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anti-SLC12A3 antibody :: Rabbit NCC Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS805451
Unit / Price
0.1 mg  /  $450 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

NCC (SLC12A3), Polyclonal Antibody

 Also Known As   

NCC Antibody: ATTO 594; Rabbit Anti-Rat NCC Antibody Polyclonal

 Product Synonym Names    SLC12A3 Antibody, SCYL1 Antibody,CKb10 Antibody, MCP-4 Antibody, MGC17134 Antibody, NCC-1 Antibody, NCC1 Antibody, SCYA13 Antibody, CK-beta-10 Antibody, monocyte chemoattractant protein 4 Antibody, monocyte chemotactic protein 4 Antibody, new CC chemokine 1 Antibody, small inducible cytokine A13 Antibody, small inducible cytokine subfamily A (Cys-Cys) member 13 Antibody, chemokine (C-C) Antibody
 Product Gene Name   

anti-SLC12A3 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 3D Structure    ModBase 3D Structure for P55018
 Clonality    Polyclonal
 Host    Rabbit
 Species Reactivity    Human, Mouse, Rat, Dog
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 Specificity    Detects ~160kDa
 Purity/Purification    Affinity Purified
 Concentration    1mg/mL (lot specific)
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 Conjugate    ATTO 594
 Immunogen    Produced against a synthetic peptide mapping to a segment of rat NCC (amino acids 74-95), N-terminal.
 Certificate of Analysis    1ug/ml of MBS805451 was sufficient for detection of NCC3 in 10 ug of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-Rabbit IgG: HRP as the secondary antibody.
 Research Areas    Cancer, Neuroscience, Pumps/Transporters, Ion Pumps/Transporters.
 Cellular Localization    Membrane
 Sequence References    Gene ID:54300,6559
 Storage Buffer    PBS, 50% glycerol, 0.09% sodium azide
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 Preparation and Storage    Store at -20C; 1 year+ Avoid freeze/thaw cycles.
Shipping: Blue Ice or 4C
 Other Notes    Small volumes of anti-SLC12A3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Product Description specifically for anti-SLC12A3 antibody

   Function: NCC, a thiazide-sensitive NaCl co-transporter, is found on the apical membrane of the distal convoluted tubule, where it is the principal mediator of Na+ and Cl- reabsorption in this segment of the nephron. It is activated by phosphorylation, and has been implicated in renal NaCl and K+ homeostasis (1). Regulation of NCC expression and phosphorylation by dietary NACl restriction appears to involve SGK1(1). In experiments with angiotensin II-infused mice, increased sensitivity to Ang II may involved over-activity of NCC (2). Therefore, NCC is the target of thiazide diuretics used in the treatment of hypertension (1). Molecular experiments have also shown that NCC has been detected in the lens cortex, core and fiber cells of a rat (3).
 Product Categories/Family for anti-SLC12A3 antibody    Ion Channels, Transporters
 Applications Tested/Suitable for anti-SLC12A3 antibody   

Western Blot (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), IEM

 Application Notes for anti-SLC12A3 antibody    WB: 1:1000
Optimal dilutions for assays should be determined by the end user.
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 Testing Data of anti-SLC12A3 antibody    Western blot analysis of NCC in rat tissues using a 1:1000 dilution.
anti-SLC12A3 antibody Testing Data image
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 Testing Data #2 of anti-SLC12A3 antibody    IF analysis of NCC in rat kidney tissues, showing using a 1:200 dilution. DAPI merge.
anti-SLC12A3 antibody Testing Data #2 image
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NCBI/Uniprot data below describe general gene information for SLC12A3. It may not necessarily be applicable to this product.
 NCBI GI #    186910315
 NCBI GeneID    6559
 NCBI Accession #    NP_000330 [Other Products]
 NCBI Related Accession #    NP_062218 [Other Products]
 NCBI GenBank Nucleotide #    NM_000339.2 [Other Products]
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 UniProt Primary Accession #    P55018 [Other Products]
 UniProt Secondary Accession #    P55017 [Other Products]
 UniProt Related Accession #    P55017 [Other Products]
 Molecular Weight    ~160kDa [Similar Products]
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 NCBI Official Full Name    solute carrier family 12 member 3 isoform 1
 NCBI Official Synonym Full Names    solute carrier family 12 (sodium/chloride transporter), member 3
 NCBI Official Symbol    SLC12A3 [Similar Products]
 NCBI Official Synonym Symbols   
NCC; TSC; NCCT
[Similar Products]
 NCBI Protein Information    solute carrier family 12 member 3; Na-Cl symporter; Na-Cl cotransporter; thiazide-sensitive Na-Cl cotransporter; thiazide-sensitive sodium-chloride cotransporter; NaCl electroneutral thiazide-sensitive cotransporter
 UniProt Protein Name    Solute carrier family 12 member 3
 UniProt Synonym Protein Names   
Na-Cl symporter; Thiazide-sensitive sodium-chloride cotransporter
 Protein Family    Solute carrier family
 UniProt Gene Name    Slc12a3 [Similar Products]
 UniProt Synonym Gene Names    Tsc [Similar Products]
 UniProt Entry Name    S12A3_RAT
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 NCBI Summary for SLC12A3    This gene encodes a renal thiazide-sensitive sodium-chloride cotransporter that is important for electrolyte homeostasis. This cotransporter mediates sodium and chloride reabsorption in the distal convoluted tubule. Mutations in this gene cause Gitelman syndrome, a disease similar to Bartter's syndrome, that is characterized by hypokalemic alkalosis combined with hypomagnesemia, low urinary calcium, and increased renin activity associated with normal blood pressure. This cotransporter is the target for thiazide diuretics that are used for treating high blood pressure. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
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 UniProt Comments for SLC12A3    NCCT: Key mediator of sodium and chloride reabsorption in this nephron segment, accounting for a significant fraction of renal sodium reabsorption. Interacts with KLHL3. Predominant in kidney. Activated by WNK3. Belongs to the SLC12A transporter family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Transporter, SLC family; Transporter; Membrane protein, integral

