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anti-MAP2K1 antibody :: Rabbit anti-Recombinant MEK1 (Thr292) Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS502072
Testing Data
Unit / Price
0.1 mL  /  $360 +1 FREE 8GB USB
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

MEK1 (Thr292) (MAP2K1), Polyclonal Antibody

★Popular Item★
 Also Known As   

Anti-Phospho-Thr292 MEK 1

 Product Gene Name   

anti-MAP2K1 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Chromosome Location    Chromosome: 15; NC_000015.10 (66386844..66491544). Location: 15q22.1-q22.33
 OMIM    176872
 3D Structure    ModBase 3D Structure for Q02750
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 Clonality    Polyclonal
 Host    Rabbit
 Species Reactivity    Recombinant
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 Specificity    Specific for the ~45k MEK 1 protein phosphorylated at Thr292 in Western blots.
 Purity/Purification    Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.)
 Form/Format    100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
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 Antigen    Phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr292 of human MEK 1.
 Immunogen Information    Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr292 conjugated to KLH
 Immunogen Species    Human
 Reactivity Assumed Based on 100% Sequence Homology    Bovine, canine, human, mouse, non-human primates and rat
 Species Reactivity Note    The antibody has been directly tested for reactivity in Western blots with recombinant MEK1. It is anticipated that the antibody will react with bovine, canine, human, mouse, non-human primates and rat based on the fact that these species have 100% homology with the amino acid sequence used as antigen.
 Biological Significance    MEK 1 (MAP kinase kinase, also known as MKK) is an integral component of the MAP kinase cascade that regulates cell growth and differentiation (Ahn, 1993; Chong et al., 2003). This pathway also plays a key role in synaptic plasticity in the brain (Adams and Sweatt, 2002). Activated MEK 1 acts as a dual specificity kinase phosphorylating both a threonine and a tyrosine residue on MAP kinase (Kyriakis et al., 1991; Seger et al., 1991; Crews et al., 1992). Conversely, there also appears to be a feedback phosphorylation of MEK 1 by MAP kinase. The sites on MEK 1 that are phosphorylated by MAP kinase are Thr292 and Thr386 (Mansour et al., 1994).
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 Preparation and Storage    For long term storage -20 degree C is recommended. Stable at -20 degree C for at least 1 year.
 Other Notes    Small volumes of anti-MAP2K1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-MAP2K1 antibody

   Affinity purified rabbit polyclonal antibody
 Applications Tested/Suitable for anti-MAP2K1 antibody   

Western Blot (WB)

 Application Notes for anti-MAP2K1 antibody    Quality Control: Western blots performed on each lot.
WB: 1:1000
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 Testing Data of anti-MAP2K1 antibody    anti-MAP2K1 antibody Testing Data image
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NCBI/Uniprot data below describe general gene information for MAP2K1. It may not necessarily be applicable to this product.
 NCBI GI #    5579478
 NCBI GeneID    5604
 NCBI Accession #    NP_002746.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_002755.3 [Other Products]
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 UniProt Primary Accession #    Q02750 [Other Products]
 UniProt Related Accession #    Q02750 [Other Products]
 Molecular Weight    45
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 NCBI Official Full Name    dual specificity mitogen-activated protein kinase kinase 1
 NCBI Official Synonym Full Names    mitogen-activated protein kinase kinase 1
 NCBI Official Symbol    MAP2K1 [Similar Products]
 NCBI Official Synonym Symbols   
[Similar Products]
 NCBI Protein Information    dual specificity mitogen-activated protein kinase kinase 1; MEK 1; MAPKK 1; MAPK/ERK kinase 1; ERK activator kinase 1; protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1)
 UniProt Protein Name    Dual specificity mitogen-activated protein kinase kinase 1
 UniProt Synonym Protein Names   
ERK activator kinase 1; MAPK/ERK kinase 1
 UniProt Gene Name    MAP2K1 [Similar Products]
 UniProt Synonym Gene Names    MEK1; PRKMK1; MAP kinase kinase 1; MAPKK 1; MKK1; MEK 1 [Similar Products]
 UniProt Entry Name    MP2K1_HUMAN
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 NCBI Summary for MAP2K1    The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. [provided by RefSeq, Jul 2008]
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 UniProt Comments for MAP2K1    Function: Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Binding of extracellular ligands such as growth factors, cytokines and hormones to their cell-surface receptors activates RAS and this initiates RAF1 activation. RAF1 then further activates the dual-specificity protein kinases MAP2K1/MEK1 and MAP2K2/MEK2. Both MAP2K1/MEK1 and MAP2K2/MEK2 function specifically in the MAPK/ERK cascade, and catalyze the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in the extracellular signal-regulated kinases MAPK3/ERK1 and MAPK1/ERK2, leading to their activation and further transduction of the signal within the MAPK/ERK cascade. Depending on the cellular context, this pathway mediates diverse biological functions such as cell growth, adhesion, survival and differentiation, predominantly through the regulation of transcription, metabolism and cytoskeletal rearrangements. One target of the MAPK/ERK cascade is peroxisome proliferator-activated receptor gamma (PPARG), a nuclear receptor that promotes differentiation and apoptosis. MAP2K1/MEK1 has been shown to export PPARG from the nucleus. The MAPK/ERK cascade is also involved in the regulation of endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC), as well as in the fragmentation of the Golgi apparatus during mitosis. Ref.6 Ref.9

