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anti-Slc12a1 antibody :: Rabbit NKCC2 Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS805913
Western Blot (WB)
Unit / Price
0.1 mg  /  $435 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

NKCC2 (Slc12a1), Polyclonal Antibody

★Popular Item★
 Also Known As   

NKCC2 Antibody: HRP

 Product Synonym Names    SLCl2A1 Antibody, sodium potassium chloride cotransporter 2 Antibody, sodium-potassium-chloride cotransporter isoform 2 Antibody, BSCl Antibody, MBSCl Antibody, Na-K-2CI cotransporter Antibody, NKCC2A variant A Antibody, RBSC Antibody, Antibody
 Product Gene Name   

anti-Slc12a1 antibody

[Similar Products]
 Matching Pairs    Unconjugated Antibody: NKCC2 (MBS805560)
HRP Conjugated Antibody: NKCC2 (MBS805913)
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 3D Structure    ModBase 3D Structure for Q5PQV1
 Clonality    Polyclonal
 Host    Rabbit
 Species Reactivity    Human, Mouse, Rat
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 Form/Format    Affinity Purified
 Concentration    1mg/mL (lot specific)
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 Conjugate    HRP
 Immunogen    Produced against a synthetic peptide mapping to a segment (amino acids 33-55) of the N-terminal tail of rat NKCC2 (antibody desgination L224).
 Research Area(s)    Cancer, Neuroscience, Pumps/Transporters, Ion Pumps/Transporters
 Cellular Localization    Membrane
 Storage Buffer    PBS, 50% glycerol, 0.09% sodium azide
 Certifcate of Analysis    1ug/ml of MBS805913 was sufficient for detection of NKCC2 in 10ug of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
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 Preparation and Storage    20 degree C; 1 year+. Avoid freeze/ thaw cycle.
 Other Notes    Small volumes of anti-Slc12a1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-Slc12a1 antibody

   Background Info: Detects ~160kDa

Scientific Background: NKCC2, a kidney-specific member of the cation chloride co-transporter family SLC12, facilitates transport of sodium, potassium, and chloride ions from the lumen of the renal thick ascending limb into the cell (1, 2). Transport of sodium dilutes the luminal fluid, decreasing its osmolality creating an osmotic driving force for water reabsorption in the connecting tubule and cortical collecting duct under the influence of the hormone vasopressin. NKCC2 is found on the apical surface of the thick ascending limb of the loop of Henle and is blocked by loop diuretics such as furosemide (1).
 Product Categories/Family for anti-Slc12a1 antibody    Ion Channels, Transporters
 Applications Tested/Suitable for anti-Slc12a1 antibody   

Western Blot (WB), Immunohistochemistry (IHC)

 Application Notes for anti-Slc12a1 antibody    WB (1:1000); optimal dilutions for assays should be determined by the user.
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 Western Blot (WB) of anti-Slc12a1 antibody    Western blot analysis of Rat kidney tissue lysates showing detection of NKCC2 protein using Rabbit Anti-NKCC2 Polyclonal Antibody (SPC-401). Primary Antibody: Rabbit Anti-NKCC2 Polyclonal Antibody (SPC-401) at 1:1000.
anti-Slc12a1 antibody Western Blot (WB) (WB) image
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NCBI/Uniprot data below describe general gene information for Slc12a1. It may not necessarily be applicable to this product.
 NCBI GI #    162287159
 NCBI GeneID    25065
 NCBI Accession #    NP_062007 [Other Products]
 NCBI GenBank Nucleotide #    NM_019134.2 [Other Products]
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 UniProt Primary Accession #    Q5PQV1 [Other Products]
 UniProt Related Accession #    P55016 [Other Products]
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 NCBI Official Synonym Full Names    solute carrier family 12 (sodium/potassium/chloride transporter), member 1
 NCBI Official Symbol    Slc12a1 [Similar Products]
 NCBI Official Synonym Symbols   
BSC1; Nkcc2
[Similar Products]
 NCBI Protein Information    solute carrier family 12 member 1
 UniProt Protein Name    RCG26347, isoform CRA_c
 UniProt Synonym Protein Names   
RCG26347, isoform CRA_c; Slc12a1 protein; Solute carrier family 12 member 1
 Protein Family    Solute carrier family
 UniProt Gene Name    Slc12a1 [Similar Products]
 UniProt Synonym Gene Names    rCG_26347 [Similar Products]
 UniProt Entry Name    Q5PQV1_RAT
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 NCBI Summary for Slc12a1    has bumetamide-sensitive Na(+)-(K+)-Cl- symporter activity; may play a role in sodium and chloride transport in the kidney [RGD, Feb 2006]
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 UniProt Comments for Slc12a1    NKCC2: Electrically silent transporter system. Mediates sodium and chloride reabsorption. Plays a vital role in the regulation of ionic balance and cell volume. Kidney specific. Activated by WNK3. Belongs to the SLC12A transporter family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Transporter, SLC family; Membrane protein, integral; Transporter

Cellular Component: cell surface; apical plasma membrane; plasma membrane; integral to membrane

Molecular Function: sodium:potassium:chloride symporter activity; cation:chloride symporter activity

Biological Process: diterpenoid metabolic process; sodium ion transport; protein insertion into membrane from inner side; chloride transport; excretion; kidney development; potassium ion transport; chemical homeostasis
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Product References and Citations for anti-Slc12a1 antibody

   1.Caceres P.S., Ares G.R., and Ortiz P.A. (2009) J Biol Chem. 284(37): 24965-24971. 2.Xue H., et al. (2009) Neurogastroentrol Motil. 21(10): 1068-1089.
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 Research Articles on Slc12a1    1. Report tubuloglomerular and connecting tubuloglomerular feedback during inhibition of various Na transporters in the nephron.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with anti-Slc12a1 antibodyDiseases associated with anti-Slc12a1 antibody
 Products by Pathway  Pathway Diagram
 Cation-coupled Chloride Cotransporters Pathway antibodies  Cation-coupled Chloride Cotransporters Pathway Diagram
 SLC-mediated Transmembrane Transport Pathway antibodies  SLC-mediated Transmembrane Transport Pathway Diagram
 Transmembrane Transport Of Small Molecules Pathway antibodies  Transmembrane Transport Of Small Molecules Pathway Diagram
 Transport Of Inorganic Cations/anions And Amino Acids/oligopeptides Pathway antibodies  Transport Of Inorganic Cations/anions And Amino Acids/oligopeptides Pathway Diagram
 Disease Name  Pubmed Publications
 Kidney Diseases Antibodies  >73 publications with Slc12a1 and Kidney Diseases
 Hypertension Antibodies  >72 publications with Slc12a1 and Hypertension
 Bartter Syndrome Antibodies  >39 publications with Slc12a1 and Bartter Syndrome
 Disease Models, Animal Antibodies  >27 publications with Slc12a1 and Disease Models, Animal
 Hypokalemia Antibodies  >24 publications with Slc12a1 and Hypokalemia
 Polyuria Antibodies  >18 publications with Slc12a1 and Polyuria
 Nervous System Diseases Antibodies  >13 publications with Slc12a1 and Nervous System Diseases
 Renal Insufficiency Antibodies  >10 publications with Slc12a1 and Renal Insufficiency
 Hypotension Antibodies  >7 publications with Slc12a1 and Hypotension
 Heart Diseases Antibodies  >7 publications with Slc12a1 and Heart Diseases
Organs/Tissues associated with anti-Slc12a1 antibody
 Organ/Tissue Name  Pubmed Publications
 Kidney Antibodies  >225 publications with Slc12a1 and Kidney
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