Cellular Component: apical plasma membrane; plasma membrane; integral to membrane; cytosol; vesicle

Molecular Function: sodium ion transmembrane transporter activity; transporter activity; sodium:chloride symporter activity; cation:chloride symporter activity

Biological Process: transport; sodium ion transport; chloride transport
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Product References and Citations for anti-SLC12A3 antibody

   1. Vallon V., Schroth J, Lang F, Kuhl D and Uchida S. (2009) Am J Physiol Renal Physiol. 297(3): F704-712. 2. Tiwari S., et al. (2009) Am J Nephrol. 30(6): 554-562. 3. Chee K.N., Vorontsova I., Lim J.C., Kistler J. and Donaldson P.J. (2010) Mol Vis. 16:800-812.
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 Research Articles on SLC12A3    1. Association with SNP rs999662 indicates a potential role for the region containing the solute carrier family 12 member 3 (SLC12A3) gene in transcranial Doppler vasospasm following sub-arachnoid hemorrhage.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-SLC12A3 antibodyPathways associated with anti-SLC12A3 antibody
 Reference Product  PubMed Publications
 Clcnkb antibody  >32 publications with SLC12A3 and Clcnkb
 Wnk4 antibody  >28 publications with SLC12A3 and Wnk4
 Wnk1 antibody  >18 publications with SLC12A3 and Wnk1
 Products by Pathway  Pathway Diagram
 Cation-coupled Chloride Cotransporters Pathway antibodies  Cation-coupled Chloride Cotransporters Pathway Diagram
 SLC-mediated Transmembrane Transport Pathway antibodies  SLC-mediated Transmembrane Transport Pathway Diagram
 Transmembrane Transport Of Small Molecules Pathway antibodies  Transmembrane Transport Of Small Molecules Pathway Diagram
 Transport Of Inorganic Cations/anions And Amino Acids/oligopeptides Pathway antibodies  Transport Of Inorganic Cations/anions And Amino Acids/oligopeptides Pathway Diagram
Diseases associated with anti-SLC12A3 antibodyOrgans/Tissues associated with anti-SLC12A3 antibody
 Disease Name  Pubmed Publications
 Kidney Diseases Antibodies  >158 publications with SLC12A3 and Kidney Diseases
 Gitelman Syndrome Antibodies  >107 publications with SLC12A3 and Gitelman Syndrome
 Hypokalemia Antibodies  >98 publications with SLC12A3 and Hypokalemia
 Hypertension Antibodies  >76 publications with SLC12A3 and Hypertension
 Vascular Diseases Antibodies  >51 publications with SLC12A3 and Vascular Diseases
 Nervous System Diseases Antibodies  >20 publications with SLC12A3 and Nervous System Diseases
 Renal Insufficiency Antibodies  >11 publications with SLC12A3 and Renal Insufficiency
 Muscle Weakness Antibodies  >10 publications with SLC12A3 and Muscle Weakness
 Proteinuria Antibodies  >7 publications with SLC12A3 and Proteinuria
 Hypertrophy Antibodies  >6 publications with SLC12A3 and Hypertrophy
 Organ/Tissue Name  Pubmed Publications
 Kidney Antibodies  >174 publications with SLC12A3 and Kidney
 Brain Antibodies  >6 publications with SLC12A3 and Brain
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