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anti-PSME1 antibody :: Rabbit PA28, alpha Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS611019 anti-PSME1 antibody
Unit / Price
0.1 mg  /  $620 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

PA28, alpha (PSME1), Polyclonal Antibody

 Also Known As   

PA28, alpha, CT (Proteasome Activator 28-alpha subunit)

 Product Synonym Names    Anti -PA28, alpha, CT (Proteasome Activator 28-alpha subunit)
 Product Gene Name   

anti-PSME1 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Chromosome Location    Chromosome: 14; NC_000014.8 (24605378..24608176). Location: 14q11.2
 OMIM    600654
 3D Structure    ModBase 3D Structure for Q06323
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 Clonality    Polyclonal
 Isotype    IgG
 Host    Rabbit
 Species Reactivity    Human, Macaque, Mouse, Porcine, Rat
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 Specificity    Reacts with the C-terminal region of mouse, rat, human, crab-eating macaque (monkey) and pig PA28a. On Western blots, it identifies the target band at ~28kD. Faint bands of unknown origin may be observed in some homogenate or lysate preparations.
 Purity/Purification    Affinity Purified
Epitope-affinity purified from rabbit antiserum.
 Form/Format    Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide, before the addition of glycerol to 40%.
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 Immunogen    Synthetic peptide derived from the C-terminal region of the proteasome activator 28-alpha subunit (PA28a, also known as 11s regulator complex alpha subunit (REG-alpha) (11S REG) and Interferon gamma up-regulated I-5111 protein (IGUP I-5111) protein.
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 Preparation and Storage    May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
 Other Notes    Small volumes of anti-PSME1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-PSME1 antibody

   PA281 is a 180kD proteasome activator protein that is highly conserved among species.2,3 The PA28 complex is composed of two homologous subunits,a and b, which have similar catalytic properties and associate to form a hexameric ring4-5 that b binds to both ends of the 20S proteasome.6 PA28 is a strong proteasome activator; its affinity for the proteasome is about 10-fold greater than recombinant PA28b.5 The carboxy terminus of PA28a is necessary for PA28 to bind to and activate the proteasome.7 The activation of the proteasome by PA28 is an interferon-g (IFNg) inducible.8 PA28 is constitutively expressed by antigen presenting cells and is required for presentation of certain major histocompatibility (MHC) class I antigens.9-10 PA28 a/b can enhance antigen processing independently of changes in 20S proteasome subunit composition or assembly.11 Down-regulation of PA28 results in abnormal proteasome activation, and has been implicated in the development of intimal hyperplasia (IH) in animal models.6 Detection of anti-PA28a antibody is significantly higher in serum from patients with systemic lupus erythematosus (SLE) and Sjogren's syndrome (SS), suggesting the importance of an antigen-driven system in the induction of an autoimmune response to PA28 complex.12 Impaired expression of proteasome subunits is involved in the loss of HLA class I expression in human colon cancer cells.13
 Product Categories/Family for anti-PSME1 antibody    Antibodies; Abs to Proteasomes
 Applications Tested/Suitable for anti-PSME1 antibody   

Western Blot (WB)

 Application Notes for anti-PSME1 antibody    Suitable for use in Western Blot.
Dilution: Western Blot: 1-3ug/ml
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NCBI/Uniprot data below describe general gene information for PSME1. It may not necessarily be applicable to this product.
 NCBI GI #    3219284
 NCBI GeneID    5720
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 UniProt Primary Accession #    Q06323 [Other Products]
 UniProt Secondary Accession #    Q9UEF4 [Other Products]
 UniProt Related Accession #    Q06323; Q6FHU3; Q6IBM2; Q86SZ9 [Other Products]
 Molecular Weight    28,723 Da [Similar Products]
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 NCBI Official Full Name    PA28 alpha subunit
 NCBI Official Synonym Full Names    proteasome (prosome, macropain) activator subunit 1 (PA28 alpha)
 NCBI Official Symbol    PSME1 [Similar Products]
 NCBI Official Synonym Symbols   
PA28A; IFI5111; MGC8628; REGalpha; PA28alpha
[Similar Products]
 NCBI Protein Information    proteasome activator complex subunit 1; REG-alpha; IGUP I-5111; OTTHUMP00000027975; OTTHUMP00000164702; 29-kD MCP activator subunit; interferon-gamma IEF SSP 5111; proteasome activator subunit-1; 11S regulator complex alpha subunit; 11S regulator complex subunit alpha; proteasome activator 28 subunit alpha; interferon-gamma-inducible protein 5111; interferon gamma up-regulated I-5111 protein; activator of multicatalytic protease subunit 1
 UniProt Protein Name    Proteasome activator complex subunit 1
 UniProt Synonym Protein Names   
11S regulator complex subunit alpha; REG-alpha; Activator of multicatalytic protease subunit 1; Interferon gamma up-regulated I-5111 protein; IGUP I-5111; Proteasome activator 28 subunit alpha
 Protein Family    Proteasome activator complex
 UniProt Gene Name    PSME1 [Similar Products]
 UniProt Synonym Gene Names    IFI5111 [Similar Products]
 UniProt Entry Name    PSME1_HUMAN
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 NCBI Summary for PSME1    The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The immunoproteasome contains an alternate regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator. Three subunits (alpha, beta and gamma) of the 11S regulator have been identified. This gene encodes the alpha subunit of the 11S regulator, one of the two 11S subunits that is induced by gamma-interferon. Three alpha and three beta subunits combine to form a heterohexameric ring. Two transcripts encoding different isoforms have been identified. [provided by RefSeq]
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 UniProt Comments for PSME1    PSME1: Implicated in immunoproteasome assembly and required for efficient antigen processing. The PA28 activator complex enhances the generation of class I binding peptides by altering the cleavage pattern of the proteasome. Heterodimer of PSME1 and PSME2, which forms a hexadimeric ring. PSME1 can form homoheptamers. By IFNG/IFN-gamma. Belongs to the PA28 family.

