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| Product Name
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Macrophage Inflammatory Protein 5 (MIP5), Polyclonal Antibody ★Popular Item★
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| Also Known As
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Polyclonal Antibody to Macrophage Inflammatory Protein 5 (MIP5)
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| Product Gene Name
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[Similar Products]
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| Research Use Only
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For Research Use Only. Not for use in diagnostic procedures.
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 TOP
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| Immunogen Sequence
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The target protein is fused with N-terminal His-Tag and its sequence is listed below.
MGHHHHHHSG SEF- QFINDAETE LMMSKLPLEN PVVLNSFHFA ADCCTSYISQ SIPCSLMKSY FETSSECSKP GVIFLTKKGR QVCAKPSGPG VQDCMKKLKP YSI
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| OMIM
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U58914 mRNA
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| 3D Structure
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ModBase 3D Structure for Q16663
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| Clonality
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Polyclonal
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| Isotype
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IgG
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| Host
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Rabbit
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| Species Reactivity
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Human
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| Specificity
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The antibody is a rabbit polyclonal antibody raised against MIP5. It has been selected for its ability to recognize MIP5 in immunohistochemical staining andwestern blotting.
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| Purity/Purification
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Affinity Chromatography
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| Form/Format
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Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
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| Concentration
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200ug/ml (lot specific)
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| Immunogen
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Recombinant CCL15 (Gln22~Ile113) expressed in E.coli.
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| Conjugated Antibody
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The APC conjugated antibody version of this item is also available as catalog #MBS2043857
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| Preparation and Storage
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Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
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| Other Notes
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Small volumes of anti-MIP5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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TOP
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| Applications Tested/Suitable for anti-MIP5 antibody
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Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
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| Application Notes for anti-MIP5 antibody
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Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
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| Western Blot (WB) of anti-MIP5 antibody
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(Figure. Western Blot; Sample: Recombinant protein.)
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| Immunohistochemistry (IHC) of anti-MIP5 antibody
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(Figure.DAB staining on IHC-P. Samples: Human Tissue)
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TOP
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| NCBI/Uniprot data below describe general gene information for MIP5. It may not necessarily be applicable to this product. |
| NCBI GI #
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597709818
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| NCBI GeneID
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6359
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| NCBI Accession #
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NP_116741.2
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[Other Products]
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| NCBI GenBank Nucleotide #
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NM_032965.5
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[Other Products]
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| UniProt Primary Accession #
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Q16663
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[Other Products]
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| UniProt Secondary Accession #
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Q9UM74; B2RU34; E1P651
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[Other Products]
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| UniProt Related Accession #
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Q16663
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[Other Products]
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| Molecular Weight
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12,248 Da
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TOP
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| NCBI Official Full Name
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C-C motif chemokine 15 preproprotein
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| NCBI Official Synonym Full Names
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C-C motif chemokine ligand 15
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| NCBI Official Symbol
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CCL15
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[Similar Products]
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| NCBI Official Synonym Symbols
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LKN1; NCC3; SY15; HCC-2; LKN-1; MIP-5; NCC-3; SCYL3; MIP-1D; MRP-2B; SCYA15; HMRP-2B; MIP-1 delta
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[Similar Products]
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| NCBI Protein Information
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C-C motif chemokine 15
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| UniProt Protein Name
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C-C motif chemokine 15
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| UniProt Synonym Protein Names
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Chemokine CC-2; HCC-2
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| UniProt Gene Name
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CCL15
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[Similar Products]
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| UniProt Synonym Gene Names
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MIP5; NCC3; SCYA15; HCC-2; LKN-1; MIP-5
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[Similar Products]
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| NCBI Summary for MIP5
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This gene is located in a cluster of similar genes in the same region of chromosome 17. These genes encode CC cytokines, which are secreted proteins characterized by two adjacent cysteines. The product of this gene is chemotactic for T cells and monocytes, and acts through C-C chemokine receptor type 1 (CCR1). The proprotein is further processed into numerous smaller functional peptides. Naturally-occurring readthrough transcripts occur from this gene into the downstream gene, CCL14 (chemokine (C-C motif) ligand 14). [provided by RefSeq, Jan 2013]
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| UniProt Comments for MIP5
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CCL15: Chemotactic factor that attracts T-cells and monocytes, but not neutrophils, eosinophils, or B-cells. Acts mainly via CC chemokine receptor CCR1. Also binds to CCR3. CCL15(22-92), CCL15(25-92) and CCL15(29-92) are more potent chemoattractants than the small-inducible cytokine A15. Belongs to the intercrine beta (chemokine CC) family.
Protein type: Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 17q12
Cellular Component: cell; extracellular space
Molecular Function: CCR chemokine receptor binding; chemoattractant activity; chemokine activity; heparin binding; receptor binding
Biological Process: cell-cell signaling; cellular calcium ion homeostasis; chemotaxis; G-protein coupled receptor signaling pathway; inflammatory response; lymphocyte chemotaxis; monocyte chemotaxis; neutrophil chemotaxis; positive chemotaxis; positive regulation of GTPase activity; signal transduction
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TOP
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| Research Articles on MIP5
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1. CCL15 activation of CCR1 plays critical roles in hepatocellular carcinoma metastasis.
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| Precautions
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All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
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| Disclaimer
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While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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| Products associated with anti-MIP5 antibody | Pathways associated with anti-MIP5 antibody |
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| Diseases associated with anti-MIP5 antibody | Organs/Tissues associated with anti-MIP5 antibody |
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Antibody 
