Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource rightarrow Antibody rightarrow NFKB2 rightarrow Polyclonal NFKB2  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Menu separator
separator
ELISA Kit ELISA Kit
CLIA Kit CLIA Kit
PCR Kit PCR Kit
Monoclonal Antibody Monoclonal Antibody
Polyclonal Antibody Polyclonal Antibody
Secondary Antibody Secondary Antibody
Antigen Antigen
Biochemical Biochemical
cDNA Clone cDNA Clone
siRNA siRNA
Peptide Peptide
Recombinant/Purified Protein Rec./Purified Protein

Custom ELISA Kit Custom ELISA Kit
Custom Protein Custom Protein
Custom Antibody Custom Antibody
Antibody Matched Pairs Antibody Matched Pairs
Antibody & Corresponding Blocking Peptide Pairs Antibody Peptide Pairs
Phospho-Specific Antibodies Phospho Antibodies
Products by Disease Products by Disease
Products by Pathway Products by Pathway
Products by Tissue Products by Tissue

arrow Advanced Search
arrow Submit Technical Q&A
arrow International Distributors
arrow Contact Us
separator
Our Best Sellers moreseparator
separator
 • Podocin (PDCN) ELISA Kit
 • Interleukin 1 beta, aa118-269, His tag (il1b) Recombinant Protein
 • IgG receptor FcRn large subunit p51 Antibody
 • ACTG2 Antibody
 • QDPR Antibody
 • POT1 Antibody
 • Microphthalmia Transcription Factor (MiTF) Antibody
 • UDP-glucuronosyltransferase 1-1 (UGT1A1) Recombinant Protein
 • Adenylyl Cyclase Associated Protein 1 (CAP1) ELISA Kit
 • Ovalbumin, Denatured (SERPINB14) Antibody
 • IFN beta Antibody
 • E Coli K99 pili Antibody
 • BCL-2 Antibody
 • Bone Morphogenetic Protein Receptor 1A (BMPR1A) ELISA Kit
 downarrow more ...
separator
separator
DatasheetFull DatasheetPrinter Friendly DatasheetPrint This DatasheetAdd to Compare ListHave Questions? Ask UsRequest for a Quotation today

anti-NFKB2 antibody :: Rabbit anti-Human NFkB p52 Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS621489 anti-NFKB2 antibody
Unit / Price
0.1 mL  /  $620 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

NFkB p52 (NFKB2), Polyclonal Antibody

 Also Known As   

NFkB p52 (NF Kappa B, Nuclear Factor kappa B)

 Product Synonym Names    Anti -NFkB p52 (NF Kappa B, Nuclear Factor kappa B)
 Product Gene Name   

anti-NFKB2 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
Table BarTOPTable Bar
 Chromosome Location    Chromosome: 10; NC_000010.10 (104153867..104162286). Location: 10q24
 OMIM    164012
 3D Structure    ModBase 3D Structure for Q00653
Section Bar
 Clonality    Polyclonal
 Isotype    IgG
 Host    Rabbit
 Species Reactivity    Human
Section Bar
 Specificity    Recognizes human NF KB, p52, (52kD) and its 110kD precursor.
 Purity/Purification    Affinity Purified
Purified by Protein A affinity chromatography.
 Form/Format    Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 150 mM NaCl, with 0.05% sodium azide.
Section Bar
 Immunogen    Synthetic peptide corresponding to amino acids located at the N-terminal region of human NF-kB, p52.
Section Bar
 Preparation and Storage    May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50), aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
 Other Notes    Small volumes of anti-NFKB2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Table BarTOPTable Bar
 

Product Description specifically for anti-NFKB2 antibody

   p52 is a subunit of the nuclear factor NF Kappa-B, which binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions.

The transcription factor NFkB is widely recognized as a critical mediator of immune and inflammatory responses. In most cell types, NFkB is found in the cytoplasm where it is associated with an inhibitory protein known as IkB. An impressive variety of stimuli (tumor necrosis factor, interleukin 1, T-cell activation signals, bacterial endotoxins, viral transforming proteins, certain growth factors and reactive oxygen intermediates) lead to the rapid nuclear accumulation of NFkB by the induced phosphorylation and subsequent degradation of IkB. In the nucleus, NFkB regulates genes encoding cytokines, cytokine receptors, cell adhesion molecules, proteins involved in coagulation and genes involved in cell growth control. Additionally, NFkB is thought to be an important transcriptional regulator for HIV. Growing evidence indicates that the dysregulation of NFkB may be key to a number of diseases including arthritis and other inflammatory diseases, Alzheimer's disease, atherosclerosis and cancer.
NFkB is formed through the association of multiple subunits, either as a homodimer or heterodimer. Subunits have been identified as p50 (NFkB1), p65 (RelA), c-Rel, RelB and p52 (NFkB2). The classic NFkB form exists as a p50-p65 heterodimer and predominates in many cell types. Many of the possible combinatorial forms of homo- and heterodimers have been identified and growing evidence indicates that different forms of NFkB have different functions in cells. Interestingly, both the p50 and p52 subunits are derived from the precursor proteins p105 and p100 respectively, that each contain multiple copies of the so-called ankyrin repeat at their C-termini. Nuclear translocation of NFkB is confirmed by the use of electrophorectic mobility shift assays or by immunoblotting with nuclear extracts. The subunit composition of NFkB is confirmed by the use of antibodies that "supershift" the DNA/protein complex.
 Product Categories/Family for anti-NFKB2 antibody    Antibodies; Abs to Transcription Factors
 Applications Tested/Suitable for anti-NFKB2 antibody   

