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anti-ENO2 antibody :: Rabbit anti-Human, rat NSE (Neuron specific enolase) Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS502094
Testing Data
Unit / Price
0.1 mL  /  $315 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

NSE (Neuron specific enolase) (ENO2), Polyclonal Antibody

★Popular Item★
 Also Known As   

Anti-Neuron Specific Enolase (NSE)

 Product Gene Name   

anti-ENO2 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Chromosome Location    Chromosome: 12; NC_000012.12 (6914450..6923696). Location: 12p13
 OMIM    131360
 3D Structure    ModBase 3D Structure for P09104
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 Clonality    Polyclonal
 Host    Rabbit
 Species Reactivity    Human, rat
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 Specificity    Specific for the ~47kDa NSE protein.
 Purity/Purification    Neat Serum (Unpurified neat serum.)
 Form/Format    Liquid.
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 Antigen    Recombinant human NSE expressed in and purified from E. coli.
 Immunogen Information    Recombinant human NSE expressed in and purified from E. coli
 Immunogen Species    Human
 Species Reactivity Note    The antibody has been directly tested for reactivity in human and rat. It is expected that it will work on other mammalian tissues.
 Biological Significance    Neuron specific enolase (NSE) is an enzyme which catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate in the glycolytic pathway, and also the reverse reaction in gluconeogenesis. It is one of three mammalian enolases, which are also known as ENO1, ENO2, and ENO3 or alternately as enolase alpha, beta and gamma. The three enolases have different cell type specific expression patterns, so that antibodies to them are useful cell type specific markers.(MacAlesse et al., 1988). NSE corresponds to ENO2 or enolase gamma and is heavily expressed in neuronal cells. ENO1 is also known as enolase alpha and as non-neuronal enolase. The third enolase, ENO3 or enolase beta, is expressed in muscle cells. Since neurons require a great deal of energy, they are very rich in glycolytic enzymes such a GAPDH and NSE. Antibodies to this protein are therefore useful to identify neuronal cell bodies, developing neuronal lineage and neuroendocrine cells. Release of NSE from damaged neurons into CSF and blood has also been used as a biomarker of neuronal injury (2).
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 Preparation and Storage    Store at -20 degree C in undiluted aliquots; stable for at least 1 year. Avoid freeze/thaw cycles.
 Other Notes    Small volumes of anti-ENO2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-ENO2 antibody

   Rabbit polyclonal antibody
 Applications Tested/Suitable for anti-ENO2 antibody   

Western Blot (WB), Immunofluorescence (IF)

 Application Notes for anti-ENO2 antibody    Quality Control: Western blots performed on each lot.
WB: 1:2,000
IF: 1:500
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 Testing Data of anti-ENO2 antibody    anti-ENO2 antibody Testing Data image
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NCBI/Uniprot data below describe general gene information for ENO2. It may not necessarily be applicable to this product.
 NCBI GI #    5803011
 NCBI GeneID    2026
 NCBI Accession #    NP_001966.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001975.2 [Other Products]
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 UniProt Primary Accession #    P09104 [Other Products]
 UniProt Secondary Accession #    Q96J33 [Other Products]
 UniProt Related Accession #    P09104 [Other Products]
 Molecular Weight    47
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 NCBI Official Full Name    gamma-enolase
 NCBI Official Synonym Full Names    enolase 2 (gamma, neuronal)
 NCBI Official Symbol    ENO2 [Similar Products]
 NCBI Official Synonym Symbols   
NSE; HEL-S-279
[Similar Products]
 NCBI Protein Information    gamma-enolase; neural enolase; neuron-specific enolase; neurone-specific enolase; neuron specific gamma enolase; 2-phospho-D-glycerate hydrolyase; 2-phospho-D-glycerate hydro-lyase; epididymis secretory protein Li 279
 UniProt Protein Name    Gamma-enolase
 UniProt Synonym Protein Names   
2-phospho-D-glycerate hydro-lyase; Enolase 2; Neural enolase; Neuron-specific enolase
 Protein Family    Enolase
 UniProt Gene Name    ENO2 [Similar Products]
 UniProt Synonym Gene Names    NSE [Similar Products]
 UniProt Entry Name    ENOG_HUMAN
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 NCBI Summary for ENO2    This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates. [provided by RefSeq, Jul 2008]
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 UniProt Comments for ENO2    Function: Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival

By similarity.

Catalytic activity: 2-phospho-D-glycerate = phosphoenolpyruvate + H2O.

Cofactor: Magnesium. Required for catalysis and for stabilizing the dimer.

Pathway: Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.

Subunit structure: Mammalian enolase is composed of 3 isozyme subunits, alpha, beta and gamma, which can form homodimers or heterodimers which are cell-type and development-specific.

