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| Product Name
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PHAP III (ANP32E), Polyclonal Antibody
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| Also Known As
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PHAP III Antibody
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| Product Synonym Names
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PHAP III; LANPL; LANP-L; Acidic leucine-rich nuclear phosphoprotein 32 family member E; LANP-like protein; acidic (leucine-rich) nuclear phosphoprotein 32 family, member E
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| Product Gene Name
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[Similar Products]
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| Antibody/Peptide Pairs
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PHAP III peptide (MBS152716) is used for blocking the activity of PHAP III antibody (MBS151145)
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| Research Use Only
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For Research Use Only. Not for use in diagnostic procedures.
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 TOP
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| OMIM
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609611
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| 3D Structure
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ModBase 3D Structure for Q9BTT0
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| Clonality
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Polyclonal
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| Isotype
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IgG
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| Host
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Rabbit
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| Species Reactivity
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Human, Mouse, Rat
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| Specificity
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PHAP III has no cross-reaction to PHAP I and PHAP I2a.
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| Purity/Purification
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PHAP III Antibody is affinity chromatography purified via peptide column.
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| Form/Format
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Liquid
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| Concentration
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1 mg/mL (lot specific)
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| Conjugate
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Unconjugated
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| Immunogen
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PHAP III antibody was raised with a synthetic peptide corresponding to amino acids close to carboxy terminus of human PHAP III.
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| Buffer
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PHAP III Antibody is supplied in PBS containing 0.02% sodium azide.
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| Preparation and Storage
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PHAP III antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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| Other Notes
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Small volumes of anti-ANP32E antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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TOP
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PHAP III Antibody: Apoptosis is related to many diseases and development. Caspase-9 plays a central role in cell death induced by a variety of apoptosis activators. Cytochrome c, after released from mitochondria, binds to Apaf-1, which forms an apoptosome that in turn binds to and activate procaspase-9. Activated caspase-9 cleaves and activates the effector caspases (caspase-3, -6 and -7), which are responsible for the proteolytic cleavage of many key proteins in apoptosis. The tumor suppressor putative HLA-DR-associated proteins (PHAPs) were recently identified as important regulators of mitochondrion apoptosis. PHAP appears to facilitate apoptosome-medicated caspase-9 activation and to stimulate the mitochondrial apoptotic pathway. PHAP was also shown to oppose both Ras- and Myc-medicated cell transformation.
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| Applications Tested/Suitable for anti-ANP32E antibody
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ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
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| Application Notes for anti-ANP32E antibody
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PHAP III antibody can be used for detection of PHAP III by Western blot at 1 mug/mL. A band at approximately 35 kDa can be detected. Antibody can also be used for immunohistochemistry starting at 2 mug/mL. For immunofluorescence start at 10 mug/mL.
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| Western Blot (WB) of anti-ANP32E antibody
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Western blot analysis of PHAP III expression in human A549 (A) and HepG2 (B) cells, and rat testis (C) with PHAP antibody III at 1 μg/mL.
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| Immunohistochemistry (IHC) of anti-ANP32E antibody
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Immunohistochemistry of PHAP III in human small intestine tissue with PHAP III antibody at 2 μg/mL.
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| Immunofluorescence (IF) of anti-ANP32E antibody
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Immunofluorescence of PHAP III in Human Small Intestine cells with PHAP III antibody at 10 μg/mL.
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TOP
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| NCBI/Uniprot data below describe general gene information for ANP32E. It may not necessarily be applicable to this product. |
| NCBI GI #
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13569879
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| NCBI GeneID
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81611
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| NCBI Accession #
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NP_112182
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[Other Products]
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| NCBI GenBank Nucleotide #
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NM_030920.4
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[Other Products]
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| UniProt Primary Accession #
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Q9BTT0
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[Other Products]
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| UniProt Secondary Accession #
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Q5TB18; Q5TB20; Q8N1S4; Q8WWW9; B4E0I6; E9PEA6
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[Other Products]
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| UniProt Related Accession #
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Q9BTT0
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[Other Products]
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| Molecular Weight
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35 kDa
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TOP
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| NCBI Official Full Name
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acidic leucine-rich nuclear phosphoprotein 32 family member E isoform 1
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| NCBI Official Synonym Full Names
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acidic (leucine-rich) nuclear phosphoprotein 32 family, member E
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| NCBI Official Symbol
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ANP32E
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[Similar Products]
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| NCBI Official Synonym Symbols
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LANPL; LANP-L
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[Similar Products]
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| NCBI Protein Information
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acidic leucine-rich nuclear phosphoprotein 32 family member E; LANP-like protein; leucine-rich acidic nuclear protein like
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| UniProt Protein Name
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Acidic leucine-rich nuclear phosphoprotein 32 family member E
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| UniProt Synonym Protein Names
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LANP-like protein; LANP-L
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| Protein Family
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Acidic leucine-rich nuclear phosphoprotein 32 family
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| UniProt Gene Name
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ANP32E
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[Similar Products]
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| UniProt Synonym Gene Names
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LANP-L
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[Similar Products]
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| UniProt Entry Name
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AN32E_HUMAN
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| UniProt Comments for ANP32E
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LANP-L: Inhibits activity of protein phosphatase 2A (PP2A). Does not inhibit protein phosphatase 1. May play a role in cerebellar development and synaptogenesis process by modulating PP2A activity. Belongs to the ANP32 family.
Protein type: Inhibitor; Protein phosphatase, regulatory subunit; Vesicle; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 1q21.2
Cellular Component: cytoplasmic membrane-bound vesicle; nucleus
Molecular Function: phosphatase inhibitor activity; histone binding
Biological Process: negative regulation of catalytic activity; histone exchange
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TOP
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| Research Articles on ANP32E
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1. 1.48 A resolution crystal structure of the complex formed between the ANP32E-ZID and the H2A.Z/H2B dimer and biochemical data support an underlying molecular mechanism for H2A.Z/H2B eviction from the nucleosome and its stabilization by ANP32E
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TOP
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| Precautions
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All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
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| Disclaimer
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While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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TOP
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| Diseases associated with anti-ANP32E antibody | Organs/Tissues associated with anti-ANP32E antibody |
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TOP
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Antibody 