Catalytic activity: ATP + a protein = ADP + a phosphoprotein.

Enzyme regulation: Ras proteins such as HRAS mediate the activation of RAF proteins such as RAF1 or BRAF which in turn activate extracellular signal-regulated kinases (ERK) through MAPK (mitogen-activated protein kinases) and ERK kinases MAP2K1/MEK1 and MAP2K2/MEK2. Activation occurs through phosphorylation of Ser-218 and Ser-222. MAP2K1/MEK1 is also the target of negative feed-back regulation by its substrate kinases, such as MAPK1/ERK2. These phosphorylate MAP2K1/MEK1 on Thr-292, thereby facilitating dephosphorylation of the activating residues Ser-218 and Ser-222. Inhibited by serine/threonine phosphatase 2A

By similarity. Many inhibitors have been identified including pyrrole derivatives, TAK-733 (one of a series of 8-methylpyrido[2,3-d]pyrimidine-4,7(3H,8H)-dione derivatives), CH4987655 and RDEA119/BAY 869766.

Subunit structure: Found in a complex with at least BRAF, HRAS, MAP2K1, MAPK3/ERK1 and RGS14

By similarity. Forms a heterodimer with MAP2K2/MEK2

By similarity. Forms heterodimers with KSR2 which further dimerize to form tetramers

By similarity. Interacts with ARBB2, LAMTOR3, MAPK1/ERK2, MORG1 and RAF1

By similarity. Interacts with PPARG and with isoform 1 of VRK2. Interacts with Yersinia yopJ. Interacts with SGK1. Interacts with BIRC6/bruce.

Subcellular location: Cytoplasm › cytoskeleton › microtubule organizing center › centrosome. Cytoplasm › cytoskeleton › microtubule organizing center › spindle pole body. Cytoplasm. Nucleus. Note: Localizes at centrosomes during prometaphase, midzone during anaphase and midbody during telophase/cytokinesis. Ref.6 Ref.9

Tissue specificity: Widely expressed, with extremely low levels in brain. Ref.1

Domain: The proline-rich region localized between residues 270 and 307 is important for binding to RAF1 and activation of MAP2K1/MEK1

By similarity.

Post-translational modification: Phosphorylation at Ser-218 and Ser-222 by MAP kinase kinase kinases (RAF or MEKK1) positively regulates kinase activity. Also phosphorylated at Thr-292 by MAPK1/ERK2 and at Ser-298 by PAK. MAPK1/ERK2 phosphorylation of Thr-292 occurs in response to cellular adhesion and leads to inhibition of Ser-298 phosphorylation by PAK. Ref.3 Ref.7Acetylation by Yersinia yopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway. Ref.8

Involvement in disease: Cardiofaciocutaneous syndrome 3 (CFC3) [MIM:615279]: A form of cardiofaciocutaneous syndrome, a multiple congenital anomaly disorder characterized by a distinctive facial appearance, heart defects and mental retardation. Heart defects include pulmonic stenosis, atrial septal defects and hypertrophic cardiomyopathy. Some affected individuals present with ectodermal abnormalities such as sparse, friable hair, hyperkeratotic skin lesions and a generalized ichthyosis-like condition. Typical facial features are similar to Noonan syndrome. They include high forehead with bitemporal constriction, hypoplastic supraorbital ridges, downslanting palpebral fissures, a depressed nasal bridge, and posteriorly angulated ears with prominent helices. Distinctive features of CFC3 include macrostomia and horizontal shape of palpebral fissures.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.29 Ref.30

Sequence similarities: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.Contains 1 protein kinase domain.
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Product References and Citations for anti-MAP2K1 antibody

