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anti-MELK antibody :: MELK Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS8212103
Unit / Price
0.03 mL  /  $155 +1 FREE 8GB USB
0.1 mL  /  $220 +1 FREE 8GB USB
0.2 mL  /  $295 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

MELK, Polyclonal Antibody

★Popular Item★
 Also Known As   

Anti-MELK Antibody

 Product Synonym Names    KIAA0175; Maternal embryonic leucine zipper kinase; hMELK; Protein kinase Eg3; pEg3 kinase; Protein kinase PK38; hPK38; Tyrosine-protein kinase MELK
 Product Gene Name   

anti-MELK antibody

[Similar Products]
 Antibody/Peptide Pairs    MELK peptide (MBS8235449) is used for blocking the activity of MELK antibody (MBS8212103)
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    607025
 3D Structure    ModBase 3D Structure for Q14680
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 Clonality    Polyclonal
 Species Reactivity    Human, Mouse, Rat, Monkey
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 Specificity    Recognizes endogenous levels of MELK protein.
 Purity/Purification    The antibody was purified by immunogen affinity chromatography.
 Form/Format    Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
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 Source    Rabbit
 Immunogen    KLH-conjugated synthetic peptide encompassing a sequence within the center region of human MELK. The exact sequence is proprietary.
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 Preparation and Storage    Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
 Other Notes    Small volumes of anti-MELK antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-MELK antibody

   Rabbit polyclonal antibody to MELK
 Applications Tested/Suitable for anti-MELK antibody   

Western Blot (WB),Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

 Application Notes for anti-MELK antibody    WB (1/500 - 1/1000); IHC (1/100 - 1/200); IF (1/100 - 1/500); ICC (1/100 - 1/500)
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 Western Blot (WB) of anti-MELK antibody    Western blot analysis of MELK expression in MCF7 (A), NIH3T3 (B), mouse liver (C), rat liver (D) whole cell lysates.
anti-MELK antibody Western Blot (WB) (WB) image
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 Immunohistochemistry (IHC) of anti-MELK antibody    Immunohistochemical analysis of MELK staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
anti-MELK antibody Immunohistochemistry (IHC) (IHC) image
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 Immunofluorescence (IF) of anti-MELK antibody    Immunofluorescent analysis of MELK staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 degree C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
anti-MELK antibody Immunofluorescence (IF) image
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NCBI/Uniprot data below describe general gene information for MELK. It may not necessarily be applicable to this product.
 NCBI GI #    375493532
 NCBI GeneID    9833
 NCBI Accession #    NP_001243614.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001256685.1 [Other Products]
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 UniProt Primary Accession #    Q14680 [Other Products]
 UniProt Secondary Accession #    A6P3A7; A6P3A8; B1AMQ6; B7Z1E6; B7Z5M5; B7Z6Q7; B7Z6R8; B7Z6Y0; B7Z7Q1; D3DRP8; F5H0Y0 [Other Products]
 UniProt Related Accession #    Q14680 [Other Products]
 Molecular Weight    69,116 Da
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 NCBI Official Full Name    maternal embryonic leucine zipper kinase isoform 2
 NCBI Official Synonym Full Names    maternal embryonic leucine zipper kinase
 NCBI Official Symbol    MELK [Similar Products]
 NCBI Official Synonym Symbols   
HPK38
[Similar Products]
 NCBI Protein Information    maternal embryonic leucine zipper kinase
 UniProt Protein Name    Maternal embryonic leucine zipper kinase
 UniProt Synonym Protein Names   
Protein kinase Eg3; pEg3 kinase; Protein kinase PK38; hPK38; Tyrosine-protein kinase MELK (EC:2.7.10.2)
 Protein Family    Maternal embryonic leucine zipper kinase
 UniProt Gene Name    MELK [Similar Products]
 UniProt Synonym Gene Names    KIAA0175; hMELK; pEg3 kinase; hPK38 [Similar Products]
 UniProt Entry Name    MELK_HUMAN
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 UniProt Comments for MELK    MELK: Serine/threonine-protein kinase involved in various processes such as cell cycle regulation, self-renewal of stem cells, apoptosis and splicing regulation. Has a broad substrate specificity; phosphorylates BCL2L14, CDC25B, MAP3K5/ASK1 and ZNF622. Acts as an activator of apoptosis by phosphorylating and activating MAP3K5/ASK1. Acts as a regulator of cell cycle, notably by mediating phosphorylation of CDC25B, promoting localization of CDC25B to the centrosome and the spindle poles during mitosis. Plays a key role in cell proliferation and carcinogenesis. Required for proliferation of embryonic and postnatal multipotent neural progenitors. Phosphorylates and inhibits BCL2L14, possibly leading to affect mammary carcinogenesis by mediating inhibition of the pro-apoptotic function of BCL2L14. Also involved in the inhibition of spliceosome assembly during mitosis by phosphorylating ZNF622, thereby contributing to its redirection to the nucleus. May also play a role in primitive hematopoiesis. Monomer. Interacts with ZNF622 and PPP1R8. Up-regulated in many cancers cells. Up-regulated upon treatment with radiation or 5-fluorouracil (5-FU) in colorectal cancer cells, suggesting that it might be associated with increased resistance of colorectal cells against radiation and 5- FU. Down-regulated upon siomycin A, a thiazole antibiotic, treatment, leading to inhibit tumor growth in vivo. Expressed in placenta, kidney, thymus, testis, ovary and intestine. Activated by autophosphorylation of the T-loop at Thr-167 and Ser-171: in contrast to other members of the SNF1 subfamily, phosphorylation at Thr-167 is not mediated by STK11/LKB1 but via autophosphorylation instead. Inhibited by calcium-binding. Kinase activity is also regulated by reducing agents: dithiothreitol (DTT) or reduced glutathione are required for kinase activity in vitro; such dependence is however not due to the presence of disulfide bonds. Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.

Protein type: EC 2.7.10.2; RNA splicing; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; EC 2.7.11.1; Protein kinase, CAMK; CAMK group; CAMKL family; MELK subfamily

Chromosomal Location of Human Ortholog: 9p13.2

Cellular Component: membrane; plasma membrane; cell cortex

Molecular Function: protein serine/threonine kinase activity; protein binding; non-membrane spanning protein tyrosine kinase activity; calcium ion binding; lipid binding; ATP binding

Biological Process: cell proliferation; peptidyl-tyrosine phosphorylation; apoptosis; positive regulation of apoptosis; protein amino acid autophosphorylation; hemopoiesis; G2/M transition of mitotic cell cycle; induction of apoptosis by oxidative stress
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 Research Articles on MELK    1. insight has been brought by the discovery of a protein complex of FOXM1 with the mitotic kinase MELK in cancer stem cells in brain cancers, as this protein complex appears to be cancer-specific
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-MELK antibodyDiseases associated with anti-MELK antibody
 Reference Product  PubMed Publications
 FOXM1 antibody  >9 publications with MELK and FOXM1
 MYBL2 antibody  >3 publications with MELK and MYBL2
 PSMA1 antibody  >1 publications with MELK and PSMA1
 DBNL antibody  >1 publications with MELK and DBNL
 TP53 antibody  >1 publications with MELK and TP53
 PPP1R8 antibody  >1 publications with MELK and PPP1R8
 ZNF622 antibody  >1 publications with MELK and ZNF622
 Disease Name  Pubmed Publications
 Kidney Diseases Antibodies  >35 publications with MELK and Kidney Diseases
 Hypertension Antibodies  >16 publications with MELK and Hypertension
 Cardiovascular Diseases Antibodies  >16 publications with MELK and Cardiovascular Diseases
 Liver Diseases Antibodies  >10 publications with MELK and Liver Diseases
 Fibrosis Antibodies  >9 publications with MELK and Fibrosis
 Inflammation Antibodies  >8 publications with MELK and Inflammation
 Breast Neoplasms Antibodies  >7 publications with MELK and Breast Neoplasms
 Necrosis Antibodies  >7 publications with MELK and Necrosis
 Disease Models, Animal Antibodies  >6 publications with MELK and Disease Models, Animal
 Atrophy Antibodies  >6 publications with MELK and Atrophy
Organs/Tissues associated with anti-MELK antibody
 Organ/Tissue Name  Pubmed Publications
 Kidney Antibodies  >56 publications with MELK and Kidney
 Blood Antibodies  >35 publications with MELK and Blood
 Brain Antibodies  >25 publications with MELK and Brain
 Embryonic Tissue Antibodies  >18 publications with MELK and Embryonic Tissue
 Liver Antibodies  >15 publications with MELK and Liver
 Vascular Antibodies  >14 publications with MELK and Vascular
 Heart Antibodies  >11 publications with MELK and Heart
 Lung Antibodies  >9 publications with MELK and Lung
 Bone Antibodies  >7 publications with MELK and Bone
 Muscle Antibodies  >6 publications with MELK and Muscle
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