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anti-RELA antibody :: Rabbit NF-kappaB p65 Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS8213602
Unit / Price
0.03 mL  /  $165 +1 FREE 8GB USB
0.1 mL  /  $245 +1 FREE 8GB USB
0.2 mL  /  $350 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

NF-kappaB p65 (RELA), Polyclonal Antibody

★Popular Item★
 Also Known As   

Anti-NF-kappaB p65 (AcK310) Antibody

 Product Synonym Names    NFKB3; Transcription factor p65; Nuclear factor NF-kappa-B p65 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3
 Product Gene Name   

anti-RELA antibody

[Similar Products]
 Antibody/Peptide Pairs    NF-kappaB p65 peptide (MBS826929) is used for blocking the activity of NF-kappaB p65 antibody (MBS8213602)
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    164014
 3D Structure    ModBase 3D Structure for Q04206
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 Clonality    Polyclonal
 Host    Rabbit
 Species Reactivity    Human, Mouse, Rat, Bovine, Porcine
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 Specificity    Recognizes endogenous levels of NF-kappaB p65 (AcK310) protein.
 Purity/Purification    The antibody was purified by immunogen affinity chromatography.
 Form/Format    Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
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 Immunogen    KLH-conjugated synthetic peptide encompassing a sequence within the center region of human NF-kappaB p65. The exact sequence is proprietary.
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 Preparation and Storage    Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
 Other Notes    Small volumes of anti-RELA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-RELA antibody

   Rabbit polyclonal antibody to NF-kappaB p65 (AcK310)
 Applications Tested/Suitable for anti-RELA antibody   

Western Blot (WB),Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Chromatin Immunoprecipitation (ChIP)

 Application Notes for anti-RELA antibody    WB (1/500 - 1/1000), IHC (1/100 - 1/200), IF (1/100 - 1/500), ICC (1/100 - 1/500), ChIP (1/10 - 1/100)
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 Western Blot (WB) of anti-RELA antibody    Western blot analysis of NF-kappaB p65 (AcK310) expression in HeLa (A), mouse lung (B) whole cell lysates.
anti-RELA antibody Western Blot (WB) (WB) image
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 Immunohistochemistry (IHC) of anti-RELA antibody    Immunohistochemical analysis of NF-kappaB p65 (AcK310) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
anti-RELA antibody Immunohistochemistry (IHC) (IHC) image
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 Immunofluorescence (IF) of anti-RELA antibody    Immunofluorescent analysis of NF-kappaB p65 (AcK310) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 degree C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
anti-RELA antibody Immunofluorescence (IF) image
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NCBI/Uniprot data below describe general gene information for RELA. It may not necessarily be applicable to this product.
 NCBI GI #    223468681
 NCBI GeneID    5970
 NCBI Accession #    NP_001138610.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001145138.1 [Other Products]
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 UniProt Primary Accession #    Q04206 [Other Products]
 UniProt Secondary Accession #    Q6GTV1; Q6SLK1 [Other Products]
 UniProt Related Accession #    Q04206 [Other Products]
 Molecular Weight    59,910 Da
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 NCBI Official Full Name    transcription factor p65 isoform 2
 NCBI Official Synonym Full Names    v-rel avian reticuloendotheliosis viral oncogene homolog A
 NCBI Official Symbol    RELA [Similar Products]
 NCBI Official Synonym Symbols   
p65; NFKB3
[Similar Products]
 NCBI Protein Information    transcription factor p65
 UniProt Protein Name    Transcription factor p65
 UniProt Synonym Protein Names   
Nuclear factor NF-kappa-B p65 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3
 Protein Family    Transcription factor
 UniProt Gene Name    RELA [Similar Products]
 UniProt Synonym Gene Names    NFKB3 [Similar Products]
 UniProt Entry Name    TF65_HUMAN
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 NCBI Summary for RELA    NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]
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 UniProt Comments for RELA    NFkB-p65: a subunit of NF-kappa-B transcription complex, which plays a crucial role in inflammatory and immune responses. The inhibitory effect of I-kappa-B upon NF-kappa-B in the cytoplasm is exerted primarily through the interaction with p65. P65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. There are five NFkB proteins in mammals (RelA/NFkB-p65, RelB, c-Rel, NF-_B1/NFkB-p105, and NF-_B2/NFkB-p100). They form a variety of homodimers and heterodimers, each of which activates its own characteristic set of genes. Three splice-variant isoforms have been identified.

