Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource rightarrow Antibody rightarrow MECP2 rightarrow Polyclonal MECP2  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Menu separator
separator
ELISA Kit ELISA Kit
CLIA Kit CLIA Kit
PCR Kit PCR Kit
Monoclonal Antibody Monoclonal Antibody
Polyclonal Antibody Polyclonal Antibody
Secondary Antibody Secondary Antibody
Antigen Antigen
Biochemical Biochemical
cDNA Clone cDNA Clone
siRNA siRNA
Peptide Peptide
Recombinant/Purified Protein Rec./Purified Protein

Custom ELISA Kit Custom ELISA Kit
Custom Protein Custom Protein
Custom Antibody Custom Antibody
Antibody Matched Pairs Antibody Matched Pairs
Antibody & Corresponding Blocking Peptide Pairs Antibody Peptide Pairs
Phospho-Specific Antibodies Phospho Antibodies
Products by Disease Products by Disease
Products by Pathway Products by Pathway
Products by Tissue Products by Tissue

arrow Advanced Search
arrow Submit Technical Q&A
arrow International Distributors
arrow Contact Us
separator
Our Best Sellers moreseparator
separator
 • Soluble Cytotoxic T-Lymphocyte Associated Antigen 4 (SCTLA4) ELISA Kit
 • PARL Antibody
 • pentosidine, PE ELISA Kit
 • Free Thyroxine (FT4) ELISA Kit
 • tuberculosis (TB) antibody (IgG) ELISA Kit
 • Extracellular calcium-sensing receptor (Casr) Recombinant Protein
 • phosphorylated microtubule Associated protein tau (pMAPT-pTA... ELISA Kit
 • Ubiquitin ELISA Kit
 • RNF10 Antibody
 • Complement C7 Native Protein
 • oculocerebrorenal syndrome of Lowe (OCRL) Antibody
 • c-myc Antibody
 • engrailed homeobox 2 (EN2) ELISA Kit
 • trypsinogen activation peptide, TAP ELISA Kit
 downarrow more ...
separator
separator
DatasheetFull DatasheetPrinter Friendly DatasheetPrint This DatasheetAdd to Compare ListHave Questions? Ask UsRequest for a Quotation today

anti-MECP2 antibody :: Rabbit MeCP2 (Ser80) Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS502151
Testing Data
Unit / Price
0.1 mL  /  $315 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

MeCP2 (Ser80), Polyclonal Antibody

 Also Known As   

Anti-Phospho-Ser80 MECP2

 Product Gene Name   

anti-MECP2 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
Table BarTOPTable Bar
 Chromosome Location    Chromosome: X; NC_000023.11 (154021800..154097731, complement). Location: Xq28
 OMIM    105830
 3D Structure    ModBase 3D Structure for P51608
Section Bar
 Clonality    Polyclonal
 Host    Rabbit
 Species Reactivity    Human, mouse, rat
Section Bar
 Specificity    Specific for the ~75 kDa MECP2 protein phosphorylated at Ser80 in Western blots of human, rat and mouse brain extracts. The antibody has also been used successfully for immunohistochemistry on mouse brain sections.
 Purity/Purification    Serum
 Form/Format    100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol.. Adequate amount of material to conduct 10-mini Western Blots
Section Bar
 Antigen    Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser80 of MECP2 (Methyl-CpG Binding Protein 2) protein.
 Immunogen Information    Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser80 conjugated to KLH
 Immunogen Species    Human
 Species Reactivity Note    The antibody has been directly tested for reactivity in human, mouse and rat. It has not been tested for reactivity with other species.
 Biological Significance    MECP2 (Methyl-CpG Binding Protein 2) is a chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair and is not influenced by sequences flanking the methyl-CpGs. MECP2 has been shown to mediate transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A. Defects in MECP2 are the cause of Rett syndrome (RTT). RTT is an X-linked dominant disease, it is a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females. Recent studies have reported a new phosphorylation site at Ser80. Phosphorylation and dephosphorylation of this site may be involved in modulating the dynamic function of MECP2 in neurons transiting between resting and active states within neural circuits that underlie behaviors. (Tao et al., 2009)
Section Bar
 Preparation and Storage    Store at -20 degree C in undiluted aliquots; stable for at least 1 year after date of receipt. Avoid freeze/thaw cycles.
 Other Notes    Small volumes of anti-MECP2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Table BarTOPTable Bar
 

Related Product Information for anti-MECP2 antibody

   Rabbit polyclonal antibody
 Applications Tested/Suitable for anti-MECP2 antibody   

Western Blot (WB), Immunohistochemistry (IHC)

