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anti-MYLK antibody :: Rabbit Myosin Light Chain Kinase, Smooth Muscle Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS618405 anti-MYLK antibody
Unit / Price
10 Blots  /  $620 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Myosin Light Chain Kinase, Smooth Muscle (MYLK), Polyclonal Antibody

 Also Known As   

Myosin Light Chain Kinase, Smooth Muscle, phosphorylated (Ser1760) (MLCK)

 Product Synonym Names    Anti -Myosin Light Chain Kinase, Smooth Muscle, phosphorylated (Ser1760) (MLCK)
 Product Gene Name   

anti-MYLK antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Chromosome Location    Chromosome: 3; NC_000003.11 (123331143..123603149, complement). Location: 3q21
 OMIM    600922
 3D Structure    ModBase 3D Structure for Q15746
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 Clonality    Polyclonal
 Isotype    IgG
 Host    Rabbit
 Species Reactivity    Bovine, Mouse, Rabbit
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 Specificity    Recognizes MLCK when phosphorylated at Ser1760. Species Crossreactivity
 Purity/Purification    Affinity Purified
Purified by immunoaffinity chromatography.
 Form/Format    Supplied as a liquid in Dulbecco's PBS (without Mg2+ and Ca2+), pH 7.3, 1mg/ml BSA (IgG, protease free), 0.05% sodium azide, 50% glycerol.
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 Immunogen    Synthetic phosphopeptide derived from the region of human myosin light chain kinase (MLCK) that contains serine 1760.
 Positive Control    KCl-treated rabbit muscle extracts; COS cells over-expressing rabbit smooth muscle short MLCK, phosphorylated in vitro by PKA.
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 Preparation and Storage    May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
 Other Notes    Small volumes of anti-MYLK antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-MYLK antibody

   Smooth Muscle Myosin Light Chain Kinase (MLCK) is a multifunctional regulatory protein of smooth muscle contraction (SMC) and a key element in ligand-mediated endothelial cell gap formation and vascular permeability, motility and morphology. Smooth muscle MLCK exists in at least two isoforms, short (~150kD) and long (210kD) which are identical except for an extended amino terminus with two additional putative actin-binding motifs in the long isoform.
 Product Categories/Family for anti-MYLK antibody    Antibodies; Abs to Myosin
 Applications Tested/Suitable for anti-MYLK antibody   

Western Blot (WB)

