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anti-OPRM1 antibody :: Opioid Receptor Mu IgG fraction Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS352201 anti-OPRM1 antibody
Unit / Price
0.1 mL  /  $700 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Opioid Receptor Mu IgG fraction (OPRM1), Polyclonal Antibody

 Also Known As   

Opioid Receptor Mu IgG fraction

 Product Synonym Names    Opioid Mu Receptor (391-398); IgG Fraction of Rabbit Anti-Mu Opioid Receptor Serum
 Product Gene Name   

anti-OPRM1 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Chromosome Location    Chromosome: 6; NC_000006.11 (154331636..154568001). Location: 6q24-q25
 OMIM    600018
 3D Structure    ModBase 3D Structure for P35372
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 Clonality    Polyclonal
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 Specificity    Polypeptide ::: % Cross Reactivity
Mu Opioid Receptor (391-398) ::: 100
Mu Opioid Receptor ::: 50
 Form/Format    Antiserum Specificity
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 Other Notes    Small volumes of anti-OPRM1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-OPRM1 antibody

   This IgG fraction was prepared by precipitation. dialysis. and chromatography from an antiserum that was raised a rabbit which was immunized with a peptide analogue of the carboxyl terminal of the mu opioid receptor covalently attached onto a carrier protein. This IgG fraction has been shown to be specific for the COOH terminal of the mu opioid receptor. is suitable for immunocytochemical and western immunoblotting detection of the receptor. and has been characterized by western blotting. ELISA. and cell staining techniques. Dilute the lyophilized antiserum with 0.1 ml of 10 mg/ml BSA is PBS for the equivalent of whole antiserum. or with additional buffer for more dilute antiserum. This vial contains 2.14 mg of purified IgG
 Product Categories/Family for anti-OPRM1 antibody    Histochemical Grade Antibodies; Opioid Receptor Antibodies and Kits
 Applications Tested/Suitable for anti-OPRM1 antibody   

Immunocytochemical Staining, Western Immunoblot, Western Blot

 Application Notes for anti-OPRM1 antibody    Immunocytochemistry: This IgG fraction has been found to stain specific cells in various regions of PLP fixed rat brain sections known to express the mu opioid receptor using ABC techniques at a concentration of 20 ug per ml.

Western Blot: Western immunoblots using whole rat brain homogenate have been successful at a concentration of 50 ug per ml.

Western Blot Protocol: 1. After SDS-PAGE (on either 4-15% gradient gels or single percentage gels. such at 12% gels) and electrophoretic transfer to PVDF membrane. block the membrane overnight with 4% normal goat serum in TBS/Tween-20 buffer.
2. Wash x 2 with TBS/Tween-20.
3. Apply the IgG fraction after dilution to at least 50 ugm per ml (Note: higher dilutions may be needed). Use 2% normal goat serum in TBS/Tween-20 as buffer for the primary antibody. Let the primary antibody bind for 2-4 hours.
4. Wash x 3 with TBS/Tween-20.
5. Apply affinity purified HRP-goat anti-rabbit IgG antiserum diluted 1:2500 (dilution may vary depending upon supplier) in 2% normal goat serum in TBS/Tween-20. Incubate 1-2 hours. Note: greater sensitivity may be achieved using ABC techniques.
6. Wash x 4 for 5 minute/wash with TBS/Tween-20.
7. Develop color using the enhanced DAB reaction.
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NCBI/Uniprot data below describe general gene information for OPRM1. It may not necessarily be applicable to this product.
 NCBI GI #    117940060
 NCBI GeneID    4988
 NCBI Accession #    NP_000905.3 [Other Products]
 NCBI GenBank Nucleotide #    NM_000914.2 [Other Products]
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 UniProt Primary Accession #    P35372 [Other Products]
 UniProt Secondary Accession #    Q12930; Q4VWM1; Q4VWM2; Q4VWM3; Q4VWM4; Q4VWM6; Q4VWX6; Q5TDA1; Q6UQ80; B2R9S7; B8Q1L7 [Other Products]
 UniProt Related Accession #    O43185; P35372; Q6UPP1; Q716A7; Q71V90; Q7Z6A5; Q86V80 [Other Products]
 Molecular Weight    44648 Da [Similar Products]
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 NCBI Official Full Name    mu-type opioid receptor isoform MOR-1
 NCBI Official Synonym Full Names    opioid receptor, mu 1
 NCBI Official Symbol    OPRM1 [Similar Products]
 NCBI Official Synonym Symbols   
MOR; LMOR; MOR1; OPRM; KIAA0403
[Similar Products]
 NCBI Protein Information    mu-type opioid receptor; MOP; hMOP; M-OR-1; OTTHUMP00000017463; OTTHUMP00000017464; OTTHUMP00000225129; OTTHUMP00000225131; OTTHUMP00000225132; OTTHUMP00000225133; OTTHUMP00000225135; OTTHUMP00000225136; OTTHUMP00000225139; OTTHUMP00000225164; OTTHUMP00000225165; OTTHUMP00000225166; OTTHUMP00000225167; OTTHUMP00000225168; OTTHUMP00000225169; OTTHUMP00000225170; mu opiate receptor
 UniProt Protein Name    Mu-type opioid receptor
 UniProt Synonym Protein Names   
Mu opiate receptor; Mu opioid receptor
 Protein Family    Mu-type opioid receptor
 UniProt Gene Name    OPRM1 [Similar Products]
 UniProt Synonym Gene Names    MOR1 [Similar Products]
 UniProt Entry Name    OPRM_HUMAN
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 NCBI Summary for OPRM1    The OPRM1 gene encodes the mu opioid receptor, which is the primary site of action for the most commonly used opioids, including morphine, heroin, fentanyl, and methadone. It is also the primary receptor for endogenous opioid peptides beta-endorphin (see POMC, MIM 176830) and the enkephalins (see PENK, MIM 131330). The OPRM1 receptor is a membrane of the G protein-coupled receptor family (Bond et al., 1998 [PubMed 9689128]). There are at least 3 types of opioid receptors, mu, kappa (OPRK1; MIM 165196), and delta (OPRD1; MIM 165195), each with a distinct pharmacologic profile (Chen et al., 1993 [PubMed 8393525]).[supplied by OMIM]
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 UniProt Comments for OPRM1    Function: Inhibits neurotransmitter release by reducing calcium ion currents and increasing potassium ion conductance. Receptor for beta-endorphin.

