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anti-PCK1 antibody :: Goat PCK1/PEPCKC Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS420472
Western Blot (WB)
Unit / Price
0.1 mg  /  $300 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

PCK1/PEPCKC, Polyclonal Antibody

 Also Known As   

Goat anti-PCK1/PEPCKC (internal region) Antibody

 Product Synonym Names    Goat Anti-PCK1 / PEPCKC (internal) Antibody; PCK1; PEPCKC; phosphoenolpyruvate carboxykinase 1 (soluble); MGC22652; PEPCK1; PEP carboxykinase; cytosolic phosphoenolpyruvate carboxykinase 1; phosphoenolpyruvate carboxylase; phosphopyruvate carboxylase; PCK1 antibody; PEPCKC antibody; phosphoenolpyruvate carboxykinase 1 (soluble) antibody; MGC22652 antibody; PEPCK1 antibody; PEP carboxykinase antibody; cytosolic phosphoenolpyruvate carboxykinase 1 antibody; phosphoenolpyruvate carboxylase antibody; phosphopyruvate carboxylase antibody; PCK1 / PEPCKC (internal region)
 Product Gene Name   

anti-PCK1 antibody

[Similar Products]
 Product Synonym Gene Name    PEPCKC [Similar Products]
 Antibody/Peptide Pairs    PCK1 / PEPCKC peptide (MBS425702) is used for blocking the activity of PCK1/PEPCKC antibody (MBS420472)
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Immunogen Sequence    EKEVEDIEKY LEDQ
 OMIM    261680
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 Clonality    Polyclonal
 Host    Goat
 Species Reactivity    Tested: Human; Expected from sequence similarity: Human
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 Purity/Purification    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
 Form/Format    Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
 Concentration    100ug specific antibody in 200ul (lot specific)
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 Immunogen    Peptide with sequence C-EKEVEDIEKYLEDQ, from the internal region (near the C Terminus) of the protein sequence according to NP_002582.2.
 Epitope    Internal region (near the C Terminus)
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 Preparation and Storage    Aliquot and store at -20 degree C. Minimize freezing and thawing.
 ISO Certification    Manufactured in an ISO 9001:2015 Certified Laboratory.
 Other Notes    Small volumes of anti-PCK1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Applications Tested/Suitable for anti-PCK1 antibody   

Peptide ELISA (EIA), Western Blot (WB)

 Application Notes for anti-PCK1 antibody    Peptide ELISA: Antibody detection limit dilution 1: 16000.
Western Blot: Approx 70kDa band observed in Human Kidney lysates (calculated MW of 69.2kDa according to NP_002582.2). Recommended concentration: 0.5-1.5ug/ml.
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 Western Blot (WB) of anti-PCK1 antibody    (0.5ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
anti-PCK1 antibody Western Blot (WB) (WB) image
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NCBI/Uniprot data below describe general gene information for PCK1. It may not necessarily be applicable to this product.
 NCBI GI #    32483401
 NCBI GeneID    5105
 NCBI Accession #    NP_002582.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_002591.2 [Other Products]
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 UniProt Secondary Accession #    Q8TCA3; Q9UJD2; A8K437; B4DT64 [Other Products]
 UniProt Related Accession #    P35558 [Other Products]
 Molecular Weight    34,156 Da
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 NCBI Official Full Name    cytosolic phosphoenolpyruvate carboxykinase 1
 NCBI Official Synonym Full Names    phosphoenolpyruvate carboxykinase 1
 NCBI Official Symbol    PCK1 [Similar Products]
 NCBI Official Synonym Symbols   
PEPCK1; PEPCKC; PEPCK-C
[Similar Products]
 NCBI Protein Information    phosphoenolpyruvate carboxykinase, cytosolic [GTP]
 UniProt Protein Name    Phosphoenolpyruvate carboxykinase, cytosolic [GTP]
 Protein Family    Protein kinase
 UniProt Gene Name    PCK1 [Similar Products]
 UniProt Synonym Gene Names    PEPCK-C [Similar Products]
 UniProt Entry Name    PCKGC_HUMAN
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 NCBI Summary for PCK1    This gene is a main control point for the regulation of gluconeogenesis. The cytosolic enzyme encoded by this gene, along with GTP, catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. The expression of this gene can be regulated by insulin, glucocorticoids, glucagon, cAMP, and diet. Defects in this gene are a cause of cytosolic phosphoenolpyruvate carboxykinase deficiency. A mitochondrial isozyme of the encoded protein also has been characterized. [provided by RefSeq, Jul 2008]
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 UniProt Comments for PCK1    PCK1: Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle. Defects in PCK1 are the cause of cytosolic phosphoenolpyruvate carboxykinase deficiency (C-PEPCKD). A metabolic disorder resulting from impaired gluconeogenesis. It is a rare disease with less than 10 cases reported in the literature. Clinical characteristics include hypotonia, hepatomegaly, failure to thrive, lactic acidosis and hypoglycemia. Autoposy reveals fatty infiltration of both the liver and kidneys. The disorder is transmitted as an autosomal recessive trait. Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.