   • Adams JP, Sweatt JD (2002) Molecular psychology: Roles for the ERK MAP kinase cascade in memory. Annu Rev Pharmacol Toxicol 42:135-163.
• Ahn NG (1993) The MAP kinase cascade. Discovery of a new signal transduction pathway. Mol Cell Biochem 127-128:201-209. Chong H, Vikis HG, Guan KL (2003) Mechanisms of regulating the Raf kinase family. Cellular Signalling 15:463-469. Crews CM, Alessandrini A, Erikson RL (1992) The primary structure of MEK, a protein kinase that phosphorylates the ERK gene product. Science 258:478-480. Kyriakis JM, Brautigan DL, Ingebritsen TS, Avruch J (1991) pp54 Microtubule-associated protein-2 kinase requires both tyrosine and serine/threonine phosphorylation for activity. J Biol Chem 266:10043-10046.
• Mansour SJ, Resing KA, Candi JM, Hermann AS, Gloor JW, Herskind KR, Wartmann M, Davis RJ, Ahn NG (1994) Mitogen-activated protein (MAP) kinase phosphorylation of MAP kinase kinase: Determination of phosphorylation sites by mass spectrometry and site-directed mutagenesis. J Biochem (Tokyo) 116:304-314.
• Seger R, Ahn NG, Boulton TG, Yancopoulos GD, Panayotatos N, Radziejewska E, Ericsson L, Bratlien RL, Cobb MH, Krebs EG (1991) Microtubule-associated protein 2 kinases, ERK1 and ERK2, undergo autophos-phorylation on both tyrosine and threonine residues: Implications for their mechanism of activation. Proc Natl Acad Sci USA 88:6142-6146.
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 Research Articles on MAP2K1    1. MEK1-YAP interaction is critical for liver cancer cell proliferation and maintenance of transformed phenotypes both in vitro and in vivo.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-MAP2K1 antibodyPathways associated with anti-MAP2K1 antibody
 Reference Product  PubMed Publications
 MAPK1 antibody  >390 publications with MAP2K1 and MAPK1
 MAPK3 antibody  >357 publications with MAP2K1 and MAPK3
 BRAF antibody  >102 publications with MAP2K1 and BRAF
 RAF1 antibody  >39 publications with MAP2K1 and RAF1
 MAP3K1 antibody  >28 publications with MAP2K1 and MAP3K1
 KSR1 antibody  >3 publications with MAP2K1 and KSR1
 IL2 antibody  >1 publications with MAP2K1 and IL2
 YWHAE antibody  >1 publications with MAP2K1 and YWHAE
 Products by Pathway  Pathway Diagram
 AGE/RAGE Pathway antibodies  AGE/RAGE Pathway Diagram
 ARMS-mediated Activation Pathway antibodies  ARMS-mediated Activation Pathway Diagram
 Activated TLR4 Signalling Pathway antibodies  Activated TLR4 Signalling Pathway Diagram
 Acute Myeloid Leukemia Pathway antibodies  Acute Myeloid Leukemia Pathway Diagram
 Acute Myeloid Leukemia Pathway antibodies  Acute Myeloid Leukemia Pathway Diagram
 Alcoholism Pathway antibodies  Alcoholism Pathway Diagram
 Alcoholism Pathway antibodies  Alcoholism Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 B Cell Receptor Signaling Pathway antibodies  B Cell Receptor Signaling Pathway Diagram
 B Cell Receptor Signaling Pathway antibodies  B Cell Receptor Signaling Pathway Diagram
Diseases associated with anti-MAP2K1 antibodyOrgans/Tissues associated with anti-MAP2K1 antibody
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >411 publications with MAP2K1 and Neoplasms
 Necrosis Antibodies  >96 publications with MAP2K1 and Necrosis
 Cardiovascular Diseases Antibodies  >71 publications with MAP2K1 and Cardiovascular Diseases
 Inflammation Antibodies  >60 publications with MAP2K1 and Inflammation
 Breast Neoplasms Antibodies  >52 publications with MAP2K1 and Breast Neoplasms
 Melanoma Antibodies  >45 publications with MAP2K1 and Melanoma
 Heart Diseases Antibodies  >44 publications with MAP2K1 and Heart Diseases
 Lung Neoplasms Antibodies  >35 publications with MAP2K1 and Lung Neoplasms
 Neoplasm Invasiveness Antibodies  >32 publications with MAP2K1 and Neoplasm Invasiveness
 Neoplasm Metastasis Antibodies  >25 publications with MAP2K1 and Neoplasm Metastasis
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >199 publications with MAP2K1 and Blood
 Vascular Antibodies  >87 publications with MAP2K1 and Vascular
 Lung Antibodies  >82 publications with MAP2K1 and Lung
 Muscle Antibodies  >80 publications with MAP2K1 and Muscle
 Brain Antibodies  >71 publications with MAP2K1 and Brain
 Heart Antibodies  >59 publications with MAP2K1 and Heart
 Skin Antibodies  >54 publications with MAP2K1 and Skin
 Bone Antibodies  >53 publications with MAP2K1 and Bone
 Kidney Antibodies  >47 publications with MAP2K1 and Kidney
 Nerve Antibodies  >42 publications with MAP2K1 and Nerve
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