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anti-MAP1LC3a antibody :: Rabbit anti-Human Microtubule Associated Protein 1 Light Chain 3 Alpha Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS2034049 (SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
0.01 mg  /  $115 +1 FREE 8GB USB
0.02 mg  /  $135 +1 FREE 8GB USB
0.05 mg  /  $190 +1 FREE 8GB USB
0.1 mg  /  $245 +1 FREE 8GB USB
0.2 mg  /  $370 +1 FREE 8GB USB
1 mg  /  $880 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Microtubule Associated Protein 1 Light Chain 3 Alpha (MAP1LC3a), Polyclonal Antibody

★Popular Item★
 Also Known As   

Polyclonal Antibody to Microtubule Associated Protein 1 Light Chain 3 Alpha (MAP1LC3a)

 Product Synonym Names    MAP1ALC3; MAP1BLC3; LC3; LC3A; ATG8E; Autophagy-related ubiquitin-like modifier LC3 A; MAP1 light chain 3-like protein 1
 Product Gene Name   

anti-MAP1LC3a antibody

[Similar Products]
 Matching Pairs    Antibody: Microtubule Associated Protein 1 Light Chain 3 Alpha (MBS2034049)
Antigen: Microtubule Associated Protein 1 Light Chain 3 Alpha (MBS2034376)
 Matching Pairs    Unconjugated Antibody: Microtubule Associated Protein 1 Light Chain 3 Alpha (MBS2034049)
APC-CY7 Conjugated Antibody: Microtubule Associated Protein 1 Light Chain 3 Alpha (MAP1LC3a) (MBS2083183)
 Matching Pairs    Unconjugated Antibody: Microtubule Associated Protein 1 Light Chain 3 Alpha (MBS2034049)
PE Conjugated Antibody: Microtubule Associated Protein 1 Light Chain 3 Alpha (MAP1LC3a) (MBS2083184)
 Matching Pairs    Unconjugated Antibody: Microtubule Associated Protein 1 Light Chain 3 Alpha (MBS2034049)
APC-CY7 Conjugated Antibody: Microtubule Associated Protein 1 Light Chain 3 Alpha (MAP1LC3a) (MBS2083185)
 Matching Pairs    Unconjugated Antibody: Microtubule Associated Protein 1 Light Chain 3 Alpha (MBS2034049)
Cy3 Conjugated Antibody: Microtubule Associated Protein 1 Light Chain 3 Alpha (MAP1LC3a) (MBS2083186)
 Matching Pairs    Unconjugated Antibody: Microtubule Associated Protein 1 Light Chain 3 Alpha (MBS2034049)
FITC Conjugated Antibody: Microtubule Associated Protein 1 Light Chain 3 Alpha (MAP1LC3a) (MBS2083187)
 Matching Pairs    Unconjugated Antibody: Microtubule Associated Protein 1 Light Chain 3 Alpha (MBS2034049)
HRP Conjugated Antibody: Microtubule Associated Protein 1 Light Chain 3 Alpha (MAP1LC3a) (MBS2083188)
 Matching Pairs    Unconjugated Antibody: Microtubule Associated Protein 1 Light Chain 3 Alpha (MBS2034049)
APC-CY7 Conjugated Secondary Antibody: Immunoglobulin G (MBS2090675)
 Matching Pairs    Unconjugated Antibody: Microtubule Associated Protein 1 Light Chain 3 Alpha (MBS2034049)
Unconjugated Secondary Antibody: Immunoglobulin G (MBS2090678)
 Matching Pairs    Unconjugated Antibody: Microtubule Associated Protein 1 Light Chain 3 Alpha (MBS2034049)
Biotin Conjugated Antibody: Microtubule Associated Protein 1 Light Chain 3 Alpha (MAP1LC3a) (MBS2096875)
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    AF276658 mRNA
 3D Structure    ModBase 3D Structure for Q9H492
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 Clonality    Polyclonal
 Isotype    IgG
 Host    Rabbit
 Species Reactivity    Human
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 Specificity    The antibody is a rabbit polyclonal antibody raised against MAP1LC3a. It has been selected for its ability to recognize MAP1LC3a in immunohistochemical staining and western blotting.
 Purity/Purification    Affinity Chromatography
 Form/Format    Supplied as solution form in PBS, pH7.4, Containing 0.02% NaN3, 50% glycerol.
 Concentration    200ug/ml (lot specific)
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 Fragment    MAP1LC3a (Met1~Gly120)
 Conjugate    No Conjugate
 Immunoglobulin Type    IgG
 Quality Control    Content: The quality control contains recombinant MAP1LC3a (Met1~Gly120) disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control. Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
 Conjugated Antibody    The APC conjugated antibody version of this item is also available as catalog #MBS2083185
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 Preparation and Storage    Store at 4 degree C for frequent use. Stored at -20 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
 ISO Certification    Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
 Supply Chain Verification    Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available. Please inquire.
 Other Notes    Small volumes of anti-MAP1LC3a antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Applications Tested/Suitable for anti-MAP1LC3a antibody   

Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)

 Application Notes for anti-MAP1LC3a antibody    Western blotting: 1:50-400
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-1:5000
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 Western Blot (WB) of anti-MAP1LC3a antibody    (Figure. Western Blot; Sample: Lane1: Rat Brain Tissue; Lane2: Mouse Brain Tissue.)
anti-MAP1LC3a antibody Western Blot (WB) (WB) image
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 Western Blot (WB) of anti-MAP1LC3a antibody    (Figure. Western Blot; Sample: Recombinant MAP1LC3a, Human.)
anti-MAP1LC3a antibody Western Blot (WB) (WB) image
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NCBI/Uniprot data below describe general gene information for MAP1LC3a. It may not necessarily be applicable to this product.
 NCBI GI #    14210522
 NCBI GeneID    84557
 NCBI Accession #    NP_115903.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_032514.3 [Other Products]
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 UniProt Primary Accession #    Q9H492 [Other Products]
 UniProt Secondary Accession #    Q9BXW5; E1P5P4; E1P5P5 [Other Products]
 UniProt Related Accession #    Q9H492 [Other Products]
 Molecular Weight    14,493 Da
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 NCBI Official Full Name    microtubule-associated proteins 1A/1B light chain 3A isoform a
 NCBI Official Synonym Full Names    microtubule associated protein 1 light chain 3 alpha
 NCBI Official Symbol    MAP1LC3A [Similar Products]
 NCBI Official Synonym Symbols   
LC3; LC3A; ATG8E; MAP1ALC3; MAP1BLC3
[Similar Products]
 NCBI Protein Information    microtubule-associated proteins 1A/1B light chain 3A
 UniProt Protein Name    Microtubule-associated proteins 1A/1B light chain 3A
 UniProt Synonym Protein Names   
Autophagy-related protein LC3 A; Autophagy-related ubiquitin-like modifier LC3 A; MAP1 light chain 3-like protein 1; MAP1A/MAP1B light chain 3 A; MAP1A/MAP1B LC3 A; Microtubule-associated protein 1 light chain 3 alpha
 Protein Family    Microtubule-associated protein
 UniProt Gene Name    MAP1LC3A [Similar Products]
 UniProt Synonym Gene Names    MAP1A/MAP1B LC3 A [Similar Products]
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 NCBI Summary for MAP1LC3a    MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. The protein encoded by this gene is one of the light chain subunits and can associate with either MAP1A or MAP1B. Two transcript variants encoding different isoforms have been found for this gene. The expression of variant 1 is suppressed in many tumor cell lines, suggesting that may be involved in carcinogenesis. [provided by RefSeq, Feb 2012]
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 UniProt Comments for MAP1LC3a    LC3A: is a ubiquitin-like protein that is a constituent of the ATG8-conjugation system, one of two evolutionarily conserved phosphatidylethanolamine conjugation systems necessary for the formation of the autophagosome. The human ATG8 system includes seven ubiquitin-like light chain proteins (LCPs) that are homologs of yeast LC3: MAP1LC3A, -B, -C, GABARAP, GABARAPL1, -2, and -3. Pro-LCPs are cleaved by ATG4B to expose a C-terminal glycine residue, the cytosolic LCP-I form. The exposed C-terminus is conjugated to the head group amine of phosphatidylethanolamine (PE) through an amide bond by a sequence of ubiquitination-like reactions that involves an E1 (ATG7), an E2 (ATG3), and an E3 (a complex including ATG5, ATG12, and ATG16L). The PE-congugated form (LCP-II) is tightly associated with the autophagosomal membrane. The LCP-II forms can also be delipidated by the ATG4 proteases: most of the LCPs are delipidated and liberated from the membrane before autophagosomes fuse with lysosomes. Two isoforms of the human protein are produced by alternative splicing.

