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anti-ON antibody :: Rabbit anti-Human Osteonectin (ON) Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS2005086 (SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
0.01 mg  /  $110 +1 FREE 8GB USB
0.02 mg  /  $130 +1 FREE 8GB USB
0.05 mg  /  $175 +1 FREE 8GB USB
0.1 mg  /  $225 +1 FREE 8GB USB
0.2 mg  /  $335 +1 FREE 8GB USB
1 mg  /  $790 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Osteonectin (ON), Polyclonal Antibody

★Popular Item★
 Also Known As   

Biotin-Linked Antibody to Osteonectin (ON)

 Product Gene Name   

anti-ON antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Immunogen Sequence    Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGS- APQ QEALPDETEV VEETVAEVTE VSVGANPVQV EVGEFDDGAE ETEEEVVAEN PCQNHHCKHG KVCELDENNT PMCVCQDPTS CPAPIGEFEK VCSNDNKTFD SSCHFFATKC TLEGTKKGHK LHLDYIGPCK YIPPCLDSEL TEFPLRMRDW LKNVLVTLYE RDEDNNLLTE KQKLRVKKIH ENEKRLEAGD HPVELLARDF EKNYNMYIFP VHWQFGQLDQ HPIDGYLSHT ELAPLRAPLI PMEHCTTRFF ETCDLDNDKY IALDEWAGCF GIKQKDIDKD LVI
 Chromosome Location    Chromosome: 5; NC_000005.9 (151040657..151066615, complement). Location: 5q31.3-q32
 OMIM    182120
 3D Structure    ModBase 3D Structure for P09486
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 Clonality    Polyclonal
 Isotype    IgG
 Host    Rabbit
 Species Reactivity    Human
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 Specificity    The antibody is a rabbit polyclonal antibody raised against ON conjugated to biotin. It has beenselected for its ability to recognize ON in immunohistochemical staining andwestern blotting.
 Purity/Purification    Affinity Chromatography
 Form/Format    Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
 Concentration    200ug/ml (lot specific)
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 Immunogen    Osteonectin, Human
 Unconjugated Antibody    The unconjugated antibody version of this item is also available as catalog #MBS2003742
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 Preparation and Storage    Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
 Other Notes    Small volumes of anti-ON antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Applications Tested/Suitable for anti-ON antibody   

Immunocytochemistry (ICC), Immunohistochemistry (IHC), (IHC-f) & (IHC-P), Immunofluorescence (IF), Western Blot (WB)

