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anti-PDK4 antibody :: Rabbit PDK4 Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS855252
Testing Data
Unit / Price
0.1 mg  /  $330 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

PDK4, Polyclonal Antibody

 Also Known As   

PDK4 Antibody

 Product Gene Name   

anti-PDK4 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    602527
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 Clonality    Polyclonal
 Host    Rabbit
 Species Reactivity    Human, Mouse, Rat
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 Form/Format    Lyophilized anti-PDK4 antibody purified by peptide affinity column containing 2% BSA and 0.02% NaN3
 Concentration    100ug/200ul (lot specific)
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 Source Note    Rabbit polyclonal PDK4 (1) antibody was raised against a peptide at the C terminal residues of human PDK4 protein.
 Reactivity Note    Not yet tested in other species.
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 Other Notes    Small volumes of anti-PDK4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Product Categories/Family for anti-PDK4 antibody    Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells; Autophagy antibody
 Applications Tested/Suitable for anti-PDK4 antibody   

Western Blot (WB), Immunohistochemistry (IHC)

 Application Notes for anti-PDK4 antibody    Peptide ELISA titer: 1:40,000-1:80,000
Immunohistochemistry: 1:50-1:200
Optimal dilutions/concentrations should be determined by the end user.
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 Testing Data of anti-PDK4 antibody    anti-PDK4 antibody Testing Data image
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NCBI/Uniprot data below describe general gene information for PDK4. It may not necessarily be applicable to this product.
 NCBI GI #    94421466
 NCBI GeneID    5166
 NCBI Accession #    NP_002603.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_002612 [Other Products]
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 UniProt Related Accession #    Q16654 [Other Products]
 Molecular Weight    46,469 Da
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 NCBI Official Full Name    Homo sapiens pyruvate dehydrogenase kinase, isozyme 4 (PDK4), mRNA
 NCBI Official Synonym Full Names    pyruvate dehydrogenase kinase, isozyme 4
 NCBI Official Symbol    PDK4 [Similar Products]
 NCBI Protein Information    pyruvate dehydrogenase kinase, isozyme 4; pyruvate dehydrogenase kinase, isoenzyme 4; pyruvate dehydrogenase, lipoamide, kinase isozyme 4, mitochondrial; [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 4, mitochondrial; [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 4, mitochondrial
 UniProt Protein Name    [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 4, mitochondrial
 UniProt Synonym Protein Names   
Pyruvate dehydrogenase kinase isoform 4
 UniProt Gene Name    PDK4 [Similar Products]
 UniProt Synonym Gene Names    PDHK4 [Similar Products]
 UniProt Entry Name    PDK4_HUMAN
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 NCBI Summary for PDK4    This gene is a member of the PDK/BCKDK protein kinase family and encodes a mitochondrial protein with a histidine kinase domain. This protein is located in the matrix of the mitrochondria and inhibits the pyruvate dehydrogenase complex by phosphorylating one of its subunits, thereby contributing to the regulation of glucose metabolism. Expression of this gene is regulated by glucocorticoids, retinoic acid and insulin. [provided by RefSeq, Jul 2008]
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 UniProt Comments for PDK4    PDHK4: an ubiquitously expressed, atypical protein kinase associated with the mitochondrial matrix. The PDHKs play crucial roles in switching metabolic flux from oxidative phosphorylation towards glycolysis. PDHK4 is abundant in heart, skeletal muscle, kidney, and pancreatic islets. Contains a HATPase_c catalytic domain, found in several ATP-binding proteins including protein histidine kinases (PHKs), PHDKs, DNA gyrase B, topoisomerases, heat shock proteins, and DNA mismatch repair proteins. PDHK regulates glucose oxidation through inhibitory phosphorylation of the E1 alpha subunit of the mitochondrial pyruvate dehydrogenase complex (PDHC) at any one of 3 inhibitory serine residues. Inhibitory sites 1, 2, and 3 correspond to S293, S300, and S232 in human PDHA1, respectively. Four PDHK isoenzymes have been described, each with different site specificity: all four phosphorylate sites 1 and 2 but at different rates; for site 1 PDHK2 >PDHK4 >PDHK1 >PDHK3; for site 2, PDHK3> PDHK4 > PDHK2 > PDHK1. Only PDHK1 phosphorylates site 3. PDHKs are recruited to the PDHC by binding to a lipoyl group covalently attached to the inner lipoyl domain of the E2 component. PDHA1 deficiency is the most common enzyme defect in patients with primary lactic acidosis. Suppression of PDH by PDHK inhibits the conversion of pyruvate to acetyl-CoA, attenuates mitochondrial respiration, and may contribute to the increased lactate production observed in many tumors. The PDH pathway is repressed in a majority of non-small cell lung carcinomas. Inhibited by AZD7545, dichloroacetate (DCA), and radicicol. Radicicol inhibits kinase activity by binding directly to the ATP-binding pocket of PDHK, similar to HSP90 from the same ATPase/kinase superfamily.

Protein type: EC 2.7.11.2; Mitochondrial; Protein kinase, atypical; Kinase, protein; ATYPICAL group; PDHK family

Chromosomal Location of Human Ortholog: 7q21.3

Cellular Component: mitochondrion; mitochondrial matrix; mitochondrial inner membrane

Molecular Function: pyruvate dehydrogenase (acetyl-transferring) kinase activity; ATP binding; protein kinase activity

Biological Process: response to starvation; cellular metabolic process; regulation of bone resorption; regulation of fatty acid biosynthetic process; insulin receptor signaling pathway; regulation of fatty acid oxidation; regulation of pH; glucose metabolic process; regulation of acetyl-CoA biosynthetic process from pyruvate; pyruvate metabolic process; cellular response to starvation; glucose homeostasis; protein amino acid phosphorylation
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Product References and Citations for anti-PDK4 antibody

   (1) Wynn,R.M., Kato,M., Chuang,J.L., et al.Pyruvate dehydrogenase kinase-4 structures reveal a metastable open conformation fostering robust core-free basal activity. J. Biol. Chem. 283 (37), 25305-25315 (2008)
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 Research Articles on PDK4    1. Data show that the orphan nuclear receptor estrogen related receptor gamma (ERRgamma) plays a critical role in hypoxia-mediated activation of pyruvate dehydrogenase kinase 4 (PDK4) gene expression.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products related to PDK4 Antibody
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 MBS2510681  Chicken PDK4 (Pyruvate Dehydrogenase Kinase Isozyme 4) ELISA Kit  MBS2511158  Monkey PDK4 (Pyruvate Dehydrogenase Kinase Isozyme 4) ELISA Kit
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 MBS9226525  PDK4 Antibody (Center D98) Blocking Peptide  MBS2049989  PE-Linked Polyclonal Antibody to Pyruvate Dehydrogenase Kinase Isozyme 4 (PDK4)
Products associated with anti-PDK4 antibodyPathways associated with anti-PDK4 antibody
 Reference Product  PubMed Publications
 FOXO1 antibody  >23 publications with PDK4 and FOXO1
 UCP3 antibody  >21 publications with PDK4 and UCP3
 FOXO3 antibody  >3 publications with PDK4 and FOXO3
 INS antibody  >2 publications with PDK4 and INS
 PDHX antibody  >1 publications with PDK4 and PDHX
 PDHA1 antibody  >1 publications with PDK4 and PDHA1
 Products by Pathway  Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Pyruvate Metabolism Pathway antibodies  Pyruvate Metabolism Pathway Diagram
 Pyruvate Metabolism And Citric Acid (TCA) Cycle Pathway antibodies  Pyruvate Metabolism And Citric Acid (TCA) Cycle Pathway Diagram
 Regulation Of Pyruvate Dehydrogenase (PDH) Complex Pathway antibodies  Regulation Of Pyruvate Dehydrogenase (PDH) Complex Pathway Diagram
 The Citric Acid (TCA) Cycle And Respiratory Electron Transport Pathway antibodies  The Citric Acid (TCA) Cycle And Respiratory Electron Transport Pathway Diagram
Diseases associated with anti-PDK4 antibodyOrgans/Tissues associated with anti-PDK4 antibody
 Disease Name  Pubmed Publications
 Insulin Resistance Antibodies  >51 publications with PDK4 and Insulin Resistance
 Fatty Liver Antibodies  >39 publications with PDK4 and Fatty Liver
 Inflammation Antibodies  >22 publications with PDK4 and Inflammation
 Heart Diseases Antibodies  >20 publications with PDK4 and Heart Diseases
 Cardiovascular Diseases Antibodies  >20 publications with PDK4 and Cardiovascular Diseases
 Hypertrophy Antibodies  >14 publications with PDK4 and Hypertrophy
 Liver Diseases Antibodies  >13 publications with PDK4 and Liver Diseases
 Necrosis Antibodies  >12 publications with PDK4 and Necrosis
 Hyperglycemia Antibodies  >11 publications with PDK4 and Hyperglycemia
 Weight Loss Antibodies  >10 publications with PDK4 and Weight Loss
 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >177 publications with PDK4 and Muscle
 Liver Antibodies  >71 publications with PDK4 and Liver
 Heart Antibodies  >58 publications with PDK4 and Heart
 Adipose Tissue Antibodies  >38 publications with PDK4 and Adipose Tissue
 Connective Tissue Antibodies  >23 publications with PDK4 and Connective Tissue
 Brain Antibodies  >17 publications with PDK4 and Brain
 Kidney Antibodies  >14 publications with PDK4 and Kidney
 Pancreas Antibodies  >11 publications with PDK4 and Pancreas
 Thyroid Antibodies  >9 publications with PDK4 and Thyroid
 Nerve Antibodies  >7 publications with PDK4 and Nerve
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