Protein type: Transcription factor; Nuclear receptor co-regulator; DNA-binding

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: nucleoplasm; transcription factor complex; cytoplasm; cytosol; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; identical protein binding; transcription activator binding; protein N-terminus binding; protein kinase binding; transcription factor binding; NF-kappaB binding; protein binding; DNA binding; protein heterodimerization activity; ubiquitin protein ligase binding; protein complex binding; chromatin binding; phosphate binding; transcription factor activity

Biological Process: nerve growth factor receptor signaling pathway; viral reproduction; positive regulation of transcription, DNA-dependent; negative regulation of insulin receptor signaling pathway; toll-like receptor 3 signaling pathway; T cell receptor signaling pathway; activation of NF-kappaB transcription factor; toll-like receptor 5 signaling pathway; hair follicle development; defense response to virus; toll-like receptor 4 signaling pathway; response to drug; membrane protein intracellular domain proteolysis; positive regulation of I-kappaB kinase/NF-kappaB cascade; acetaldehyde metabolic process; response to amino acid stimulus; toll-like receptor 2 signaling pathway; organ morphogenesis; positive regulation of chondrocyte differentiation; response to muramyl dipeptide; response to mechanical stimulus; positive regulation of transcription from RNA polymerase II promoter; toll-like receptor 9 signaling pathway; response to progesterone stimulus; negative regulation of protein catabolic process; negative regulation of transcription, DNA-dependent; negative regulation of apoptosis; transcription from RNA polymerase II promoter; response to cAMP; response to morphine; negative regulation of transcription from RNA polymerase II promoter; toll-like receptor 10 signaling pathway; response to insulin stimulus; response to organic substance; positive regulation of cell proliferation; positive regulation of interferon type I production; inflammatory response; aging; positive regulation of Schwann cell differentiation; MyD88-independent toll-like receptor signaling pathway; cytokine and chemokine mediated signaling pathway; liver development; response to UV-B; MyD88-dependent toll-like receptor signaling pathway; positive regulation of interleukin-12 biosynthetic process; regulation of inflammatory response; toll-like receptor signaling pathway; innate immune response; cellular defense response; response to cobalamin
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 Research Articles on RELA    1. Suggest that uromodullin may have a renal physiologic function related to its inhibitory effect on the NFkappaB pathway.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-RELA antibodyPathways associated with anti-RELA antibody
 Reference Product  PubMed Publications
 IKBKB antibody  >553 publications with RELA and IKBKB
 CHUK antibody  >160 publications with RELA and CHUK
 NFKB1 antibody  >129 publications with RELA and NFKB1
 HDAC1 antibody  >41 publications with RELA and HDAC1
 CREBBP antibody  >36 publications with RELA and CREBBP
 EP300 antibody  >29 publications with RELA and EP300
 HDAC2 antibody  >19 publications with RELA and HDAC2
 NFKBIA antibody  >17 publications with RELA and NFKBIA
 NFKBIB antibody  >4 publications with RELA and NFKBIB
 UBC antibody  >2 publications with RELA and UBC
 Products by Pathway  Pathway Diagram
 AGE/RAGE Pathway antibodies  AGE/RAGE Pathway Diagram
 Activated TLR4 Signalling Pathway antibodies  Activated TLR4 Signalling Pathway Diagram
 Activation Of NF-kappaB In B Cells Pathway antibodies  Activation Of NF-kappaB In B Cells Pathway Diagram
 Acute Myeloid Leukemia Pathway antibodies  Acute Myeloid Leukemia Pathway Diagram
 Acute Myeloid Leukemia Pathway antibodies  Acute Myeloid Leukemia Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Adipocytokine Signaling Pathway antibodies  Adipocytokine Signaling Pathway Diagram
 Adipocytokine Signaling Pathway antibodies  Adipocytokine Signaling Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
Diseases associated with anti-RELA antibodyOrgans/Tissues associated with anti-RELA antibody
 Disease Name  Pubmed Publications
 Necrosis Antibodies  >1671 publications with RELA and Necrosis
 Inflammation Antibodies  >1472 publications with RELA and Inflammation
 Liver Diseases Antibodies  >312 publications with RELA and Liver Diseases
 Nervous System Diseases Antibodies  >261 publications with RELA and Nervous System Diseases
 Breast Neoplasms Antibodies  >139 publications with RELA and Breast Neoplasms
 Kidney Diseases Antibodies  >135 publications with RELA and Kidney Diseases
 Neoplasms, Experimental Antibodies  >122 publications with RELA and Neoplasms, Experimental
 Fibrosis Antibodies  >119 publications with RELA and Fibrosis
 Cell Transformation, Neoplastic Antibodies  >106 publications with RELA and Cell Transformation, Neoplastic
 Heart Diseases Antibodies  >101 publications with RELA and Heart Diseases
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >1258 publications with RELA and Blood
 Liver Antibodies  >619 publications with RELA and Liver
 Vascular Antibodies  >574 publications with RELA and Vascular
 Lung Antibodies  >470 publications with RELA and Lung
 Brain Antibodies  >334 publications with RELA and Brain
 Muscle Antibodies  >316 publications with RELA and Muscle
 Bone Antibodies  >254 publications with RELA and Bone
 Kidney Antibodies  >228 publications with RELA and Kidney
 Heart Antibodies  >205 publications with RELA and Heart
 Intestine Antibodies  >197 publications with RELA and Intestine
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