 Application Notes for anti-ON antibody    Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
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 Western Blot of anti-ON antibody    Sample: Recombinant Osteonectin, Human
anti-ON antibody Western Blot (WB) image
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 SDS-PAGE of anti-ON antibody    [ DESCRIPTION]
Effective Size Range: 10kDa to 70kDa.
Protein bands: 10 kDa, 14 kDa, 18 kDa, 22 kDa, 26 kDa, 33 kDa, 44 kDa and 70 kDa.
Double intensity bands: The 26 kDa, 18 kDa, 10 kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
[ STORAGE BUFFER]
62.5mM Tris-H3P04 (pH 7.5 at 25C), 1 mM EDTA, 2% (w/v) SDS, 100mM DTT, 1 mM NaN3, 0.01 % (w/v) bromo-phenol blue and 33% (v/v) glycerol.
[APPLICATION]
Accurate protein sizing on SDS-polyacrylamide gels and Western blots.
[USAGE]
1. Allow the Marker to reach room temperature and thoroughly mix before use. This will ensure that any solids that may have precipitated at -20C have returned to solution. Do not boil!
2. Load the following volumes of the Marker on a SDS-polyacrylamide gel.
[ LOADING VOLUMES]
3~5uL per well for mini gel; 7uL per well for large gel.
Use the same volumes for Western blotting applications. The loading volumes listed above are recommended for gels with a thickness of 0.75~1.0mm. The loading volume should be double for 1.5mm thick gels.
[ QUALITY CONTROL]
The protein marker, with molecular weight shifts of < or = 5% and minimal band broadening, are confirmed by migration in SDS-PAGE system. Electrophoresis of the Marker on a 13~18% Tris-glycine SDS-polyacrylamide gel resolves 8 individual bands.
[IMPORTANT NOTES]
1. The protein marker is conveniently packaged and ready to use. There is no need to heat, dilute or add reducing agents. Do not boil, which may cause band degradation.
2. The molecular weights of the proteins have a lot-to-Iot variation of ~5%.
3. These protein markers are for SDS-PAGE and should not to be used for native electrophoresis or a native gel. These markers are denatured and have SDS in the storage buffer.
4. Don't load too much protein. See recommended load volumes in the manual.
5. Check % gel that is being used if the lands miss. Depending on gel type and/or percentage, you may not see all of the bands. For example, one would not see the smallest bands of the standard on a very low % gel. A high % gel may not resolve the higher MW bands. Homogeneous low percentage gels are recommended for analysis of large proteins and high percentage gels for analysis of small proteins. In high percentage gels (13~18%) large proteins (22~70kDa) can separate, while in low percentage gels (4~8%) small proteins (14 and 10kDa) will migrate with the tracking dye. Longer transfer times or higher transfer voltages may be required for Western blotting of large (>100kDa) proteins.
6. If additional bands are observed in the gel image of the protein ladder, this might be caused by Dn oxidation in the storage buffer.
7. If proteins show a poor transfer, increase voltage, current or length of time for transfer, and pay attention to the SDS from the transfer buffer. SDS will cause the proteins to bind less efficiently to membranes because it disrupts the hydrophobic interaction between the membrane and the protein. If SDS is present in transfer buffer, make sure that there is no more than 0.02% in buffer.
8. Alcohol enhances hydrophobic binding to membrane. Not enough alcohol may prevent binding.
[ SHELF LIFE]
Storage: -20 degree C, 1 year at -20 degree C. If the product has been stored as directed for one year, its shelf life maybe extended by adding dithiothreitol (Dn) to approximately 50mM.
anti-ON antibody SDS-PAGE image
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NCBI/Uniprot data below describe general gene information for ON. It may not necessarily be applicable to this product.
 NCBI GI #    338325
 NCBI GeneID    6678
 NCBI Accession #    AAA60993.1 [Other Products]
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 UniProt Primary Accession #    P09486 [Other Products]
 UniProt Secondary Accession #    Q6IBK4; D3DQH9 [Other Products]
 UniProt Related Accession #    P09486 [Other Products]
 Molecular Weight    34,632 Da
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 NCBI Official Full Name    osteonectin
 NCBI Official Synonym Full Names    secreted protein, acidic, cysteine-rich (osteonectin)
 NCBI Official Symbol    SPARC [Similar Products]
 NCBI Official Synonym Symbols   
ON
[Similar Products]
 NCBI Protein Information    SPARC; BM-40; osteonectin; cysteine-rich protein; basement-membrane protein 40; secreted protein acidic and rich in cysteine
 UniProt Protein Name    SPARC
 UniProt Synonym Protein Names   
Basement-membrane protein 40; BM-40; Osteonectin; ON; Secreted protein acidic and rich in cysteine
 UniProt Gene Name    SPARC [Similar Products]
 UniProt Synonym Gene Names    ON; BM-40; ON [Similar Products]
 UniProt Entry Name    SPRC_HUMAN
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 NCBI Summary for ON    This gene encodes a cysteine-rich acidic matrix-associated protein. The encoded protein is required for the collagen in bone to become calcified but is also involved in extracellular matrix synthesis and promotion of changes to cell shape. The gene product has been associated with tumor suppression but has also been correlated with metastasis based on changes to cell shape which can promote tumor cell invasion. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2015]
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 UniProt Comments for ON    SPARC: Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity. Belongs to the SPARC family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 5q31.3-q32

Cellular Component: extracellular space; platelet alpha granule membrane; cell surface; nuclear matrix; mitochondrion; cytoplasm; extracellular region; plasma membrane; basement membrane

