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anti-GRIN1 antibody :: Rabbit anti-Mouse, rat NMDA NR1 Subunit, Splice Variant C1 Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS502079
Testing Data
Unit / Price
0.025 mg  /  $360 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

NMDA NR1 Subunit, Splice Variant C1 (GRIN1), Polyclonal Antibody

★Popular Item★
 Also Known As   

Anti-NMDA Receptor, NR1 Subunit, C1 Splice Variant Insert

 Product Gene Name   

anti-GRIN1 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Chromosome Location    Chromosome: 3; NC_005102.3 (2489158..2516082, complement). Location: 3p13
 3D Structure    ModBase 3D Structure for P35439
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 Clonality    Polyclonal
 Host    Rabbit
 Species Reactivity    Mouse, rat
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 Specificity    Specific for the ~120k NR1 subunit of the NMDA receptor containing the C1 splice variant insert. Does not recognize the NR1 subunits of the NMDA receptor that do not contain the C1 insert.
 Purity/Purification    Affinity Purified (Prepared from rabbit serum by affinity purification using a column to which the peptide immunogen was coupled.)
 Form/Format    25 ug antibody vial; lyophilized from 5 mM ammonium bicarbonate. The antibody should be reconstituted in 50 ul phosphate buffered saline (PBS: 137 mM NaCl, 7.5 mM Na2HPO4, 2.7 mM KCl, 1.5 mM KH2PO4, pH 7.4) before use. After reconstitution the antibody sh
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 Antigen    Peptide from the NR1 subunit, C1 splice variant insert of the NMDA receptor.
 Immunogen Information    Synthetic peptide corresponding to amino acid residues specific to the NR1 subunit, C1 splice variant insert conjugated to KLH
 Immunogen Species    Rat
 Reactivity Assumed Based on 100% Sequence Homology    Bovine, canine, human, non-human primates
 Species Reactivity Note    The antibody has been directly tested for reactivity in Western blots in rat and mouse tissues. It is anticipated that the antibody will also work with bovine, canine, human, and non-human primate tissues based on the fact that these species have 100% homology with the amino acid sequence used as antigen.
 Biological Significance    The ion channels activated by glutamate that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA Receptors (NMDAR). The NMDAR plays an essential role in memory and neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer's, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002; Wenthold et al., 2003; Carroll and Zukin, 2002). There are a number of different splice variants of the NR1-Subunit (Foldes et al., 1994; Zukin and Bennett, 1995). Differential splicing of three exons in the NR1-Subunit generates up to eight NR1-Subunit splice variants and 7 of these have been identified in cDNA libraries. These exons encode a 21 amino acid N-terminal domain (N1) and adjacent sequences in the C-terminus (C1 and C2). Splicing out the C2 cassette eliminates the first stop codon and produces a new reading frame that generates a new sequence of 22 amino acids (C2'). Considerable attention has been focused on the distribution and expression of these splice variants that may affect the functional properties and regulation of the NMDAR.
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 Preparation and Storage    For long term storage -20 degree C is recommended. Stable at -20 degree C for at least 1 year.
 Other Notes    Small volumes of anti-GRIN1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-GRIN1 antibody

   Affinity purified rabbit polyclonal antibody
 Applications Tested/Suitable for anti-GRIN1 antibody   

Western Blot (WB), Immunohistochemistry (IHC)

 Application Notes for anti-GRIN1 antibody    Quality Control: Western blots performed on each lot.
WB: 1:1000
IHC (frozen sections; unpublished observations): 1:1000 to 1:2000
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 Testing Data of anti-GRIN1 antibody    anti-GRIN1 antibody Testing Data image
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NCBI/Uniprot data below describe general gene information for GRIN1. It may not necessarily be applicable to this product.
 NCBI GI #    396578144
 NCBI GeneID    24408
 NCBI Accession #    NP_001257531.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001270602.1 [Other Products]
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 UniProt Primary Accession #    P35439 [Other Products]
 UniProt Secondary Accession #    Q62646 [Other Products]
 UniProt Related Accession #    P35439 [Other Products]
 Molecular Weight    120
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 NCBI Official Full Name    glutamate receptor ionotropic, NMDA 1 isoform 1b
 NCBI Official Synonym Full Names    glutamate receptor, ionotropic, N-methyl D-aspartate 1
 NCBI Official Symbol    Grin1 [Similar Products]
 NCBI Official Synonym Symbols   
NR1; GluN1; NMDAR1
[Similar Products]
 NCBI Protein Information    glutamate receptor ionotropic, NMDA 1; NMD-R1; neurotransmitter receptor; NMDA R1 receptor C1 cassette; N-methyl-D-aspartate glutamate receptor; glutamate [NMDA] receptor subunit zeta-1; N-methyl-D-aspartate receptor subunit NR1
 UniProt Protein Name    Glutamate receptor ionotropic, NMDA 1
 UniProt Synonym Protein Names   
Glutamate [NMDA] receptor subunit zeta-1; N-methyl-D-aspartate receptor subunit NR1
 Protein Family    Glutamate receptor ionotropic
 UniProt Gene Name    Grin1 [Similar Products]
 UniProt Synonym Gene Names    Nmdar1; GluN1; NMD-R1 [Similar Products]
 UniProt Entry Name    NMDZ1_RAT
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 NCBI Summary for GRIN1    subunit of NMDA-preferring ionotropic glutamate receptors; may play a role in long term potentiation [RGD, Feb 2006]
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 UniProt Comments for GRIN1    Function: NMDA receptor subtype of glutamate-gated ion channels possesses high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. Plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. It mediates neuronal functions in glutamate neurotransmission. Is involved in the cell surface targeting of NMDA receptors. Ref.18

