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anti-PRKDC antibody :: Rabbit Myosin Light Chain 2 Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS857334
Unit / Price
0.1 mg  /  $215 +1 FREE 8GB USB
0.05 mg  /  $225 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Myosin Light Chain 2 (PRKDC), Polyclonal Antibody

 Also Known As   

Myosin Light Chain 2 (Phospho-Ser18) Antibody

 Product Gene Name   

anti-PRKDC antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    600899
 3D Structure    ModBase 3D Structure for P78527
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 Clonality    Polyclonal
 Isotype    IgG
 Host    Rabbit
 Species Reactivity    Human, Mouse, Rat
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 Specificity    Myosin Light Chain 2 (Phospho-Ser18) Antibody detects endogenous levels of Myosin Light Chain 2 only when phosphorylated at serine 18.
 Purity/Purification    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation
 Form/Format    Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
 Concentration    50ug/50ul (lot specific)
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 Immunogen    The antiserum was produced against synthesized phosphopeptide derived from human Myosin Light Chain 2 around the phosphorylation site of serine 18 (A-T-SP-N-V).
 Pathway    Cytoskeletal/Adhesion
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 Preparation and Storage    Store at -20 degree C for 1 year
 Other Notes    Small volumes of anti-PRKDC antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Product Categories/Family for anti-PRKDC antibody    Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells; Autophagy antibody
 Applications Tested/Suitable for anti-PRKDC antibody   

Immunohistochemistry (IHC)

 Application Notes for anti-PRKDC antibody    WB: 1:500~1:1000, IHC: 1:50~1:100
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 Testing Data of anti-PRKDC antibody    P-Peptide - +
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Myosin Light Chain 2
(Phospho-Ser18) Antibody (E011114).
anti-PRKDC antibody Testing Data image
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 Testing Data #2 of anti-PRKDC antibody    Western blot analysis of extracts from mouse heart tissue
using Myosin Light Chain 2 (Phospho-Ser18) Antibody
(E011114).
anti-PRKDC antibody Testing Data #2 image
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NCBI/Uniprot data below describe general gene information for PRKDC. It may not necessarily be applicable to this product.
 NCBI GI #    126032350
 NCBI GeneID    5591
 NCBI Accession #    NP_001075109.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001081640.1 [Other Products]
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 UniProt Primary Accession #    P78527 [Other Products]
 UniProt Secondary Accession #    P78528; Q13327; Q13337; Q14175; Q59H99; Q7Z611; Q96SE6; Q9UME3 [Other Products]
 UniProt Related Accession #    P78527 [Other Products]
 Molecular Weight    465,501 Da [Similar Products]
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 NCBI Official Full Name    DNA-dependent protein kinase catalytic subunit isoform 2
 NCBI Official Synonym Full Names    protein kinase, DNA-activated, catalytic polypeptide
 NCBI Official Symbol    PRKDC [Similar Products]
 NCBI Official Synonym Symbols   
HYRC; p350; DNAPK; DNPK1; HYRC1; IMD26; XRCC7; DNA-PKcs
[Similar Products]
 NCBI Protein Information    DNA-dependent protein kinase catalytic subunit; p460; DNA-PK catalytic subunit; hyper-radiosensitivity of murine scid mutation, complementing 1
 UniProt Protein Name    DNA-dependent protein kinase catalytic subunit
 UniProt Synonym Protein Names   
DNPK1; p460
 Protein Family    Myosin
 UniProt Gene Name    PRKDC [Similar Products]
 UniProt Synonym Gene Names    HYRC; HYRC1; DNA-PK catalytic subunit; DNA-PKcs [Similar Products]
 UniProt Entry Name    PRKDC_HUMAN
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 NCBI Summary for PRKDC    This gene encodes the catalytic subunit of the DNA-dependent protein kinase (DNA-PK). It functions with the Ku70/Ku80 heterodimer protein in DNA double strand break repair and recombination. The protein encoded is a member of the PI3/PI4-kinase family.[provided by RefSeq, Jul 2010]
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 UniProt Comments for PRKDC    DNAPK: an atypical protein kinase of the PIKK family. Involved in DNA nonhomologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. and modulation of transcription. Must be bound to DNA to express its catalytic properties. Promotes processing of hairpin DNA structures in V(D)J recombination by activation of the hairpin endonuclease artemis (DCLRE1C). The assembly of the DNA-PK complex at DNA ends is also required for the NHEJ ligation step. Required to protect and align broken ends of DNA. May also act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage. Found at the ends of chromosomes, suggesting a further role in the maintenance of telomeric stability and the prevention of chromosomal end fusion. Also involved in modulation of transcription. Defects cause severe combined immune deficiency (SCID) which is characterized by a lack of mature functional lymphocytes and a high susceptibility to lethal opportunistic infections. Required for repair of radiation-induced dsDNA breaks. Loss of function in mice or horses leads to the SCID (severe combined immune deficiency) phenotype due to failure of immunoglobulin rearrangement. Target of mutation in mismatch repair-deficient colorectal cancer. Inhibitor: KU-7059. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA repair, damage; EC 2.7.11.1; Nucleolus; Protein kinase, atypical; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); ATYPICAL group; PIKK family; DNAPK subfamily