Western Blot (WB), Immunoprecipitation (IP)

 Application Notes for anti-NFKB2 antibody    Suitable for use in Immunoprecipitation and Western Blot.
Dilution: Western Blot: 1:1000 dilution detected NF KB, p52 and its 110kDa precursor in RIPA lysates from Raji cells. Raji cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-NF KB, p52 (1:1000). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
Immunoprecipitation: 4ul immunoprecipitated NF KB, p52 and its 110kDa precursor from 500ug of a Raji RIPA cell lysate.
Table BarTOPTable Bar
NCBI/Uniprot data below describe general gene information for NFKB2. It may not necessarily be applicable to this product.
 NCBI GI #    387157878
 NCBI GeneID    4791
 NCBI Accession #    NP_001248332.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001261403.1 [Other Products]
Section Bar
 UniProt Primary Accession #    Q00653 [Other Products]
 UniProt Secondary Accession #    Q04860; Q9BU75; Q9H471; Q9H472; A8K9D9; D3DR83 [Other Products]
 UniProt Related Accession #    Q00653; AAB23437 [Other Products]
 Molecular Weight    96,749 Da [Similar Products]
Table BarTOPTable Bar
 NCBI Official Full Name    nuclear factor NF-kappa-B p100 subunit isoform b
 NCBI Official Synonym Full Names    nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (p49/p100)
 NCBI Official Symbol    NFKB2 [Similar Products]
 NCBI Official Synonym Symbols   
p52; p105; H2TF1; LYT10; LYT-10; NF-kB2
[Similar Products]
 NCBI Protein Information    nuclear factor NF-kappa-B p100 subunit; oncogene Lyt-10; DNA-binding factor KBF2; lymphocyte translocation chromosome 10 protein; nuclear factor of kappa light chain gene enhancer in B-cells 2
 UniProt Protein Name    Nuclear factor NF-kappa-B p100 subunit
 UniProt Synonym Protein Names   
DNA-binding factor KBF2; H2TF1; Lymphocyte translocation chromosome 10 protein; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 2; Oncogene Lyt-10
 Protein Family    Nuclear factor
 UniProt Gene Name    NFKB2 [Similar Products]
 UniProt Synonym Gene Names    LYT10; Lyt10 [Similar Products]
 UniProt Entry Name    NFKB2_HUMAN
Section Bar
 NCBI Summary for NFKB2    This gene encodes one of the subunits of the transcription factor complex nuclear factor-kappa-B (NFkB). The NFkB transcription factor complex is expressed in numerous cell types and functions as a central activator of genes involved in inflammation and immune function. The NFkB complex can consist of different subunits that form both homo- or heterodimers which bind specific kappa-B elements in target genes. This gene encodes the p100 subunit that is processed into the active p52 subunit. This protein can function as both a transcriptional activator and repressor, depending on its dimer partner. Alternate splicing results in both coding and non-coding variants. [provided by RefSeq, May 2012]
Section Bar
 UniProt Comments for NFKB2    NFkB-p100: a transcription factor of the nuclear factor-kappaB ( NFkB) group. Precursor of the p52 subunit of the nuclear factor NF-kappa-B, which binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. The precursor protein itself does not bind to DNA. LT-beta receptor agonists and LPS induce NF-kappaB/p100 processing to p52 at the level of the ribosome. There are five NFkB proteins in mammals (RelA/NFkB-p65, RelB, c-Rel, NF-_B1/NFkB-p105, and NF-_B2/NFkB-p100). They form a variety of homodimers and heterodimers, each of which activates its own characteristic set of genes. Two spliced isoforms have been identified. Isoform p49 is a subunit of the NF-kappa-B protein complex, which stimulates the HIV enhancer in synergy with p65.

