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anti-NOS3 antibody :: Rabbit NOS3 Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS175397
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 Product Name   

NOS3, Polyclonal Antibody

 Also Known As   

Polyclonal Anti-NOS3

 Product Synonym Names    Nitric oxide synthase; endothelial; nitric oxide synthase 3 (endothelial cell); Constitutive NOS|
 Product Gene Name   

anti-NOS3 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Chromosome Location    Chromosome: 7; NC_000007.13 (150688144..150711687). Location: 7q36
 OMIM    163729
 3D Structure    ModBase 3D Structure for P29474
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 Clonality    Polyclonal
 Isotype    Rabbit IgG
 Host    Rabbit
 Species Reactivity    Human, mouse, rat
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 Specificity    Human, Mouse, Rat. No cross reactivity with other proteins.
 Purity/Purification    Immunogen affinity purified.
 Form/Format    Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
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 Immunogen    A synthetic peptide corresponding to a sequence at the C-terminal of human NOS3(1138-1158 ELDEAGDVIGVLRDQQRYHED), identical to the related rat and mouse sequences.
 Reconstitution    Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
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 Preparation and Storage    At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time.Avoid repeated freezing and thawing.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Other Notes    Small volumes of anti-NOS3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-NOS3 antibody

   Rabbit IgG polyclonal antibody for Nitric oxide synthase, endothelial (NOS3) detection. Tested with WB,IHC-P in Human;Mouse;Rat.

NOS3 (Nitric Oxide Synthase 3), also called ENOS, a nitric oxide synthase that generates NO in blood vessels and is involved with regulating vascular tone by inhibiting smooth muscle contraction andplatelet aggregation. The NOS3 gene is mapped on 7q36.1. Variations in this gene are associated with susceptibility to coronary spasm. Fulton et al. (1999) concluded the eNOS is an AKT substrate linking signal transduction by AKT to the release of the gaseous second messenger nitric oxide. AKT mediates the activation of eNOS, leading to increased nitric oxide production. Inhibition of the PI3K AKT pathway or mutation of the AKT site on eNOS protein at serine-1177 attenuated the serine phosphorylation and prevented the activation of eNOS. RT-PCR analysis showed that expression of NOS3 in human umbilical vein endothelial cells (HUVECs) and human aortic vascular smooth muscle cells (HAOVSMCs) was inversely proportional to that of NOS3AS.
 Applications Tested/Suitable for anti-NOS3 antibody   

Western Blot (WB), Immunohistochemistry (IHC) Paraffin

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 Testing Data of anti-NOS3 antibody    anti-NOS3 antibody Testing Data image
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 Western Blot (WB) / Blotting #2 of anti-NOS3 antibody    anti-NOS3 antibody Western Blot (WB) / Blotting #2 (WB) image
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NCBI/Uniprot data below describe general gene information for NOS3. It may not necessarily be applicable to this product.
 NCBI GI #    40254422
 NCBI GeneID    4846
 NCBI Accession #    NP_000594.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_000603.4 [Other Products]
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 UniProt Primary Accession #    P29474 [Other Products]
 UniProt Secondary Accession #    Q13662; Q14251; Q14434; Q548C1; Q6GSL5; Q9UDC6; A0S0A7; A0S0A8; A8KA63; B2RCQ1; E9PFR2 [Other Products]
 UniProt Related Accession #    P29474 [Other Products]
 Molecular Weight    133,289 Da
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 NCBI Official Full Name    nitric oxide synthase, endothelial isoform 1
 NCBI Official Synonym Full Names    nitric oxide synthase 3 (endothelial cell)
 NCBI Official Symbol    NOS3 [Similar Products]
 NCBI Official Synonym Symbols   
[Similar Products]
 NCBI Protein Information    nitric oxide synthase, endothelial; cNOS; EC-NOS; NOSIII; NOS type III; endothelial NOS; constitutive NOS
 UniProt Protein Name    Nitric oxide synthase, endothelial
 UniProt Synonym Protein Names   
Constitutive NOS; cNOS; EC-NOS; Endothelial NOS; eNOS; NOS type III
 Protein Family    Nitric oxide synthase
 UniProt Gene Name    NOS3 [Similar Products]
 UniProt Synonym Gene Names    cNOS; eNOS; NOSIII [Similar Products]
 UniProt Entry Name    NOS3_HUMAN
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 NCBI Summary for NOS3    Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009]
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 UniProt Comments for NOS3    eNOS: endothelial constitutive nitric oxide synthase. Synthesizes nitric oxide (NO) from arginine and oxygen, which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. Induced in humans by caloric restriction. Stimulated by calcium/calmodulin. Inhibited by NOSIP and NOSTRIN.