Molecular Function: collagen binding; protein binding; extracellular matrix binding; calcium ion binding

Biological Process: response to gravity; platelet activation; receptor-mediated endocytosis; response to peptide hormone stimulus; ossification; extracellular matrix organization and biogenesis; response to cAMP; heart development; response to glucocorticoid stimulus; response to lipopolysaccharide; signal transduction; response to L-ascorbic acid; negative regulation of angiogenesis; platelet degranulation; response to cadmium ion; response to ethanol; response to cytokine stimulus; negative regulation of endothelial cell proliferation; response to lead ion; regulation of cell morphogenesis; blood coagulation; response to calcium ion; inner ear development; lung development

Disease: Osteogenesis Imperfecta, Type Xvii
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 Research Articles on ON    1. role of SPARC, MMP-2 and MMP-9 in intracranial aneurysms
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-ON antibodyPathways associated with anti-ON antibody
 Reference Product  PubMed Publications
 TGFB1 antibody  >64 publications with ON and TGFB1
 COL1A1 antibody  >24 publications with ON and COL1A1
 EGF antibody  >20 publications with ON and EGF
 SERPINE1 antibody  >16 publications with ON and SERPINE1
 FN1 antibody  >11 publications with ON and FN1
 TIMP1 antibody  >4 publications with ON and TIMP1
 PLG antibody  >1 publications with ON and PLG
 Products by Pathway  Pathway Diagram
 Binding And Uptake Of Ligands By Scavenger Receptors Pathway antibodies  Binding And Uptake Of Ligands By Scavenger Receptors Pathway Diagram
 ECM Proteoglycans Pathway antibodies  ECM Proteoglycans Pathway Diagram
 Extracellular Matrix Organization Pathway antibodies  Extracellular Matrix Organization Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
 Platelet Activation, Signaling And Aggregation Pathway antibodies  Platelet Activation, Signaling And Aggregation Pathway Diagram
 Platelet Degranulation Pathway antibodies  Platelet Degranulation Pathway Diagram
 Response To Elevated Platelet Cytosolic Ca2+ Pathway antibodies  Response To Elevated Platelet Cytosolic Ca2+ Pathway Diagram
 Scavenging By Class H Receptors Pathway antibodies  Scavenging By Class H Receptors Pathway Diagram
 Senescence And Autophagy Pathway antibodies  Senescence And Autophagy Pathway Diagram
 TGF-beta Receptor Signaling Pathway antibodies  TGF-beta Receptor Signaling Pathway Diagram
Diseases associated with anti-ON antibodyOrgans/Tissues associated with anti-ON antibody
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >554 publications with ON and Neoplasms
 Cardiovascular Diseases Antibodies  >101 publications with ON and Cardiovascular Diseases
 Adenocarcinoma Antibodies  >98 publications with ON and Adenocarcinoma
 Inflammation Antibodies  >94 publications with ON and Inflammation
 Fibrosis Antibodies  >86 publications with ON and Fibrosis
 Brain Diseases Antibodies  >71 publications with ON and Brain Diseases
 Breast Neoplasms Antibodies  >59 publications with ON and Breast Neoplasms
 Necrosis Antibodies  >58 publications with ON and Necrosis
 Heart Diseases Antibodies  >54 publications with ON and Heart Diseases
 Neoplasms, Experimental Antibodies  >44 publications with ON and Neoplasms, Experimental
 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >1011 publications with ON and Bone
 Connective Tissue Antibodies  >424 publications with ON and Connective Tissue
 Blood Antibodies  >307 publications with ON and Blood
 Vascular Antibodies  >276 publications with ON and Vascular
 Bone Marrow Antibodies  >184 publications with ON and Bone Marrow
 Muscle Antibodies  >167 publications with ON and Muscle
 Embryonic Tissue Antibodies  >167 publications with ON and Embryonic Tissue
 Mouth Antibodies  >147 publications with ON and Mouth
 Brain Antibodies  >138 publications with ON and Brain
 Skin Antibodies  >113 publications with ON and Skin
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