Subunit structure: Forms a complex with G(alpha)z/i2 subunits and the RGSZ proteins, RGSZ17 and RGSZ20. The formation of this complex results in mu-opioid receptor desensitization. Interacts with RGSZ17 and RGSZ20

By similarity. The C-terminal tail interacts with the C-terminal section of DNAJB4. Ref.17

Subcellular location: Cell membrane; Multi-pass membrane protein.

Polymorphism: Variant Asp-40 does not show altered binding affinities for most opioid peptides and alkaloids tested, but it binds beta-endorphin, an endogenous opioid that activates the mu opioid receptor, approximately 3 times more tightly than the most common allelic form.

Sequence similarities: Belongs to the G-protein coupled receptor 1 family.

Sequence caution: The sequence CAI20458.1 differs from that shown. Reason: Erroneous initiation. The sequence EAW47705.1 differs from that shown. Reason: Erroneous initiation. Isoform 9: The sequence AAQ77392.1 differs from that shown. Reason: a polymorphism leading to premature termination of translation (position: 411:Q->Stop)
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 Research Articles on OPRM1    1. results extend recent preclinical and clinical findings implicating the CRH-BP in stress-related alcoholism and confirm the role of the Asp40 allele of the OPRM1 gene in reward-driven alcohol phenotypes
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-OPRM1 antibodyPathways associated with anti-OPRM1 antibody
 Reference Product  PubMed Publications
 OPRD1 antibody  >17 publications with OPRM1 and OPRD1
 PLD2 antibody  >1 publications with OPRM1 and PLD2
 Products by Pathway  Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 G Alpha (i) Signalling Events Pathway antibodies  G Alpha (i) Signalling Events Pathway Diagram
 G-protein Activation Pathway antibodies  G-protein Activation Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 GPCR Ligand Binding Pathway antibodies  GPCR Ligand Binding Pathway Diagram
 GPCRs, Class A Rhodopsin-like Pathway antibodies  GPCRs, Class A Rhodopsin-like Pathway Diagram
 IL4-mediated Signaling Events Pathway antibodies  IL4-mediated Signaling Events Pathway Diagram
 Neuroactive Ligand-receptor Interaction Pathway antibodies  Neuroactive Ligand-receptor Interaction Pathway Diagram
 Neuroactive Ligand-receptor Interaction Pathway antibodies  Neuroactive Ligand-receptor Interaction Pathway Diagram
 Opioid Signalling Pathway antibodies  Opioid Signalling Pathway Diagram
Diseases associated with anti-OPRM1 antibodyOrgans/Tissues associated with anti-OPRM1 antibody
 Disease Name  Pubmed Publications
 Pain Antibodies  >82 publications with OPRM1 and Pain
 Alcoholism Antibodies  >51 publications with OPRM1 and Alcoholism
 Heroin Dependence Antibodies  >17 publications with OPRM1 and Heroin Dependence
 Neoplasms Antibodies  >7 publications with OPRM1 and Neoplasms
 Brain Diseases Antibodies  >7 publications with OPRM1 and Brain Diseases
 Obesity Antibodies  >5 publications with OPRM1 and Obesity
 Pruritus Antibodies  >4 publications with OPRM1 and Pruritus
 Drug Toxicity Antibodies  >4 publications with OPRM1 and Drug Toxicity
 Epilepsy Antibodies  >4 publications with OPRM1 and Epilepsy
 Skin Diseases Antibodies  >4 publications with OPRM1 and Skin Diseases
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >59 publications with OPRM1 and Brain
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