Protein type: Autophagy; Microtubule-binding; Ubiquitin-like modifier; Vesicle

Chromosomal Location of Human Ortholog: 20q11.22

Cellular Component: autophagic vacuole; cytosol; late endosome; microtubule

Molecular Function: phosphatidylethanolamine binding; phospholipid binding; protein binding; ubiquitin protein ligase binding

Biological Process: autophagosome assembly; cellular response to amino acid starvation; cellular response to nitrogen starvation; cellular response to starvation; macroautophagy; mitochondrion degradation; response to iron(II) ion; response to lead ion; response to morphine
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 Research Articles on MAP1LC3a    1. we found that ATG14 interacted with Ulk1 and LC3, and knock down of Ulk1 prevented the lipidation of LC3 and autophagy in HeLa-ATG14 cells. We also identified a phosphatidylethanolamine (PE) binding region in ATG14, and the addition of Ulk1 to Hela-ATG14 cells decreased the ATG14-PE interaction.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-MAP1LC3a antibodyPathways associated with anti-MAP1LC3a antibody
 Reference Product  PubMed Publications
 ATG7 antibody  >5 publications with MAP1LC3a and ATG7
 SQSTM1 antibody  >4 publications with MAP1LC3a and SQSTM1
 ATG4B antibody  >2 publications with MAP1LC3a and ATG4B
 ATG3 antibody  >2 publications with MAP1LC3a and ATG3
 Products by Pathway  Pathway Diagram
 Senescence And Autophagy Pathway antibodies  Senescence And Autophagy Pathway Diagram
Diseases associated with anti-MAP1LC3a antibodyOrgans/Tissues associated with anti-MAP1LC3a antibody
 Disease Name  Pubmed Publications
 Brain Diseases Antibodies  >4 publications with MAP1LC3a and Brain Diseases
 Nervous System Diseases Antibodies  >4 publications with MAP1LC3a and Nervous System Diseases
 Neoplasm Metastasis Antibodies  >2 publications with MAP1LC3a and Neoplasm Metastasis
 Liver Neoplasms Antibodies  >2 publications with MAP1LC3a and Liver Neoplasms
 Urinary Bladder Neoplasms Antibodies  >1 publications with MAP1LC3a and Urinary Bladder Neoplasms
 Carcinoma, Hepatocellular Antibodies  >1 publications with MAP1LC3a and Carcinoma, Hepatocellular
 Liver Diseases Antibodies  >1 publications with MAP1LC3a and Liver Diseases
 Inflammation Antibodies  >1 publications with MAP1LC3a and Inflammation
 Hyperplasia Antibodies  >1 publications with MAP1LC3a and Hyperplasia
 Disease Models, Animal Antibodies  >1 publications with MAP1LC3a and Disease Models, Animal
 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >5 publications with MAP1LC3a and Lung
 Brain Antibodies  >5 publications with MAP1LC3a and Brain
 Liver Antibodies  >2 publications with MAP1LC3a and Liver
 Kidney Antibodies  >2 publications with MAP1LC3a and Kidney
 Uterus Antibodies  >2 publications with MAP1LC3a and Uterus
 Intestine Antibodies  >2 publications with MAP1LC3a and Intestine
 Muscle Antibodies  >2 publications with MAP1LC3a and Muscle
 Testis Antibodies  >1 publications with MAP1LC3a and Testis
 Prostate Antibodies  >1 publications with MAP1LC3a and Prostate
 Embryonic Tissue Antibodies  >1 publications with MAP1LC3a and Embryonic Tissue
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