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anti-PMEL antibody :: Rabbit anti-Human Melanoma Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS301393
Immunohistochemistry (IHC)
Unit / Price
  DISCONTINUED
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 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Melanoma (PMEL), Polyclonal Antibody

★Popular Item★
 Also Known As   

Rabbit Anti-Human Melanoma (GP100) Polyclonal Antibody

 Product Gene Name   

anti-PMEL antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    155550
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 Clonality    Polyclonal
 Isotype    IgG
 Host    Rabbit
 Species Reactivity    Human
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 Form/Format    0.1 ml immunogen affinity purified rabbit polyclonal antibody in PBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide.
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 Immunogen    Recombinant protein encoding aa 1-150 human gp100.
 Control Tissue    Melanoma
 Cellular Localization    Cytoplasm
 Entrez Gene Code    6490
 Pathway    Cancer Markers
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 Preparation and Storage    Store at 2-8 degree C. Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product; therefore, positive and negative controls should be tested simultaneously with unknown specimens. If unexpected results are observed which cannot be explained by variations in laboratory procedures and a problem with the reagent is suspected, contact Technical Support.
 Other Notes    Small volumes of anti-PMEL antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-PMEL antibody

   By immunohistochemistry, this antibody specifically recognizes a protein in melanocytes and melanomas. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.
 Applications Tested/Suitable for anti-PMEL antibody   

Immunohistochemistry (IHC) Paraffin

 Application Notes for anti-PMEL antibody    Immunohistochemistry Procedure
Specimen Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody. Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols. Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:50. The dilutions are estimates; actual results may differ because of variability in methods and protocols. Antigen Retrieval: Boil tissue section in 10mM Citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. Primary Antibody Incubation: Incubate for 10 minutes at RT. Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween. Visualization: Detect the antibody as instructed by the instructions provided with the visualization system.
Immunohistochemistry Dilution: 1:50
IHC Positive Control: Melanoma
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 Immunohistochemistry (IHC) of anti-PMEL antibody    Human melanoma stained with anti-melanoma gp100 antibody
anti-PMEL antibody Immunohistochemistry (IHC) (IHC) image
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NCBI/Uniprot data below describe general gene information for PMEL. It may not necessarily be applicable to this product.
 NCBI GI #    318037593
 NCBI GeneID    6490
 NCBI Accession #    NP_001186982.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001200053.1 [Other Products]
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 UniProt Secondary Accession #    Q12763; Q14448; Q14817; Q16565; B3KS57; B7Z6D7 [Other Products]
 UniProt Related Accession #    P40967 [Other Products]
 Molecular Weight    100 kDa [Similar Products]
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 NCBI Official Full Name    melanocyte protein PMEL isoform 2
 NCBI Official Synonym Full Names    premelanosome protein
 NCBI Official Symbol    PMEL [Similar Products]
 NCBI Official Synonym Symbols   
P1; SI; SIL; ME20; P100; SILV; ME20M; gp100; ME20-M; PMEL17; D12S53E
[Similar Products]
 NCBI Protein Information    melanocyte protein PMEL; melanocyte protein Pmel 17; melanocyte protein mel 17; melanocytes lineage-specific antigen GP100; melanoma-associated ME20 antigen; melanosomal matrix protein17; silver locus protein homolog; silver, mouse, homolog of
 UniProt Protein Name    Melanocyte protein PMEL
 UniProt Synonym Protein Names   
ME20-M; ME20M; Melanocyte protein Pmel 17; Melanocytes lineage-specific antigen GP100; Melanoma-associated ME20 antigen; P1; P100; Premelanosome protein; Silver locus protein homologCleaved into the following 2 chains:M-alphaAlternative name(s):95 kDa melanocyte-specific secreted glycoprotein; P26; Secreted melanoma-associated ME20 antigen; ME20-S; ME20S
 Protein Family    Melanoma antigen
 UniProt Gene Name    PMEL [Similar Products]
 UniProt Synonym Gene Names    D12S53E; PMEL17; SILV; ME20M; ME20-S; ME20S [Similar Products]
 UniProt Entry Name    PMEL_HUMAN
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 NCBI Summary for PMEL    This gene encodes a melanocyte-specific type I transmembrane glycoprotein. The encoded protein is enriched in melanosomes, which are the melanin-producing organelles in melanocytes, and plays an essential role in the structural organization of premelanosomes. This protein is involved in generating internal matrix fibers that define the transition from Stage I to Stage II melanosomes. This protein undergoes a complex pattern of prosttranslational processing and modification that is essential to the proper functioning of the protein. A secreted form of this protein that is released by proteolytic ectodomain shedding may be used as a melanoma-specific serum marker. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2011]
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 UniProt Comments for PMEL    SILV: Plays a central role in the biogenesis of melanosomes. Involved in the maturation of melanosomes from stage I to II. The transition from stage I melanosomes to stage II melanosomes involves an elongation of the vesicle, and the appearance within of distinct fibrillar structures. Release of the soluble form, ME20-S, could protect tumor cells from antibody mediated immunity. Belongs to the PMEL/NMB family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Vesicle

Chromosomal Location of Human Ortholog: 12q13-q14

Cellular Component: Golgi apparatus; endoplasmic reticulum membrane; plasma membrane; melanosome; integral to membrane; extracellular region

Molecular Function: protein binding

Biological Process: melanin biosynthetic process; melanosome organization and biogenesis
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 Research Articles on PMEL    1. Melanosome-autonomous regulation of size and number: the OA1 receptor sustains PMEL expression.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Diseases associated with anti-PMEL antibodyOrgans/Tissues associated with anti-PMEL antibody
 Disease Name  Pubmed Publications
 Skin Diseases Antibodies  >115 publications with PMEL and Skin Diseases
 Disease Models, Animal Antibodies  >81 publications with PMEL and Disease Models, Animal
 Necrosis Antibodies  >17 publications with PMEL and Necrosis
 Lung Diseases Antibodies  >15 publications with PMEL and Lung Diseases
 Brain Diseases Antibodies  >9 publications with PMEL and Brain Diseases
 Leukopenia Antibodies  >6 publications with PMEL and Leukopenia
 Neutropenia Antibodies  >6 publications with PMEL and Neutropenia
 Pain Antibodies  >5 publications with PMEL and Pain
 Kidney Diseases Antibodies  >5 publications with PMEL and Kidney Diseases
 Drug Hypersensitivity Antibodies  >4 publications with PMEL and Drug Hypersensitivity
 Organ/Tissue Name  Pubmed Publications
 Skin Antibodies  >127 publications with PMEL and Skin
 Lung Antibodies  >22 publications with PMEL and Lung
 Eye Antibodies  >21 publications with PMEL and Eye
 Spleen Antibodies  >19 publications with PMEL and Spleen
 Brain Antibodies  >16 publications with PMEL and Brain
 Kidney Antibodies  >12 publications with PMEL and Kidney
 Bone Antibodies  >10 publications with PMEL and Bone
 Testis Antibodies  >8 publications with PMEL and Testis
 Liver Antibodies  >8 publications with PMEL and Liver
 Muscle Antibodies  >7 publications with PMEL and Muscle
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