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anti-NKX2-5 antibody :: Goat NKX2-5 Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS248997
Unit / Price
0.05 mg  /  $530 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

NKX2-5, Polyclonal Antibody

★Popular Item★
 Also Known As   

Goat Polyclonal to Human NKX2-5

 Product Synonym Names    Anti-NKX2-5 Antibody (Internal) IHC-plus; NKX2-5; Cardiac-specific homeo box; Cardiac-specific homeobox 1; Cardiac-specific homeobox; CSX1; CHNG5; HLHS2; NKX2E; NKX4-1; NKX2.5; Tinman paralog; VSD3; CSX; Homeobox protein CSX; Homeobox protein Nkx-2.5; NK2 homeobox 5; Human NKX2-5
 Product Gene Name   

anti-NKX2-5 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    phenotype 614435
 3D Structure    ModBase 3D Structure for P52952
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 Clonality    Polyclonal
 Host    Goat
 Species Reactivity    Reactivity: Human, Monkey (tested or 100% immunogen sequence identity)
Predicted: Rat, Hamster, Pig (at least 90% immunogen sequence identity)
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 Specificity    Human NKX2-5. This antibody is expected to recognize all three reported isoforms (NP_004378.1; NP_001159647.1; NP_001159648.1).
 Purity/Purification    Immunoaffinity Purified
 Form/Format    Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
 Concentration    0.5 mg/ml (lot specific)
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 Target Species    Human
 Immunogen Description    Synthetic peptide C-PRAYSDPDPAKDPR from an internal region of human NKX2-5 (NP_004378.1; NP_001159647.1; NP_001159648.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset (100%); Rat, Hamster, Elephant, Panda, Pig (93%).
 Immunogen Type    Synthetic peptide
 Immunogen    NKX2-5 antibody was raised against synthetic peptide C-PRAYSDPDPAKDPR from an internal region of human NKX2-5 (NP_004378.1; NP_001159647.1; NP_001159648.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset (100%); Rat, Hamster, Elephant, Panda, Pig (93%); Bovine, Dog (86%).
 Antigen Modification    Internal
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 Preparation and Storage    Store at -20 degree C. Minimize freezing and thawing.
 Other Notes    Small volumes of anti-NKX2-5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-NKX2-5 antibody

   Nkx 2.5 is a lineage restricted transcription factor that regulates the expression of heart specific genes. This regulation is essential for tissue differentiation and the determination of temporal and spatial patterns of development. Abundant expression of Nkx 2.5 is observed in the mouse heart from the time of cardiac differentiation through adult life. Targeted disruption of Nkx 2.5 in mice results in early embryonic lethality with cardiac development arrested at the linear tube stage, prior to looping.
 Applications Tested/Suitable for anti-NKX2-5 antibody   

Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)

 Application Notes for anti-NKX2-5 antibody    Usage Summary: Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 45kDa band observed in Human Heart lysates (calculated MW of 34.9kDa according to NP_004378.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Shiojima et al, Circ Res. 1996 Nov;79(5):920-9.; PMID: 8888684). Recommended concentration: 0.3-1 ug/ml.

