View our list of available Coronavirus (COVID-19, SARS-CoV-2, 2019-nCOV) antibodies, recombinant proteins, ELISA Kits... Options Full Datasheet Printer Friendly Datasheet Prepare for Printing Full Datasheet Printer Friendly Datasheet Prepare for Printing Have Questions? Ask Us! Request A Quote Today! Add To Compare List Rabbit anti-Human PAK1,2,3 Polyclonal Antibody Catalog # MBS622951 Unit / Price 10 Blots / $820 +1 FREE 8GB USB 5x10 Blots / $3,520 +4 FREE 8GB USB Product Name Product Info Accession #s Product Desc Diseases/Tissues/Pathways Applications References Product Name PAK1,2,3, Polyclonal Antibody Full Product Name PAK1,2,3, phosphorylated (Thr423/Thr402/Thr421) (p21 Activated Kinase 1,2,3) Product Synonym Names Anti -PAK1,2,3, phosphorylated (Thr423/Thr402/Thr421) (p21 Activated Kinase 1,2,3) Research Use Only For Research Use Only. Not for use in diagnostic procedures. TOP Clonality Polyclonal Host Rabbit Species Reactivity Human Specificity Recognizes human PAK1, PAK2, and PAK3 when phosphorylated at phosphorylated Thr423/Thr402/Thr421, respectively. Other species (100% homologous) have not been tested, but are expected to react. Purity/Purification Affinity PurifiedPurified by immunoaffinity chromatography. Form/Format Supplied as a liquid in Dulbecco's PBS (without Mg2+ and Ca2+), 1mg/ml BSA (IgG, protease free), pH 7.2, 0.05% sodium azide, 50% glycerol. Immunogen Synthetic phosphopeptide derived from a region of human PAK that contains threonine 423 (for PAK1). The sequence is conserved across many species for all three main isoforms of PAK. Positive Control His-tagged wild-type and S139A/T421A double mutant PAK3. Preparation and Storage May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Other Notes Small volumes of anti-PAK1,2,3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply. TOP Related Product Information for anti-PAK1,2,3 antibody p21-activated kinase (PAK) is actually a family of serine/threonine protein kinases, members of which are activated by small molecular weight GTPases. The three most common isoforms are PAK1, PAK2 and PAK3 (also known as a-PAK, g-PAK andb -PAK, respectively). These kinases contain numerous regulatory elements that trigger diverse signaling processes such as those initiated by activated GTPases, interaction with Src homology 3 (SH3) domains and caspase mediated proteolytic cleavage. Autophosphorylation of threonine 423 (threonine 402 for PAK2 and threonine 421 for PAK3), catalyzed by Cdc42, is required for activation of PAK. Product Categories/Family for anti-PAK1,2,3 antibody Antibodies; Abs to Protein Kinases Applications Tested/Suitable for anti-PAK1,2,3 antibody ELISA (EL/EIA), Western Blot (WB) Application Notes for anti-PAK1,2,3 antibody Suitable for use in Western Blot.Dilution: Western Blot: 1:1000 SDS-Page of anti-PAK1,2,3 antibody Extracts prepared from his-tagged S139A/T421A double mutant (1) and wild-type PAK3 (2-5) were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4oC, then were incubated with MBS622951for two hours at RT in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1, 2), the non phosphopeptide corresponding to the immunogen (3), a generic phosphothreonine containing peptide (4), or, the phosphopeptide immunogen (5). After washing, membranes were incubated with goat F(ab’)2 antirabbit IgG alkaline phosphatase and signals were detected using the Tropix WesternStar method. The data show that only the peptide corresponding to PAK1/2/3 [pT423] blocks the antibody signal, and phosphorylation with the wild-type but not the mutant protein, thereby demonstrating the specificity of the antibody. TOP Precautions All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines. Disclaimer While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information. TOP