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anti-NELFA antibody :: Mouse anti-Human NELFA Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS6001042
Unit / Price
0.05 mg  /  $580 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

NELFA, Polyclonal Antibody

★Popular Item★
 Also Known As   

NELFA (Negative Elongation Factor A, NELF-A, Wolf-Hirschhorn Syndrome Candidate 2 Protein, WHSC2, P/OKcl.15)

 Product Synonym Names    Anti -NELFA (Negative Elongation Factor A, NELF-A, Wolf-Hirschhorn Syndrome Candidate 2 Protein, WHSC2, P/OKcl.15)
 Product Gene Name   

anti-NELFA antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Immunogen Sequence    MASMRESDTG LWLHNKLGAT DELWAPPSIA SLLTAAVIDN IRLCFHGLSS AVKLKLLLGT LHLPRRTVDE MKGALMEIIQ LASLDSDPWV LMVADILKSF PDTGSLNLEL EEQNPNVQDI LGELREKVGE CEASAMLPLE CQYLNKNALT TLAGPLTPPV KHFQLKRKPK SATLRAELLQ KSTETAQQLK RSAGVPFHAK GRGLLRKMDT TTPLKGIPKQ APFRSPTAPS VFSPTGNRTP IPPSRTLLRK ERGVKLLDIS ELDMVGAGRE AKRRRKTLDA EVVEKPAKEE TVVENATPDY AAGLVSTQKL GSLNNEPALP STSYLPSTPS VVPASSYIPS SETPPAPSSR EASRPPEEPS APSPTLPAQF KQRAPMYNSG LSPATPTPAA PTSPLTPTTP PAVAPTTQTP PVAMVAPQTQ APAQQQPKKN LSLTREQMFA AQEMFKTANK VTRPEKALIL GFMAGSRENP CQEQGDVIQI KLSEHTEDLP KADGQGSTTM LVDTVFEMNY ATGQWTRFKK YKPMTNVS
 Chromosome Location    Chromosome: 4; NC_000004.11 (1984441..2010962, complement). Location: 4p16.3
 OMIM    606026
 3D Structure    ModBase 3D Structure for Q9H3P2
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 Clonality    Polyclonal
 Isotype    IgG
 Host    Mouse
 Species Reactivity    Human
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 Specificity    Recognizes human WHSC2.
 Purity/Purification    Affinity Purified
Purified by Protein A affinity chromatography.
 Form/Format    Supplied as a liquid in PBS, pH 7.4.
 Concentration    1mg/ml (lot specific)
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 Immunogen    Full length human WHSC2, aa1-528 (NP_005654.2).
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 Preparation and Storage    May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
 Other Notes    Small volumes of anti-NELFA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Product Categories/Family for anti-NELFA antibody    Antibodies; Abs to Disease Markers
 Applications Tested/Suitable for anti-NELFA antibody   

Western Blot (WB), Immunofluorescence (IF)

