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anti-NCK2 antibody :: Rabbit anti-Human NCK2 Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS300114
Immunohistochemistry (IHC)
Unit / Price
7 mL  /  $280 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

NCK2, Polyclonal Antibody

 Also Known As   

Rabbit Anti-Human NCK2 Polyclonal Antibody

 Product Gene Name   

anti-NCK2 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    604930
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 Clonality    Polyclonal
 Isotype    IgG
 Host    Rabbit
 Species Reactivity    Human
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 Form/Format    7.0 ml pre-diluted immunogen affinity purified rabbit polyclonal antibody in TBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide.
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 Immunogen    Recombinant protein encoding aa 119-276 of human NCK2.
 Control Tissue    Breast Carcinoma, Tonsil
 Cellular Localization    Cytoplasm
 Entrez Gene Code    8440
 Pathway    Signal Transduction
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 Preparation and Storage    Store at 2-8 degree C. Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. There are no definitive signs to indicate instability of this product; therefore, positive and negative controls should be tested simultaneously with unknown specimens. If unexpected results are observed which cannot be explained by variations in laboratory procedures and a problem with the reagent is suspected, contact Technical Support.
 Other Notes    Small volumes of anti-NCK2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Product Description specifically for anti-NCK2 antibody

   The NCK family of SH2/SH3 adaptor proteins consists of two members, NCK1 and NCK2, which couple tyrosine kinase signaling, including the EGF and PDGF receptor-pathways, to downstream signaling proteins. The NCK2 protein binds human PDGFR-b (Tyr-1009); overexpression of NCK2 inhibits PDGF-induced membrane ruffling and lamellipod formation.
 Applications Tested/Suitable for anti-NCK2 antibody   

Immunohistochemistry (IHC) Paraffin

 Application Notes for anti-NCK2 antibody    Immunohistochemistry Procedure
Specimen Preparation: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody. Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols. Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:100. The dilutions are estimates; actual results may differ because of variability in methods and protocols. Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min. Primary Antibody Incubation: Incubate for 10 minutes at room temperature. Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween. Visualization: Detect the antibody as instructed by the instructions provided with the visualization system.
Immunohistochemistry Dilution: Ready To Use
IHC Positive Control: Tonsil, Breast Carcinoma
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 Immunohistochemistry (IHC) of anti-NCK2 antibody    Human breast carcinoma stained with anti-NCK2 antibody
anti-NCK2 antibody Immunohistochemistry (IHC) (IHC) image
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NCBI/Uniprot data below describe general gene information for NCK2. It may not necessarily be applicable to this product.
 NCBI GI #    52630425
 NCBI GeneID    8440
 NCBI Accession #    NP_001004720.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001004720.2 [Other Products]
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 UniProt Secondary Accession #    Q9BWN9; Q9UIC3; D3DVK1 [Other Products]
 UniProt Related Accession #    O43639 [Other Products]
 Molecular Weight    47 kDa
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 NCBI Official Full Name    cytoplasmic protein NCK2 isoform A
 NCBI Official Synonym Full Names    NCK adaptor protein 2
 NCBI Official Symbol    NCK2 [Similar Products]
 NCBI Official Synonym Symbols   
GRB4; NCKbeta
[Similar Products]
 NCBI Protein Information    cytoplasmic protein NCK2; SH2/SH3 adaptor protein NCK-beta; growth factor receptor-bound protein 4; noncatalytic region of tyrosine kinase, beta
 UniProt Protein Name    Cytoplasmic protein NCK2
 UniProt Synonym Protein Names   
Growth factor receptor-bound protein 4; NCK adaptor protein 2; Nck-2; SH2/SH3 adaptor protein NCK-beta
 Protein Family    Cytoplasmic protein
 UniProt Gene Name    NCK2 [Similar Products]
 UniProt Synonym Gene Names    GRB4; Nck-2 [Similar Products]
 UniProt Entry Name    NCK2_HUMAN
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 NCBI Summary for NCK2    This gene encodes a member of the NCK family of adaptor proteins. The protein contains three SH3 domains and one SH2 domain. The protein has no known catalytic function but has been shown to bind and recruit various proteins involved in the regulation of receptor protein tyrosine kinases. It is through these regulatory activities that this protein is believed to be involved in cytoskeletal reorganization. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
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 UniProt Comments for NCK2    NCK2: Adapter protein which associates with tyrosine- phosphorylated growth factor receptors or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in ELK1- dependent transcriptional activation in response to activated Ras signaling.