Uses: IHC - Paraffin (3.75 g/ml), Western blot (0.3 - 1 g/ml), ELISA (1:32000)
Optimal dilution to be determined by the researcher.
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 Immunohistochemistry (IHC) of anti-NKX2-5 antibody    Anti-NKX2-5 antibody IHC staining of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
anti-NKX2-5 antibody Immunohistochemistry (IHC) (IHC) image
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 Western Blot (WB) of anti-NKX2-5 antibody    NKX2-5 antibody (0.3 ug/ml) staining of Human Heart lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
anti-NKX2-5 antibody Western Blot (WB) (WB) image
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NCBI/Uniprot data below describe general gene information for NKX2-5. It may not necessarily be applicable to this product.
 NCBI GI #    4758090
 NCBI GeneID    1482
 NCBI Accession #    NP_004378.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_004387.3 [Other Products]
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 UniProt Primary Accession #    P52952 [Other Products]
 UniProt Secondary Accession #    A8K3K0; B4DNB6; E9PBU6 [Other Products]
 UniProt Related Accession #    P52952 [Other Products]
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 NCBI Official Full Name    homeobox protein Nkx-2.5 isoform 1
 NCBI Official Synonym Full Names    NK2 homeobox 5
 NCBI Official Symbol    NKX2-5 [Similar Products]
 NCBI Official Synonym Symbols   
CSX; CSX1; VSD3; CHNG5; HLHS2; NKX2E; NKX2.5; NKX4-1
[Similar Products]
 NCBI Protein Information    homeobox protein Nkx-2.5; tinman paralog; homeobox protein CSX; cardiac-specific homeobox 1; homeobox protein NK-2 homolog E; NK2 transcription factor related, locus 5
 UniProt Protein Name    Homeobox protein Nkx-2.5
 UniProt Synonym Protein Names   
Cardiac-specific homeobox; Homeobox protein CSX; Homeobox protein NK-2 homolog E
 Protein Family    Homeobox protein
 UniProt Gene Name    NKX2-5 [Similar Products]
 UniProt Synonym Gene Names    CSX; NKX2.5; NKX2E [Similar Products]
 UniProt Entry Name    NKX25_HUMAN
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 NCBI Summary for NKX2-5    This gene encodes a homeobox-containing transcription factor. This transcription factor functions in heart formation and development. Mutations in this gene cause atrial septal defect with atrioventricular conduction defect, and also tetralogy of Fallot, which are both heart malformation diseases. Mutations in this gene can also cause congenital hypothyroidism non-goitrous type 5, a non-autoimmune condition. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]
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 UniProt Comments for NKX2-5    NKX2-5: Implicated in commitment to and/or differentiation of the myocardial lineage. Acts as a transcriptional activator of ANF in cooperation with GATA4. Interacts with HIPK1 and HIPK2, but not HIPK3. Interacts with the C-terminal zinc finger of GATA4 through its homeobox domain. Also interacts with JARID2 which represses its ability to activate transcription of ANF. Interacts with FBLIM1. Expressed only in the heart. Belongs to the NK-2 homeobox family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 5q34

Cellular Component: transcription factor complex; cytoplasm; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; protein homodimerization activity; DNA binding; sequence-specific DNA binding; protein heterodimerization activity; chromatin binding; transcription factor activity; transcription factor binding

Biological Process: transcription from RNA polymerase II promoter; heart morphogenesis; ventricular cardiac myofibril development; positive regulation of transcription, DNA-dependent; ventricular cardiac muscle cell development; cardiac muscle cell proliferation; negative regulation of transcription from RNA polymerase II promoter; Wnt receptor signaling pathway through beta-catenin; cardiac muscle cell differentiation; BMP signaling pathway; atrial cardiac muscle cell development; positive regulation of cell proliferation; cardiac muscle morphogensis; thyroid gland development; regulation of cardiac muscle cell proliferation; hemopoiesis; heart looping; vasculogenesis; cell differentiation; cardiac muscle contraction; spleen development; adult heart development; pharyngeal system development; sarcomere organization; positive regulation of cardioblast differentiation; embryonic heart tube development; positive regulation of transcription from RNA polymerase II promoter; positive regulation of neuron differentiation; negative regulation of transcription, DNA-dependent; positive regulation of heart contraction; negative regulation of apoptosis

