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anti-NOTCH1 antibody :: Rabbit anti-Human, Mouse Notch1, Cleaved Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS618561 anti-NOTCH1 antibody
Unit / Price
0.1 mL  /  $485 +1 FREE 8GB USB
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Notch1, Cleaved, Polyclonal Antibody

 Also Known As   

Notch1, Cleaved, NT (Notch Homolog Protein 1, hN1, Translocation-associated Notch Protein TAN-1)

 Product Synonym Names    Anti -Notch1, Cleaved, NT (Notch Homolog Protein 1, hN1, Translocation-associated Notch Protein TAN-1)
 Product Gene Name   

anti-NOTCH1 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Chromosome Location    Chromosome: 9; NC_000009.11 (139388896..139440238, complement). Location: 9q34.3
 OMIM    109730
 3D Structure    ModBase 3D Structure for P46531
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 Clonality    Polyclonal
 Isotype    IgG
 Host    Rabbit
 Species Reactivity    Human, Mouse
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 Specificity    Recognizes human NOTCH 1. Species Crossreactivity
 Purity/Purification    Serum
 Form/Format    Supplied as liquid in PBS, 0.1% sodium azide. No stabilizing proteins added.
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 Immunogen    Synthetic peptide, aa1755-1767 VLLSRKRRRQHGQC, corresponding to human Notch 1. This is the N-terminal sequence of the cleaved N intracellular domain (NICD).
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 Preparation and Storage    May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
 Other Notes    Small volumes of anti-NOTCH1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Product Description specifically for anti-NOTCH1 antibody

   Notch is synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase (S1 cleavage) in the trans-golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved (S2 cleavage) by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin-dependent gamma-secretase (S3 cleavage) to release the intracellular domain (NICD) from the membrane.
 Product Categories/Family for anti-NOTCH1 antibody    Antibodies; Abs to Notch Proteins
 Applications Tested/Suitable for anti-NOTCH1 antibody   

ELISA (EL/EIA), Western Blot (WB)

 Application Notes for anti-NOTCH1 antibody    Suitable for use in ELISA and Western Blot.
Dilution: ELISA: 1:20,000-1:60,000. Standard capture ELISA using IgG, H&L (HRP) Pab Gt xRb and ABTS (A0030) as a substrate for 30 minutes at RT.
Western Blot: 1:500- 1:2000. Against recombinant mouse Notch constructs in 293 cells. An 80kD band corresponding to Notch 1.
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NCBI/Uniprot data below describe general gene information for NOTCH1. It may not necessarily be applicable to this product.
 NCBI GI #    148833508
 NCBI GeneID    4851
 NCBI Accession #    NP_060087.3 [Other Products]
 NCBI GenBank Nucleotide #    NM_017617.3 [Other Products]
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 UniProt Primary Accession #    P46531 [Other Products]
 UniProt Secondary Accession #    Q59ED8; Q5SXM3 [Other Products]
 UniProt Related Accession #    P46531; Q6IAD4; Q9NXX4 [Other Products]
 Molecular Weight    272,505 Da [Similar Products]
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 NCBI Official Full Name    neurogenic locus notch homolog protein 1 preproprotein
 NCBI Official Synonym Full Names    notch 1
 NCBI Official Symbol    NOTCH1 [Similar Products]
 NCBI Official Synonym Symbols   
hN1; TAN1
[Similar Products]
 NCBI Protein Information    neurogenic locus notch homolog protein 1; OTTHUMP00000022594; Notch homolog 1, translocation-associated; translocation-associated notch protein TAN-1
 UniProt Protein Name    Neurogenic locus notch homolog protein 1
 UniProt Synonym Protein Names   
Translocation-associated notch protein TAN-1
 Protein Family    Neurogenic locus notch homolog protein
 UniProt Gene Name    NOTCH1 [Similar Products]
 UniProt Synonym Gene Names    TAN1 [Similar Products]
 UniProt Entry Name    NOTC1_HUMAN
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 NCBI Summary for NOTCH1    This gene encodes a member of the Notch family. Members of this Type 1 transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple, different domain types. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. The Notch signaling network is an evolutionarily conserved intercellular signaling pathway which regulates interactions between physically adjacent cells. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signaling pathway that plays a key role in development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remain to be determined. This protein is cleaved in the trans-Golgi network, and presented on the cell surface as a heterodimer. This protein functions as a receptor for membrane bound ligands, and may play multiple roles during development. [provided by RefSeq]
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 UniProt Comments for NOTCH1    Notch 1: Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. May be important for normal lymphocyte function. In altered form, may contribute to transformation or progression in some T-cell neoplasms. Involved in the maturation of both CD4+ and CD8+ cells in the thymus. May be important for follicular differentiation and possibly cell fate selection within the follicle. During cerebellar development, may function as a receptor for neuronal DNER and may be involved in the differentiation of Bergmann glia. Represses neuronal and myogenic differentiation. May enhance HIF1A function by sequestering HIF1AN away from HIF1A. Heterodimer of a C-terminal fragment N(TM) and an N- terminal fragment N(EC) which are probably linked by disulfide bonds. Interacts with DNER, DTX1, DTX2 and RBPJ/RBPSUH. Also interacts with MAML1, MAML2 and MAML3 which act as transcriptional coactivators for NOTCH1. The activated membrane-bound form interacts with AAK1 which promotes NOTCH1 stabilization. Forms a trimeric complex with FBXW7 and SGK1. Interacts with HIF1AN. HIF1AN negatively regulates the function of notch intracellular domain (NICD), accelerating myogenic differentiation. In fetal tissues most abundant in spleen, brain stem and lung. Also present in most adult tissues where it is found mainly in lymphoid tissues. Belongs to the NOTCH family.