Protein type: Carbohydrate Metabolism - glycolysis and gluconeogenesis; Lyase; Carbohydrate Metabolism - pyruvate; Kinase, other; Carbohydrate Metabolism - citrate (TCA) cycle; EC 4.1.1.32

Chromosomal Location of Human Ortholog: 20q13.31

Cellular Component: cytoplasm; cytosol

Molecular Function: carboxylic acid binding; GDP binding; GTP binding; magnesium ion binding; manganese ion binding; phosphoenolpyruvate carboxykinase (GTP) activity

Biological Process: cellular response to potassium ion starvation; gluconeogenesis; glucose homeostasis; glucose metabolic process; glycerol biosynthetic process from pyruvate; internal protein amino acid acetylation; oxaloacetate metabolic process; response to activity; response to insulin stimulus

Disease: Phosphoenolpyruvate Carboxykinase Deficiency, Cytosolic
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 Research Articles on PCK1    1. A mutation of PCK1 associated with Smith-Magenis Syndrome, cytosolic PEPCK deficiency and NMDA receptor glutamate insensitivity.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with anti-PCK1 antibodyDiseases associated with anti-PCK1 antibody
 Products by Pathway  Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 Abacavir Metabolism Pathway antibodies  Abacavir Metabolism Pathway Diagram
 Abacavir Transport And Metabolism Pathway antibodies  Abacavir Transport And Metabolism Pathway Diagram
 Adipocytokine Signaling Pathway antibodies  Adipocytokine Signaling Pathway Diagram
 Adipocytokine Signaling Pathway antibodies  Adipocytokine Signaling Pathway Diagram
 Adipogenesis Pathway antibodies  Adipogenesis Pathway Diagram
 Citrate Cycle (TCA Cycle) Pathway antibodies  Citrate Cycle (TCA Cycle) Pathway Diagram
 Citrate Cycle (TCA Cycle) Pathway antibodies  Citrate Cycle (TCA Cycle) Pathway Diagram
 Developmental Biology Pathway antibodies  Developmental Biology Pathway Diagram
 Disease Name  Pubmed Publications
 Insulin Resistance Antibodies  >36 publications with PCK1 and Insulin Resistance
 Fatty Liver Antibodies  >35 publications with PCK1 and Fatty Liver
 Diabetes Mellitus Antibodies  >34 publications with PCK1 and Diabetes Mellitus
 Liver Diseases Antibodies  >26 publications with PCK1 and Liver Diseases
 Obesity Antibodies  >24 publications with PCK1 and Obesity
 Hyperglycemia Antibodies  >20 publications with PCK1 and Hyperglycemia
 Inflammation Antibodies  >15 publications with PCK1 and Inflammation
 Disease Models, Animal Antibodies  >13 publications with PCK1 and Disease Models, Animal
 Weight Gain Antibodies  >8 publications with PCK1 and Weight Gain
 Glucose Intolerance Antibodies  >8 publications with PCK1 and Glucose Intolerance
Organs/Tissues associated with anti-PCK1 antibody
 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >101 publications with PCK1 and Liver
 Blood Antibodies  >57 publications with PCK1 and Blood
 Adipose Tissue Antibodies  >33 publications with PCK1 and Adipose Tissue
 Muscle Antibodies  >13 publications with PCK1 and Muscle
 Kidney Antibodies  >10 publications with PCK1 and Kidney
 Brain Antibodies  >9 publications with PCK1 and Brain
 Pancreas Antibodies  >6 publications with PCK1 and Pancreas
 Intestine Antibodies  >6 publications with PCK1 and Intestine
 Ovary Antibodies  >6 publications with PCK1 and Ovary
 Heart Antibodies  >4 publications with PCK1 and Heart
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