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anti-NT5E antibody :: Rabbit anti-Human, Mouse NT5E / eNT / CD73 Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS242461
Unit / Price
0.2 mL  /  $530 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

NT5E / eNT / CD73, Polyclonal Antibody

★Popular Item★
 Also Known As   

Rabbit Polyclonal to Human NT5E / eNT / CD73

 Product Synonym Names    Anti-NT5E / eNT / CD73 Antibody (C-Terminus) IHC-plus; NT5E; 5-nucleotidase; ecto (CD73); 5'-nucleotidase; 5 nucleotidase (CD73); 5-NT; 5-nucleotidase; 5'-nucleotidase; ecto (CD73); CD73; CD73 antigen; E5NT; Ecto-5-nucleotidase; ENT; EN; NT; Purine 5-Prime-Nucleotidase; NT5; NTE; Human NT5E; eNT; CD73
 Product Gene Name   

anti-NT5E antibody

[Similar Products]
 Product Synonym Gene Name    eNT; CD73 [Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    gene 211800
 3D Structure    ModBase 3D Structure for P21589
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 Clonality    Polyclonal
 Host    Rabbit
 Species Reactivity    Human, Mouse
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 Specificity    Cross-reactivity with mouse has been confirmed in WB only. The antibody has not been evaluated for use in IHC with mouse tissues at time.
 Purity/Purification    Ammonium sulfate precipitation
 Form/Format    PBS, 0.09% sodium azide
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 Target Species    Human
 Immunogen Description    KLH conjugated synthetic peptide selected from the C-terminal region of human NT5E.
 Immunogen Type    Synthetic peptide
 Immunogen    NT5E / eNT / CD73 antibody was raised against kLH conjugated synthetic peptide selected from the C-terminal region of human NT5E.
 Antigen Modification    C-Terminus
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 Preparation and Storage    Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.
 Other Notes    Small volumes of anti-NT5E antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Applications Tested/Suitable for anti-NT5E antibody   

Immunohistochemistry (IHC - Paraffin), Western Blot (WB)

 Application Notes for anti-NT5E antibody    IHC-P (10 ug/ml), WB (1:1000)
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
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 Immunohistochemistry (IHC) of anti-NT5E antibody    Anti-NT5E / CD73 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
anti-NT5E antibody Immunohistochemistry (IHC) (IHC) image
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 Immunohistochemistry (IHC) of anti-NT5E antibody    Formalin-fixed and paraffin-embedded human Placenta tissue reacted with CD73 (NT5E) Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
anti-NT5E antibody Immunohistochemistry (IHC) (IHC) image
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 Western Blot (WB) of anti-NT5E antibody    Western blot of mouse brain tissue lysate.
anti-NT5E antibody Western Blot (WB) (WB) image
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 Western Blot (WB) of anti-NT5E antibody    Western blot analysis of CD73 Pab in mouse brain tissue lysate. CD73 (Arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
anti-NT5E antibody Western Blot (WB) (WB) image
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 Western Blot (WB) of anti-NT5E antibody    Western blot analysis of NT5E (arrow) using rabbit polyclonal NT5E Antibody (M535). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the NT5E gene.
anti-NT5E antibody Western Blot (WB) (WB) image
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NCBI/Uniprot data below describe general gene information for NT5E. It may not necessarily be applicable to this product.
 NCBI GI #    4505467
 NCBI GeneID    4907
 NCBI Accession #    NP_002517.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_002526.3 [Other Products]
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 UniProt Primary Accession #    P21589 [Other Products]
 UniProt Secondary Accession #    O75520; Q5W116; B3KQI8 [Other Products]
 UniProt Related Accession #    P21589 [Other Products]
 Molecular Weight    57,949 Da
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 NCBI Official Full Name    5'-nucleotidase isoform 1 preproprotein
 NCBI Official Synonym Full Names    5'-nucleotidase, ecto (CD73)
 NCBI Official Symbol    NT5E [Similar Products]
 NCBI Official Synonym Symbols   
NT; eN; NT5; NTE; eNT; CD73; E5NT; CALJA
[Similar Products]
 NCBI Protein Information    5'-nucleotidase; 5'-NT; ecto-5'-nucleotidase; Purine 5-Prime-Nucleotidase
 UniProt Protein Name    5'-nucleotidase
 UniProt Synonym Protein Names   
Ecto-5'-nucleotidase; CD_antigen: CD73
 UniProt Gene Name    NT5E [Similar Products]
 UniProt Synonym Gene Names    NT5; NTE; 5'-NT [Similar Products]
 UniProt Entry Name    5NTD_HUMAN
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 NCBI Summary for NT5E    The protein encoded by this gene is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2011]
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 UniProt Comments for NT5E    NT5E: Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Homodimer; disulfide-linked. Belongs to the 5'-nucleotidase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, GPI anchor; Phosphatase (non-protein); Cofactor and Vitamin Metabolism - nicotinate and nicotinamide; Nucleotide Metabolism - purine; Nucleotide Metabolism - pyrimidine; EC 3.1.3.5; Membrane protein, integral

