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anti-ORAI1 antibody :: Rabbit anti-Mouse, Rat ORAI1 Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS222945
Unit / Price
0.1 mg  /  $390 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

ORAI1, Polyclonal Antibody

 Also Known As   

RABBIT ANTI ORAI1 (C-TERMINAL)

 Product Gene Name   

anti-ORAI1 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    610277
 3D Structure    ModBase 3D Structure for Q96D31
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 Clonality    Polyclonal
 Isotype    IgG
 Host    Rabbit
 Species Reactivity    Mouse, Rat
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 Specificity    This item recognises human ORAI1, also known as Transmembrane protein 142A (TMEM142A), a 32.7 kDa multi-pass membrane protein belonging to the ORAI family.

Antigen stimulation of immune cells triggers Ca2+ entry through Ca2+ release-activated Ca2+ (CRAC) channels. CRAC channels are the main pathway for Ca2+ influx in T-cells and promote the immune response to pathogens by activating the transcription factor NFAT.

ORAI1 is a four- transmembrane spanning protein that is an essential component of CRAC. ORAI1 functions as a highly selective Ca2+ plasma membrane channel that is gated through interactions with STIM1, the store-activated endoplasmic reticulum Ca2+ sensor.

Defective ORAI1 in humans is the cause of one form of hereditary severe combined immune deficiency (SCID), which results in ablated T-cell Ca2+ entry.

This item is not expected to cross react with either ORAI2 or ORAI3.
 Form/Format    Purified
Purified IgG - liquid
 Concentration    IgG concentration 1.0mg/ml (lot specific)
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 Perservative Stabilisers    0.02% Sodium Azide (NaN3)
 Immunogen    A 16 amino acid peptide from near the carboxy terminus of human ORAI1.
Buffer Solution
 Antiserum Preparation    Antiserum to human ORAI1 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
 Target Species    Human
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 Preparation and Storage    Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
 Other Notes    Small volumes of anti-ORAI1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Product Description specifically for anti-ORAI1 antibody

   MBS222945 recognises human ORAI1, also known as Transmembrane protein 142A (TMEM142A), a 32.7 kDa multi-pass membrane protein belonging to the ORAI family. Antigen stimulation of immune cells triggers Ca2+ entry through Ca2+ release-activated Ca2+ (CRAC) channels. CRAC channels are the main pathway for Ca2+ influx in T-cells and promote the immune response to pathogens by activating the transcription factor NFAT. ORAI1 is a four- transmembrane spanning protein that is an essential component of CRAC. ORAI1 functions as a highly selective Ca2+ plasma membrane channel that is gated through interactions with STIM1, the store-activated endoplasmic reticulum Ca2+ sensor. Defective ORAI1 in humans is the cause of one form of hereditary severe combined immune deficiency (SCID), which results in ablated T-cell Ca2+ entry. MBS222945 is not expected to cross react with either ORAI2 or ORAI3.
 Applications Tested/Suitable for anti-ORAI1 antibody   

Immunohistology Paraffin*, Western Blot (WB)

 Application Notes for anti-ORAI1 antibody    Western Blot: This item detects a band of approximately 49kDa in 49 cell lysates.
Immunohistology - Paraffin: Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.

Western Blotting: Minimum Dilution: 0.5; Maximum Dilution: 1ug/ml
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 Testing Data #1 of anti-ORAI1 antibody    Western blot analysis of human ovary probed with Rabbit anti Human ORAI1 (MBS222945) at 0.5(A), 1(B) and 2(C) ug/ml
anti-ORAI1 antibody Testing Data #1 image
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 Testing Data #2 of anti-ORAI1 antibody    Formalin fixed, paraffin embedded human spleen stained with Rabbit anti Human ORAI1 (MBS222945) following heat mediated antigen retrieval using sodium citrate buffer (pH6.0)
anti-ORAI1 antibody Testing Data #2 image
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NCBI/Uniprot data below describe general gene information for ORAI1. It may not necessarily be applicable to this product.
 NCBI GI #    38016943
 NCBI GeneID    84876
 NCBI Accession #    NP_116179.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_032790.3 [Other Products]
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 UniProt Primary Accession #    Q96D31 [Other Products]
 UniProt Secondary Accession #    Q3MHV3; Q6DHX2; Q96BP7; Q96K71 [Other Products]
 UniProt Related Accession #    Q96D31 [Other Products]
 Molecular Weight    25,337 Da
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 NCBI Official Full Name    calcium release-activated calcium channel protein 1
 NCBI Official Synonym Full Names    ORAI calcium release-activated calcium modulator 1
 NCBI Official Symbol    ORAI1 [Similar Products]
 NCBI Official Synonym Symbols   
IMD9; TAM2; ORAT1; CRACM1; TMEM142A
[Similar Products]
 NCBI Protein Information    calcium release-activated calcium channel protein 1; calcium release-activated calcium modulator 1; protein orai-1; transmembrane protein 142A
 UniProt Protein Name    Calcium release-activated calcium channel protein 1
 UniProt Synonym Protein Names   
Protein orai-1; Transmembrane protein 142A
 UniProt Gene Name    ORAI1 [Similar Products]
 UniProt Synonym Gene Names    CRACM1; TMEM142A [Similar Products]
 UniProt Entry Name    CRCM1_HUMAN
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 NCBI Summary for ORAI1    The protein encoded by this gene is a membrane calcium channel subunit that is activated by the calcium sensor STIM1 when calcium stores are depleted. This type of channel is the primary way for calcium influx into T-cells. Defects in this gene are a cause of immune dysfunction with T-cell inactivation due to calcium entry defect type 1 (IDTICED1). [provided by RefSeq, Sep 2011]
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 UniProt Comments for ORAI1    ORAI1: Ca(2+) release-activated Ca(2+) (CRAC) channel subunit which mediates Ca(2+) influx following depletion of intracellular Ca(2+) stores and channel activation by the Ca(2+) sensor, STIM1. CRAC channels are the main pathway for Ca(2+) influx in T-cells and promote the immune response to pathogens by activating the transcription factor NFAT. Interacts with STIM1 and STIM2. Interacts with EFCAB4B/CRACR2A; the interaction is direct and takes place in absence of Ca(2+). Forms a complex with EFCAB4B/CRACR2A and STIM1 at low concentration of Ca(2+), the complex dissociates at elevated Ca(2+) concentrations. Belongs to the Orai family.

