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anti-PDK1 antibody :: Goat PDK1 Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS421216
Western Blot (WB)
Unit / Price
0.1 mg  /  $300 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

PDK1, Polyclonal Antibody

 Also Known As   

Goat anti-PDK1 Antibody

 Product Synonym Names    PDK1; pyruvate dehydrogenase kinase, isoenzyme 1; HGNC: 8809; mitochondrial pyruvate dehydrogenase kinase isoenzyme 1; pyruvate dehydrogenase kinase, isoenzyme 1; OTTHUMP00000205076; mitochondrial pyruvate dehydrogenase, lipoamide, kinase isoenzyme 1; pyru; PDK1 antibody; pyruvate dehydrogenase kinase; isoenzyme 1 antibody; HGNC: 8809 antibody; mitochondrial pyruvate dehydrogenase kinase isoenzyme 1 antibody; pyruvate dehydrogenase kinase; isoenzyme 1 antibody; OTTHUMP00000205076 antibody; mitochondrial pyruvate dehydrogenase; lipoamide; kinase isoenzyme 1 antibody
 Product Gene Name   

anti-PDK1 antibody

[Similar Products]
 Antibody/Peptide Pairs    PDK1 peptide (MBS426632) is used for blocking the activity of PDK1 antibody (MBS421216)
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Immunogen Sequence    DFKDKSAEDA K
 OMIM    602524
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 Clonality    Polyclonal
 Host    Goat
 Species Reactivity    Tested: Human, Rat; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
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 Purity/Purification    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
 Form/Format    Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
 Concentration    100ug specific antibody in 200ul (lot specific)
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 Immunogen    Peptide with sequence C-DFKDKSAEDAK, from the internal region of the protein sequence according to NP_002601.1.
 Epitope    Internal region
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 Preparation and Storage    Aliquot and store at -20 degree C. Minimize freezing and thawing.
 ISO Certification    Manufactured in an ISO 9001:2015 Certified Laboratory.
 Other Notes    Small volumes of anti-PDK1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Applications Tested/Suitable for anti-PDK1 antibody   

Peptide ELISA (EIA), Western Blot (WB)

 Application Notes for anti-PDK1 antibody    Peptide ELISA: Antibody detection limit dilution 1: 1000.
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 Western Blot (WB) of anti-PDK1 antibody    (1ug/ml) staining of Rat Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
anti-PDK1 antibody Western Blot (WB) (WB) image
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NCBI/Uniprot data below describe general gene information for PDK1. It may not necessarily be applicable to this product.
 NCBI GI #    4505689
 NCBI GeneID    5163
 NCBI Accession #    NP_002601.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_002610.4 [Other Products]
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 UniProt Secondary Accession #    Q308M4; B2R6T1; B7Z937; D3DPD8; E9PD65 [Other Products]
 UniProt Related Accession #    Q15118 [Other Products]
 Molecular Weight    51,623 Da
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 NCBI Official Full Name    [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial isoform 2
 NCBI Official Synonym Full Names    pyruvate dehydrogenase kinase 1
 NCBI Official Symbol    PDK1 [Similar Products]
 NCBI Protein Information    [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial
 UniProt Protein Name    [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial
 UniProt Synonym Protein Names   
Pyruvate dehydrogenase kinase isoform 1; PDH kinase 1
 Protein Family    [Pyruvate dehydrogenase
 UniProt Gene Name    PDK1 [Similar Products]
 UniProt Synonym Gene Names    PDHK1; PDH kinase 1 [Similar Products]
 UniProt Entry Name    PDK1_HUMAN
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 NCBI Summary for PDK1    Pyruvate dehydrogenase (PDH) is a mitochondrial multienzyme complex that catalyzes the oxidative decarboxylation of pyruvate and is one of the major enzymes responsible for the regulation of homeostasis of carbohydrate fuels in mammals. The enzymatic activity is regulated by a phosphorylation/dephosphorylation cycle. Phosphorylation of PDH by a specific pyruvate dehydrogenase kinase (PDK) results in inactivation. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jun 2013]
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 UniProt Comments for PDK1    PDHK1: an ubiquitously expressed, atypical protein kinase associated with the mitochondrial matrix. The PDHKs play crucial roles in switching metabolic flux from oxidative phosphorylation towards glycolysis. PDHK1 is detected in heart, pancreatic islets, and skeletal muscles. Contains a HATPase_c catalytic domain, found in several ATP-binding proteins including protein histidine kinases (PHKs), PHDKs, DNA gyrase B, topoisomerases, heat shock proteins, and DNA mismatch repair proteins. PDHK regulates glucose oxidation through inhibitory phosphorylation of the E1 alpha subunit of the mitochondrial pyruvate dehydrogenase complex (PDHC) at any one of 3 inhibitory serine residues. Inhibitory sites 1, 2, and 3 correspond to S293, S300, and S232 in human PDHA1, respectively. Four PDHK isoenzymes have been described, each with different site specificity: all four phosphorylate sites 1 and 2 but at different rates; for site 1 PDHK2 >PDHK4 >PDHK1 >PDHK3; for site 2, PDHK3> PDHK4 > PDHK2 > PDHK1. Only PDHK1 phosphorylates site 3. PDHKs are recruited to the PDHC by binding to a lipoyl group covalently attached to the inner lipoyl domain of the E2 component. PDHA1 deficiency is the most common enzyme defect in patients with primary lactic acidosis. PDHK1 is a direct HIF-1 target gene. Suppression of PDH by PDHK inhibits the conversion of pyruvate to acetyl-CoA, attenuates mitochondrial respiration, and may contribute to the increased lactate production observed in many tumors. The PDH pathway is repressed in a majority of non-small cell lung carcinomas. Inhibited by AZD7545, dichloroacetate (DCA) and radicicol. Radicicol inhibits kinase activity by binding directly to the ATP-binding pocket of PDHK, similar to HSP90 from the same ATPase/kinase superfamily.

Protein type: Mitochondrial; EC 2.7.11.2; Kinase, protein; Protein kinase, atypical; ATYPICAL group; PDHK family

Chromosomal Location of Human Ortholog: 2q31.1

Cellular Component: mitochondrial matrix; mitochondrial pyruvate dehydrogenase complex; mitochondrion

Molecular Function: ATP binding; protein binding; protein kinase activity; pyruvate dehydrogenase (acetyl-transferring) kinase activity

Biological Process: cell proliferation; glucose metabolic process; induction of apoptosis by oxidative stress; protein amino acid phosphorylation; regulation of acetyl-CoA biosynthetic process from pyruvate
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 Research Articles on PDK1    1. Decreased PDK1 level is closely associated with reduced Akt/cyclin D1 activity.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-PDK1 antibodyPathways associated with anti-PDK1 antibody
 Reference Product  PubMed Publications
 PDPK1 antibody  >345 publications with PDK1 and PDPK1
 MTOR antibody  >187 publications with PDK1 and MTOR
 RPS6KB1 antibody  >4 publications with PDK1 and RPS6KB1
 PDHA1 antibody  >3 publications with PDK1 and PDHA1
 PDHB antibody  >1 publications with PDK1 and PDHB
 Products by Pathway  Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Central Carbon Metabolism In Cancer Pathway antibodies  Central Carbon Metabolism In Cancer Pathway Diagram
 Central Carbon Metabolism In Cancer Pathway antibodies  Central Carbon Metabolism In Cancer Pathway Diagram
 DNA Damage Response (only ATM Dependent) Pathway antibodies  DNA Damage Response (only ATM Dependent) Pathway Diagram
 EPO Receptor Signaling Pathway antibodies  EPO Receptor Signaling Pathway Diagram
 ErbB Signaling Pathway antibodies  ErbB Signaling Pathway Diagram
 HIF-1 Signaling Pathway antibodies  HIF-1 Signaling Pathway Diagram
 Integrated Pancreatic Cancer Pathway antibodies  Integrated Pancreatic Cancer Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
Diseases associated with anti-PDK1 antibodyOrgans/Tissues associated with anti-PDK1 antibody
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >57 publications with PDK1 and Inflammation
 Nervous System Diseases Antibodies  >54 publications with PDK1 and Nervous System Diseases
 Breast Neoplasms Antibodies  >52 publications with PDK1 and Breast Neoplasms
 Disease Models, Animal Antibodies  >46 publications with PDK1 and Disease Models, Animal
 Brain Diseases Antibodies  >44 publications with PDK1 and Brain Diseases
 Insulin Resistance Antibodies  >42 publications with PDK1 and Insulin Resistance
 Cell Transformation, Neoplastic Antibodies  >38 publications with PDK1 and Cell Transformation, Neoplastic
 Lung Neoplasms Antibodies  >35 publications with PDK1 and Lung Neoplasms
 Necrosis Antibodies  >33 publications with PDK1 and Necrosis
 Neoplasms, Experimental Antibodies  >26 publications with PDK1 and Neoplasms, Experimental
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >141 publications with PDK1 and Blood
 Muscle Antibodies  >114 publications with PDK1 and Muscle
 Brain Antibodies  >87 publications with PDK1 and Brain
 Vascular Antibodies  >67 publications with PDK1 and Vascular
 Heart Antibodies  >63 publications with PDK1 and Heart
 Lung Antibodies  >57 publications with PDK1 and Lung
 Liver Antibodies  >55 publications with PDK1 and Liver
 Kidney Antibodies  >48 publications with PDK1 and Kidney
 Prostate Antibodies  >35 publications with PDK1 and Prostate
 Bone Antibodies  >26 publications with PDK1 and Bone
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