 Application Notes for anti-NELFA antibody    Suitable for use in Immunofluorescence and Western Blot.
Dilution: Immunofluorescence: 10ug/ml
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 Western Blot of anti-NELFA antibody    WHSC2 polyclonal antibody Western Blot analysis of WHSC2 expression in Jurkat.
anti-NELFA antibody Western Blot (WB) image
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 Western Blot of anti-NELFA antibody    Western Blot analysis of WHSC2 expression in transfected 293T cell line by WHSC2 polyclonal antibody. Lane 1: WHSC2 transfected lysate (58.08kD). Lane 2: Non-transfected lysate.
anti-NELFA antibody Western Blot (WB) image
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 Immunofluorescence of anti-NELFA antibody    Immunofluorescence of purified antibody to WHSC2 on HeLa cell. (Concentration 10ug/ml).
anti-NELFA antibody Immunofluorescence image
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NCBI/Uniprot data below describe general gene information for NELFA. It may not necessarily be applicable to this product.
 NCBI GI #    212276499
 NCBI GeneID    7469
 NCBI Accession #    Q9H3P2.3 [Other Products]
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 UniProt Primary Accession #    Q9H3P2 [Other Products]
 UniProt Secondary Accession #    O95392; A2A2T1 [Other Products]
 UniProt Related Accession #    Q9H3P2 [Other Products]
 Molecular Weight    57,277 Da [Similar Products]
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 NCBI Official Full Name    Negative elongation factor A
 NCBI Official Synonym Full Names    negative elongation factor complex member A
 NCBI Official Symbol    NELFA [Similar Products]
 NCBI Official Synonym Symbols   
WHSC2; NELF-A; P/OKcl.15
[Similar Products]
 NCBI Protein Information    negative elongation factor A; wolf-Hirschhorn syndrome candidate 2 protein
 UniProt Protein Name    Negative elongation factor A
 UniProt Synonym Protein Names   
Wolf-Hirschhorn syndrome candidate 2 protein
 UniProt Gene Name    NELFA [Similar Products]
 UniProt Synonym Gene Names    WHSC2; NELF-A [Similar Products]
 UniProt Entry Name    NELFA_HUMAN
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 NCBI Summary for NELFA    This gene is expressed ubiquitously with higher levels in fetal than in adult tissues. It encodes a protein sharing 93% sequence identity with the mouse protein. Wolf-Hirschhorn syndrome (WHS) is a malformation syndrome associated with a hemizygous deletion of the distal short arm of chromosome 4. This gene is mapped to the 165 kb WHS critical region, and may play a role in the phenotype of the WHS or Pitt-Rogers-Danks syndrome. The encoded protein is found to be capable of reacting with HLA-A2-restricted and tumor-specific cytotoxic T lymphocytes, suggesting a target for use in specific immunotherapy for a large number of cancer patients. This protein has also been shown to be a member of the NELF (negative elongation factor) protein complex that participates in the regulation of RNA polymerase II transcription elongation. [provided by RefSeq, Jul 2008]
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 UniProt Comments for NELFA    WHSC2: Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. Probably required to interact with the RNA polymerase II complex. Belongs to the NELF-A family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 4p16.3

Cellular Component: nucleoplasm; cytoplasm

Molecular Function: protein binding; chromatin binding

Biological Process: transcription from RNA polymerase II promoter; viral reproduction; positive regulation of viral transcription; multicellular organismal development; RNA elongation from RNA polymerase II promoter; gene expression
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 Research Articles on NELFA    1. data show that NELF subunits exhibit highly specific subcellular localizations, such as in NELF bodies or in midbodies, and some shuttle actively between the nucleus and cytoplasm; loss of NELF from cells can lead to enlarged and/or multiple nuclei
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with anti-NELFA antibody
 Products by Pathway  Pathway Diagram
 Abortive Elongation Of HIV-1 Transcript In The Absence Of Tat Pathway antibodies  Abortive Elongation Of HIV-1 Transcript In The Absence Of Tat Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Formation Of HIV Elongation Complex In The Absence Of HIV Tat Pathway antibodies  Formation Of HIV Elongation Complex In The Absence Of HIV Tat Pathway Diagram
 Formation Of HIV-1 Elongation Complex Containing HIV-1 Tat Pathway antibodies  Formation Of HIV-1 Elongation Complex Containing HIV-1 Tat Pathway Diagram
 Formation Of RNA Pol II Elongation Complex Pathway antibodies  Formation Of RNA Pol II Elongation Complex Pathway Diagram
 Formation Of The Early Elongation Complex Pathway antibodies  Formation Of The Early Elongation Complex Pathway Diagram
 Formation Of The HIV-1 Early Elongation Complex Pathway antibodies  Formation Of The HIV-1 Early Elongation Complex Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 HIV Infection Pathway antibodies  HIV Infection Pathway Diagram
 HIV Life Cycle Pathway antibodies  HIV Life Cycle Pathway Diagram
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