Protein type: Proteasome complex

Chromosomal Location of Human Ortholog: 14q11.2

Cellular Component: proteasome complex; nucleoplasm; proteasome activator complex; cytoplasm; cytosol

Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; protein polyubiquitination; viral reproduction; apoptosis; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; regulation of apoptosis; antigen processing and presentation of peptide antigen via MHC class I; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I; gene expression; mitotic cell cycle; regulation of amino acid metabolic process; negative regulation of apoptosis; G1/S transition of mitotic cell cycle
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 Research Articles on PSME1    1. bortezomib-adapted HL-60 cells showed increased expression and proteasome association of the 11S proteasome activator
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-PSME1 antibodyPathways associated with anti-PSME1 antibody
 Reference Product  PubMed Publications
 PSME2 antibody  >47 publications with PSME1 and PSME2
 Products by Pathway  Pathway Diagram
 APC/C-mediated Degradation Of Cell Cycle Proteins Pathway antibodies  APC/C-mediated Degradation Of Cell Cycle Proteins Pathway Diagram
 APC/C:Cdc20 Mediated Degradation Of Securin Pathway antibodies  APC/C:Cdc20 Mediated Degradation Of Securin Pathway Diagram
 APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway antibodies  APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway Diagram
 APC/C:Cdh1 Mediated Degradation Of Cdc20 And Other APC/C:Cdh1 Targeted Proteins In Late Mitosis/early G1 Pathway antibodies  APC/C:Cdh1 Mediated Degradation Of Cdc20 And Other APC/C:Cdh1 Targeted Proteins In Late Mitosis/early G1 Pathway Diagram
 Activation Of APC/C And APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway antibodies  Activation Of APC/C And APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway Diagram
 Adaptive Immunity Signaling Pathway antibodies  Adaptive Immunity Signaling Pathway Diagram
 Antigen Processing And Presentation Pathway antibodies  Antigen Processing And Presentation Pathway Diagram
 Antigen Processing And Presentation Pathway antibodies  Antigen Processing And Presentation Pathway Diagram
 Antigen Processing: Ubiquitination & Proteasome Degradation Pathway antibodies  Antigen Processing: Ubiquitination & Proteasome Degradation Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
Diseases associated with anti-PSME1 antibodyOrgans/Tissues associated with anti-PSME1 antibody
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >17 publications with PSME1 and Neoplasms
 Carcinoma Antibodies  >9 publications with PSME1 and Carcinoma
 Liver Diseases Antibodies  >6 publications with PSME1 and Liver Diseases
 Inflammation Antibodies  >5 publications with PSME1 and Inflammation
 Adenocarcinoma Antibodies  >5 publications with PSME1 and Adenocarcinoma
 Death Antibodies  >4 publications with PSME1 and Death
 Necrosis Antibodies  >4 publications with PSME1 and Necrosis
 Carcinoma, Squamous Cell Antibodies  >3 publications with PSME1 and Carcinoma, Squamous Cell
 Carcinoma, Hepatocellular Antibodies  >3 publications with PSME1 and Carcinoma, Hepatocellular
 Liver Neoplasms Antibodies  >3 publications with PSME1 and Liver Neoplasms
 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >97 publications with PSME1 and Muscle
 Blood Antibodies  >38 publications with PSME1 and Blood
 Liver Antibodies  >10 publications with PSME1 and Liver
 Brain Antibodies  >8 publications with PSME1 and Brain
 Skin Antibodies  >4 publications with PSME1 and Skin
 Spleen Antibodies  >4 publications with PSME1 and Spleen
 Bone Marrow Antibodies  >3 publications with PSME1 and Bone Marrow
 Nerve Antibodies  >3 publications with PSME1 and Nerve
 Bone Antibodies  >3 publications with PSME1 and Bone
 Vascular Antibodies  >3 publications with PSME1 and Vascular
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