Protein type: Adaptor/scaffold; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 2q12

Cellular Component: endoplasmic reticulum; cytoplasm; vesicle membrane; cytosol

Molecular Function: protein binding; receptor signaling complex scaffold activity; SH3/SH2 adaptor activity; cytoskeletal adaptor activity

Biological Process: lamellipodium biogenesis; epidermal growth factor receptor signaling pathway; axon guidance; cell migration; T cell activation; positive regulation of signal transduction; regulation of epidermal growth factor receptor activity; negative regulation of peptidyl-serine phosphorylation; signal complex assembly; actin filament organization; signal transduction; regulation of translation; negative regulation of cell proliferation; positive regulation of actin filament polymerization; ephrin receptor signaling pathway; positive regulation of transcription from RNA polymerase II promoter; positive regulation of T cell proliferation; vascular endothelial growth factor receptor signaling pathway
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 Research Articles on NCK2    1. Proteasomal degradation of Nck1 but not Nck2 regulates RhoA activation and actin dynamics.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-NCK2 antibodyPathways associated with anti-NCK2 antibody
 Reference Product  PubMed Publications
 ILK antibody  >5 publications with NCK2 and ILK
 LIMS1 antibody  >5 publications with NCK2 and LIMS1
 PAK1 antibody  >3 publications with NCK2 and PAK1
 PDGFRB antibody  >3 publications with NCK2 and PDGFRB
 RHOU antibody  >1 publications with NCK2 and RHOU
 EGFR antibody  >1 publications with NCK2 and EGFR
 Products by Pathway  Pathway Diagram
 Activation Of Rac Pathway antibodies  Activation Of Rac Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 BDNF Signaling Pathway antibodies  BDNF Signaling Pathway Diagram
 Cell-Cell Communication Pathway antibodies  Cell-Cell Communication Pathway Diagram
 Developmental Biology Pathway antibodies  Developmental Biology Pathway Diagram
 Downstream Signal Transduction Pathway antibodies  Downstream Signal Transduction Pathway Diagram
 EGFR1 Signaling Pathway antibodies  EGFR1 Signaling Pathway Diagram
 EPH-Ephrin Signaling Pathway antibodies  EPH-Ephrin Signaling Pathway Diagram
Diseases associated with anti-NCK2 antibodyOrgans/Tissues associated with anti-NCK2 antibody
 Disease Name  Pubmed Publications
 Hypertrophy Antibodies  >1 publications with NCK2 and Hypertrophy
 Heart Diseases Antibodies  >1 publications with NCK2 and Heart Diseases
 Cell Transformation, Neoplastic Antibodies  >1 publications with NCK2 and Cell Transformation, Neoplastic
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >6 publications with NCK2 and Brain
 Skin Antibodies  >4 publications with NCK2 and Skin
 Intestine Antibodies  >3 publications with NCK2 and Intestine
 Heart Antibodies  >3 publications with NCK2 and Heart
 Embryonic Tissue Antibodies  >2 publications with NCK2 and Embryonic Tissue
 Kidney Antibodies  >2 publications with NCK2 and Kidney
 Mammary Gland Antibodies  >1 publications with NCK2 and Mammary Gland
 Lung Antibodies  >1 publications with NCK2 and Lung
 Uterus Antibodies  >1 publications with NCK2 and Uterus
 Eye Antibodies  >1 publications with NCK2 and Eye
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