 Application Notes for anti-MECP2 antibody    Quality Control: Western blots performed on each lot.
WB: 1:1,000
IHC 1:100
Section Bar
 Testing Data of anti-MECP2 antibody    anti-MECP2 antibody Testing Data image
Table BarTOPTable Bar
NCBI/Uniprot data below describe general gene information for MECP2. It may not necessarily be applicable to this product.
 NCBI GI #    160707950
 NCBI GeneID    4204
 NCBI Accession #    NP_001104262.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001110792.1 [Other Products]
Section Bar
 UniProt Primary Accession #    P51608 [Other Products]
 UniProt Secondary Accession #    O15233; Q6QHH9; Q7Z384 [Other Products]
 UniProt Related Accession #    P51608 [Other Products]
 Molecular Weight    75
Table BarTOPTable Bar
 NCBI Official Full Name    methyl-CpG-binding protein 2 isoform 2
 NCBI Official Synonym Full Names    methyl CpG binding protein 2 (Rett syndrome)
 NCBI Official Symbol    MECP2 [Similar Products]
 NCBI Official Synonym Symbols   
RS; RTS; RTT; PPMX; MRX16; MRX79; MRXSL; AUTSX3; MRXS13
[Similar Products]
 NCBI Protein Information    methyl-CpG-binding protein 2; meCp-2 protein
 UniProt Protein Name    Methyl-CpG-binding protein 2
 Protein Family    Methyl-CpG-binding protein
 UniProt Gene Name    MECP2 [Similar Products]
 UniProt Synonym Gene Names    MeCp-2 protein; MeCp2 [Similar Products]
 UniProt Entry Name    MECP2_HUMAN
Section Bar
 NCBI Summary for MECP2    DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. In contrast to other MBD family members, MECP2 is X-linked and subject to X inactivation. MECP2 is dispensible in stem cells, but is essential for embryonic development. MECP2 gene mutations are the cause of most cases of Rett syndrome, a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females. [provided by RefSeq, Jul 2009]
Section Bar
 UniProt Comments for MECP2    Function: Chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. Mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A. Binds both 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC)-containing DNA, with a preference for 5-methylcytosine (5mC)

By similarity.

Subunit structure: Interacts with FNBP3

By similarity. Interacts with CDKL5. Ref.14

Subcellular location: Nucleus. Note: Colocalized with methyl-CpG in the genome.

Tissue specificity: Present in all adult somatic tissues tested.

Post-translational modification: Phosphorylated on Ser-423 in brain upon synaptic activity, which attenuates its repressor activity and seems to regulate dendritic growth and spine maturation

By similarity.

Involvement in disease: Angelman syndrome (AS) [MIM:105830]: A neurodevelopmental disorder characterized by severe motor and intellectual retardation, ataxia, frequent jerky limb movements and flapping of the arms and hands, hypotonia, seizures, absence of speech, frequent smiling and episodes of paroxysmal laughter, open-mouthed expression revealing the tongue.Note: The disease may be caused by mutations affecting the gene represented in this entry. Ref.36 Ref.40Mental retardation, X-linked, syndromic, 13 (MRXS13) [MIM:300055]: A disorder characterized by significantly below average general intellectual functioning associated with impairments in adaptive behavior and manifested during the developmental period. MRXS13 patients manifest mental retardation associated with other variable features such as spasticity, episodes of manic depressive psychosis, increased tone and macroorchidism.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.26 Ref.28 Ref.38 Ref.44 Ref.48 Ref.49 Ref.50 Ref.54 Ref.58Rett syndrome (RTT) [MIM:312750]: An X-linked dominant neurodevelopmental disorder, and one of the most common causes of mental retardation in females. Patients appear to develop normally until 6 to 18 months of age, then gradually lose speech and purposeful hand movements, and develop microcephaly, seizures, autism, ataxia, mental retardation and stereotypic hand movements. After initial regression, the condition stabilizes and patients usually survive into adulthood.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.5 Ref.24 Ref.25 Ref.27 Ref.29 Ref.30 Ref.31 Ref.32 Ref.33 Ref.34 Ref.35 Ref.36 Ref.37 Ref.39 Ref.40 Ref.42 Ref.43 Ref.49 Ref.51 Ref.52 Ref.53 Ref.56Autism, X-linked 3 (AUTSX3) [MIM:300496]: A complex multifactorial, pervasive developmental disorder characterized by impairments in reciprocal social interaction and communication, restricted and stereotyped patterns of interests and activities, and the presence of developmental abnormalities by 3 years of age. Most individuals with autism also manifest moderate mental retardation.Note: The disease may be caused by mutations affecting the gene represented in this entry. Ref.55Encephalopathy, neonatal severe, due to MECP2 mutations (ENS-MECP2) [MIM:300673]: A neurodevelopmental disorder characterized by severe neonatal encephalopathy, developmental delay, mental retardation, microcephaly, seizures. Additional features include respiratory insufficiency and central hypoventilation, gastroesophageal reflux, axial hypotonia, hyperreflexia and dyskinetic movements.Note: The disease is caused by mutations affecting the gene represented in this entry. The MECP2 gene is mutated in Rett syndrome, a severe neurodevelopmental disorder that almost always occurs in females. Although it was first thought that MECP2 mutations causing Rett syndrome were lethal in males, later reports identified a severe neonatal encephalopathy in surviving male sibs of patients with Rett syndrome. Additional reports have confirmed a severe phenotype in males with Rett syndrome-associated MECP2 mutations. Ref.41Mental retardation, X-linked, syndromic, Lubs type (MRXSL) [MIM:300260]: A disorder characterized by significantly below average general intellectual functioning associated with impairments in adaptive behavior and manifested during the developmental period. MRXSL patients manifest mental retardation associated with variable features. They include swallowing dysfunction and gastroesophageal reflux with secondary recurrent respiratory infections, hypotonia, mild myopathy and characteristic facies such as downslanting palpebral fissures, hypertelorism and a short nose with a low nasal bridge.Note: The disease is caused by mutations affecting the gene represented in this entry. Increased dosage of MECP2 due to gene duplication appears to be responsible for the mental retardation phenotype. Ref.57