Protein type: Motility/polarity/chemotaxis; Receptor, misc.; Membrane protein, integral; Oncoprotein; Transcription factor

Chromosomal Location of Human Ortholog: 9q34.3

Cellular Component: Golgi membrane; nucleoplasm; endoplasmic reticulum membrane; cell surface; extracellular region; plasma membrane; integral to membrane; acrosome; cytosol; nucleus; receptor complex

Molecular Function: enzyme inhibitor activity; protein binding; enzyme binding; chromatin DNA binding; sequence-specific DNA binding; calcium ion binding; receptor activity; transcription factor activity

Biological Process: neural tube development; negative regulation of calcium ion-dependent exocytosis; positive regulation of apoptosis; positive regulation of transcription, DNA-dependent; heart development; positive regulation of JAK-STAT cascade; response to lipopolysaccharide; cell differentiation in spinal cord; response to corticosteroid stimulus; negative regulation of BMP signaling pathway; compartment specification; positive regulation of endothelial cell differentiation; negative regulation of ossification; oligodendrocyte differentiation; somatic stem cell division; negative regulation of osteoblast differentiation; positive regulation of keratinocyte differentiation; positive regulation of cardiac muscle cell proliferation; positive regulation of astrocyte differentiation; negative regulation of photoreceptor cell differentiation; positive regulation of neuroblast proliferation; organ regeneration; Notch receptor processing; keratinocyte differentiation; branching morphogenesis of a tube; response to muramyl dipeptide; positive regulation of transcription from RNA polymerase II promoter; determination of left/right symmetry; negative regulation of transcription, DNA-dependent; foregut morphogenesis; positive regulation of epithelial cell proliferation; endoderm development; cardiac muscle cell proliferation; cell fate specification; negative regulation of transcription from RNA polymerase II promoter; embryonic hindlimb morphogenesis; negative regulation of neurogenesis; negative regulation of cell proliferation; astrocyte differentiation; regulation of transcription, DNA-dependent; tissue regeneration; cardiac muscle morphogensis; positive regulation of cell proliferation; forebrain development; heart looping; regulation of somitogenesis; positive regulation of BMP signaling pathway; mesenchymal cell development; transcription initiation from RNA polymerase II promoter; Notch signaling pathway; hair follicle morphogenesis; in utero embryonic development; lumen formation; liver development; humoral immune response; activation of Notch receptor target transcription factor; negative regulation of oligodendrocyte differentiation; inflammatory response to antigenic stimulus; axonogenesis; negative regulation of catalytic activity; epithelial to mesenchymal transition; spermatogenesis; immune response; gene expression; sprouting angiogenesis; negative regulation of myoblast differentiation; auditory receptor cell fate commitment; lung development; positive regulation of cell migration

Disease: Adams-oliver Syndrome 5; Aortic Valve Disease 1
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 Research Articles on NOTCH1    1. Notch-1 signaling is involved in the upregulation of survivin expression in lung cancer cells, which is synergized by HIF-1alpha.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-NOTCH1 antibodyPathways associated with anti-NOTCH1 antibody
 Reference Product  PubMed Publications
 RBPJ antibody  >111 publications with NOTCH1 and RBPJ
 DLL4 antibody  >84 publications with NOTCH1 and DLL4
 DLL1 antibody  >73 publications with NOTCH1 and DLL1
 JAG2 antibody  >58 publications with NOTCH1 and JAG2
 JAG1 antibody  >49 publications with NOTCH1 and JAG1
 FBXW7 antibody  >49 publications with NOTCH1 and FBXW7
 NUMB antibody  >46 publications with NOTCH1 and NUMB
 PSEN1 antibody  >40 publications with NOTCH1 and PSEN1
 MAML1 antibody  >34 publications with NOTCH1 and MAML1
 LFNG antibody  >33 publications with NOTCH1 and LFNG
 Products by Pathway  Pathway Diagram
 A Third Proteolytic Cleavage Releases NICD Pathway antibodies  A Third Proteolytic Cleavage Releases NICD Pathway Diagram
 Delta-Notch Signaling Pathway antibodies  Delta-Notch Signaling Pathway Diagram
 Dorso-ventral Axis Formation Pathway antibodies  Dorso-ventral Axis Formation Pathway Diagram
 Dorso-ventral Axis Formation Pathway antibodies  Dorso-ventral Axis Formation Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 Generic Transcription Pathway antibodies  Generic Transcription Pathway Diagram
 Heart Development Pathway antibodies  Heart Development Pathway Diagram
 Maturation Of Notch Precursor Via Proteolytic Cleavage Pathway antibodies  Maturation Of Notch Precursor Via Proteolytic Cleavage Pathway Diagram
 Mature Notch Receptor Trafficks To Plasma Membrane Pathway antibodies  Mature Notch Receptor Trafficks To Plasma Membrane Pathway Diagram
 NICD Trafficks To Nucleus Pathway antibodies  NICD Trafficks To Nucleus Pathway Diagram
Diseases associated with anti-NOTCH1 antibodyOrgans/Tissues associated with anti-NOTCH1 antibody
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >644 publications with NOTCH1 and Neoplasms
 Leukemia Antibodies  >314 publications with NOTCH1 and Leukemia
 Leukemia, T-Cell Antibodies  >232 publications with NOTCH1 and Leukemia, T-Cell
 Lymphoma Antibodies  >224 publications with NOTCH1 and Lymphoma
 Lymphoma, T-Cell Antibodies  >185 publications with NOTCH1 and Lymphoma, T-Cell
 Carcinoma Antibodies  >155 publications with NOTCH1 and Carcinoma
 Cardiovascular Diseases Antibodies  >102 publications with NOTCH1 and Cardiovascular Diseases
 Cell Transformation, Neoplastic Antibodies  >102 publications with NOTCH1 and Cell Transformation, Neoplastic
 Congenital Abnormalities Antibodies  >56 publications with NOTCH1 and Congenital Abnormalities
 Chromosome Aberrations Antibodies  >51 publications with NOTCH1 and Chromosome Aberrations
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >407 publications with NOTCH1 and Blood
 Brain Antibodies  >231 publications with NOTCH1 and Brain
 Embryonic Tissue Antibodies  >214 publications with NOTCH1 and Embryonic Tissue
 Bone Antibodies  >208 publications with NOTCH1 and Bone
 Vascular Antibodies  >171 publications with NOTCH1 and Vascular
 Bone Marrow Antibodies  >137 publications with NOTCH1 and Bone Marrow
 Muscle Antibodies  >135 publications with NOTCH1 and Muscle
 Thymus Antibodies  >120 publications with NOTCH1 and Thymus
 Heart Antibodies  >86 publications with NOTCH1 and Heart
 Skin Antibodies  >79 publications with NOTCH1 and Skin
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