Protein type: Channel, calcium; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 12q24.31

Cellular Component: protein complex; membrane; integral to plasma membrane

Molecular Function: calmodulin binding; identical protein binding; protein binding; store-operated calcium channel activity

Biological Process: immune system process; regulation of calcium ion transport; positive regulation of calcium ion transport; blood coagulation

Disease: Myopathy, Tubular Aggregate, 2; Immunodeficiency 9
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 Research Articles on ORAI1    1. Data show that increased activity of STIM1-Orai1 complex inducing enhanced Ca(2+) influx is a disease determinant in muscular dystrophy.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-ORAI1 antibodyPathways associated with anti-ORAI1 antibody
 Reference Product  PubMed Publications
 STIM1 antibody  >582 publications with ORAI1 and STIM1
 TRPC1 antibody  >97 publications with ORAI1 and TRPC1
 STIM2 antibody  >85 publications with ORAI1 and STIM2
 TRPC6 antibody  >32 publications with ORAI1 and TRPC6
 TRPC3 antibody  >31 publications with ORAI1 and TRPC3
 CCND1 antibody  >3 publications with ORAI1 and CCND1
 ITPR3 antibody  >2 publications with ORAI1 and ITPR3
 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Antigen Activates B Cell Receptor (BCR) Leading To Generation Of Second Messengers Pathway antibodies  Antigen Activates B Cell Receptor (BCR) Leading To Generation Of Second Messengers Pathway Diagram
 Calcium Signaling Pathway antibodies  Calcium Signaling Pathway Diagram
 Calcium Signaling Pathway antibodies  Calcium Signaling Pathway Diagram
 Elevation Of Cytosolic Ca2+ Levels Pathway antibodies  Elevation Of Cytosolic Ca2+ Levels Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Platelet Activation Pathway antibodies  Platelet Activation Pathway Diagram
 Platelet Calcium Homeostasis Pathway antibodies  Platelet Calcium Homeostasis Pathway Diagram
 Platelet Homeostasis Pathway antibodies  Platelet Homeostasis Pathway Diagram
Diseases associated with anti-ORAI1 antibodyOrgans/Tissues associated with anti-ORAI1 antibody
 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >35 publications with ORAI1 and Cardiovascular Diseases
 Inflammation Antibodies  >33 publications with ORAI1 and Inflammation
 Nervous System Diseases Antibodies  >25 publications with ORAI1 and Nervous System Diseases
 Disease Models, Animal Antibodies  >23 publications with ORAI1 and Disease Models, Animal
 Necrosis Antibodies  >17 publications with ORAI1 and Necrosis
 Breast Neoplasms Antibodies  >14 publications with ORAI1 and Breast Neoplasms
 Kidney Diseases Antibodies  >12 publications with ORAI1 and Kidney Diseases
 Hypertension Antibodies  >11 publications with ORAI1 and Hypertension
 Hypertrophy Antibodies  >8 publications with ORAI1 and Hypertrophy
 Hyperplasia Antibodies  >7 publications with ORAI1 and Hyperplasia
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >160 publications with ORAI1 and Blood
 Kidney Antibodies  >40 publications with ORAI1 and Kidney
 Brain Antibodies  >37 publications with ORAI1 and Brain
 Heart Antibodies  >37 publications with ORAI1 and Heart
 Lung Antibodies  >31 publications with ORAI1 and Lung
 Bone Antibodies  >24 publications with ORAI1 and Bone
 Skin Antibodies  >12 publications with ORAI1 and Skin
 Bone Marrow Antibodies  >11 publications with ORAI1 and Bone Marrow
 Embryonic Tissue Antibodies  >11 publications with ORAI1 and Embryonic Tissue
 Salivary Gland Antibodies  >10 publications with ORAI1 and Salivary Gland
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