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anti-NEFM antibody :: Chicken anti-Rat Neurofilament NF-M ck Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS502143
Testing Data
Unit / Price
0.1 mL  /  $300 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Neurofilament NF-M ck (NEFM), Polyclonal Antibody

 Also Known As   

Anti-Neurofilament M (NF-M)

 Product Gene Name   

anti-NEFM antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Chromosome Location    Chromosome: 15; NC_005114.3 (46736787..46741632). Location: 15p12
 3D Structure    ModBase 3D Structure for P12839
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 Clonality    Polyclonal
 Host    Chicken
 Species Reactivity    Rat
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 Specificity    Specific for the ~145k Neurofilament M protein.
 Purity/Purification    Total IgY fraction
 Form/Format    Liquid containing 10mM sodium azide.
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 Antigen    Recombinant fusion protein containing the extreme C-terminus of rat NF-M expressed in and purified from E. coli.
 Immunogen Information    Preparation containing the extreme C-terminus expressed in and purified from E. Coli
 Immunogen Species    Rat
 Reactivity Assumed Based on 100% Sequence Homology    Chicken, human, mouse
 Species Reactivity Note    The antibody has been directly tested for reactivity in chicken, human, mouse and rat tissues.
 Biological Significance    Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H (1). NF-M is the neurofilament middle or medium molecular weight polypeptide and runs on SDS-PAGE gels at 145-160 kDa, with some variability across species boundaries. Antibodies to NF-M are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. NF-M antibodies can also be useful to visualize neurofilament accumulations seen in many neurological diseases, such as Amyotrophic Lateral Sclerosis (Lou Gehrig's disease) and Alzheimer's disease (2).
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 Preparation and Storage    Store at -20 degree C in undiluted aliquots; stable for at least 1 year. Avoid freeze/thaw cycles.
 Other Notes    Small volumes of anti-NEFM antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Product Description specifically for anti-NEFM antibody

   Chicken polyclonal antibody
 Applications Tested/Suitable for anti-NEFM antibody   

Western Blot (WB), Immunofluorescence (IF)

 Application Notes for anti-NEFM antibody    Quality Control: Western blots performed on each lot.
WB: 1:5,000
IF: 1:1000
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 Testing Data of anti-NEFM antibody    anti-NEFM antibody Testing Data image
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NCBI/Uniprot data below describe general gene information for NEFM. It may not necessarily be applicable to this product.
 NCBI GI #    8393823
 NCBI GeneID    24588
 NCBI Accession #    NP_058725.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_017029.2 [Other Products]
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 UniProt Primary Accession #    P12839 [Other Products]
 UniProt Secondary Accession #    Q63370 [Other Products]
 UniProt Related Accession #    P12839 [Other Products]
 Molecular Weight    145
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 NCBI Official Full Name    neurofilament medium polypeptide
 NCBI Official Synonym Full Names    neurofilament, medium polypeptide
 NCBI Official Symbol    Nefm [Similar Products]
 NCBI Official Synonym Symbols   
Nfm; Nef3
[Similar Products]
 NCBI Protein Information    neurofilament medium polypeptide; NF-M; neurofilament 3, medium; 160 kDa neurofilament protein; neurofilament triplet M protein; Neurofilament protein, middle polypeptide
 UniProt Protein Name    Neurofilament medium polypeptide
 UniProt Synonym Protein Names   
160 kDa neurofilament protein; Neurofilament 3; Neurofilament triplet M protein
 Protein Family    Neurofilament medium polypeptide
 UniProt Gene Name    Nefm [Similar Products]
 UniProt Synonym Gene Names    Nef3; Nfm; NF-M [Similar Products]
 UniProt Entry Name    NFM_RAT
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 NCBI Summary for NEFM    neuronal intermediate filament protein; important component of the cytoskeleton [RGD, Feb 2006]
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 UniProt Comments for NEFM    Function: Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.

Post-translational modification: There are a number of repeats of the tripeptide K-S-P, NFM is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFM results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber. Ref.4Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincidentally with a change in the neurofilament function.Phosphorylated in the head and rod regions by the PKC kinase PKN1, leading to the inhibition of polymerization

By similarity. Ref.4

Sequence similarities: Belongs to the intermediate filament family.
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Product References and Citations for anti-NEFM antibody

   • Harris, J., Ayyub, C. and Shaw G. (1991) A molecular dissection of the carboxyterminal tails of the major neurofilament subunits NF-M and NF-H. J Neurosci Res 30:47-62.
• Hu YY, He SS, Wang XC, Duan QH, Khatoon S, Igbal K, Grundke-Igbal I, Wang JZ (2002) Elevated levels of phosphorylated neurofilament proteins in cerebrospinal fluid of Alzheimer disease patients. Neurosci Lett 320(3):156-60.
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 Research Articles on NEFM    1. head domain phosphorylation of endogenous NF-M by forskolin-activated PKA inhibits NF-M tail domain phosphorylation in rat cortical neurons
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-NEFM antibodyPathways associated with anti-NEFM antibody
 Reference Product  PubMed Publications
 Nefl antibody  >2 publications with NEFM and Nefl
 Nes antibody  >1 publications with NEFM and Nes
 Products by Pathway  Pathway Diagram
 Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies  Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
 Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies  Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
Diseases associated with anti-NEFM antibodyOrgans/Tissues associated with anti-NEFM antibody
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >2 publications with NEFM and Neoplasms
 Nervous System Diseases Antibodies  >2 publications with NEFM and Nervous System Diseases
 Peripheral Nervous System Diseases Antibodies  >1 publications with NEFM and Peripheral Nervous System Diseases
 Brain Diseases Antibodies  >1 publications with NEFM and Brain Diseases
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >3 publications with NEFM and Brain
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