Protein type: Transcription factor; Oncoprotein; DNA-binding

Chromosomal Location of Human Ortholog: 10q24

Cellular Component: Bcl3/NF-kappaB2 complex; nucleoplasm; cytoplasm; nucleus; cytosol

Molecular Function: protein binding; transcription coactivator activity; chromatin binding; transcription factor activity

Biological Process: spleen development; I-kappaB kinase/NF-kappaB cascade; extracellular matrix organization and biogenesis; transcription, DNA-dependent; MyD88-independent toll-like receptor signaling pathway; follicular dendritic cell differentiation; rhythmic process; negative regulation of transcription from RNA polymerase II promoter; toll-like receptor 3 signaling pathway; toll-like receptor 2 signaling pathway; activation of NF-kappaB transcription factor; toll-like receptor 10 signaling pathway; toll-like receptor 5 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; regulation of transcription, DNA-dependent; response to cytokine stimulus; toll-like receptor signaling pathway; germinal center formation; positive regulation of interferon type I production; innate immune response; positive regulation of transcription from RNA polymerase II promoter; toll-like receptor 9 signaling pathway; inflammatory response; toll-like receptor 4 signaling pathway

Disease: Immunodeficiency, Common Variable, 10
Table BarTOPTable Bar
 Research Articles on NFKB2    1. TRAF2/NIK/NF-kappaB2 pathway regulates pancreatic ductal adenocarcinoma cell tumorigenicity
Table BarTOPTable Bar
 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Table BarTOPTable Bar
Products associated with anti-NFKB2 antibodyPathways associated with anti-NFKB2 antibody
 Reference Product  PubMed Publications
 RELB antibody  >91 publications with NFKB2 and RELB
 RELA antibody  >90 publications with NFKB2 and RELA
 IKBKB antibody  >53 publications with NFKB2 and IKBKB
 NFKB1 antibody  >49 publications with NFKB2 and NFKB1
 CHUK antibody  >18 publications with NFKB2 and CHUK
 BCL3 antibody  >11 publications with NFKB2 and BCL3
 IKBKG antibody  >3 publications with NFKB2 and IKBKG
 MAP3K14 antibody  >2 publications with NFKB2 and MAP3K14
 Products by Pathway  Pathway Diagram
 Activated TLR4 Signalling Pathway antibodies  Activated TLR4 Signalling Pathway Diagram
 Alternative NF-kappaB Pathway antibodies  Alternative NF-kappaB Pathway Diagram
 Cytokine Signaling In Immune System Pathway antibodies  Cytokine Signaling In Immune System Pathway Diagram
 Cytosolic Sensors Of Pathogen-associated DNA Pathway antibodies  Cytosolic Sensors Of Pathogen-associated DNA Pathway Diagram
 DEx/H-box Helicases Activate Type I IFN And Inflammatory Cytokines Production Pathway antibodies  DEx/H-box Helicases Activate Type I IFN And Inflammatory Cytokines Production Pathway Diagram
 DNA Damage Response (only ATM Dependent) Pathway antibodies  DNA Damage Response (only ATM Dependent) Pathway Diagram
 EBV LMP1 Signaling Pathway antibodies  EBV LMP1 Signaling Pathway Diagram
 Epstein-Barr Virus Infection Pathway antibodies  Epstein-Barr Virus Infection Pathway Diagram
 Epstein-Barr Virus Infection Pathway antibodies  Epstein-Barr Virus Infection Pathway Diagram
 Folate Metabolism Pathway antibodies  Folate Metabolism Pathway Diagram
Diseases associated with anti-NFKB2 antibodyOrgans/Tissues associated with anti-NFKB2 antibody
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >101 publications with NFKB2 and Neoplasms
 Necrosis Antibodies  >69 publications with NFKB2 and Necrosis
 Immune System Diseases Antibodies  >54 publications with NFKB2 and Immune System Diseases
 Inflammation Antibodies  >48 publications with NFKB2 and Inflammation
 Cell Transformation, Neoplastic Antibodies  >17 publications with NFKB2 and Cell Transformation, Neoplastic
 Cardiovascular Diseases Antibodies  >13 publications with NFKB2 and Cardiovascular Diseases
 Breast Neoplasms Antibodies  >13 publications with NFKB2 and Breast Neoplasms
 Lung Diseases Antibodies  >10 publications with NFKB2 and Lung Diseases
 Neoplasms, Experimental Antibodies  >8 publications with NFKB2 and Neoplasms, Experimental
 Liver Diseases Antibodies  >6 publications with NFKB2 and Liver Diseases
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >90 publications with NFKB2 and Blood
 Bone Antibodies  >34 publications with NFKB2 and Bone
 Bone Marrow Antibodies  >24 publications with NFKB2 and Bone Marrow
 Spleen Antibodies  >15 publications with NFKB2 and Spleen
 Lung Antibodies  >14 publications with NFKB2 and Lung
 Lymph Node Antibodies  >12 publications with NFKB2 and Lymph Node
 Lymph Antibodies  >11 publications with NFKB2 and Lymph
 Brain Antibodies  >11 publications with NFKB2 and Brain
 Kidney Antibodies  >10 publications with NFKB2 and Kidney
 Prostate Antibodies  >10 publications with NFKB2 and Prostate
Table BarTOPTable Bar
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions