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| Product Name
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Neutrophil Elastase (ELANE), Polyclonal Antibody
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| Also Known As
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Sheep anti-human Neutrophil Elastase, Purified IgG
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| Product Synonym Names
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Neutrophil Elastase, human
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| Product Gene Name
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[Similar Products]
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| Research Use Only
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For Research Use Only. Not for use in diagnostic procedures.
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| Chromosome Location
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Chromosome: 19; NC_000019.9 (852291..856246). Location: 19p13.3
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| OMIM
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130130
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| 3D Structure
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ModBase 3D Structure for P08246
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| Clonality
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Polyclonal
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| Host
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Sheep
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| Specificity
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This antibody is specific for neutrophil elastase as demonstrated by immunoelectrophoresis and ELISA.
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| Purity/Purification
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Purified IgG
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| Immunogen
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Neutrophil elastase (active-site blocked) prepared from human sputum.
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| Presentation
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XXXX ml of immunoglobulin purified from sheep antiserum. The IgG concentration is XXXX mg/ml in 10 mM HEPES, pH 7.4, containing 0.15 M NaCl and 50% glycerol (v/v). Total protein is 10 mg.
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| Preparation and Storage
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Store between -10 and -20 degree C. Product will become viscous but will not freeze. Avoid storage in frost-free freezers. Keep vial tightly capped. Allow product to warm to room temperature and gently mix before use.
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| Other Notes
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Small volumes of anti-ELANE antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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| Application Notes for anti-ELANE antibody
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This product is suitable as a source of antibodies to neutrophil elastase.
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| NCBI/Uniprot data below describe general gene information for ELANE. It may not necessarily be applicable to this product. |
| NCBI GI #
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4503549
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| NCBI GeneID
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1991
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| NCBI Accession #
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NP_001963.1
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[Other Products]
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| NCBI GenBank Nucleotide #
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NM_001972.2
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[Other Products]
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| UniProt Primary Accession #
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P08246
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[Other Products]
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| UniProt Secondary Accession #
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P09649; Q6B0D9; Q6LDP5
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[Other Products]
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| UniProt Related Accession #
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P08246
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[Other Products]
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| Molecular Weight
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28,518 Da
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[Similar Products]
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| NCBI Official Full Name
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neutrophil elastase preproprotein
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| NCBI Official Synonym Full Names
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elastase, neutrophil expressed
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| NCBI Official Symbol
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ELANE
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[Similar Products]
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| NCBI Official Synonym Symbols
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GE; NE; HLE; HNE; ELA2; SCN1; PMN-E
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[Similar Products]
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| NCBI Protein Information
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neutrophil elastase; elastase-2; medullasin; PMN elastase; leukocyte elastase; elastase 2, neutrophil; human leukocyte elastase; polymorphonuclear elastase; bone marrow serine protease; granulocyte-derived elastase
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| UniProt Protein Name
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Neutrophil elastase
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| UniProt Synonym Protein Names
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Bone marrow serine protease; Elastase-2; Human leukocyte elastase; HLE; Medullasin; PMN elastase
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| Protein Family
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Neutrophil elastase
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| UniProt Gene Name
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ELANE
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[Similar Products]
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| UniProt Synonym Gene Names
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ELA2
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[Similar Products]
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| UniProt Entry Name
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ELNE_HUMAN
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| NCBI Summary for ELANE
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Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins. The product of this gene hydrolyzes proteins within specialized neutrophil lysosomes, called azurophil granules, as well as proteins of the extracellular matrix following the protein's release from activated neutrophils. The enzyme may play a role in degenerative and inflammatory diseases by its proteolysis of collagen-IV and elastin of the extracellular matrix. This protein degrades the outer membrane protein A (OmpA) of E. coli as well as the virulence factors of such bacteria as Shigella, Salmonella and Yersinia. Mutations in this gene are associated with cyclic neutropenia and severe congenital neutropenia (SCN). This gene is clustered with other serine protease gene family members, azurocidin 1 and proteinase 3 genes, at chromosome 19pter. All 3 genes are expressed coordinately and their protein products are packaged together into azurophil granules during neutrophil differentiation. [provided by RefSeq]
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| UniProt Comments for ELANE
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Function: Modifies the functions of natural killer cells, monocytes and granulocytes. Inhibits C5a-dependent neutrophil enzyme release and chemotaxis. Ref.15
Catalytic activity: Hydrolysis of proteins, including elastin. Preferential cleavage: Val-|-Xaa > Ala-|-Xaa.
Subunit structure: Interacts with NOTCH2NL. Ref.16
Tissue specificity: Bone marrow cells.
Involvement in disease: Defects in ELANE are a cause of cyclic haematopoiesis (CH) [
MIM:162800]; also known as cyclic neutropenia. CH is an autosomal dominant disease in which blood-cell production from the bone marrow oscillates with 21-day periodicity. Circulating neutrophils vary between almost normal numbers and zero. During intervals of neutropenia, affected individuals are at risk for opportunistic infection. Monocytes, platelets, lymphocytes and reticulocytes also cycle with the same frequency. Ref.16 Ref.21Defects in ELANE are the cause of neutropenia severe congenital autosomal dominant type 1 (SCN1) [
MIM:202700]. SCN1 is a disorder of hematopoiesis characterized by a maturation arrest of granulopoiesis at the level of promyelocytes with peripheral blood absolute neutrophil counts below 0.5 x 109/l and early onset of severe bacterial infections. Ref.26
Sequence similarities: Belongs to the peptidase S1 family. Elastase subfamily.Contains 1 peptidase S1 domain.
Sequence caution: The sequence CAA29300.1 differs from that shown. Reason: Erroneous initiation.
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| Research Articles on ELANE
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1. The study shows that the alternative pathway for plasmin-mediated fibrinolysis by leukocyte elastase is activated, and contributes to the degradation of cross-linked fibrin in sepsis-induced disseminated intravascular coagulation.
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| Precautions
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All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
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| Disclaimer
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While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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| Products associated with anti-ELANE antibody | Pathways associated with anti-ELANE antibody |
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| Diseases associated with anti-ELANE antibody | Organs/Tissues associated with anti-ELANE antibody |
| Disease Name |
Pubmed Publications |
| Cyclic neutropenia Antibodies |
>6 publications with ELANE and Cyclic neutropenia |
| NEUTROPENIA, SEVERE CONGENITAL, 1, AUTOSOMAL DOMINANT Antibodies |
>4 publications with ELANE and NEUTROPENIA, SEVERE CONGENITAL, 1, AUTOSOMAL DOMINANT |
| Neoplasms Antibodies |
>2 publications with ELANE and Neoplasms |
| Bacterial Infections Antibodies |
>2 publications with ELANE and Bacterial Infections |
| Lung Neoplasms Antibodies |
>1 publications with ELANE and Lung Neoplasms |
| Leukemia, Myeloid, Acute Antibodies |
>1 publications with ELANE and Leukemia, Myeloid, Acute |
| Osteoporosis Antibodies |
>1 publications with ELANE and Osteoporosis |
| Anemia Antibodies |
>1 publications with ELANE and Anemia |
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Antibody 