Subunit structure: Forms heteromeric channel of a zeta subunit (GRIN1), a epsilon subunit (GRIN2A, GRIN2B, GRIN2C or GRIN2D) and a third subunit (GRIN3A or GRIN3B); disulfide-linked

By similarity. Found in a complex with GRIN2A or GRIN2B, GRIN3A and PPP2CB. Found in a complex with GRIN2A or GRIN2B and GRIN3B. Interacts with SNX27 (via PDZ domain); the interaction is required for recycling to the plasma membrane when endocytosed and prevent degradation in lysosomes

By similarity. Interacts with DLG4 and MPDZ. Interacts with LRFN1 and LRFN2. Interacts with MYZAP. Ref.7 Ref.8 Ref.9 Ref.10 Ref.11 Ref.13 Ref.14 Ref.15 Ref.17

Subcellular location: Cell membrane; Multi-pass membrane protein

By similarity. Cell junction › synapse › postsynaptic cell membrane

By similarity. Cell junction › synapse › postsynaptic cell membrane › postsynaptic density

By similarity. Note: Enriched in postsynaptic plasma membrane and postsynaptic densities

By similarity.

Post-translational modification: NMDA is probably regulated by C-terminal phosphorylation of an isoform ofNR1 by PKC. Dephosphorylated on Ser-897 probably by protein phosphatase 2A (PPP2CB). Its phosphorylated state is influenced by the formation of the NMDAR-PPP2CB complex and the NMDAR channel activity. Ref.7 Ref.12

Sequence similarities: Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR1/GRIN1 subfamily. [View classification]
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Product References and Citations for anti-GRIN1 antibody

   • Carroll RC, Zukin RS (2002) NMDA-receptor trafficking and targeting: implications for synaptic transmission and plasticity. Trends Neurosci 25:571-577.
• Foldes RL, Rampersad V, Kamboj RK (1994) Cloning and sequence analysis of additional splice variants encoding human N-methyl-D-aspartate receptor (hNR1) subunits. Gene 147:303-304.
• Grosshans DR, Clayton DA, Coultrap SJ, Browning MD (2002) LTP leads to rapid surface expression of NMDA but not AMPA receptors in adult rat CA1. Nat Neurosci 5:27-33.
• Wenthold RJ, Prybylowski K, Standley S, Sans N, Petralia RS (2003) Trafficking of NMDA receptors. Annu Rev Pharmacol Toxicol 43:335-358
• Zukin RS, Bennett MVL (1995) Alternatively spliced isoforms of the NMDARI receptor subunit. Trends Neurosci 18:306-313.
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 Research Articles on GRIN1    1. These results suggest that PI3K/Akt and NMDAR independently regulate spinal plasticity in visceral pain model
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with anti-GRIN1 antibodyDiseases associated with anti-GRIN1 antibody
 Products by Pathway  Pathway Diagram
 Activation Of NMDA Receptor Upon Glutamate Binding And Postsynaptic Events Pathway antibodies  Activation Of NMDA Receptor Upon Glutamate Binding And Postsynaptic Events Pathway Diagram
 Alcoholism Pathway antibodies  Alcoholism Pathway Diagram
 Alcoholism Pathway antibodies  Alcoholism Pathway Diagram
 Alzheimer's Disease Pathway antibodies  Alzheimer's Disease Pathway Diagram
 Alzheimer's Disease Pathway antibodies  Alzheimer's Disease Pathway Diagram
 Amphetamine Addiction Pathway antibodies  Amphetamine Addiction Pathway Diagram
 Amphetamine Addiction Pathway antibodies  Amphetamine Addiction Pathway Diagram
 Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies  Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
 Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies  Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
 CREB Phosphorylation Through The Activation Of CaMKII Pathway antibodies  CREB Phosphorylation Through The Activation Of CaMKII Pathway Diagram
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >22 publications with GRIN1 and Nervous System Diseases
 Brain Diseases Antibodies  >12 publications with GRIN1 and Brain Diseases
 Disease Models, Animal Antibodies  >7 publications with GRIN1 and Disease Models, Animal
 Memory Disorders Antibodies  >5 publications with GRIN1 and Memory Disorders
 Nerve Degeneration Antibodies  >5 publications with GRIN1 and Nerve Degeneration
 Movement Disorders Antibodies  >4 publications with GRIN1 and Movement Disorders
 Learning Disorders Antibodies  >4 publications with GRIN1 and Learning Disorders
 Neoplasms Antibodies  >4 publications with GRIN1 and Neoplasms
 Pain Antibodies  >4 publications with GRIN1 and Pain
 Seizures Antibodies  >3 publications with GRIN1 and Seizures
Organs/Tissues associated with anti-GRIN1 antibody
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >58 publications with GRIN1 and Brain
 Eye Antibodies  >2 publications with GRIN1 and Eye
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