Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource rightarrow Antibody rightarrow PCNA rightarrow Polyclonal PCNA  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Menu separator
separator
ELISA Kit ELISA Kit
CLIA Kit CLIA Kit
PCR Kit PCR Kit
Monoclonal Antibody Monoclonal Antibody
Polyclonal Antibody Polyclonal Antibody
Secondary Antibody Secondary Antibody
Antigen Antigen
Biochemical Biochemical
cDNA Clone cDNA Clone
siRNA siRNA
Peptide Peptide
Recombinant/Purified Protein Rec./Purified Protein

Custom ELISA Kit Custom ELISA Kit
Custom Protein Custom Protein
Custom Antibody Custom Antibody
Antibody Matched Pairs Antibody Matched Pairs
Antibody & Corresponding Blocking Peptide Pairs Antibody Peptide Pairs
Phospho-Specific Antibodies Phospho Antibodies
Products by Disease Products by Disease
Products by Pathway Products by Pathway
Products by Tissue Products by Tissue

arrow Advanced Search
arrow Submit Technical Q&A
arrow International Distributors
arrow Contact Us
separator
Our Best Sellers moreseparator
separator
 • Human Chorionic Gonadotropin (hCG) alpha Antibody
 • Sialidase-3 (NEU3) ELISA Kit
 • Apolipoprotein AI (Apo AI) Protein
 • Activating Transcription Factor 3 (ATF3) Recombinant Protein
 • RNF26 Antibody
 • Titin (Ttn) Recombinant Protein
 • IVL Antibody
 • SHC1 Antibody
 • Prolactin (PRL) Recombinant Protein
 • IL23R Antibody
 • DEFB4A Antibody
 • Transforming Growth Factor beta (TGFbeta) ELISA Kit
 • HNE Antibody
 • Hyperglycosylated Human chorionic gonadotropin (hCG H) ELISA Kit
 downarrow more ...
separator
separator
DatasheetFull DatasheetPrinter Friendly DatasheetPrint This DatasheetAdd to Compare ListHave Questions? Ask UsRequest for a Quotation today

anti-PCNA antibody :: Rabbit PCNA Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS440048 anti-PCNA antibody
Unit / Price
0.2 mg  /  $240 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

PCNA, Polyclonal Antibody

 Also Known As   

PCNA (C19) Antibody

 Product Gene Name   

anti-PCNA antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
Table BarTOPTable Bar
 OMIM    176740
 3D Structure    ModBase 3D Structure for P12004
Section Bar
 Clonality    Polyclonal
 Host    Rabbit
 Species Reactivity    Mouse, rat, human
Section Bar
 Specificity    Mouse, rat and human PCNA
 Form/Format    200 ug/ml rabbit polyclonal IgG in 1 ml PBS containing 0.1 % sodium azide and 0.2% gelatin.
 Concentration    0.200 mg/ml (lot specific)
Section Bar
 Origin    PCNA (C19) is provided as an affinity purified rabbit polyclonal antibody, raised against a peptide mapping to the carboxy terminus of human PCNA.
 Immunogen    Synthetic peptide mapping to the carboxy terminus of human PCNA.
Section Bar
 Preparation and Storage    Store this product at 4 degree C, do not freeze. The product is stable for one year from the date of shipment.
 Other Notes    Small volumes of anti-PCNA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Table BarTOPTable Bar
 

Related Product Information for anti-PCNA antibody

   The evolutionarily conserved protein PCNA, or cyclin, was first characterized as a DNA polymerase accessory protein. It functions as a DNA sliding clamp for DNA polymerase delta and epsilon, and is an key component of for eukaryotic chromosomal DNA replication (1-3). In more recent research PCNA has been linked to an increasing number of cellular functions, these include cell cycle regulation, DNA repair, Okazaki fragment joining, DNA methylation, and chromatin assembly (2,4, &5). In cell cycle regulation p21 interacts with PCNA, which inhibits DNA replication (2,5). It has also been described that high levels of PCNA in the presence of p53 will lead to DNA repair (4). PCNA is also a very useful marker of cellular proliferation as it is seen most prominently expressed during the S phase of the cell cycle (6).
 Applications Tested/Suitable for anti-PCNA antibody   

Western Blot (WB), Immunopreciptation (IP), Immunohistochemistry (IHC)

