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anti-PCNA antibody :: Rabbit anti-Human PCNA Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS462104
Testing Data
Unit / Price
0.1 mg  /  $285 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

PCNA, Polyclonal Antibody

 Also Known As   

RABBIT anti PCNA

 Product Synonym Names    Rabbit anti-PCNA
 Product Gene Name   

anti-PCNA antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Chromosome Location    Chromosome: 20; NC_000020.10 (5095599..5107268, complement). Location: 20pter-p12
 OMIM    176740
 3D Structure    ModBase 3D Structure for P12004
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 Clonality    Polyclonal
 Host    Rabbit
 Species Reactivity    Human
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 Specificity    Expression of proliferating cell nuclear antigen (PCNA) or cyclin or polymerase delta auxiliary protein is elevated in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases. PCNA/cyclin may act as an auxiliary protein of DNA polymerase-delta to play a fundamental role in the initiation of cell proliferation. This antibody recognizes human PCNA. The other species are not tested.
 Purity/Purification    The Rabbit polyclonal antibody is purified by Affinity Purification
 Form/Format    This affinity purified antibody is supplied in sterile Phosphate-buffered saline (pH7.2) containing antibody stabilizer.
 Concentration    Size: 100 ug/200 ul (lot specific)
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 Antigen Preparation    Recombinant human PCNA protein
 Positive Control    Tonsil, lymph node, or small intestine
 Cellular Location    Nuclear
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 Preparation and Storage    The antibodies are stable for 12 months from date of receipt when stored at -20 degree C to -70 degree C
 Other Notes    Small volumes of anti-PCNA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Applications Tested/Suitable for anti-PCNA antibody   

Western Blot, Immunohistochemistry - Paraffin

 Application Notes for anti-PCNA antibody    Western Blot: 0.1-1 ug/ml
Immunohistochemistry: 2-5 ug/ml
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 Testing Data of anti-PCNA antibody    anti-PCNA antibody Testing Data image
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NCBI/Uniprot data below describe general gene information for PCNA. It may not necessarily be applicable to this product.
 NCBI GI #    129694
 NCBI GeneID    5111
 NCBI Accession #    P12004.1 [Other Products]
 NCBI GenBank Nucleotide #    P12004 [Other Products]
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 UniProt Primary Accession #    P12004 [Other Products]
 UniProt Secondary Accession #    B2R897; D3DW02 [Other Products]
 UniProt Related Accession #    P12004; Q6FHF5; Q6FI35; Q7Z6A0; Q7Z6A1; Q7Z6A2; Q7Z6A3 [Other Products]
 Molecular Weight    29 kDa
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 NCBI Official Full Name    Proliferating cell nuclear antigen
 NCBI Official Synonym Full Names    proliferating cell nuclear antigen
 NCBI Official Symbol    PCNA [Similar Products]
 NCBI Official Synonym Symbols   
MGC8367
[Similar Products]
 NCBI Protein Information    proliferating cell nuclear antigen; cyclin; OTTHUMP00000030189; OTTHUMP00000030190; DNA polymerase delta auxiliary protein
 UniProt Protein Name    Proliferating cell nuclear antigen
 UniProt Synonym Protein Names   
Cyclin
 Protein Family    PCNA-associated factor
 UniProt Gene Name    PCNA [Similar Products]
 UniProt Entry Name    PCNA_HUMAN
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 NCBI Summary for PCNA    The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. [provided by RefSeq]
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 UniProt Comments for PCNA    Function: This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Ref.28 Ref.30

Subunit structure: Homotrimer

By similarity. Forms a complex with activator 1 heteropentamer in the presence of ATP. Interacts with EXO1, POLH, POLK, DNMT1, ERCC5, FEN1, CDC6 and POLDIP2. Interacts with APEX2; this interaction is triggered by reactive oxygen species and increased by misincorporation of uracil in nuclear DNA. Forms a ternary complex with DNTTIP2 and core histone. Interacts with KCTD10 and PPP1R15A

By similarity. Interacts with POLD1, POLD3 and POLD4. Interacts with BAZ1B; the interaction is direct. Interacts with HLTF and SHPRH. Interacts with NUDT15. Interaction is disrupted in response to UV irradiation and acetylation. Interacts with CDKN1A/p21(CIP1) and CDT1; interacts via their PIP-box which also recruits the DCX(DTL) complex. Interacts with DDX11. Ref.10 Ref.11 Ref.12 Ref.13 Ref.14 Ref.15 Ref.16 Ref.17 Ref.18 Ref.19 Ref.20 Ref.21 Ref.22 Ref.25 Ref.26 Ref.27 Ref.28 Ref.29 Ref.30 Ref.31

Subcellular location: Nucleus. Note: Forms nuclear foci representing sites of ongoing DNA replication and vary in morphology and number during S phase. Together with APEX2, is redistributed in discrete nuclear foci in presence of oxidative DNA damaging agents.

Post-translational modification: Upon methyl methanesulfonate-induced DNA damage, mono-ubiquitinated by the UBE2B-RAD18 complex on Lys-164. This induces non-canonical polyubiquitination on Lys-164 through 'Lys-63' linkage of ubiquitin moieties by the E2 complex UBE2N-UBE2V2 and the E3 ligases, HLTF, RNF8 and SHPRH, which is required for DNA repair. 'Lys-63' polyubiquitination prevents genomic instability on DNA damage. Monoubiquitination at Lys-164 also takes place in undamaged proliferating cells, and is mediated by the DCX(DTL) complex, leading to enhance PCNA-dependent translesion DNA synthesis.Acetylated in response to UV irradiation. Acetylation disrupts interaction with NUDT15 and promotes degradation.

Miscellaneous: Antibodies against PCNA are present in sera from patients with systemic lupus erythematosus.

Sequence similarities: Belongs to the PCNA family.
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 Research Articles on PCNA    1. Treatment of HepG2 cells with HMBG1 increases expression of cyclin D1 and PCNA.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-PCNA antibodyPathways associated with anti-PCNA antibody
 Reference Product  PubMed Publications
 CCND1 antibody  >717 publications with PCNA and CCND1
 CDKN1A antibody  >658 publications with PCNA and CDKN1A
 CDK2 antibody  >304 publications with PCNA and CDK2
 CCNB1 antibody  >140 publications with PCNA and CCNB1
 FEN1 antibody  >95 publications with PCNA and FEN1
 RPA1 antibody  >58 publications with PCNA and RPA1
 MSH2 antibody  >58 publications with PCNA and MSH2
 HUS1 antibody  >50 publications with PCNA and HUS1
 DNMT1 antibody  >49 publications with PCNA and DNMT1
 RFC1 antibody  >41 publications with PCNA and RFC1
 Products by Pathway  Pathway Diagram
 BARD1 Signaling Events Pathway antibodies  BARD1 Signaling Events Pathway Diagram
 Base Excision Repair Pathway antibodies  Base Excision Repair Pathway Diagram
 Base Excision Repair Pathway antibodies  Base Excision Repair Pathway Diagram
 Base Excision Repair Pathway antibodies  Base Excision Repair Pathway Diagram
 Cell Cycle, Mitotic Pathway antibodies  Cell Cycle, Mitotic Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Chromosome Maintenance Pathway antibodies  Chromosome Maintenance Pathway Diagram
 DNA Repair Pathway antibodies  DNA Repair Pathway Diagram
Diseases associated with anti-PCNA antibodyOrgans/Tissues associated with anti-PCNA antibody
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >5907 publications with PCNA and Neoplasms
 Carcinoma Antibodies  >2947 publications with PCNA and Carcinoma
 Adenocarcinoma Antibodies  >1266 publications with PCNA and Adenocarcinoma
 Liver Diseases Antibodies  >896 publications with PCNA and Liver Diseases
 Head and Neck Neoplasms Antibodies  >768 publications with PCNA and Head and Neck Neoplasms
 Carcinoma, Squamous Cell Antibodies  >709 publications with PCNA and Carcinoma, Squamous Cell
 Drug Toxicity Antibodies  >693 publications with PCNA and Drug Toxicity
 Neoplasms, Experimental Antibodies  >676 publications with PCNA and Neoplasms, Experimental
 Intestinal Diseases Antibodies  >664 publications with PCNA and Intestinal Diseases
 Liver Neoplasms Antibodies  >659 publications with PCNA and Liver Neoplasms
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >2137 publications with PCNA and Blood
 Vascular Antibodies  >1622 publications with PCNA and Vascular
 Liver Antibodies  >1620 publications with PCNA and Liver
 Kidney Antibodies  >887 publications with PCNA and Kidney
 Brain Antibodies  >875 publications with PCNA and Brain
 Skin Antibodies  >743 publications with PCNA and Skin
 Lymph Node Antibodies  >596 publications with PCNA and Lymph Node
 Intestine Antibodies  >534 publications with PCNA and Intestine
 Stomach Antibodies  >467 publications with PCNA and Stomach
 Embryonic Tissue Antibodies  >393 publications with PCNA and Embryonic Tissue
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