 Application Notes for anti-MYLK antibody    Suitable for use in Western Blot.
Dilution: Western Blot: 1:1000
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NCBI/Uniprot data below describe general gene information for MYLK. It may not necessarily be applicable to this product.
 NCBI GI #    116008192
 NCBI GeneID    4638
 NCBI Accession #    NP_444253.3 [Other Products]
 NCBI GenBank Nucleotide #    NM_053025.3 [Other Products]
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 UniProt Primary Accession #    Q15746 [Other Products]
 UniProt Secondary Accession #    O95796; O95797; O95798; O95799; Q14844; Q16794; Q5MY99; Q5MYA0; Q7Z4J0; Q9C0L5; Q9UBG5 [Other Products]
 UniProt Related Accession #    Q05B97; Q05B98; Q05D81; Q06S79; Q15746; Q17S15; Q3B765; Q3ZCP9; Q68DK3; Q6LAL4 [Other Products]
 Molecular Weight    210,715 Da [Similar Products]
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 NCBI Official Full Name    myosin light chain kinase, smooth muscle isoform 1
 NCBI Official Synonym Full Names    myosin light chain kinase
 NCBI Official Symbol    MYLK [Similar Products]
 NCBI Official Synonym Symbols   
KRP; AAT7; MLCK; MLCK1; MYLK1; smMLCK; MLCK108; MLCK210; MSTP083; FLJ12216; DKFZp686I10125
[Similar Products]
 NCBI Protein Information    myosin light chain kinase, smooth muscle; telokin; OTTHUMP00000180642; OTTHUMP00000180643; OTTHUMP00000215974; OTTHUMP00000225212; kinase-related protein; myosin, light polypeptide kinase; smooth muscle myosin light chain kinase
 UniProt Protein Name    Myosin light chain kinase, smooth muscle
 UniProt Synonym Protein Names   
Kinase-related protein; KRP; Telokin
 Protein Family    Myosin light chain kinase
 UniProt Gene Name    MYLK [Similar Products]
 UniProt Synonym Gene Names    MLCK [Similar Products]
 UniProt Entry Name    MYLK_HUMAN
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 NCBI Summary for MYLK    This gene, a muscle member of the immunoglobulin gene superfamily, encodes myosin light chain kinase which is a calcium/calmodulin dependent enzyme. This kinase phosphorylates myosin regulatory light chains to facilitate myosin interaction with actin filaments to produce contractile activity. This gene encodes both smooth muscle and nonmuscle isoforms. In addition, using a separate promoter in an intron in the 3' region, it encodes telokin, a small protein identical in sequence to the C-terminus of myosin light chain kinase, that is independently expressed in smooth muscle and functions to stabilize unphosphorylated myosin filaments. A pseudogene is located on the p arm of chromosome 3. Four transcript variants that produce four isoforms of the calcium/calmodulin dependent enzyme have been identified as well as two transcripts that produce two isoforms of telokin. Additional variants have been identified but lack full length transcripts. [provided by RefSeq]
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 UniProt Comments for MYLK    smMLCK: Calcium/calmodulin-dependent myosin light chain kinase implicated in smooth muscle contraction via phosphorylation of myosin light chains (MLC). Also regulates actin-myosin interaction through a non-kinase activity. Phosphorylates PTK2B/PYK2 and myosin light-chains. Involved in the inflammatory response (e.g. apoptosis, vascular permeability, leukocyte diapedesis), cell motility and morphology, airway hyperreactivity and other activities relevant to asthma. Required for tonic airway smooth muscle contraction that is necessary for physiological and asthmatic airway resistance. Necessary for gastrointestinal motility. Implicated in the regulation of endothelial as well as vascular permeability, probably via the regulation of cytoskeletal rearrangements. In the nervous system it has been shown to control the growth initiation of astrocytic processes in culture and to participate in transmitter release at synapses formed between cultured sympathetic ganglion cells. Critical participant in signaling sequences that result in fibroblast apoptosis. Plays a role in the regulation of epithelial cell survival. Required for epithelial wound healing, especially during actomyosin ring contraction during purse-string wound closure. Mediates RhoA- dependent membrane blebbing. Triggers TRPC5 channel activity in a calcium-dependent signaling, by inducing its subcellular localization at the plasma membrane. Promotes cell migration (including tumor cells) and tumor metastasis. PTK2B/PYK2 activation by phosphorylation mediates ITGB2 activation and is thus essential to trigger neutrophil transmigration during acute lung injury (ALI). May regulate optic nerve head astrocyte migration. Probably involved in mitotic cytoskeletal regulation. Regulates tight junction probably by modulating ZO-1 exchange in the perijunctional actomyosin ring. Mediates burn-induced microvascular barrier injury; triggers endothelial contraction in the development of microvascular hyperpermeability by phosphorylating MLC. Essential for intestinal barrier dysfunction. Mediates Giardia spp.-mediated reduced epithelial barrier function during giardiasis intestinal infection via reorganization of cytoskeletal F-actin and tight junctional ZO-1. Necessary for hypotonicity-induced Ca(2+) entry and subsequent activation of volume-sensitive organic osmolyte/anion channels (VSOAC) in cervical cancer cells. Responsible for high proliferative ability of breast cancer cells through anti-apoptosis. Defects in MYLK are the cause of familial aortic aneurysm thoracic type 7 (AAT7). AAT7 is a disease characterized by permanent dilation of the thoracic aorta usually due to degenerative changes in the aortic wall. It is primarily associated with a characteristic histologic appearance known as 'medial necrosis' or 'Erdheim cystic medial necrosis' in which there is degeneration and fragmentation of elastic fibers, loss of smooth muscle cells, and an accumulation of basophilic ground substance. Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. 8 isoforms of the human protein are produced by alternative splicing.