 Application Notes for anti-PCNA antibody    Western blotting starting dilution 1:200.
Table BarTOPTable Bar
NCBI/Uniprot data below describe general gene information for PCNA. It may not necessarily be applicable to this product.
 NCBI GI #    129694
 NCBI GeneID    5111
 NCBI Accession #    P12004.1 [Other Products]
Section Bar
 UniProt Primary Accession #    P12004 [Other Products]
 UniProt Secondary Accession #    B2R897; D3DW02 [Other Products]
 UniProt Related Accession #    P12004 [Other Products]
 Molecular Weight    28,769 Da
Table BarTOPTable Bar
 NCBI Official Full Name    Proliferating cell nuclear antigen
 NCBI Official Synonym Full Names    proliferating cell nuclear antigen
 NCBI Official Symbol    PCNA [Similar Products]
 NCBI Official Synonym Symbols   
ATLD2
[Similar Products]
 NCBI Protein Information    proliferating cell nuclear antigen; DNA polymerase delta auxiliary protein; cyclin
 UniProt Protein Name    Proliferating cell nuclear antigen
 UniProt Synonym Protein Names   
Cyclin
 Protein Family    PCNA-associated factor
 UniProt Gene Name    PCNA [Similar Products]
 UniProt Synonym Gene Names    PCNA [Similar Products]
 UniProt Entry Name    PCNA_HUMAN
Section Bar
 NCBI Summary for PCNA    The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. [provided by RefSeq, Jul 2008]
Section Bar
 UniProt Comments for PCNA    PCNA: This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'- 5' exonuclease and 3'-phosphodiesterase, but not apurinic- apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Homotrimer. Forms a complex with activator 1 heteropentamer in the presence of ATP. Interacts with EXO1, POLH, POLK, DNMT1, ERCC5, FEN1, CDC6 and POLDIP2. Interacts with APEX2; this interaction is triggered by reactive oxygen species and increased by misincorporation of uracil in nuclear DNA. Forms a ternary complex with DNTTIP2 and core histone. Interacts with KCTD10 and PPP1R15A. Interacts with POLD1, POLD3 and POLD4. Interacts with BAZ1B; the interaction is direct. Interacts with HLTF and SHPRH. Interacts with NUDT15. Interaction is disrupted in response to UV irradiation and acetylation. Interacts with p21Cip1/p21(CIP1) and CDT1; interacts via their PIP-box which also recruits the DCX(DTL) complex. Interacts with DDX11. Interacts with EGFR; positively regulates PCNA. Interacts with C12orf48/PARI. Interacts with SMARCAD1. Belongs to the PCNA family.

Protein type: DNA replication; Cell cycle regulation

Chromosomal Location of Human Ortholog: 20pter-p12

Cellular Component: nucleoplasm; centrosome; PCNA complex; nuclear replication fork; cytoplasm; DNA replication factor C complex; nucleus

Molecular Function: identical protein binding; protein binding; DNA polymerase processivity factor activity; MutLalpha complex binding; purine-specific mismatch base pair DNA N-glycosylase activity; receptor tyrosine kinase binding; dinucleotide insertion or deletion binding

Biological Process: mismatch repair; positive regulation of deoxyribonuclease activity; heart development; DNA strand elongation during DNA replication; leading strand elongation; response to lipid; DNA repair; telomere maintenance via semi-conservative replication; G1/S-specific transcription in mitotic cell cycle; cell proliferation; bypass DNA synthesis; response to cadmium ion; epithelial cell differentiation; base-excision repair; nucleotide-excision repair; transcription-coupled nucleotide-excision repair; telomere maintenance via recombination; mitotic cell cycle; nucleotide-excision repair, DNA gap filling; telomere maintenance; G1/S transition of mitotic cell cycle; regulation of DNA replication

Disease: Ataxia-telangiectasia-like Disorder 2
Table BarTOPTable Bar
 

Product References and Citations for anti-PCNA antibody

   1.) Vogetseder A, Palan T, Bacic D, Kaissling B, Le Hir M. 2006. Proximal tubular epithelial cells are generated by division of differentiated cells in the healthy kidney. Am J Physiol Cell Physiol. Sept. Epub.

1. Kelman Z. 1997. PCNA: structure, functions and interactions. Oncogene 14(6):629-40.2. Tsurimoto T. 1999. PCNA binding proteins. Front Biosci 4:D849-D858.3. Balajee A.S., Geard C.R. 2001. Chromatin-bound PCNA complex formation triggered by DNA damage occurs independent of the ATM gene product in human cells. Nucleic Acid Res 29(6):1341-1351.4. Pauneshku T., Mittal S., Protic M., Oryhon J., Korolev S.V., Joachimiak A., Woloschak G.E. 2001. Proliferating cell nuclear antigen (PCNA): ringmaster of the genome. Int J Radiat Biol 77(10):1007-1021.5. Rossig L., Jadidi A.S., Urbich C., Badorff C., Zeiher A.M., Dimmeler S. 2001. Akt-dependent phosphorylation of p21(Cip1) regulates PCNA binding and proliferation of endothelial cells. Mol Cell Biol 21(16):5644-5657.6. Celis J.E., Madsen P., Nielsen S., Celis A. 1986. Nuclear patterns of cyclin (PCNA) antigen distribution subdivide S-phase in cultured cells?some applications of PCNA antibodies. Leuk Res 10(3):237-249.
Section Bar
 Research Articles on PCNA    1. Mutant p53 depletion profoundly influenced PARP1 localization and increased the level of PCNA and MCM4 proteins.
Table BarTOPTable Bar
 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Table BarTOPTable Bar
Products associated with anti-PCNA antibodyPathways associated with anti-PCNA antibody
 Reference Product  PubMed Publications
 CDKN1A antibody  >741 publications with PCNA and CDKN1A
 FEN1 antibody  >108 publications with PCNA and FEN1
 RFC1 antibody  >46 publications with PCNA and RFC1
 MSH6 antibody  >39 publications with PCNA and MSH6
 RFC4 antibody  >28 publications with PCNA and RFC4
 RFC3 antibody  >26 publications with PCNA and RFC3
 RFC5 antibody  >25 publications with PCNA and RFC5
 POLH antibody  >10 publications with PCNA and POLH
 LIG1 antibody  >9 publications with PCNA and LIG1
 POLD1 antibody  >8 publications with PCNA and POLD1
 Products by Pathway  Pathway Diagram
 BARD1 Signaling Events Pathway antibodies  BARD1 Signaling Events Pathway Diagram
 BRCA1-associated Genome Surveillance Complex (BASC) Pathway antibodies  BRCA1-associated Genome Surveillance Complex (BASC) Pathway Diagram
 BRCA1-associated Genome Surveillance Complex (BASC) Pathway antibodies  BRCA1-associated Genome Surveillance Complex (BASC) Pathway Diagram
 Base Excision Repair Pathway antibodies  Base Excision Repair Pathway Diagram
 Base Excision Repair Pathway antibodies  Base Excision Repair Pathway Diagram
 Base Excision Repair Pathway antibodies  Base Excision Repair Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle, Mitotic Pathway antibodies  Cell Cycle, Mitotic Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
Diseases associated with anti-PCNA antibodyOrgans/Tissues associated with anti-PCNA antibody
 Disease Name  Pubmed Publications
 Disease Models, Animal Antibodies  >1546 publications with PCNA and Disease Models, Animal
 Adenocarcinoma Antibodies  >1447 publications with PCNA and Adenocarcinoma
 Liver Diseases Antibodies  >1117 publications with PCNA and Liver Diseases
 Hyperplasia Antibodies  >997 publications with PCNA and Hyperplasia
 Nervous System Diseases Antibodies  >918 publications with PCNA and Nervous System Diseases
 Cardiovascular Diseases Antibodies  >856 publications with PCNA and Cardiovascular Diseases
 Neoplasms, Experimental Antibodies  >833 publications with PCNA and Neoplasms, Experimental
 Liver Neoplasms Antibodies  >816 publications with PCNA and Liver Neoplasms
 Necrosis Antibodies  >800 publications with PCNA and Necrosis
 Inflammation Antibodies  >742 publications with PCNA and Inflammation
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >2643 publications with PCNA and Blood
 Vascular Antibodies  >2070 publications with PCNA and Vascular
 Liver Antibodies  >2059 publications with PCNA and Liver
 Muscle Antibodies  >1742 publications with PCNA and Muscle
 Brain Antibodies  >1130 publications with PCNA and Brain
 Kidney Antibodies  >1031 publications with PCNA and Kidney
 Lung Antibodies  >984 publications with PCNA and Lung
 Skin Antibodies  >890 publications with PCNA and Skin
 Bone Antibodies  >845 publications with PCNA and Bone
 Lymph Antibodies  >664 publications with PCNA and Lymph
Table BarTOPTable Bar
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions