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anti-MTOR antibody :: Rabbit anti-Human MTOR Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS240649
Unit / Price
0.05 mg  /  $495 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

MTOR, Polyclonal Antibody

 Also Known As   

Rabbit Polyclonal to Human MTOR

 Product Synonym Names    Anti-MTOR Antibody (phospho-Ser2448) IHC-plus; MTOR; FRAP1; FRAP2; FRAP; Mammalian target of rapamycin; RAFT1; Rapamycin and FKBP12 target 1; RAPT1; Rapamycin target protein 1; Human MTOR
 Product Gene Name   

anti-MTOR antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    601231
 3D Structure    ModBase 3D Structure for P42345
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 Clonality    Polyclonal
 Host    Rabbit
 Species Reactivity    Human
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 Specificity    Amino acids 2440-2457 of human mTOR.
 Purity/Purification    Immunoaffinity Purified
 Form/Format    0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
 Concentration    1 mg/ml (lot specific)
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 Target Species    Human
 Immunogen Description    Synthetic peptide from human mTOR.
 Immunogen Type    Synthetic peptide
 Immunogen    MTOR antibody was raised against synthetic peptide from human mTOR.
 Antigen Modification    pSer2448
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 Preparation and Storage    Store vial at -20 degree C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20 degree C or below. Avoid cycles of freezing and thawing.
 Other Notes    Small volumes of anti-MTOR antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Product Description specifically for anti-MTOR antibody

   The mammalian Target of Rapamycin (TOR, also known as mTOR) is an evolutionarily conserved serine/threonine kinase that regulates cell growth and cell cycle through its ability to integrate signals from nutrient levels and growth factors. It was initially discovered as a kinase whose ability to stimulate T cell proliferation in response to IL-2 could be inhibited by the immunosuppressive drug rapamycin.
 Product Categories/Family for anti-MTOR antibody    Family: Protein Kinase
Subfamily: PI3/PI4
 Applications Tested/Suitable for anti-MTOR antibody   

Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)

