 antibody
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >2838 publications with MCP-1 and Inflammation
 Cardiovascular Diseases Antibodies  >1352 publications with MCP-1 and Cardiovascular Diseases
 Atherosclerosis Antibodies  >792 publications with MCP-1 and Atherosclerosis
 Kidney Diseases Antibodies  >602 publications with MCP-1 and Kidney Diseases
 Neoplasms Antibodies  >582 publications with MCP-1 and Neoplasms
 Lung Diseases Antibodies  >541 publications with MCP-1 and Lung Diseases
 Fibrosis Antibodies  >451 publications with MCP-1 and Fibrosis
 Coronary Artery Disease Antibodies  >249 publications with MCP-1 and Coronary Artery Disease
 Hypertension Antibodies  >245 publications with MCP-1 and Hypertension
 Drug Toxicity Antibodies  >243 publications with MCP-1 and Drug Toxicity
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >4123 publications with MCP-1 and Blood
 Vascular Antibodies  >1895 publications with MCP-1 and Vascular
 Kidney Antibodies  >825 publications with MCP-1 and Kidney
 Lung Antibodies  >758 publications with MCP-1 and Lung
 Muscle Antibodies  >752 publications with MCP-1 and Muscle
 Brain Antibodies  >615 publications with MCP-1 and Brain
 Bone Antibodies  >455 publications with MCP-1 and Bone
 Heart Antibodies  >447 publications with MCP-1 and Heart
 Liver Antibodies  >399 publications with MCP-1 and Liver
 Skin Antibodies  >307 publications with MCP-1 and Skin
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