Subcellular location: Cytoplasm

By similarity. Cell membrane

By similarity. Note: Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form

By similarity.

Tissue specificity: The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.

Developmental stage: During ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells.

Induction: Levels of ENO2 increase dramatically in cardiovascular accidents, cerebral trauma, brain tumors and Creutzfeldt-Jakob disease.

Sequence similarities: Belongs to the enolase family.

Sequence caution: The sequence AAA52388.1 differs from that shown. Reason: Erroneous initiation.
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Product References and Citations for anti-ENO2 antibody

   • MacAlesse SM, Dunbar B, Fotherqill JE, Hinks LJ, Day IN (1988) Complete amino acid sequence of neurone-specific gamma isozyme of enolase (NSE) from human brain and comparison with the non-neuronal alpha form (NNE). Eur J Biochem. 178(2):413-17.
• Begaz, T., Kyriacou, D. N., Segal, J. and Bazarian, J. J. Serum biochemical markers for post-concussion syndrome in patients with mild traumatic brain injury. J. Neurotrauma 23:1201-1210 (2006).
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 Research Articles on ENO2    1. peripheral neuronal specific enolase may be a useful marker drug-naive major depression disorder and bipolar disorder, but its pathophysiological significance and response to treatment should be further investigated
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-ENO2 antibodyPathways associated with anti-ENO2 antibody
 Reference Product  PubMed Publications
 ALDOC antibody  >3 publications with ENO2 and ALDOC
 PCK1 antibody  >3 publications with ENO2 and PCK1
 GPI antibody  >2 publications with ENO2 and GPI
 PKM2 antibody  >2 publications with ENO2 and PKM2
 ALDOA antibody  >1 publications with ENO2 and ALDOA
 PKLR antibody  >1 publications with ENO2 and PKLR
 Products by Pathway  Pathway Diagram
 Biosynthesis Of Amino Acids Pathway antibodies  Biosynthesis Of Amino Acids Pathway Diagram
 Biosynthesis Of Amino Acids Pathway antibodies  Biosynthesis Of Amino Acids Pathway Diagram
 Carbon Metabolism Pathway antibodies  Carbon Metabolism Pathway Diagram
 Carbon Metabolism Pathway antibodies  Carbon Metabolism Pathway Diagram
 Gluconeogenesis Pathway antibodies  Gluconeogenesis Pathway Diagram
 Gluconeogenesis, Oxaloacetate => Fructose-6P Pathway antibodies  Gluconeogenesis, Oxaloacetate => Fructose-6P Pathway Diagram
 Gluconeogenesis, Oxaloacetate => Fructose-6P Pathway antibodies  Gluconeogenesis, Oxaloacetate => Fructose-6P Pathway Diagram
 Glucose Metabolism Pathway antibodies  Glucose Metabolism Pathway Diagram
 Glycolysis Pathway antibodies  Glycolysis Pathway Diagram
 Glycolysis (Embden-Meyerhof Pathway), Glucose => Pyruvate antibodies  Glycolysis (Embden-Meyerhof Pathway), Glucose => Pyruvate Diagram
Diseases associated with anti-ENO2 antibodyOrgans/Tissues associated with anti-ENO2 antibody
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >12 publications with ENO2 and Neoplasms
 Disease Models, Animal Antibodies  >7 publications with ENO2 and Disease Models, Animal
 Nervous System Diseases Antibodies  >5 publications with ENO2 and Nervous System Diseases
 Brain Diseases Antibodies  >4 publications with ENO2 and Brain Diseases
 Adenocarcinoma Antibodies  >3 publications with ENO2 and Adenocarcinoma
 Inflammation Antibodies  >2 publications with ENO2 and Inflammation
 Kidney Diseases Antibodies  >2 publications with ENO2 and Kidney Diseases
 Uterine Cervical Neoplasms Antibodies  >2 publications with ENO2 and Uterine Cervical Neoplasms
 Hypertension Antibodies  >2 publications with ENO2 and Hypertension
 Prostatic Neoplasms Antibodies  >2 publications with ENO2 and Prostatic Neoplasms
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >16 publications with ENO2 and Brain
 Muscle Antibodies  >8 publications with ENO2 and Muscle
 Blood Antibodies  >8 publications with ENO2 and Blood
 Skin Antibodies  >4 publications with ENO2 and Skin
 Intestine Antibodies  >4 publications with ENO2 and Intestine
 Lung Antibodies  >4 publications with ENO2 and Lung
 Kidney Antibodies  >4 publications with ENO2 and Kidney
 Bone Antibodies  >3 publications with ENO2 and Bone
 Liver Antibodies  >3 publications with ENO2 and Liver
 Embryonic Tissue Antibodies  >3 publications with ENO2 and Embryonic Tissue
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