Protein type: Amino Acid Metabolism - arginine and proline; EC; Oxidoreductase

Chromosomal Location of Human Ortholog: 7q36

Cellular Component: Golgi membrane; cytoskeleton; cytoplasm; plasma membrane; caveola; cytosol; nucleus

Molecular Function: actin monomer binding; calmodulin binding; protein binding; FAD binding; FMN binding; NADPH-hemoprotein reductase activity; nitric-oxide synthase activity; iron ion binding; heme binding; NADP binding; cadmium ion binding

Biological Process: regulation of systemic arterial blood pressure by endothelin; negative regulation of potassium ion transport; negative regulation of muscle hyperplasia; positive regulation of vasodilation; nitric oxide metabolic process; negative regulation of cell proliferation; regulation of the force of heart contraction by chemical signal; regulation of blood pressure; negative regulation of hydrolase activity; lipopolysaccharide-mediated signaling pathway; negative regulation of blood pressure; angiogenesis; nitric oxide biosynthetic process; regulation of blood vessel size; mitochondrion organization and biogenesis; interaction with host; negative regulation of calcium ion transport; in utero embryonic development; positive regulation of guanylate cyclase activity; regulation of sodium ion transport; ovulation from ovarian follicle; positive regulation of angiogenesis; response to heat; arginine catabolic process; endothelial cell migration; blood vessel remodeling; regulation of nitric-oxide synthase activity; vascular endothelial growth factor receptor signaling pathway; blood coagulation; nitric oxide mediated signal transduction; lung development; smooth muscle hyperplasia

Disease: Preeclampsia/eclampsia 1; Alzheimer Disease; Hypertension, Essential; Stroke, Ischemic
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Product References and Citations for anti-NOS3 antibody

   1.Ahsan, A., Norboo, T., Baig, M. A., Pasha, M. A. Q. Simultaneous selection of the wild-type genotypes of the G894T and 4B/4A polymorphisms of NOS3 associate with high-altitude adaptation. Ann. Hum. Genet. 69: 260-267, 2005.
2.Chen, C.-A., Wang, T.-Y., Varadharaj, S., Reyes, L. A., Hemann, C., Talukder, M. A. H., Chen, Y.-R., Druhan, L. J., Zweier, J. L. S-glutathionylation uncouples eNOS and regulates its cellular and vascular function. Nature 468: 1115-1118, 2010.
3.Fulton, D., Gratton, J.-P., McCabe, T. J., Fontana, J., Fujio, Y., Walsh, K., Franke, T. F., Papapetropoulos, A., Sessa, W. C. Regulation of endothelium-derived nitric oxide production by the protein kinase Akt. Nature 399: 597-601, 1999.
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 Research Articles on NOS3    1. This study suggests that the NOS3 T-786C polymorphism modifies the effect of leisure-time physical activity on serum triglyceride levels.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with anti-NOS3 antibodyDiseases associated with anti-NOS3 antibody
 Products by Pathway  Pathway Diagram
 ACE Inhibitor Pathway antibodies  ACE Inhibitor Pathway Diagram
 AGE/RAGE Pathway antibodies  AGE/RAGE Pathway Diagram
 Angiogenesis Pathway antibodies  Angiogenesis Pathway Diagram
 Angiopoietin Receptor Tie2-mediated Signaling Pathway antibodies  Angiopoietin Receptor Tie2-mediated Signaling Pathway Diagram
 Arginine And Proline Metabolism Pathway antibodies  Arginine And Proline Metabolism Pathway Diagram
 Arginine And Proline Metabolism Pathway antibodies  Arginine And Proline Metabolism Pathway Diagram
 Calcium Signaling Pathway antibodies  Calcium Signaling Pathway Diagram
 Calcium Signaling Pathway antibodies  Calcium Signaling Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Endothelin Pathway antibodies  Endothelin Pathway Diagram
 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >1904 publications with NOS3 and Cardiovascular Diseases
 Hypertension Antibodies  >940 publications with NOS3 and Hypertension
 Myocardial Ischemia Antibodies  >514 publications with NOS3 and Myocardial Ischemia
 Ischemia Antibodies  >491 publications with NOS3 and Ischemia
 Atherosclerosis Antibodies  >423 publications with NOS3 and Atherosclerosis
 Coronary Artery Disease Antibodies  >346 publications with NOS3 and Coronary Artery Disease
 Arteriosclerosis Antibodies  >321 publications with NOS3 and Arteriosclerosis
 Reperfusion Injury Antibodies  >247 publications with NOS3 and Reperfusion Injury
 Myocardial Infarction Antibodies  >222 publications with NOS3 and Myocardial Infarction
 Neoplasms Antibodies  >196 publications with NOS3 and Neoplasms
Organs/Tissues associated with anti-NOS3 antibody
 Organ/Tissue Name  Pubmed Publications
 Vascular Antibodies  >3278 publications with NOS3 and Vascular
 Blood Antibodies  >2459 publications with NOS3 and Blood
 Heart Antibodies  >1132 publications with NOS3 and Heart
 Kidney Antibodies  >479 publications with NOS3 and Kidney
 Brain Antibodies  >405 publications with NOS3 and Brain
 Lung Antibodies  >347 publications with NOS3 and Lung
 Liver Antibodies  >241 publications with NOS3 and Liver
 Embryonic Tissue Antibodies  >199 publications with NOS3 and Embryonic Tissue
 Bone Antibodies  >129 publications with NOS3 and Bone
 Skin Antibodies  >90 publications with NOS3 and Skin
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