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anti-PDE4D antibody :: Goat PDE4D Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS421503
Unit / Price
0.1 mg  /  $300 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

PDE4D, Polyclonal Antibody

 Also Known As   

Goat anti-PDE4D Antibody

 Product Synonym Names    Goat Anti-PDE4D3 Antibody; PDE4D; phosphodiesterase 4D, cAMP-specific (phosphodiesterase E3 dunce homolog, Drosophila); HGNC: 8783; DPDE3; HSPDE4D; PDE4DN2; STRK1; cAMP-specific phosphodiesterase 4D; cAMP-specific phosphodiesterase PDE4D6; dunce-like phosphodiesterase E3; phosphodie; PDE4D antibody; phosphodiesterase 4D; cAMP-specific (phosphodiesterase E3 dunce homolog; Drosophila) antibody; HGNC: 8783 antibody; DPDE3 antibody; HSPDE4D antibody; PDE4DN2 antibody; STRK1 antibody; cAMP-specific phosphodiesterase 4D antibody; cAMP-specific phosphodiesterase PDE4D6 antibody; dunce-like phosphodiesterase E3 antibody; phosphodiesterase 4D; cAMP-specific (dunce (Drosophila)-homolog phosphodiesterase E3) antibody; DKFZp686M11213 antibody; FLJ97311 antibody; phosphodiesterase 4D antibody
 Product Gene Name   

anti-PDE4D antibody

[Similar Products]
 Antibody/Peptide Pairs    PDE4D peptide (MBS428550) is used for blocking the activity of PDE4D antibody (MBS421503)
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Immunogen Sequence    QPEACVIDDR SPDT
 OMIM    600129
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 Clonality    Polyclonal
 Host    Goat
 Species Reactivity    Tested: Human, Mouse; Expected from sequence similarity: Human
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 Purity/Purification    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
 Form/Format    Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
 Concentration    100ug specific antibody in 200ul (lot specific)
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 Immunogen    Peptide with sequence QPEACVIDDRSPDT, from the C Terminus of the protein sequence according to NP_001184151.1; NP_001184150.1; NP_006194.2; NP_001098101.1; NP_001184152.1; NP_001159371.1.
 Epitope    C Terminus
 Note    This antiobody is expected to recognize all reported isoforms.
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 Preparation and Storage    Aliquot and store at -20 degree C. Minimize freezing and thawing.
 ISO Certification    Manufactured in an ISO 9001:2015 Certified Laboratory.
 Other Notes    Small volumes of anti-PDE4D antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Applications Tested/Suitable for anti-PDE4D antibody   

Peptide ELISA (EIA), Western Blot (WB-Trf), ELISA (EIA)

 Application Notes for anti-PDE4D antibody    Peptide ELISA: Antibody detection limit dilution 1: 128000.
Western Blot: Approx 70+80+90kDa bands observed in lysates of cell line A549. In transfected COS1 transiently expressing PDE4D1 and PDE4D3 bands of approx. 68kDa and 95kDa are observed. These bands are not observed in the untransfected COS1. In transfected HEK293 transiently expressing PDE4D2 multi bands from approx.55kDa to 80kDa are observed. These bands are not observed in the non-transfected HEK293. Calculated MW of 91.1kDa according to PDE4D1 (NP_001098101.1), 76.5kDa according to PDE4D2 (NP_006194.2) and 84.7kDa according to PDE4D3 (NP_001159371.1). Recommended concentration: 0.1-0.3ug/ml.
ELISA: This antibody has been successfully used in ELISA as described in the following paper: Bazhin et al, Mol Immunol.2010 Jan;47(4): 678-84.; PMID: 19939455.
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 Western Blot (WB) of anti-PDE4D antibody    Staining (1ug/ml) of COS cell lysates (25ug protein): transfected with Human PDE4D1 (1), transfected with Human PDE4D3 (2), untransfected (3). Primary incubation was 1 hour. Detected by chemiluminescence.
anti-PDE4D antibody Western Blot (WB) (WB) image
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 Western Blot (WB) of anti-PDE4D antibody    HEK293 overexpressing Human PDE4D2 (RC212410) and probed (mock transfection in first lane), tested by Origene.
anti-PDE4D antibody Western Blot (WB) (WB) image
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NCBI/Uniprot data below describe general gene information for PDE4D. It may not necessarily be applicable to this product.
 NCBI GI #    308387382
 NCBI GeneID    5144
 NCBI Accession #    NP_001184151.1 [Other Products]
 NCBI Related Accession #    NP_001184150.1; NP_006194.2; NP_001098101.1; NP_001184152.1; NP_001159371.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001197222.1 [Other Products]
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 UniProt Secondary Accession #    O43433; Q13549; Q13550; Q13551; Q7Z2L8; Q8IV84; Q8IVA9; Q8IVD2; Q8IVD3; Q96HL4; Q9HCX7 [Other Products]
 UniProt Related Accession #    Q08499 [Other Products]
 Molecular Weight    24,429 Da
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 NCBI Official Full Name    cAMP-specific 3',5'-cyclic phosphodiesterase 4D isoform PDE4D1
 NCBI Official Synonym Full Names    phosphodiesterase 4D
 NCBI Official Symbol    PDE4D [Similar Products]
 NCBI Official Synonym Symbols   
DPDE3; PDE43; STRK1; ACRDYS2; HSPDE4D; PDE4DN2
[Similar Products]
 NCBI Protein Information    cAMP-specific 3',5'-cyclic phosphodiesterase 4D
 UniProt Protein Name    cAMP-specific 3',5'-cyclic phosphodiesterase 4D
 UniProt Synonym Protein Names   
DPDE3; PDE43
 Protein Family    cAMP-specific 3',5'-cyclic phosphodiesterase
 UniProt Gene Name    PDE4D [Similar Products]
 UniProt Synonym Gene Names    DPDE3 [Similar Products]
 UniProt Entry Name    PDE4D_HUMAN
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 NCBI Summary for PDE4D    This gene encodes one of four mammalian counterparts to the fruit fly 'dunce' gene. The encoded protein has 3',5'-cyclic-AMP phosphodiesterase activity and degrades cAMP, which acts as a signal transduction molecule in multiple cell types. This gene uses different promoters to generate multiple alternatively spliced transcript variants that encode functional proteins.[provided by RefSeq, Sep 2009]
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 UniProt Comments for PDE4D    PDE4D: Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. Homodimer for the long isoforms. Isoforms with truncated N-termini are monomeric. Isoform 3 is part of a ternary complex containing PRKAR2A, PRKAR2B and AKAP9. Interacts with PDE4DIP. Identified in a complex composed of RYR1, PDE4D, PKA, FKBP1A and protein phosphatase 1 (PP1). Isoform 5, isoform N3 and isoform 12 bind GNB2L1 via their unique N-terminus. Binds ARRB2. Widespread; most abundant in skeletal muscle. Isoform 6 is detected in brain. Isoform 8 is detected in brain, placenta, lung and kidney. Isoform 7 is detected in heart and skeletal muscle. Inhibited by rolipram. Activated by phosphatidic acid. Belongs to the cyclic nucleotide phosphodiesterase family. PDE4 subfamily. 12 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.1.4.53; Nucleotide Metabolism - purine; Phosphodiesterase

Chromosomal Location of Human Ortholog: 5q12

Cellular Component: cytosol; voltage-gated calcium channel complex

Molecular Function: 3',5'-cyclic-AMP phosphodiesterase activity; ATPase binding; beta-2 adrenergic receptor binding; cAMP binding; drug binding; enzyme binding; protein binding

Biological Process: cAMP catabolic process; negative regulation of heart contraction; negative regulation of peptidyl-serine phosphorylation; positive regulation of interferon-gamma production; positive regulation of interleukin-2 production; positive regulation of interleukin-5 production; regulation of heart rate; regulation of receptor activity; T cell receptor signaling pathway
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 Research Articles on PDE4D    1. Data highlight an increase in PDE4D7 expression during initial tumorigenesis and further support the findings that PDE4D7 levels fall profoundly in castration resistant prostate cancer, suggesting it as a potential biomarker and therapeutic target.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-PDE4D antibodyPathways associated with anti-PDE4D antibody
 Reference Product  PubMed Publications
 GNB2L1 antibody  >6 publications with PDE4D and GNB2L1
 ADCY7 antibody  >2 publications with PDE4D and ADCY7
 ADCY3 antibody  >1 publications with PDE4D and ADCY3
 PRKACA antibody  >1 publications with PDE4D and PRKACA
 Products by Pathway  Pathway Diagram
 DARPP-32 Events Pathway antibodies  DARPP-32 Events Pathway Diagram
 G Protein Signaling Pathways antibodies  G Protein Signaling Pathways Diagram
 G Alpha (s) Signalling Events Pathway antibodies  G Alpha (s) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 Morphine Addiction Pathway antibodies  Morphine Addiction Pathway Diagram
 Morphine Addiction Pathway antibodies  Morphine Addiction Pathway Diagram
 Myometrial Relaxation And Contraction Pathways antibodies  Myometrial Relaxation And Contraction Pathways Diagram
 Opioid Signalling Pathway antibodies  Opioid Signalling Pathway Diagram
 Purine Metabolism Pathway antibodies  Purine Metabolism Pathway Diagram
 Purine Metabolism Pathway antibodies  Purine Metabolism Pathway Diagram
Diseases associated with anti-PDE4D antibodyOrgans/Tissues associated with anti-PDE4D antibody
 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >99 publications with PDE4D and Cardiovascular Diseases
 Stroke Antibodies  >87 publications with PDE4D and Stroke
 Inflammation Antibodies  >43 publications with PDE4D and Inflammation
 Lung Diseases Antibodies  >43 publications with PDE4D and Lung Diseases
 Asthma Antibodies  >41 publications with PDE4D and Asthma
 Immune System Diseases Antibodies  >31 publications with PDE4D and Immune System Diseases
 Necrosis Antibodies  >26 publications with PDE4D and Necrosis
 Heart Diseases Antibodies  >26 publications with PDE4D and Heart Diseases
 Atherosclerosis Antibodies  >16 publications with PDE4D and Atherosclerosis
 Memory Disorders Antibodies  >8 publications with PDE4D and Memory Disorders
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >113 publications with PDE4D and Brain
 Muscle Antibodies  >74 publications with PDE4D and Muscle
 Blood Antibodies  >66 publications with PDE4D and Blood
 Heart Antibodies  >56 publications with PDE4D and Heart
 Lung Antibodies  >43 publications with PDE4D and Lung
 Kidney Antibodies  >21 publications with PDE4D and Kidney
 Bone Antibodies  >12 publications with PDE4D and Bone
 Testis Antibodies  >11 publications with PDE4D and Testis
 Prostate Antibodies  >8 publications with PDE4D and Prostate
 Uterus Antibodies  >7 publications with PDE4D and Uterus
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