Chromosomal Location of Human Ortholog: 6q14-q21

Cellular Component: cell surface; membrane; cytoplasm; plasma membrane

Molecular Function: 5'-nucleotidase activity; metal ion binding; nucleotide binding

Biological Process: pyrimidine nucleoside catabolic process; pyrimidine base metabolic process; dephosphorylation; nucleobase, nucleoside and nucleotide metabolic process; adenosine biosynthetic process; negative regulation of inflammatory response; purine nucleotide catabolic process; DNA metabolic process; purine base metabolic process; AMP catabolic process

Disease: Calcification Of Joints And Arteries
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 Research Articles on NT5E    1. ecto-nucleotidases CD39 and CD73 are expressed in human endometrial tumors
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-NT5E antibodyPathways associated with anti-NT5E antibody
 Reference Product  PubMed Publications
 ENPP1 antibody  >4 publications with NT5E and ENPP1
 ADA antibody  >2 publications with NT5E and ADA
 ENTPD1 antibody  >2 publications with NT5E and ENTPD1
 Products by Pathway  Pathway Diagram
 HIF-1-alpha Transcription Factor Network Pathway antibodies  HIF-1-alpha Transcription Factor Network Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Nucleotides Pathway antibodies  Metabolism Of Nucleotides Pathway Diagram
 Nicotinate And Nicotinamide Metabolism Pathway antibodies  Nicotinate And Nicotinamide Metabolism Pathway Diagram
 Nicotinate And Nicotinamide Metabolism Pathway antibodies  Nicotinate And Nicotinamide Metabolism Pathway Diagram
 Purine Catabolism Pathway antibodies  Purine Catabolism Pathway Diagram
 Purine Metabolism Pathway antibodies  Purine Metabolism Pathway Diagram
 Purine Metabolism Pathway antibodies  Purine Metabolism Pathway Diagram
 Purine Metabolism Pathway antibodies  Purine Metabolism Pathway Diagram
Diseases associated with anti-NT5E antibodyOrgans/Tissues associated with anti-NT5E antibody
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >17 publications with NT5E and Inflammation
 Necrosis Antibodies  >9 publications with NT5E and Necrosis
 Breast Neoplasms Antibodies  >9 publications with NT5E and Breast Neoplasms
 Nervous System Diseases Antibodies  >9 publications with NT5E and Nervous System Diseases
 Disease Models, Animal Antibodies  >8 publications with NT5E and Disease Models, Animal
 Pain Antibodies  >7 publications with NT5E and Pain
 Brain Diseases Antibodies  >5 publications with NT5E and Brain Diseases
 Kidney Diseases Antibodies  >4 publications with NT5E and Kidney Diseases
 Heart Diseases Antibodies  >4 publications with NT5E and Heart Diseases
 Acute Kidney Injury Antibodies  >3 publications with NT5E and Acute Kidney Injury
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >33 publications with NT5E and Blood
 Vascular Antibodies  >20 publications with NT5E and Vascular
 Bone Antibodies  >13 publications with NT5E and Bone
 Brain Antibodies  >9 publications with NT5E and Brain
 Bone Marrow Antibodies  >8 publications with NT5E and Bone Marrow
 Muscle Antibodies  >8 publications with NT5E and Muscle
 Mouth Antibodies  >8 publications with NT5E and Mouth
 Kidney Antibodies  >6 publications with NT5E and Kidney
 Ganglia Antibodies  >6 publications with NT5E and Ganglia
 Embryonic Tissue Antibodies  >5 publications with NT5E and Embryonic Tissue
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