Protein type: Protein kinase, Ser/Thr (non-receptor); Protein kinase, CAMK; Kinase, protein; EC 2.7.11.18; CAMK group; MLCK family

Chromosomal Location of Human Ortholog: 3q21

Cellular Component: lamellipodium; cytoplasm; stress fiber; intercellular junction; cytosol; cleavage furrow

Molecular Function: calmodulin binding; protein binding; calmodulin-dependent protein kinase activity; metal ion binding; actin binding; ATP binding; myosin light chain kinase activity

Biological Process: tonic smooth muscle contraction; smooth muscle contraction; muscle contraction; actin filament organization; bleb formation; positive regulation of calcium ion transport; protein amino acid phosphorylation; positive regulation of cell migration

Disease: Aortic Aneurysm, Familial Thoracic 7
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 Research Articles on MYLK    1. Data identify a novel interaction between cardiac-specific Ig-like domain C0 and the regulatory light chain of myosin, thus placing the N terminus of the protein in proximity to the motor domain of myosin.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-MYLK antibodyPathways associated with anti-MYLK antibody
 Reference Product  PubMed Publications
 PRKG2 antibody  >1 publications with MYLK and PRKG2
 CTTN antibody  >1 publications with MYLK and CTTN
 Products by Pathway  Pathway Diagram
 Aurora B Signaling Pathway antibodies  Aurora B Signaling Pathway Diagram
 Calcium Signaling Pathway antibodies  Calcium Signaling Pathway Diagram
 Calcium Signaling Pathway antibodies  Calcium Signaling Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 Gastric Acid Secretion Pathway antibodies  Gastric Acid Secretion Pathway Diagram
 Gastric Acid Secretion Pathway antibodies  Gastric Acid Secretion Pathway Diagram
 MicroRNAs In Cardiomyocyte Hypertrophy Pathway antibodies  MicroRNAs In Cardiomyocyte Hypertrophy Pathway Diagram
 Muscle Contraction Pathway antibodies  Muscle Contraction Pathway Diagram
Diseases associated with anti-MYLK antibodyOrgans/Tissues associated with anti-MYLK antibody
 Disease Name  Pubmed Publications
 Lung Diseases Antibodies  >10 publications with MYLK and Lung Diseases
 Lung Injury Antibodies  >7 publications with MYLK and Lung Injury
 Acute Lung Injury Antibodies  >7 publications with MYLK and Acute Lung Injury
 Inflammation Antibodies  >6 publications with MYLK and Inflammation
 Respiratory Hypersensitivity Antibodies  >5 publications with MYLK and Respiratory Hypersensitivity
 Neoplasms Antibodies  >4 publications with MYLK and Neoplasms
 Vascular Diseases Antibodies  >4 publications with MYLK and Vascular Diseases
 Respiratory Distress Syndrome, Adult Antibodies  >3 publications with MYLK and Respiratory Distress Syndrome, Adult
 Arteriosclerosis Antibodies  >2 publications with MYLK and Arteriosclerosis
 Pneumonia Antibodies  >2 publications with MYLK and Pneumonia
 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >19 publications with MYLK and Muscle
 Lung Antibodies  >10 publications with MYLK and Lung
 Vascular Antibodies  >10 publications with MYLK and Vascular
 Blood Antibodies  >4 publications with MYLK and Blood
 Eye Antibodies  >3 publications with MYLK and Eye
 Intestine Antibodies  >2 publications with MYLK and Intestine
 Nerve Antibodies  >2 publications with MYLK and Nerve
 Spleen Antibodies  >1 publications with MYLK and Spleen
 Liver Antibodies  >1 publications with MYLK and Liver
 Stomach Antibodies  >1 publications with MYLK and Stomach
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