Chromosomal Location of Human Ortholog: 8q11

Cellular Component: nucleoplasm; transcription factor complex; membrane; nucleolus; DNA-dependent protein kinase complex; cytosol

Molecular Function: protein serine/threonine kinase activity; protein binding; enzyme binding; DNA binding; DNA-dependent protein kinase activity; transcription factor binding; ATP binding; protein kinase activity

Biological Process: positive regulation of apoptosis; heart development; germ cell programmed cell death; rhythmic process; T cell differentiation in the thymus; double-strand break repair via nonhomologous end joining; negative regulation of protein amino acid phosphorylation; double-strand break repair; positive regulation of interferon type I production; response to gamma radiation; telomere maintenance; pro-B cell differentiation; somitogenesis; immunoglobulin V(D)J recombination; protein destabilization; B cell lineage commitment; protein modification process; regulation of circadian rhythm; DNA repair; double-strand break repair via homologous recombination; T cell lineage commitment; peptidyl-serine phosphorylation; DNA damage response, signal transduction resulting in induction of apoptosis; cellular response to insulin stimulus; innate immune response; T cell receptor V(D)J recombination; positive regulation of transcription from RNA polymerase II promoter; brain development

Disease: Immunodeficiency 26 With Or Without Neurologic Abnormalities
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Product References and Citations for anti-PRKDC antibody

   Janiak A, et al. (2006) Mol Biol Cell. Apr; 17(4): 1606-1619.
Croft DR, et al. (2006) Mol Cell Biol. 2 Jun; 26(12): 4612-4627
Li Z, et al. (2006) Mol Cell Biol. Jun; 26(11): 4240-4256
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 Research Articles on PRKDC    1. Indicate that phosphorylation of Ser-440 and -467 in WRN by DNA-PK is important for relocalization of WRN to nucleoli, and that it is required for efficient DSB repair.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with anti-PRKDC antibodyDiseases associated with anti-PRKDC antibody
 Products by Pathway  Pathway Diagram
 BARD1 Signaling Events Pathway antibodies  BARD1 Signaling Events Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Class I PI3K Signaling Events Mediated By Akt Pathway antibodies  Class I PI3K Signaling Events Mediated By Akt Pathway Diagram
 Coregulation Of Androgen Receptor Activity Pathway antibodies  Coregulation Of Androgen Receptor Activity Pathway Diagram
 Cytosolic Sensors Of Pathogen-associated DNA Pathway antibodies  Cytosolic Sensors Of Pathogen-associated DNA Pathway Diagram
 DNA Repair Pathway antibodies  DNA Repair Pathway Diagram
 DNA-PK Complex Pathway antibodies  DNA-PK Complex Pathway Diagram
 DNA-PK Complex Pathway antibodies  DNA-PK Complex Pathway Diagram
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >50 publications with PRKDC and Nervous System Diseases
 Disease Models, Animal Antibodies  >49 publications with PRKDC and Disease Models, Animal
 Chromosome Aberrations Antibodies  >49 publications with PRKDC and Chromosome Aberrations
 Breast Neoplasms Antibodies  >33 publications with PRKDC and Breast Neoplasms
 Lymphoma Antibodies  >31 publications with PRKDC and Lymphoma
 Neoplasms, Experimental Antibodies  >25 publications with PRKDC and Neoplasms, Experimental
 Adenocarcinoma Antibodies  >25 publications with PRKDC and Adenocarcinoma
 Lung Neoplasms Antibodies  >20 publications with PRKDC and Lung Neoplasms
 Lung Diseases Antibodies  >19 publications with PRKDC and Lung Diseases
 Prostatic Neoplasms Antibodies  >15 publications with PRKDC and Prostatic Neoplasms
Organs/Tissues associated with anti-PRKDC antibody
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >121 publications with PRKDC and Blood
 Brain Antibodies  >40 publications with PRKDC and Brain
 Bone Antibodies  >34 publications with PRKDC and Bone
 Lung Antibodies  >33 publications with PRKDC and Lung
 Liver Antibodies  >32 publications with PRKDC and Liver
 Bone Marrow Antibodies  >29 publications with PRKDC and Bone Marrow
 Spleen Antibodies  >24 publications with PRKDC and Spleen
 Skin Antibodies  >17 publications with PRKDC and Skin
 Prostate Antibodies  >15 publications with PRKDC and Prostate
 Kidney Antibodies  >14 publications with PRKDC and Kidney
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