Disease: Ventricular Septal Defect 3; Atrial Septal Defect 7 With Or Without Atrioventricular Conduction Defects; Conotruncal Heart Malformations; Tetralogy Of Fallot; Hypothyroidism, Congenital, Nongoitrous, 5; Hypoplastic Left Heart Syndrome 2
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 Research Articles on NKX2-5    1. Art27 interacts with GATA4, FOG2 and NKX2.5 and is a novel co-repressor of cardiac genes.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-NKX2-5 antibodyPathways associated with anti-NKX2-5 antibody
 Reference Product  PubMed Publications
 GATA4 antibody  >321 publications with NKX2-5 and GATA4
 TBX5 antibody  >119 publications with NKX2-5 and TBX5
 MEF2C antibody  >109 publications with NKX2-5 and MEF2C
 NPPA antibody  >16 publications with NKX2-5 and NPPA
 SMAD4 antibody  >9 publications with NKX2-5 and SMAD4
 MYOCD antibody  >8 publications with NKX2-5 and MYOCD
 ANKRD1 antibody  >7 publications with NKX2-5 and ANKRD1
 FOXH1 antibody  >7 publications with NKX2-5 and FOXH1
 SMAD2 antibody  >6 publications with NKX2-5 and SMAD2
 Products by Pathway  Pathway Diagram
 Cardiac Progenitor Differentiation Pathway antibodies  Cardiac Progenitor Differentiation Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 Generic Transcription Pathway antibodies  Generic Transcription Pathway Diagram
 Heart Development Pathway antibodies  Heart Development Pathway Diagram
 Regulation Of Nuclear SMAD2/3 Signaling Pathway antibodies  Regulation Of Nuclear SMAD2/3 Signaling Pathway Diagram
 SRF And MiRs In Smooth Muscle Differentiation And Proliferation Pathway antibodies  SRF And MiRs In Smooth Muscle Differentiation And Proliferation Pathway Diagram
 YAP1- And WWTR1 (TAZ)-stimulated Gene Expression Pathway antibodies  YAP1- And WWTR1 (TAZ)-stimulated Gene Expression Pathway Diagram
Diseases associated with anti-NKX2-5 antibodyOrgans/Tissues associated with anti-NKX2-5 antibody
 Disease Name  Pubmed Publications
 ATRIAL SEPTAL DEFECT 7 WITH OR WITHOUT ATRIOVENTRICULAR CONDUCTION DEFECTS Antibodies  >1624 publications with NKX2-5 and ATRIAL SEPTAL DEFECT 7 WITH OR WITHOUT ATRIOVENTRICULAR CONDUCTION DEFECTS
 Heart Diseases Antibodies  >351 publications with NKX2-5 and Heart Diseases
 Cardiovascular Diseases Antibodies  >345 publications with NKX2-5 and Cardiovascular Diseases
 Heart Defects, Congenital Antibodies  >232 publications with NKX2-5 and Heart Defects, Congenital
 Myocardial Infarction Antibodies  >75 publications with NKX2-5 and Myocardial Infarction
 Hypertrophy Antibodies  >47 publications with NKX2-5 and Hypertrophy
 Disease Models, Animal Antibodies  >37 publications with NKX2-5 and Disease Models, Animal
 Tetralogy of Fallot Antibodies  >31 publications with NKX2-5 and Tetralogy of Fallot
 Cardiomyopathies Antibodies  >26 publications with NKX2-5 and Cardiomyopathies
 Fibrosis Antibodies  >15 publications with NKX2-5 and Fibrosis
 Organ/Tissue Name  Pubmed Publications
 Heart Antibodies  >973 publications with NKX2-5 and Heart
 Embryonic Tissue Antibodies  >393 publications with NKX2-5 and Embryonic Tissue
 Lung Antibodies  >21 publications with NKX2-5 and Lung
 Brain Antibodies  >19 publications with NKX2-5 and Brain
 Liver Antibodies  >16 publications with NKX2-5 and Liver
 Spleen Antibodies  >15 publications with NKX2-5 and Spleen
 Intestine Antibodies  >7 publications with NKX2-5 and Intestine
 Uncharacterized Tissue Antibodies  >4 publications with NKX2-5 and Uncharacterized Tissue
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