 Application Notes for anti-MTOR antibody    ELISA (1:15000 - 1:65000), IHC-P (5 ug/ml), WB (1:500 - 1:2000)
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibo...
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 Immunohistochemistry (IHC) of anti-MTOR antibody    Anti-MTOR antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
anti-MTOR antibody Immunohistochemistry (IHC) (IHC) image
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 Western Blot (WB) of anti-MTOR antibody    Anti-mTOR pS2448 Antibody - Western Blot. Affinity Purified Anti-mTOR pS 2448 (Rabbit) is shown to detect a 250 kD band (indicated) corresponding to phosphorylated human mTOR present in a 293T whole cell lysates. Cells were serum-starved for 24 hours prior to harvest. ~20 ug of lysate was loaded per lane for SDS-PAGE. Untreated cells are shown in lane 1, whereas cells in lane 2 were treated with IGF-1 (100 ng/ml) for 20 min prior to harvest. Follow reaction of antibody with a 1:2000 dilution of HRP Goat-a-Rabbit IgG for visualization.
anti-MTOR antibody Western Blot (WB) (WB) image
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NCBI/Uniprot data below describe general gene information for MTOR. It may not necessarily be applicable to this product.
 NCBI GI #    4826730
 NCBI GeneID    2475
 NCBI Accession #    NP_004949.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_004958.3 [Other Products]
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 UniProt Primary Accession #    P42345 [Other Products]
 UniProt Secondary Accession #    Q4LE76; Q5TER1; Q6LE87; Q96QG3; Q9Y4I3 [Other Products]
 UniProt Related Accession #    P42345 [Other Products]
 Molecular Weight    288,892 Da
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 NCBI Official Full Name    serine/threonine-protein kinase mTOR
 NCBI Official Synonym Full Names    mechanistic target of rapamycin (serine/threonine kinase)
 NCBI Official Symbol    MTOR [Similar Products]
 NCBI Official Synonym Symbols   
FRAP; FRAP1; FRAP2; RAFT1; RAPT1
[Similar Products]
 NCBI Protein Information    serine/threonine-protein kinase mTOR; rapamycin target protein 1; mammalian target of rapamycin; rapamycin and FKBP12 target 1; FKBP-rapamycin associated protein; rapamycin associated protein FRAP2; FKBP12-rapamycin complex-associated protein 1; FK506 binding protein 12-rapamycin associated protein 2; FK506-binding protein 12-rapamycin complex-associated protein 1
 UniProt Protein Name    Serine/threonine-protein kinase mTOR
 UniProt Synonym Protein Names   
FK506-binding protein 12-rapamycin complex-associated protein 1; FKBP12-rapamycin complex-associated protein; Mammalian target of rapamycin; mTOR; Mechanistic target of rapamycin; Rapamycin and FKBP12 target 1; Rapamycin target protein 1
 Protein Family    Serine/threonine-protein kinase
 UniProt Gene Name    MTOR [Similar Products]
 UniProt Synonym Gene Names    FRAP; FRAP1; FRAP2; RAFT1; RAPT1; mTOR [Similar Products]
 UniProt Entry Name    MTOR_HUMAN
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 NCBI Summary for MTOR    The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of this gene. [provided by RefSeq, Sep 2008]
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 UniProt Comments for MTOR    mTOR: an atypical kinase belonging to the PIKK family of kinases. Is the catalytic subunit of two protein complexes, mTORC1 and mTORC2. mTORC1 activates S6K and inactivates 4E-BP1, up-regulating protein synthesis. mTORC1 contains Raptor, a positive regulatory subunit and scaffold for recruiting substrates, two negative regulators, PRAS40 and DEPTOR, and mLST8; it is a target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. mTORC2, a downstream effector of PI3K, is insensitive to rapamycin and activates Akt by phosphorylating a key activation site. mTORC2 contains regulatory subunits Rictor and mSIN1, PROTOR, mLST8, and the negative regulator DEPTOR. mTORC1 suppresses PI3K activity via a strong negative feedback loop that involves S6K1. Inhibiting mTORC1 ablates this negative feedback loop and potentiates PI3K signaling. Known inhibitors of mTOR include rapamycin, temsirolimus (CCI-779).

Protein type: EC 2.7.11.1; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Motility/polarity/chemotaxis; Autophagy; Protein kinase, atypical; ATYPICAL group; PIKK family; FRAP subfamily

Chromosomal Location of Human Ortholog: 1p36.2

Cellular Component: endoplasmic reticulum membrane; PML body; lysosome; lysosomal membrane; dendrite; endomembrane system; cytosol; nucleoplasm; Golgi membrane; mitochondrial outer membrane; cell soma; membrane; cytoplasm; TORC2 complex; phosphoinositide 3-kinase complex; nucleus

Molecular Function: protein dimerization activity; protein domain specific binding; protein serine/threonine kinase activity; protein binding; ribosome binding; phosphoprotein binding; drug binding; kinase activity; ATP binding

Biological Process: negative regulation of autophagy; regulation of myelination; TOR signaling pathway; nerve growth factor receptor signaling pathway; positive regulation of translation; protein amino acid autophosphorylation; regulation of glycogen biosynthetic process; negative regulation of cell size; signal transduction; protein amino acid phosphorylation; germ cell development; negative regulation of macroautophagy; cellular response to nutrient levels; positive regulation of stress fiber formation; regulation of carbohydrate utilization; response to stress; protein catabolic process; cell growth; response to nutrient; regulation of response to food; epidermal growth factor receptor signaling pathway; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; peptidyl-threonine phosphorylation; positive regulation of lipid biosynthetic process; response to amino acid stimulus; negative regulation of NFAT protein import into nucleus; double-strand break repair via homologous recombination; positive regulation of protein kinase B signaling cascade; positive regulation of peptidyl-tyrosine phosphorylation; peptidyl-serine phosphorylation; positive regulation of actin filament polymerization; regulation of actin cytoskeleton organization and biogenesis; T cell costimulation; ruffle organization and biogenesis; insulin receptor signaling pathway; regulation of fatty acid beta-oxidation; positive regulation of transcription from RNA polymerase III promoter; innate immune response; positive regulation of endothelial cell proliferation; positive regulation of protein amino acid phosphorylation; vascular endothelial growth factor receptor signaling pathway; regulation of protein kinase activity; phosphorylation; growth
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 Research Articles on MTOR    1. The positive rates of mTOR, eIF4E and 4EBPs in patients with lymph node metastasis were significantly higher than in patients without lymph node metastasis.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-MTOR antibodyPathways associated with anti-MTOR antibody
 Reference Product  PubMed Publications
 TSC2 antibody  >631 publications with MTOR and TSC2
 EIF4EBP1 antibody  >528 publications with MTOR and EIF4EBP1
 AKT1 antibody  >430 publications with MTOR and AKT1
 RICTOR antibody  >345 publications with MTOR and RICTOR
 RHEB antibody  >291 publications with MTOR and RHEB
 RPTOR antibody  >177 publications with MTOR and RPTOR
 MLST8 antibody  >46 publications with MTOR and MLST8
 RPS6KB1 antibody  >38 publications with MTOR and RPS6KB1
 MAPKAP1 antibody  >24 publications with MTOR and MAPKAP1
 FKBP1A antibody  >6 publications with MTOR and FKBP1A
 Products by Pathway  Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 Acute Myeloid Leukemia Pathway antibodies  Acute Myeloid Leukemia Pathway Diagram
 Acute Myeloid Leukemia Pathway antibodies  Acute Myeloid Leukemia Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Adipocytokine Signaling Pathway antibodies  Adipocytokine Signaling Pathway Diagram
 Adipocytokine Signaling Pathway antibodies  Adipocytokine Signaling Pathway Diagram
 Alpha6-Beta4 Integrin Signaling Pathway antibodies  Alpha6-Beta4 Integrin Signaling Pathway Diagram
 BDNF Signaling Pathway antibodies  BDNF Signaling Pathway Diagram
Diseases associated with anti-MTOR antibodyOrgans/Tissues associated with anti-MTOR antibody
 Disease Name  Pubmed Publications
 Adenocarcinoma Antibodies  >1117 publications with MTOR and Adenocarcinoma
 Kidney Diseases Antibodies  >891 publications with MTOR and Kidney Diseases
 Disease Models, Animal Antibodies  >836 publications with MTOR and Disease Models, Animal
 Breast Neoplasms Antibodies  >753 publications with MTOR and Breast Neoplasms
 Carcinoma, Renal Cell Antibodies  >669 publications with MTOR and Carcinoma, Renal Cell
 Cardiovascular Diseases Antibodies  >599 publications with MTOR and Cardiovascular Diseases
 Neoplasms, Experimental Antibodies  >517 publications with MTOR and Neoplasms, Experimental
 Inflammation Antibodies  >517 publications with MTOR and Inflammation
 Lung Neoplasms Antibodies  >504 publications with MTOR and Lung Neoplasms
 Liver Diseases Antibodies  >464 publications with MTOR and Liver Diseases
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >1759 publications with MTOR and Blood
 Muscle Antibodies  >1416 publications with MTOR and Muscle
 Kidney Antibodies  >1376 publications with MTOR and Kidney
 Brain Antibodies  >1314 publications with MTOR and Brain
 Vascular Antibodies  >1293 publications with MTOR and Vascular
 Liver Antibodies  >1025 publications with MTOR and Liver
 Lung Antibodies  >874 publications with MTOR and Lung
 Heart Antibodies  >616 publications with MTOR and Heart
 Bone Antibodies  >467 publications with MTOR and Bone
 Prostate Antibodies  >416 publications with MTOR and Prostate
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