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anti-NEFM antibody :: Goat NEFM/NF-M Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS421706
Western Blot (WB)
Unit / Price
0.1 mg  /  $300 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

NEFM/NF-M, Polyclonal Antibody

★Popular Item★
 Also Known As   

Goat anti-NEFM/NF-M Antibody

 Product Synonym Names    NEFM; neurofilament, medium polypeptide; NEF3; NF-M; NFM; neurofilament triplet M protein; neurofilament, medium polypeptide 150kDa; neurofilament-3 (150 kD medium); NEFM antibody; neurofilament; medium polypeptide antibody; NEF3 antibody; NF-M antibody; NFM antibody; neurofilament triplet M protein antibody; neurofilament; medium polypeptide 150kDa antibody; neurofilament-3 (150 kD medium) antibody; NEFM / NF-M
 Product Gene Name   

anti-NEFM antibody

[Similar Products]
 Product Synonym Gene Name    NF-M [Similar Products]
 Antibody/Peptide Pairs    NEFM / NF-M peptide (MBS426551) is used for blocking the activity of NEFM/NF-M antibody (MBS421706)
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Immunogen Sequence    QASHITVERK DYLK
 OMIM    162250
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 Clonality    Polyclonal
 Host    Goat
 Species Reactivity    Tested: Mouse; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
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 Purity/Purification    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
 Form/Format    Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
 Concentration    100ug specific antibody in 200ul (lot specific)
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 Immunogen    Peptide with sequence C-QASHITVERKDYLK, from the internal region of the protein sequence according to NP_005373.2; NP_001099011.1.
 Epitope    Internal region
 Note    This antibody is expected to recognize both reported isoforms (NP_005373.2; NP_001099011.1).
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 Preparation and Storage    Aliquot and store at -20 degree C. Minimize freezing and thawing.
 ISO Certification    Manufactured in an ISO 9001:2015 Certified Laboratory.
 Other Notes    Small volumes of anti-NEFM antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Applications Tested/Suitable for anti-NEFM antibody   

Peptide ELISA (EIA), Western Blot (WB)

 Application Notes for anti-NEFM antibody    Peptide ELISA: Antibody detection limit dilution 1: 128000.
Western Blot: Approx 170-200kDa band observed in Mouse Brain lysates (calculated MW of 102kDa according to NP_005373.2). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0. 03-0.1ug/ml.
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 Western Blot (WB) of anti-NEFM antibody    (0.03ug/ml) staining of Mouse Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
anti-NEFM antibody Western Blot (WB) (WB) image
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NCBI/Uniprot data below describe general gene information for NEFM. It may not necessarily be applicable to this product.
 NCBI GI #    157738649
 NCBI GeneID    4741
 NCBI Accession #    NP_005373.2 [Other Products]
 NCBI Related Accession #    NP_001099011.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_005382.2 [Other Products]
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 UniProt Secondary Accession #    Q4QRK6; B4DGN2; E9PBF7 [Other Products]
 UniProt Related Accession #    P07197 [Other Products]
 Molecular Weight    59,468 Da
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 NCBI Official Full Name    neurofilament medium polypeptide isoform 1
 NCBI Official Synonym Full Names    neurofilament, medium polypeptide
 NCBI Official Symbol    NEFM [Similar Products]
 NCBI Official Synonym Symbols   
NFM; NEF3; NF-M
[Similar Products]
 NCBI Protein Information    neurofilament medium polypeptide
 UniProt Protein Name    Neurofilament medium polypeptide
 UniProt Synonym Protein Names   
160 kDa neurofilament protein; Neurofilament 3; Neurofilament triplet M protein
 Protein Family    Neurofilament medium polypeptide
 UniProt Gene Name    NEFM [Similar Products]
 UniProt Synonym Gene Names    NEF3; NFM; NF-M [Similar Products]
 UniProt Entry Name    NFM_HUMAN
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 NCBI Summary for NEFM    Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the medium neurofilament protein. This protein is commonly used as a biomarker of neuronal damage. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008]
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 UniProt Comments for NEFM    NFM: a neurofilament intermediate filament protein. Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. The levels of phosphorylation are altered developmentally and coincident with a change in the neurofilament function.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 8p21

Cellular Component: axon; neurofilament

Molecular Function: microtubule binding; protein binding; structural constituent of cytoskeleton

Biological Process: neurofilament bundle assembly
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 Research Articles on NEFM    1. There was a strongly elevated reactivity against neurofilament medium in a subset of schizophrenia patients compared to controls.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with anti-NEFM antibodyDiseases associated with anti-NEFM antibody
 Products by Pathway  Pathway Diagram
 Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies  Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
 Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies  Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
 Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies  Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >2 publications with NEFM and Nervous System Diseases
 Brain Diseases Antibodies  >1 publications with NEFM and Brain Diseases
 Breast Neoplasms Antibodies  >1 publications with NEFM and Breast Neoplasms
Organs/Tissues associated with anti-NEFM antibody
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >5 publications with NEFM and Brain
 Nerve Antibodies  >3 publications with NEFM and Nerve
 Embryonic Tissue Antibodies  >2 publications with NEFM and Embryonic Tissue
 Prostate Antibodies  >1 publications with NEFM and Prostate
 Pancreas Antibodies  >1 publications with NEFM and Pancreas
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