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anti-OCLN antibody :: Rabbit Occludin Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS621431 anti-OCLN antibody
Unit / Price
0.1 mg  /  $620 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Occludin (OCLN), Polyclonal Antibody

 Also Known As   

Occludin

 Product Synonym Names    Anti -Occludin
 Product Gene Name   

anti-OCLN antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Chromosome Location    Chromosome: 5; NC_000005.9 (68788119..68850397). Location: 5q13.1
 OMIM    251290
 3D Structure    ModBase 3D Structure for Q16625
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 Clonality    Polyclonal
 Isotype    IgG
 Host    Rabbit
 Species Reactivity    Canine, Human, Mammal, Mouse, Rat
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 Specificity    Recognizes human occludin at ~65kD. Reactivity of this antibody with the occludin protein has been confirmed by Western Blot using total cell lysates derived from MDCK cells, human T84 cells, human Caco-2 and rat fibroblasts transfected with a plasmid encoding human occludin. Species Crossreactivity: mammalian, mouse, rat and canine
 Purity/Purification    Affinity Purified
Purified by immunoaffinity chromatography.
 Form/Format    Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
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 Immunogen    Fusion protein containing the C-terminal 150aa of human occludin.
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 Preparation and Storage    May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
 Other Notes    Small volumes of anti-OCLN antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Product Categories/Family for anti-OCLN antibody    Antibodies; Abs to Signal Transduction
 Applications Tested/Suitable for anti-OCLN antibody   

ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunofluorescence (IF)

 Application Notes for anti-OCLN antibody    Suitable for use in ELISA, Western Blot, Immunofluorescence, Immunoprecipitation and Immunohistochemistry.
Dilution: ELISA: 0.1-1ug/ml
Western Blot: 1-3ug/ml
Immunofluorescence: 4-20ug/ml
Immunoprecipitation: ~5ug/IP reaction
Immunohistochemistry (FFPE): 2-5ug/ml; Protease pretreatment of sections is required prior to staining. Dilute protease to 1-2mg/ml and incubate at 37 degree C for 10 minutes. Other enzyme and HIER pretreatment methods may not be suitable for this antibody.
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NCBI/Uniprot data below describe general gene information for OCLN. It may not necessarily be applicable to this product.
 NCBI GI #    4505487
 NCBI GeneID    100506658
 NCBI Accession #    NP_002529.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_002538.3 [Other Products]
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 UniProt Primary Accession #    Q16625 [Other Products]
 UniProt Secondary Accession #    Q8N6K1; B5BU70 [Other Products]
 UniProt Related Accession #    Q16625; Q5U1V4 [Other Products]
 Molecular Weight    59,144 Da [Similar Products]
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 NCBI Official Full Name    occludin isoform a
 NCBI Official Synonym Full Names    occludin
 NCBI Official Symbol    OCLN [Similar Products]
 NCBI Official Synonym Symbols   
BLCPMG; FLJ08163; FLJ18079; FLJ77961; FLJ94056; MGC34277
[Similar Products]
 NCBI Protein Information    occludin; OTTHUMP00000125187; OTTHUMP00000222347; tight junction protein occludin
 UniProt Protein Name    Occludin
 Protein Family    Occludin
 UniProt Gene Name    OCLN [Similar Products]
 UniProt Entry Name    OCLN_HUMAN
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 NCBI Summary for OCLN    This gene encodes an integral membrane protein that is required for cytokine-induced regulation of the tight junction paracellular permeability barrier. Mutations in this gene are thought to be a cause of band-like calcification with simplified gyration and polymicrogyria (BLC-PMG), an autosomal recessive neurologic disorder that is also known as pseudo-TORCH syndrome. Alternative splicing results in multiple transcript variants. A related pseudogene is present 1.5 Mb downstream on the q arm of chromosome 5. [provided by RefSeq]
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 UniProt Comments for OCLN    occludin: May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. It is able to induce adhesion when expressed in cells lacking tight junctions. Interacts with TJP1/ZO1 and with VAPA. Localized at tight junctions of both epithelial and endothelial cells. Highly expressed in kidney. Not detected in testis. Belongs to the ELL/occludin family. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Motility/polarity/chemotaxis; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 5q13.1

Cellular Component: tight junction; endocytic vesicle; apicolateral plasma membrane; apical plasma membrane; plasma membrane; integral to membrane; cytoplasmic vesicle; intercellular junction; cytosol; cell junction

Molecular Function: thiopurine S-methyltransferase activity; protein domain specific binding; protein binding; structural molecule activity

Biological Process: methylation; intercellular junction assembly and maintenance; apoptosis; S-adenosylmethionine metabolic process; protein complex assembly; S-adenosylhomocysteine metabolic process; cell structure disassembly during apoptosis

Disease: Band-like Calcification With Simplified Gyration And Polymicrogyria
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 Research Articles on OCLN    1. occludin S408 dephosphorylation regulates paracellular permeability by remodeling tight junction protein dynamic behavior and intermolecular interactions between occludin, ZO-1, and select claudinszo
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-OCLN antibodyPathways associated with anti-OCLN antibody
 Reference Product  PubMed Publications
 TJP1 antibody  >3 publications with OCLN and TJP1
 Products by Pathway  Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Hepatitis C Pathway antibodies  Hepatitis C Pathway Diagram
 Hepatitis C Pathway antibodies  Hepatitis C Pathway Diagram
 Leukocyte Transendothelial Migration Pathway antibodies  Leukocyte Transendothelial Migration Pathway Diagram
 Leukocyte Transendothelial Migration Pathway antibodies  Leukocyte Transendothelial Migration Pathway Diagram
 Pathogenic Escherichia Coli Infection Pathway antibodies  Pathogenic Escherichia Coli Infection Pathway Diagram
 Pathogenic Escherichia Coli Infection Pathway antibodies  Pathogenic Escherichia Coli Infection Pathway Diagram
 TGF-beta Receptor Signaling Pathway antibodies  TGF-beta Receptor Signaling Pathway Diagram
 Tight Junction Pathway antibodies  Tight Junction Pathway Diagram
Diseases associated with anti-OCLN antibodyOrgans/Tissues associated with anti-OCLN antibody
 Disease Name  Pubmed Publications
 Hepatitis Antibodies  >9 publications with OCLN and Hepatitis
 Liver Diseases Antibodies  >5 publications with OCLN and Liver Diseases
 Neoplasms Antibodies  >5 publications with OCLN and Neoplasms
 Liver Neoplasms Antibodies  >4 publications with OCLN and Liver Neoplasms
 Leukemia Antibodies  >3 publications with OCLN and Leukemia
 Central Nervous System Diseases Antibodies  >3 publications with OCLN and Central Nervous System Diseases
 Cardiovascular Diseases Antibodies  >2 publications with OCLN and Cardiovascular Diseases
 Carcinoma, Hepatocellular Antibodies  >2 publications with OCLN and Carcinoma, Hepatocellular
 Nervous System Malformations Antibodies  >2 publications with OCLN and Nervous System Malformations
 Vascular Diseases Antibodies  >2 publications with OCLN and Vascular Diseases
 Organ/Tissue Name  Pubmed Publications
 Testis Antibodies  >1 publications with OCLN and Testis
 Lung Antibodies  >1 publications with OCLN and Lung
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