Sequence similarities: Contains 2 A.T hook DNA-binding domains.Contains 1 MBD (methyl-CpG-binding) domain.

Sequence caution: The sequence CAD97991.1 differs from that shown. Reason: Erroneous initiation.
Table BarTOPTable Bar
 

Product References and Citations for anti-MECP2 antibody

   • Tao J, Hu K, Chang Q, Wu H, Sherman NE, Martinowich K, Klose RJ, Schanen C, Jaenisch R, Wang W, Sun YE (2009) Phosphorylation of MeCP2 at Serine 80 regulates its chromatin association and neurological function. Proc Natl Acad Sci U S A.106(12):4882-7.
Section Bar
 Research Articles on MECP2    1. The presence of multiple MECP2 mutations was not associated with greater severity in Rett syndrome patients.
Table BarTOPTable Bar
 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Table BarTOPTable Bar
Products associated with anti-MECP2 antibodyPathways associated with anti-MECP2 antibody
 Reference Product  PubMed Publications
 BDNF antibody  >111 publications with MECP2 and BDNF
 DNMT1 antibody  >94 publications with MECP2 and DNMT1
 CDKL5 antibody  >71 publications with MECP2 and CDKL5
 HDAC1 antibody  >43 publications with MECP2 and HDAC1
 SIN3A antibody  >27 publications with MECP2 and SIN3A
 ATRX antibody  >15 publications with MECP2 and ATRX
 YY1 antibody  >1 publications with MECP2 and YY1
 Products by Pathway  Pathway Diagram
 SIDS Susceptibility Pathways antibodies  SIDS Susceptibility Pathways Diagram
Diseases associated with anti-MECP2 antibodyOrgans/Tissues associated with anti-MECP2 antibody
 Disease Name  Pubmed Publications
 Rett Syndrome Antibodies  >1099 publications with MECP2 and Rett Syndrome
 Intellectual Disability Antibodies  >967 publications with MECP2 and Intellectual Disability
 Disease Models, Animal Antibodies  >205 publications with MECP2 and Disease Models, Animal
 Brain Diseases Antibodies  >152 publications with MECP2 and Brain Diseases
 Seizures Antibodies  >135 publications with MECP2 and Seizures
 Autistic Disorder Antibodies  >122 publications with MECP2 and Autistic Disorder
 Child Development Disorders, Pervasive Antibodies  >86 publications with MECP2 and Child Development Disorders, Pervasive
 Chromosome Aberrations Antibodies  >73 publications with MECP2 and Chromosome Aberrations
 Angelman Syndrome Antibodies  >52 publications with MECP2 and Angelman Syndrome
 Developmental Disabilities Antibodies  >37 publications with MECP2 and Developmental Disabilities
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >674 publications with MECP2 and Brain
 Blood Antibodies  >127 publications with MECP2 and Blood
 Muscle Antibodies  >49 publications with MECP2 and Muscle
 Liver Antibodies  >39 publications with MECP2 and Liver
 Embryonic Tissue Antibodies  >31 publications with MECP2 and Embryonic Tissue
 Bone Antibodies  >23 publications with MECP2 and Bone
 Lung Antibodies  >23 publications with MECP2 and Lung
 Eye Antibodies  >22 publications with MECP2 and Eye
 Prostate Antibodies  >15 publications with MECP2 and Prostate
 Ganglia Antibodies  >14 publications with MECP2 and Ganglia
Table BarTOPTable Bar
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions