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anti-SPP1 antibody :: Goat Osteopontin/SPP1 Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS421341
Western Blot (WB)
Unit / Price
0.1 mg  /  $300 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Osteopontin/SPP1, Polyclonal Antibody

 Also Known As   

Goat anti-Osteopontin/SPP1 Antibody

 Product Synonym Names    SPP1; HGNC: 11255; secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1); BNSP; BSPI; ETA-1; MGC110940; OPN; Secreted phosphoprotein-1 (osteopontin, bone sialoprotein); secreted phosphoprotein 1; SPP1/CALPHA1 fusion; SPP1 antibody; HGNC: 11255 antibody; secreted phosphoprotein 1 (osteopontin; bone sialoprotein I; early T-lymphocyte activation 1) antibody; BNSP antibody; BSPI antibody; ETA-1 antibody; MGC110940 antibody; OPN antibody; Secreted phosphoprotein-1 (osteopontin; bone sialoprotein) antibody; secreted phosphoprotein 1 antibody; SPP1/CALPHA1 fusion antibody; bone sialoprotein I antibody; early T-lymphocyte activation 1 antibody; osteopontin antibody; osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein antibody; Osteopontin; Osteopontin / SPP1
 Product Gene Name   

anti-SPP1 antibody

[Similar Products]
 Antibody/Peptide Pairs    Osteopontin / SPP1 peptide (MBS425969) is used for blocking the activity of Osteopontin/SPP1 antibody (MBS421341)
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Immunogen Sequence    DPKSKEEDKH LK
 OMIM    166490
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 Clonality    Polyclonal
 Host    Goat
 Species Reactivity    Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog
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 Purity/Purification    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
 Form/Format    Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
 Concentration    100ug specific antibody in 200ul (lot specific)
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 Immunogen    Peptide with sequence C-DPKSKEEDKHLK, from the C Terminus of the protein sequence according to NP_001035147.1; NP_000573.1; NP_001035149.1.
 Epitope    C Terminus
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 Preparation and Storage    Aliquot and store at -20 degree C. Minimize freezing and thawing.
 ISO Certification    Manufactured in an ISO 9001:2015 Certified Laboratory.
 Other Notes    Small volumes of anti-SPP1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Applications Tested/Suitable for anti-SPP1 antibody   

Peptide ELISA (EIA), Western Blot (WB)

 Application Notes for anti-SPP1 antibody    Peptide ELISA: Antibody detection limit dilution 1: 32000.
Western Blot: Approx 60kDa band observed in lysates of Human Tonsil and Spleen and lysates of cell line MOLT4 and Jurkat (calculated MW of 33.8kDa according to NP_000573.1). The observed molecular weight corresponds to earlier findings in literature (Singh et al, J Biol Chem.1995 Nov 24;270(47): 28471-8; PMID: 7499354). Recommended concentration: 0.5-1.5ug/ml.
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 Western Blot (WB) of anti-SPP1 antibody    (0.5ug/ml) staining of MOLT4 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
anti-SPP1 antibody Western Blot (WB) (WB) image
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NCBI/Uniprot data below describe general gene information for SPP1. It may not necessarily be applicable to this product.
 NCBI GI #    91206462
 NCBI GeneID    6696
 NCBI Accession #    NP_001035147.1 [Other Products]
 NCBI Related Accession #    NP_000573.1; NP_001035149.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001040058.1 [Other Products]
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 UniProt Secondary Accession #    Q15681; Q15682; Q15683; Q4W597; Q567T5; Q8NBK2; Q96IZ1; B2RDA1 [Other Products]
 UniProt Related Accession #    P10451 [Other Products]
 Molecular Weight    33,843 Da
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 NCBI Official Full Name    osteopontin isoform OPN-a
 NCBI Official Synonym Full Names    secreted phosphoprotein 1
 NCBI Official Symbol    SPP1 [Similar Products]
 NCBI Official Synonym Symbols   
OPN; BNSP; BSPI; ETA-1
[Similar Products]
 NCBI Protein Information    osteopontin
 UniProt Protein Name    Osteopontin
 UniProt Synonym Protein Names   
Bone sialoprotein 1; Nephropontin; Secreted phosphoprotein 1; SPP-1; Urinary stone protein; Uropontin
 Protein Family    Osteopontin
 UniProt Gene Name    SPP1 [Similar Products]
 UniProt Synonym Gene Names    BNSP; OPN; SPP-1 [Similar Products]
 UniProt Entry Name    OSTP_HUMAN
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 NCBI Summary for SPP1    The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]
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 UniProt Comments for SPP1    osteopontin: Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Belongs to the osteopontin family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 4q22.1

Cellular Component: cell projection; extracellular region; extracellular space; perinuclear region of cytoplasm

Molecular Function: cytokine activity; extracellular matrix binding; protein binding

Biological Process: biomineral formation; cell adhesion; decidualization; embryo implantation; extracellular matrix disassembly; extracellular matrix organization and biogenesis; inflammatory response; negative regulation of collateral sprouting of intact axon in response to injury; osteoblast differentiation; positive regulation of bone resorption; response to steroid hormone stimulus; response to vitamin D
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 Research Articles on SPP1    1. Results show high matrix stiffness upregulated OPN expression in hepatocellular carcinoma (HCC) via the integrin beta1/GSK-3beta/beta-catenin signaling pathway and highlight the physicomechanical and biochemical signals contributing to OPN expression in HCC cells.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-SPP1 antibodyPathways associated with anti-SPP1 antibody
 Reference Product  PubMed Publications
 RUNX2 antibody  >161 publications with SPP1 and RUNX2
 CD44 antibody  >136 publications with SPP1 and CD44
 BMP2 antibody  >80 publications with SPP1 and BMP2
 THBS1 antibody  >14 publications with SPP1 and THBS1
 ACP5 antibody  >12 publications with SPP1 and ACP5
 MMP7 antibody  >11 publications with SPP1 and MMP7
 TP53 antibody  >4 publications with SPP1 and TP53
 ITGB1 antibody  >3 publications with SPP1 and ITGB1
 ITGAV antibody  >3 publications with SPP1 and ITGAV
 Products by Pathway  Pathway Diagram
 BDNF Signaling Pathway antibodies  BDNF Signaling Pathway Diagram
 Degradation Of The Extracellular Matrix Pathway antibodies  Degradation Of The Extracellular Matrix Pathway Diagram
 Direct P53 Effectors Pathway antibodies  Direct P53 Effectors Pathway Diagram
 ECM-receptor Interaction Pathway antibodies  ECM-receptor Interaction Pathway Diagram
 ECM-receptor Interaction Pathway antibodies  ECM-receptor Interaction Pathway Diagram
 Endochondral Ossification Pathway antibodies  Endochondral Ossification Pathway Diagram
 Extracellular Matrix Organization Pathway antibodies  Extracellular Matrix Organization Pathway Diagram
 FGF Signaling Pathway antibodies  FGF Signaling Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
Diseases associated with anti-SPP1 antibodyOrgans/Tissues associated with anti-SPP1 antibody
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >268 publications with SPP1 and Inflammation
 Kidney Diseases Antibodies  >235 publications with SPP1 and Kidney Diseases
 Fibrosis Antibodies  >166 publications with SPP1 and Fibrosis
 Neoplasm Metastasis Antibodies  >140 publications with SPP1 and Neoplasm Metastasis
 Necrosis Antibodies  >132 publications with SPP1 and Necrosis
 Calcinosis Antibodies  >117 publications with SPP1 and Calcinosis
 Adenocarcinoma Antibodies  >113 publications with SPP1 and Adenocarcinoma
 Heart Diseases Antibodies  >109 publications with SPP1 and Heart Diseases
 Neoplasms, Experimental Antibodies  >85 publications with SPP1 and Neoplasms, Experimental
 Breast Neoplasms Antibodies  >79 publications with SPP1 and Breast Neoplasms
 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >1114 publications with SPP1 and Bone
 Blood Antibodies  >567 publications with SPP1 and Blood
 Kidney Antibodies  >371 publications with SPP1 and Kidney
 Vascular Antibodies  >359 publications with SPP1 and Vascular
 Connective Tissue Antibodies  >346 publications with SPP1 and Connective Tissue
 Muscle Antibodies  >296 publications with SPP1 and Muscle
 Bone Marrow Antibodies  >200 publications with SPP1 and Bone Marrow
 Mouth Antibodies  >189 publications with SPP1 and Mouth
 Heart Antibodies  >139 publications with SPP1 and Heart
 Liver Antibodies  >138 publications with SPP1 and Liver
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